FastQCFastQC Report
Fri 27 May 2016
ERR765376_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765376_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6941206
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA103160.14861970671955277No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT88840.12798928601168152No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA295800.022.6999681
GTACAAG36850.022.5876871
GTATAAG23450.021.812531
GTACTAG22450.021.1271061
GTACATG433000.020.8338761
TACATGG424000.020.56282
ATGGGTA43450.019.5843035
AACGCAG313500.019.2375286
GTGATCG15800.018.5408788
CATGGGG171550.018.458794
TAGGCAT30300.018.415585
TAGTACT25100.018.1551234
TATCAAC375650.017.9486372
ACATGGG446100.017.8659213
CAACGCA337950.017.8319725
CATGGGT57000.017.7839584
CGCAGAG329850.017.6636898
ATCAACG341550.017.5895633
ACGCAGA336700.017.5252957
TGATCGC17100.017.4032639
Produced by FastQC (version 0.11.5)