##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765379_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8028772 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5005399331305 34.0 31.0 34.0 31.0 34.0 2 32.64398117171592 34.0 31.0 34.0 31.0 34.0 3 32.68694278527277 34.0 31.0 34.0 31.0 34.0 4 36.19192324803843 37.0 35.0 37.0 35.0 37.0 5 36.13543254186319 37.0 35.0 37.0 35.0 37.0 6 36.183563563643354 37.0 37.0 37.0 35.0 37.0 7 36.14059348054721 37.0 36.0 37.0 35.0 37.0 8 36.14853105804973 37.0 37.0 37.0 35.0 37.0 9 37.870715845461795 39.0 38.0 39.0 35.0 39.0 10-11 37.83101712192101 39.0 38.0 39.0 35.0 39.0 12-13 37.79306580134546 39.0 38.0 39.0 35.0 39.0 14-15 39.14700566911104 40.5 38.5 41.0 36.0 41.0 16-17 39.14548133886477 40.0 39.0 41.0 36.0 41.0 18-19 39.19978372782288 40.0 39.0 41.0 36.0 41.0 20-21 39.17883052352215 40.5 39.0 41.0 36.0 41.0 22-23 39.091490890512276 40.0 39.0 41.0 35.5 41.0 24-25 39.01596856904144 40.0 38.5 41.0 35.0 41.0 26-27 38.881386344013755 40.0 38.0 41.0 35.0 41.0 28-29 38.74173578475015 40.0 38.0 41.0 35.0 41.0 30-31 38.54894465803737 40.0 38.0 41.0 34.5 41.0 32-33 38.2987951955791 40.0 38.0 41.0 34.0 41.0 34-35 38.16786390247475 40.0 38.0 41.0 34.0 41.0 36-37 38.01110767624239 40.0 37.5 41.0 33.5 41.0 38-39 37.810422627520126 40.0 37.0 41.0 33.0 41.0 40-41 37.56315617132981 40.0 37.0 41.0 33.0 41.0 42-43 37.26067012489581 40.0 36.5 41.0 32.5 41.0 44-45 36.8797422569728 40.0 36.0 41.0 31.5 41.0 46-47 36.46812140137993 39.0 35.5 41.0 31.0 41.0 48-49 36.09216253743412 39.0 35.0 41.0 30.0 41.0 50-51 35.73413854572031 39.0 35.0 41.0 29.0 41.0 52-53 35.53797124890332 39.0 35.0 41.0 28.5 41.0 54-55 35.4822919370484 39.0 35.0 41.0 28.5 41.0 56-57 35.27337804585807 38.5 35.0 41.0 28.0 41.0 58-59 35.09755638844894 38.0 35.0 41.0 28.0 41.0 60-61 34.77789075340537 37.0 35.0 40.0 28.0 41.0 62-63 34.444878183114426 37.0 35.0 40.0 27.0 41.0 64-65 34.17455029237348 36.0 35.0 39.0 27.0 41.0 66-67 33.82504740450968 36.0 34.0 39.0 26.0 41.0 68-69 33.4792636408158 35.0 34.0 39.0 26.0 41.0 70-71 33.12605931517298 35.0 34.0 37.5 26.0 40.0 72-73 32.77837544770234 35.0 34.0 37.0 26.0 39.0 74-75 32.44757118523231 35.0 34.0 36.5 25.0 39.0 76-77 31.078794490614506 34.0 31.5 35.0 22.0 37.0 78-79 31.842419114654145 35.0 33.0 35.0 24.0 37.0 80-81 31.798415560935098 35.0 33.0 35.0 24.0 37.0 82-83 31.632119395095536 35.0 33.0 35.0 24.0 36.5 84-85 31.456435616804164 35.0 33.0 35.0 23.0 36.0 86-87 31.296932146036777 35.0 33.0 35.0 22.5 36.0 88-89 31.153771460940725 35.0 33.0 35.0 20.0 36.0 90-91 31.02779329142738 35.0 33.0 35.0 19.5 35.0 92-93 30.891422997190602 35.0 33.0 35.0 18.5 35.0 94-95 30.778606491752413 35.0 33.0 35.0 15.0 35.0 96-97 30.65334026174862 35.0 33.0 35.0 4.5 35.0 98-99 30.518959063727305 35.0 33.0 35.0 2.0 35.0 100 30.203302198642582 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.43273952910314506 1101 2 0.36745045830636514 1101 3 0.3964973891797996 1101 4 0.3106515478966969 1101 5 0.33065006194141944 1101 6 0.3097851652388428 1101 7 0.3042029122448042 1101 8 0.307840336704416 1101 9 0.4082351085333116 1101 10-11 0.43762559689590574 1101 12-13 0.4618721470362459 1101 14-15 0.591787675018324 1101 16-17 0.5346727711013557 1101 18-19 0.4743353301162827 1101 20-21 0.48311260858498173 1101 22-23 0.5217662819184739 1101 24-25 0.5710991755966646 1101 26-27 0.5860672037534798 1101 28-29 0.5868204868378797 1101 30-31 0.6361066923037342 1101 32-33 0.6956844897047105 1101 34-35 0.6446781699483068 1101 36-37 0.7623324175773192 1101 38-39 0.7645735795431818 1101 40-41 0.8463269037800814 1101 42-43 0.8807554478993254 1101 44-45 0.979371362117881 1101 46-47 1.0337858045152402 1101 48-49 1.1033047068584736 1101 50-51 1.2399527025790604 1101 52-53 1.1462592834714584 1101 54-55 1.2651982751586814 1101 56-57 1.2595781392618264 1101 58-59 1.309607037996635 1101 60-61 1.324499167884376 1101 62-63 1.310183249505961 1101 64-65 1.2389540038456346 1101 66-67 1.2791603150538506 1101 68-69 1.3434160489384936 1101 70-71 1.358747925381664 1101 72-73 1.4179586180041355 1101 74-75 1.366066535782899 1101 76-77 1.3730395614926074 1101 78-79 1.3855173681341775 1101 80-81 1.3606617550487137 1101 82-83 1.3368470665975174 1101 84-85 1.3519807365119938 1101 86-87 1.4021043143697973 1101 88-89 1.4452184802143329 1101 90-91 1.4606116075116553 1101 92-93 1.4892844799789415 1101 94-95 1.489430921944951 1101 96-97 1.5373646958729204 1101 98-99 1.5479671632724923 1101 100 1.5926736250382802 1102 1 0.41473136606006733 1102 2 0.34588789085625393 1102 3 0.3657812723874869 1102 4 0.23799794961772136 1102 5 0.2651513702709778 1102 6 0.26923175836423496 1102 7 0.30789970646468845 1102 8 0.2933140349699741 1102 9 0.34091329835225537 1102 10-11 0.39036549242398877 1102 12-13 0.3622556946867235 1102 14-15 0.45214228839511605 1102 16-17 0.4805277873153031 1102 18-19 0.5039682524455245 1102 20-21 0.47630785594903813 1102 22-23 0.5181537367329767 1102 24-25 0.4933109493311676 1102 26-27 0.5345143134697281 1102 28-29 0.5925187498092015 1102 30-31 0.5622641313093979 1102 32-33 0.5786126200541375 1102 34-35 0.6344677029229473 1102 36-37 0.7002521184861195 1102 38-39 0.6715427727086265 1102 40-41 0.7737992318159286 1102 42-43 0.789290267873568 1102 44-45 0.8550512883821497 1102 46-47 0.902101076613441 1102 48-49 0.9080472183975061 1102 50-51 0.9512351637034939 1102 52-53 1.0555892515102343 1102 54-55 1.0714908833721566 1102 56-57 1.1063825029962473 1102 58-59 1.0761186755507808 1102 60-61 1.0836987307590604 1102 62-63 0.9996041167952612 1102 64-65 1.0802563183930758 1102 66-67 1.1525913793412244 1102 68-69 1.1302036121268841 1102 70-71 1.1130293876773365 1102 72-73 1.0808683792380478 1102 74-75 1.0329002486193062 1102 76-77 1.0120305175875721 1102 78-79 1.0706197302572384 1102 80-81 1.074020846626599 1102 82-83 1.089557535243305 1102 84-85 1.1039566220245653 1102 86-87 1.1218373982876209 1102 88-89 1.114870497608603 1102 90-91 1.1187072733985737 1102 92-93 1.1533306114782107 1102 94-95 1.1985158820209705 1102 96-97 1.2292631122010818 1102 98-99 1.227607960857668 1102 100 1.2471128387571717 1103 1 0.33401107965516275 1103 2 0.2939539662195614 1103 3 0.3304956850772669 1103 4 0.18853556015173467 1103 5 0.2836128436168792 1103 6 0.2475575043915086 1103 7 0.2720374330112847 1103 8 0.24969527044383 1103 9 0.31233800732049843 1103 10-11 0.3283688756617025 1103 12-13 0.3075034807684105 1103 14-15 0.42689690073940056 1103 16-17 0.4527511235605246 1103 18-19 0.4223826824122838 1103 20-21 0.39040653001497105 1103 22-23 0.4144426293378487 1103 24-25 0.4573048281410337 1103 26-27 0.45663735295309493 1103 28-29 0.5539287698639299 1103 30-31 0.5728571137971841 1103 32-33 0.5295879722376569 1103 34-35 0.5053072375866776 1103 36-37 0.6346532602016381 1103 38-39 0.592724990866401 1103 40-41 0.6903870187163577 1103 42-43 0.7475578989063791 1103 44-45 0.7459466598825983 1103 46-47 0.7584887649401821 1103 48-49 0.7845149822341924 1103 50-51 0.8198085790134968 1103 52-53 0.9649549198812366 1103 54-55 1.026023677163721 1103 56-57 0.9310942466565493 1103 58-59 0.9430647345576162 1103 60-61 1.04168522324958 1103 62-63 1.0354886283062044 1103 64-65 0.9646651040794936 1103 66-67 0.8937771639144572 1103 68-69 0.8755488964213143 1103 70-71 0.8068790325044475 1103 72-73 0.9341423347605513 1103 74-75 0.9264626489788199 1103 76-77 0.910920673147583 1103 78-79 0.9862925535006433 1103 80-81 0.9423272433466074 1103 82-83 0.966075869556132 1103 84-85 0.976647258148649 1103 86-87 0.9396067164292283 1103 88-89 0.9973850471728802 1103 90-91 1.0722230721334007 1103 92-93 1.02407645256827 1103 94-95 1.0765798133718647 1103 96-97 1.145808342652348 1103 98-99 1.0805225919267905 1103 100 1.1085109013748244 1104 1 0.2897329344238173 1104 2 0.2206855453950709 1104 3 0.255303224713856 1104 4 0.20468412025429217 1104 5 0.23603699805307343 1104 6 0.23250750477746607 1104 7 0.21728706887301286 1104 8 0.15755460054816695 1104 9 0.23009869202514466 1104 10-11 0.24670864858667585 1104 12-13 0.3147824757052149 1104 14-15 0.40337377610346437 1104 16-17 0.3767973128601696 1104 18-19 0.3038558970956231 1104 20-21 0.3393869725387262 1104 22-23 0.3929871213048344 1104 24-25 0.38734268783334613 1104 26-27 0.412190454199866 1104 28-29 0.4245613224301792 1104 30-31 0.4217277952775831 1104 32-33 0.4174297916405152 1104 34-35 0.4273099179293851 1104 36-37 0.45863463793579484 1104 38-39 0.5039628353578252 1104 40-41 0.5274377985630778 1104 42-43 0.5434446060335105 1104 44-45 0.5160693297984551 1104 46-47 0.4579324483509737 1104 48-49 0.4908586183203667 1104 50-51 0.44990932047205234 1104 52-53 0.4864198987751749 1104 54-55 0.5137979832821387 1104 56-57 0.4958540341529911 1104 58-59 0.5671415631314645 1104 60-61 0.5672672358579973 1104 62-63 0.5853304441463507 1104 64-65 0.5085241266035183 1104 66-67 0.541250344158307 1104 68-69 0.5252668191497776 1104 70-71 0.49602465396809237 1104 72-73 0.4475891462274717 1104 74-75 0.4716046020425395 1104 76-77 0.4627690348667777 1104 78-79 0.5239913896238768 1104 80-81 0.46756328748378806 1104 82-83 0.4949337609209046 1104 84-85 0.5151858538367087 1104 86-87 0.5129607906510891 1104 88-89 0.5317541186069157 1104 90-91 0.5497466112308338 1104 92-93 0.5830985609422328 1104 94-95 0.571811962283487 1104 96-97 0.6293065402198543 1104 98-99 0.6071548012856631 1104 100 0.6741690846439994 1105 1 0.20960756496576494 1105 2 0.11836736128822878 1105 3 0.17570012375247757 1105 4 0.1454684632910812 1105 5 0.16893446131888368 1105 6 0.15917819322429239 1105 7 0.15379053028305378 1105 8 0.10036208247699818 1105 9 0.1706916408583652 1105 10-11 0.17965122396665123 1105 12-13 0.21871822229427806 1105 14-15 0.23065059310527403 1105 16-17 0.24320838069909456 1105 18-19 0.23333411750898847 1105 20-21 0.2770292346722698 1105 22-23 0.3079202162085224 1105 24-25 0.28717787094578995 1105 26-27 0.2752669784185784 1105 28-29 0.33299962808276007 1105 30-31 0.36159121007749917 1105 32-33 0.35089870108030397 1105 34-35 0.40406566004216415 1105 36-37 0.3557463367813867 1105 38-39 0.3926538407788911 1105 40-41 0.3993756085818134 1105 42-43 0.3654997023367983 1105 44-45 0.3997692309844254 1105 46-47 0.5063610195859454 1105 48-49 0.5525563276465775 1105 50-51 0.55235080059974 1105 52-53 0.5659948014075411 1105 54-55 0.5995959339672723 1105 56-57 0.6764672764010342 1105 58-59 0.6510789713891754 1105 60-61 0.6716232834926359 1105 62-63 0.6485528178368654 1105 64-65 0.6140664325930203 1105 66-67 0.7201138826972908 1105 68-69 0.6872244951779791 1105 70-71 0.6460850588543963 1105 72-73 0.6161729344087092 1105 74-75 0.6503005403468833 1105 76-77 0.6271087660413173 1105 78-79 0.6987608539504855 1105 80-81 0.6637262141972151 1105 82-83 0.6480323663597751 1105 84-85 0.6637882852311101 1105 86-87 0.6810403708181063 1105 88-89 0.6983915966150853 1105 90-91 0.7297760542476404 1105 92-93 0.7042728790508654 1105 94-95 0.7410045424848022 1105 96-97 0.7637730867334902 1105 98-99 0.7558316376979306 1105 100 0.7393666444304507 1106 1 0.18968551327566985 1106 2 0.11125499485594759 1106 3 0.15788653855869939 1106 4 0.13675844474368404 1106 5 0.16822217125024963 1106 6 0.164286401663702 1106 7 0.18275817790850368 1106 8 0.17139352360625537 1106 9 0.17777195213717079 1106 10-11 0.18811003609960153 1106 12-13 0.2004435372894804 1106 14-15 0.2974288776259115 1106 16-17 0.2492692282308866 1106 18-19 0.19774593355557357 1106 20-21 0.23534341408199566 1106 22-23 0.2595868935387031 1106 24-25 0.2859220165987182 1106 26-27 0.3193434768790624 1106 28-29 0.389130512247263 1106 30-31 0.26774233228900357 1106 32-33 0.22689758910254199 1106 34-35 0.26546111522890214 1106 36-37 0.24877675473083372 1106 38-39 0.2442995853183163 1106 40-41 0.24386127556586956 1106 42-43 0.22806696403372229 1106 44-45 0.26866673921971795 1106 46-47 0.25989106104889714 1106 48-49 0.258226554697238 1106 50-51 0.2174571051181431 1106 52-53 0.27887504677940456 1106 54-55 0.28721631447874785 1106 56-57 0.3329003514771074 1106 58-59 0.2712590089001665 1106 60-61 0.2388904414174604 1106 62-63 0.15150407312895453 1106 64-65 0.20617853179130918 1106 66-67 0.1821240826988273 1106 68-69 0.18885825781124765 1106 70-71 0.16806090232338988 1106 72-73 0.16445156620428492 1106 74-75 0.19336621896514572 1106 76-77 0.14326382017930328 1106 78-79 0.25264029981770264 1106 80-81 0.27614347587151045 1106 82-83 0.22926884995855445 1106 84-85 0.24564666142690683 1106 86-87 0.23972891370521765 1106 88-89 0.26921055925474136 1106 90-91 0.30393831769304924 1106 92-93 0.3053999085185346 1106 94-95 0.35096927793833643 1106 96-97 0.4174584825929877 1106 98-99 0.41336824051473897 1106 100 0.4308050296107311 1107 1 0.12615149963223615 1107 2 0.07589079053047953 1107 3 0.07729758877879789 1107 4 0.07346126452023327 1107 5 0.17595169991572845 1107 6 0.1564862807687888 1107 7 0.0681249123229577 1107 8 0.10403989963069904 1107 9 0.09315768877120689 1107 10-11 0.11154404988342748 1107 12-13 0.14639673168095158 1107 14-15 0.162494928818667 1107 16-17 0.12265321400672491 1107 18-19 0.1468262294240006 1107 20-21 0.25155516525576616 1107 22-23 0.156557198961643 1107 24-25 0.15075294347865764 1107 26-27 0.19498104251716342 1107 28-29 0.20946240803835536 1107 30-31 0.2608811063574237 1107 32-33 0.20704780497192843 1107 34-35 0.25091975688058454 1107 36-37 0.247579616728423 1107 38-39 0.17812983850484443 1107 40-41 0.1593540450354496 1107 42-43 0.15444213759202086 1107 44-45 0.17081496788581063 1107 46-47 0.08937902536371922 1107 48-49 0.08676272944563124 1107 50-51 0.061777861070886786 1107 52-53 0.021270450794936835 1107 54-55 0.007430086549653936 1107 56-57 0.08910120587198378 1107 58-59 0.08335486991263252 1107 60-61 0.046681259886284465 1107 62-63 0.05058292846420187 1107 64-65 0.00514091147397977 1107 66-67 -0.029252872908884342 1107 68-69 -0.0808568425716345 1107 70-71 -0.007875244334599074 1107 72-73 -0.04402688100838503 1107 74-75 -0.03223395690197606 1107 76-77 -0.10017969379871872 1107 78-79 0.010000920755860676 1107 80-81 0.01736545574641113 1107 82-83 -0.01568826152339753 1107 84-85 0.018390269167770867 1107 86-87 0.023902358660340184 1107 88-89 0.05514122578272662 1107 90-91 0.0599672694799267 1107 92-93 0.07010665350381373 1107 94-95 0.08532919258796667 1107 96-97 0.14014411192561127 1107 98-99 0.08226716898390762 1107 100 0.12846437073893568 1108 1 0.08978046744027068 1108 2 0.0316875382279207 1108 3 0.05703297911246352 1108 4 0.08228089659301219 1108 5 0.11448996370410214 1108 6 0.08546600911090252 1108 7 0.10191849754156834 1108 8 0.05852764551713108 1108 9 0.11244854240701585 1108 10-11 0.1358250526000191 1108 12-13 0.12563510483717977 1108 14-15 0.12724139969275683 1108 16-17 0.08897289226005256 1108 18-19 0.14282951266238086 1108 20-21 0.14757038495312713 1108 22-23 0.08924852876951661 1108 24-25 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-0.5892869675294534 2316 50-51 -0.5991887343277611 2316 52-53 -0.6676923772268779 2316 54-55 -0.7429095971941777 2316 56-57 -0.6892309075582617 2316 58-59 -0.6816004011305665 2316 60-61 -0.570409804644612 2316 62-63 -0.5347567750351416 2316 64-65 -0.5895287046911761 2316 66-67 -0.5861624756205259 2316 68-69 -0.5609744718504928 2316 70-71 -0.560457660641589 2316 72-73 -0.5158453786903223 2316 74-75 -0.4764240943559166 2316 76-77 -0.5055363030805502 2316 78-79 -0.5729738129274402 2316 80-81 -0.5660137597719448 2316 82-83 -0.5706556254115966 2316 84-85 -0.5615611544887678 2316 86-87 -0.579640274483193 2316 88-89 -0.5602209224517978 2316 90-91 -0.557333450460451 2316 92-93 -0.6115054681842338 2316 94-95 -0.6323244349445041 2316 96-97 -0.6017365069751826 2316 98-99 -0.588615905033592 2316 100 -0.6360745281182574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30.0 3 39.0 4 72.0 5 171.0 6 371.0 7 950.0 8 1997.0 9 3726.0 10 6511.0 11 11994.0 12 19702.0 13 27974.0 14 32920.0 15 45141.0 16 62349.0 17 85824.0 18 86843.0 19 52910.0 20 33972.0 21 28047.0 22 29299.0 23 32716.0 24 36249.0 25 40404.0 26 47787.0 27 59319.0 28 73694.0 29 93018.0 30 116347.0 31 147176.0 32 185522.0 33 243663.0 34 348394.0 35 571489.0 36 1127581.0 37 2147748.0 38 1876937.0 39 349614.0 40 272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80956390342134 19.02598554299462 13.864249725860942 23.300200827723096 2 16.04454829735905 22.453765643731522 36.351397683449655 25.150288375459777 3 19.08254313644658 26.98311667779813 29.793689811764267 24.140650373991022 4 13.852428417490081 18.11810116098733 34.92431043216999 33.1051599893526 5 14.06146543954667 37.364568330001156 32.50000124552049 16.073964984931692 6 31.1805466639232 35.40906380203598 17.391065532811247 16.019324001229577 7 26.144994527182984 32.78126468157272 21.715599845156895 19.358140946087396 8 27.90906255651549 31.112080402831214 20.724800754087923 20.254056286565365 9 28.061040966165184 14.236485978179477 19.6996875736414 38.002785482013934 10-11 25.660031338562405 25.61825032525233 25.655460275249002 23.06625806093626 12-13 27.403127792889077 23.863098170632938 26.18451503192803 22.549259004549953 14-15 25.40606146056639 23.56252844857441 25.140808182702106 25.89060190815709 16-17 23.295830114166208 26.46852249290143 26.382300918463724 23.853346474468637 18-19 24.247799040749943 25.258713287660928 28.623237775340986 21.870249896248144 20-21 25.300711399138947 24.77104506482806 27.531196499490257 22.397047036542737 22-23 24.164195430904297 25.22219946958113 26.44927265453071 24.164332444983867 24-25 23.8531508466797 25.931590547179717 26.250091079332122 23.96516752680846 26-27 24.256172386657834 25.30973096407881 26.60618562659007 23.82791102267329 28-29 23.66522956059879 24.724998234393563 28.313607326388574 23.29616487861907 30-31 24.75770999733204 24.72081689938049 27.49950115848313 23.02197194480434 32-33 23.008971520673345 25.665758277804823 27.460560626159943 23.86470957536189 34-35 23.1386127354677 26.102405273913458 27.628770419531357 23.13021157108749 36-37 24.104016979100034 26.027225525298537 26.71497274352231 23.15378475207912 38-39 24.31402645512498 25.054900241647633 26.730558812559202 23.90051449066819 40-41 23.673472565132762 25.736503336936035 26.840349882406205 23.749674215524998 42-43 25.19770913611573 25.231525796506908 26.48850289596413 23.082262171413227 44-45 24.695310640471973 25.34417246219244 26.680021587417517 23.280495309918066 46-47 24.040657829648918 25.546571646524026 27.16304004421187 23.24973047961519 48-49 24.774802069631956 26.106805518977033 26.376772134662463 22.741620276728547 50-51 23.77979447793502 26.72446982528893 25.319185957947727 24.17654973882832 52-53 23.625469468246557 26.700623499669668 24.584323173520644 25.08958385856313 54-55 24.279570128218847 26.121994323834898 24.544449943526107 25.05398560442015 56-57 24.59504270817741 25.171085925714685 24.943185226533082 25.29068613957482 58-59 24.05990405946676 25.26582923178116 26.160387150893076 24.513879557859 60-61 24.200737202685016 25.678581099427806 25.567800738872116 24.552880959015067 62-63 22.8529342541059 25.937170669049458 25.94564090305898 25.26425417378566 64-65 22.777100861061314 25.921977591081074 25.79571068698563 25.505210860871983 66-67 23.960580204310556 25.835415639629446 25.19899715497715 25.00500700108286 68-69 23.95228327271518 25.193908134009195 25.284763746138374 25.569044847137256 70-71 23.206892765768288 25.636469267909245 25.452269094171452 25.70436887215102 72-73 24.172625490271788 25.34064002809916 25.439061816904584 25.04767266472447 74-75 24.241827305944156 25.188838251073648 25.47057056632511 25.098763876657088 76-77 23.282865350033862 25.850613814524483 25.86171151885037 25.004809316591288 78-79 23.37890642636972 25.906396165495497 25.67549886017965 25.039198547955127 80-81 22.848282402339983 25.884045530250454 25.512263892909154 25.75540817450041 82-83 22.927149401184106 26.049855281280433 24.76080744550971 26.262187872025756 84-85 23.14267650677794 25.905247515466563 24.46664003743537 26.48543594032013 86-87 23.06391259091879 25.23261147994621 25.037007716412468 26.66646821272253 88-89 23.13989518690788 24.809067842040026 25.46390679117892 26.587130179873174 90-91 23.434876997981 25.345166670195663 25.0020426642819 26.217913667541442 92-93 22.646548778888363 25.67131278515637 25.13349904698252 26.54863938897275 94-95 22.099915004327006 25.82173278576058 25.559982482720617 26.518369727191786 96-97 22.95280526059146 25.65055852370635 24.815453952698768 26.581182263003416 98-99 22.911959637164937 25.45402335500373 24.768731756238687 26.865285251592645 100 22.928623740770636 25.756023376079874 24.896758138283563 26.41859474486593 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 855.0 1 554.0 2 309.5 3 399.0 4 348.5 5 407.5 6 689.0 7 1409.0 8 2274.0 9 2404.0 10 1907.5 11 1668.5 12 1893.5 13 2221.0 14 2694.5 15 3265.0 16 3931.0 17 4752.0 18 5562.5 19 6571.5 20 7914.0 21 9514.0 22 11481.5 23 13847.5 24 16776.5 25 20412.5 26 25750.5 27 31986.5 28 38151.0 29 47392.0 30 58332.5 31 67798.0 32 77693.5 33 87870.5 34 98324.0 35 106469.0 36 113534.0 37 124521.5 38 132591.0 39 139824.5 40 143334.5 41 144578.0 42 151679.0 43 169958.5 44 196979.5 45 223683.0 46 257119.0 47 296812.0 48 384734.0 49 467807.0 50 536100.0 51 515023.0 52 406329.0 53 365305.0 54 339264.0 55 303866.0 56 277699.5 57 250288.0 58 231956.5 59 214182.0 60 184249.5 61 151815.0 62 123252.0 63 97635.5 64 72809.5 65 55893.0 66 42302.0 67 30560.5 68 22944.5 69 18192.5 70 14942.5 71 12518.0 72 10754.5 73 10987.5 74 8750.0 75 6516.5 76 4851.5 77 3044.0 78 2101.5 79 1299.5 80 878.5 81 664.0 82 474.5 83 299.0 84 190.5 85 114.0 86 78.0 87 40.0 88 15.5 89 11.5 90 5.5 91 2.0 92 3.0 93 2.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.06266213562920955 3 0.024599029590079282 4 0.0064642513201271625 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.850362670654989E-5 12-13 1.4323485584096795E-4 14-15 3.7988374809995847E-4 16-17 4.857529893737174E-4 18-19 0.0 20-21 0.0028273315022521502 22-23 0.004982081942294538 24-25 7.099466767769716E-4 26-27 3.736561456720903E-4 28-29 0.004595970591766712 30-31 0.0021173848254751786 32-33 0.0011022856297326663 34-35 0.0015319901972555704 36-37 0.0039047067222733435 38-39 0.004315728482512643 40-41 9.279127617523576E-4 42-43 0.0023042128983112235 44-45 2.6155930197046325E-4 46-47 0.0019741499696342103 48-49 1.8682807283604516E-5 50-51 0.00180600470408177 52-53 0.006725810622097626 54-55 0.014560134476355787 56-57 0.02529029345957265 58-59 0.02786852086471007 60-61 0.02588814329264799 62-63 0.008201752397502381 64-65 0.006725810622097626 66-67 1.743728679803088E-4 68-69 0.0012330652807178981 70-71 8.095883156228624E-5 72-73 7.722227010556533E-4 74-75 0.005063040773856824 76-77 0.001052464810309721 78-79 0.0011770168588670844 80-81 0.0 82-83 0.0011707892564392164 84-85 1.2455204855736343E-5 86-87 5.106633990851901E-4 88-89 4.982081942294537E-5 90-91 5.231186039409265E-4 92-93 7.846779059113897E-4 94-95 0.0015942662215342519 96-97 2.1173848254751784E-4 98-99 0.0 100 6.476706524982899E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8028772.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.28660973619422 #Duplication Level Percentage of deduplicated Percentage of total 1 74.75718917744246 26.379277594792544 2 13.666869277097938 9.645149649930756 3 4.442207908977473 4.702513705533703 4 2.010176815436334 2.837292991481906 5 1.1102776769039373 1.9588967541858788 6 0.6924128758065479 1.4659741754940945 7 0.49514927497882316 1.2230497459136076 8 0.35714910270952255 1.0082064803954296 9 0.2709272903655714 0.8604095005813042 >10 1.8209055097630416 12.267521944433527 >50 0.18499552693036794 4.550098028466109 >100 0.15383402396933088 11.356115863526016 >500 0.023399887090842297 5.752319423762601 >1k 0.013164497306692339 8.455550656821096 >5k 6.705776105769893E-4 1.649930064537848 >10k+ 6.705776105769893E-4 5.887693420143328 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 59276 0.7382947230286275 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 54233 0.6754831249411491 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 34900 0.4346866494651984 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 31537 0.39279979553535704 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29434 0.36660649972374354 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 28818 0.35893409353260997 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 28234 0.35166025389685995 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 24836 0.30933746779706783 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 22726 0.28305698555146414 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21407 0.2666285703467479 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 19375 0.24131959407989165 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 18710 0.233036882850827 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 17411 0.2168575717432255 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 17246 0.21480246294202898 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17128 0.21333274876905212 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 13964 0.1739244806055023 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 11385 0.14180250728255828 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 11359 0.14147867195630914 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 10780 0.1342671083448378 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9418 0.11730311933132488 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 9357 0.11654335183512497 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 8892 0.11075168157720758 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8774 0.10928196740423068 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 8134 0.10131063629655941 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0036244646130192764 0.0 0.0 0.0 1.2455204855736345E-5 2 0.006215147223012436 0.0 0.0 1.2455204855736345E-5 1.2455204855736345E-5 3 0.006476706524982899 0.0 0.0 1.2455204855736345E-5 1.2455204855736345E-5 4 0.007522943732864752 0.0 0.0 1.2455204855736345E-5 1.2455204855736345E-5 5 0.030303513414006524 0.0 0.0 1.4946245826883613E-4 1.2455204855736345E-5 6 0.05924940949873779 0.0 0.0 3.6120094081635397E-4 2.491040971147269E-5 7 0.06552683274602891 0.0 0.0 4.3593216995077203E-4 2.491040971147269E-5 8 0.07512979568980163 0.0 0.0 5.355738087966628E-4 2.491040971147269E-5 9 0.08209225520415825 0.0 0.0 6.103050379310809E-4 3.736561456720903E-5 10-11 0.15028450178931474 0.0 6.227602427868172E-6 0.0013700725341309979 3.736561456720903E-5 12-13 0.277252860088691 0.0 1.2455204855736345E-5 0.0038175202882831894 6.850362670654989E-5 14-15 0.42798574925281224 0.0 1.2455204855736345E-5 0.006744493429381231 8.71864339901544E-5 16-17 0.4956361944267442 0.0 1.2455204855736345E-5 0.007946420697959786 1.120968437016271E-4 18-19 0.5270544486753391 0.0 1.2455204855736345E-5 0.008569180940746605 1.120968437016271E-4 20-21 0.6517808701006829 0.0 1.2455204855736345E-5 0.011191001562879105 1.120968437016271E-4 22-23 0.6864748432263365 0.0 1.2455204855736345E-5 0.013239882761647734 1.2455204855736345E-4 24-25 0.8455278092340897 0.0 2.491040971147269E-5 0.025520714749403768 1.2455204855736345E-4 26-27 1.1845771682145165 0.0 2.491040971147269E-5 0.06378933166865369 1.2455204855736345E-4 28-29 1.2596695982897508 0.0 2.491040971147269E-5 0.0904559252647852 1.2455204855736345E-4 30-31 1.2795418776370782 0.0 2.491040971147269E-5 0.15603257883023705 1.2455204855736345E-4 32-33 1.2976081522803238 0.0 2.491040971147269E-5 0.2528904793908707 1.6191766312457248E-4 34-35 1.3111967807779321 0.0 2.491040971147269E-5 0.37551197119559504 1.6191766312457248E-4 36-37 1.3324266774545348 0.0 2.491040971147269E-5 0.570853176550536 1.6191766312457248E-4 38-39 1.352635247332967 0.0 2.491040971147269E-5 0.8481807678683615 1.6191766312457248E-4 40-41 1.3712121853752977 0.0 2.491040971147269E-5 1.2182684973492832 1.6191766312457248E-4 42-43 1.4066161051777284 0.0 2.491040971147269E-5 1.5060958263604944 1.6191766312457248E-4 44-45 1.4481168477570416 0.0 2.491040971147269E-5 1.796694438452107 1.6191766312457248E-4 46-47 1.4662578536294217 0.0 2.491040971147269E-5 2.135407506901429 1.6191766312457248E-4 48-49 1.4787006032803025 0.0 2.491040971147269E-5 2.454933332270489 1.743728679803088E-4 50-51 1.4993326501238298 0.0 3.113801213934086E-5 2.81155698530236 1.743728679803088E-4 52-53 1.5061207367702059 0.0 3.736561456720903E-5 3.2101671339029183 1.8060047040817698E-4 54-55 1.5265410451311858 0.0 6.227602427868172E-5 3.563813992974268 1.8682807283604516E-4 56-57 1.5692188045693662 0.0 6.227602427868172E-5 3.942850787144036 1.8682807283604516E-4 58-59 1.5818234718833715 0.0 6.227602427868172E-5 4.332486960646037 1.8682807283604516E-4 60-61 1.5897325269667641 0.0 6.227602427868172E-5 4.696658467820484 1.8682807283604516E-4 62-63 1.595829349743647 0.0 6.227602427868172E-5 5.080471833052427 1.8682807283604516E-4 64-65 1.6009608941442104 0.0 6.227602427868172E-5 5.486611152988278 1.8682807283604516E-4 66-67 1.6076306563444573 0.0 6.227602427868172E-5 5.895733494487077 1.8682807283604516E-4 68-69 1.61495431679963 0.0 6.227602427868172E-5 6.36563723568187 1.8682807283604516E-4 70-71 1.624544824538547 0.0 6.227602427868172E-5 6.8995420470278646 1.8682807283604516E-4 72-73 1.6488573844169445 6.227602427868172E-6 6.227602427868172E-5 7.371855870362243 2.1173848254751787E-4 74-75 1.6720738862680369 1.2455204855736345E-5 6.227602427868172E-5 7.831060590585958 2.1173848254751787E-4 76-77 1.6792169462528017 1.2455204855736345E-5 6.227602427868172E-5 8.334811350976214 2.1173848254751787E-4 78-79 1.684784422823316 1.2455204855736345E-5 6.227602427868172E-5 8.852898052155423 2.1173848254751787E-4 80-81 1.6971773516547737 1.2455204855736345E-5 6.227602427868172E-5 9.416819907203742 2.1173848254751787E-4 82-83 1.7019601503193762 1.2455204855736345E-5 6.227602427868172E-5 10.040738484042143 2.241936874032542E-4 84-85 1.719553127178104 1.8682807283604516E-5 6.227602427868172E-5 10.636526980713864 2.241936874032542E-4 86-87 1.7495328052658614 2.491040971147269E-5 6.850362670654989E-5 11.236799351133648 2.241936874032542E-4 88 1.7561340638394016 2.491040971147269E-5 7.473122913441806E-5 11.711654036258595 2.241936874032542E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 2250 0.0 26.95127 3 TATCACG 465 0.0 23.265778 2 GATCTAC 3955 0.0 21.407635 1 AGTACTT 34655 0.0 21.241888 12-13 CGATCTC 2560 0.0 21.109522 5 GAGTACT 33580 0.0 19.158054 12-13 TACTTTT 39390 0.0 18.856989 14-15 TCTCGCG 1150 0.0 18.796654 8 CGTTAAC 950 0.0 18.319803 1 TCTACAC 5455 0.0 18.182756 3 CCGATCT 3595 0.0 17.64802 4 ATCTACA 5055 0.0 17.493607 2 GTACTTT 37300 0.0 17.29918 14-15 CATGGGT 7990 0.0 17.233837 4 GTACTAG 2270 0.0 16.991459 1 TACACTC 5230 0.0 16.98168 5 CATGGGG 21820 0.0 16.778141 4 GTATTAG 3220 0.0 16.360811 1 GATGTAA 3180 0.0 15.974942 1 ACTTTTT 41065 0.0 15.943352 16-17 >>END_MODULE