FastQCFastQC Report
Fri 27 May 2016
ERR765376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765376_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6941206
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212420.30602751164567077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139520.20100253471803026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113370.163328966176771No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA102260.14732310206612512No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT99500.1433468477956136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG7000.041.8504522
GTATCAA374300.034.631091
GGTATCA236100.027.9437791
ATCAACG484150.026.4701823
CAACGCA484800.026.4538765
AACGCAG504500.025.8255146
TCAACGC500500.025.7076724
TATCAAC515550.025.1105442
TCACGCA12550.024.0839274
ATCACGC12600.023.2502523
ACGCAGA555450.023.1636097
CGCAGAG560750.022.9529678
AGTACTT332700.022.02347412-13
GCAGAGT594300.021.51739
AGAGTAC571850.019.97363510-11
ATGGGTA40100.018.6697775
GAGTACT322700.018.52649912-13
TACTTTT398500.018.4922814-15
CATGGGG142700.018.2808574
CAGAGTA582450.017.4043839
Produced by FastQC (version 0.11.5)