FastQCFastQC Report
Fri 27 May 2016
ERR765379_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765379_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8028772
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA592760.7382947230286275No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT542330.6754831249411491No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG349000.4346866494651984No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA315370.39279979553535704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294340.36660649972374354No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC288180.35893409353260997No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG282340.35166025389685995No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG248360.30933746779706783No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT227260.28305698555146414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214070.2666285703467479No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC193750.24131959407989165No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC187100.233036882850827No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC174110.2168575717432255No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT172460.21480246294202898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171280.21333274876905212No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA139640.1739244806055023No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG113850.14180250728255828No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG113590.14147867195630914No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC107800.1342671083448378No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC94180.11730311933132488No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC93570.11654335183512497No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC88920.11075168157720758No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT87740.10928196740423068No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG81340.10131063629655941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC22500.026.951273
TATCACG4650.023.2657782
GATCTAC39550.021.4076351
AGTACTT346550.021.24188812-13
CGATCTC25600.021.1095225
GAGTACT335800.019.15805412-13
TACTTTT393900.018.85698914-15
TCTCGCG11500.018.7966548
CGTTAAC9500.018.3198031
TCTACAC54550.018.1827563
CCGATCT35950.017.648024
ATCTACA50550.017.4936072
GTACTTT373000.017.2991814-15
CATGGGT79900.017.2338374
GTACTAG22700.016.9914591
TACACTC52300.016.981685
CATGGGG218200.016.7781414
GTATTAG32200.016.3608111
GATGTAA31800.015.9749421
ACTTTTT410650.015.94335216-17
Produced by FastQC (version 0.11.5)