FastQCFastQC Report
Fri 27 May 2016
ERR765375_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765375_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6543674
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA188400.022.9788381
GTACATG302700.019.0795651
AACGCAG213900.018.8257876
TACATGG298450.018.5872382
ATCAACG216350.018.3331953
CAACGCA218050.018.2115865
TATTCGC4400.017.9656229
GTACTAG18450.017.6425321
TATCAAC244800.017.551192
GTATAAG20300.017.4091191
TCAACGC229000.017.340774
ACGCAGA227350.017.1393787
CGCAGAG227650.016.9942388
ACATGGG301850.016.9298273
GTACAAG30250.016.7556151
CATGGGG144300.016.0797844
GCAGAGT237100.015.8266139
GTATTAC12000.015.5002251
CTAACGC9900.015.4997533
TAGTACT23850.015.4022684
Produced by FastQC (version 0.11.5)