##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765375_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6543674 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.425375561190855 33.0 33.0 33.0 27.0 33.0 2 31.248140417753085 33.0 33.0 33.0 27.0 33.0 3 31.330852820602004 33.0 33.0 33.0 27.0 33.0 4 34.95333004058576 37.0 37.0 37.0 33.0 37.0 5 35.02161186513876 37.0 37.0 37.0 33.0 37.0 6 35.028326288870744 37.0 37.0 37.0 33.0 37.0 7 34.88022737685282 37.0 37.0 37.0 33.0 37.0 8 34.81015588490502 37.0 37.0 37.0 33.0 37.0 9 34.774050938356645 37.0 37.0 37.0 33.0 37.0 10-11 34.734606048528704 37.0 37.0 37.0 30.0 37.0 12-13 34.68347063133035 37.0 37.0 37.0 27.0 37.0 14-15 36.08955282613407 37.0 37.0 40.0 27.0 40.0 16-17 36.13001633333201 37.0 37.0 40.0 27.0 40.0 18-19 36.128011725523 37.0 37.0 40.0 27.0 40.0 20-21 36.13136763842453 37.0 37.0 40.0 27.0 40.0 22-23 36.16358837558228 37.0 37.0 40.0 27.0 40.0 24-25 36.10582732269364 37.0 37.0 40.0 27.0 40.0 26-27 36.11193665821372 37.0 37.0 40.0 27.0 40.0 28-29 36.03512078994155 37.0 37.0 40.0 27.0 40.0 30-31 35.99957989960991 37.0 37.0 40.0 27.0 40.0 32-33 35.87923909412358 37.0 37.0 40.0 27.0 40.0 34-35 35.8296428734072 37.0 37.0 40.0 27.0 40.0 36-37 35.71899608690775 37.0 37.0 40.0 27.0 40.0 38-39 35.51397983762639 37.0 33.0 40.0 27.0 40.0 40-41 35.44554473526646 37.0 33.0 40.0 27.0 40.0 42-43 35.318330573925294 37.0 33.0 40.0 27.0 40.0 44-45 35.178158630763086 37.0 33.0 40.0 27.0 40.0 46-47 35.050834057442344 37.0 33.0 40.0 27.0 40.0 48-49 34.88375574638957 37.0 33.0 40.0 27.0 40.0 50-51 34.71288056220405 37.0 33.0 40.0 27.0 40.0 52-53 34.525148104871974 37.0 33.0 40.0 27.0 40.0 54-55 34.397088623302444 37.0 33.0 40.0 27.0 40.0 56-57 34.199755443195976 37.0 33.0 38.5 27.0 40.0 58-59 33.966818029137755 37.0 33.0 37.0 27.0 40.0 60-61 33.7353337742681 37.0 33.0 37.0 27.0 40.0 62-63 33.5644175580874 37.0 33.0 37.0 24.5 40.0 64-65 33.25411175740112 37.0 33.0 37.0 22.0 40.0 66-67 33.02985799720463 37.0 33.0 37.0 22.0 40.0 68-69 32.73582195567811 37.0 33.0 37.0 22.0 38.5 70-71 32.59914315719273 35.0 33.0 37.0 22.0 37.0 72-73 32.32740941862324 33.0 33.0 37.0 22.0 37.0 74-75 32.05376597306039 33.0 33.0 37.0 22.0 37.0 76-77 31.87251817556926 33.0 33.0 37.0 22.0 37.0 78-79 31.634375505258973 33.0 33.0 37.0 22.0 37.0 80-81 31.35556477905226 33.0 27.0 37.0 22.0 37.0 82-83 31.176477617925343 33.0 27.0 37.0 22.0 37.0 84-85 30.99578019931922 33.0 27.0 37.0 22.0 37.0 86-87 30.78529569168635 33.0 27.0 37.0 18.5 37.0 88-89 30.599335862391676 33.0 27.0 37.0 15.0 37.0 90-91 30.39398234080732 33.0 27.0 37.0 15.0 37.0 92-93 30.248198565515334 33.0 27.0 37.0 15.0 37.0 94-95 29.99745807936031 33.0 27.0 37.0 15.0 37.0 96-97 29.90904505633991 33.0 27.0 37.0 15.0 37.0 98-99 28.75584327703367 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.345460004818289 1101 2 0.29768071525435147 1101 3 0.34243218843213086 1101 4 0.36107350767156987 1101 5 0.3139146600263629 1101 6 0.2874011811358059 1101 7 0.2966708193512986 1101 8 0.32261931365302843 1101 9 0.25620166764834806 1101 10-11 0.3288825173692018 1101 12-13 0.32956753882737644 1101 14-15 0.46188971686461144 1101 16-17 0.461171967255531 1101 18-19 0.38234611423461473 1101 20-21 0.3705399968913454 1101 22-23 0.30101224447539465 1101 24-25 0.3584319259650073 1101 26-27 0.47103464665367767 1101 28-29 0.34532192887004953 1101 30-31 0.4393160803095455 1101 32-33 0.5035674554264844 1101 34-35 0.4368353733766739 1101 36-37 0.3923047576205221 1101 38-39 0.3637346577607019 1101 40-41 0.41151606677608044 1101 42-43 0.40096866507037987 1101 44-45 0.3894316128116202 1101 46-47 0.4598011915451039 1101 48-49 0.4561846951241719 1101 50-51 0.4371780955975879 1101 52-53 0.3407116906493002 1101 54-55 0.48000819004681716 1101 56-57 0.4382325937896283 1101 58-59 0.3729887369837357 1101 60-61 0.44405235879093397 1101 62-63 0.412943910299191 1101 64-65 0.27997916382517474 1101 66-67 0.36193923157725294 1101 68-69 0.385318248529785 1101 70-71 0.3152433203514988 1101 72-73 0.4120322132713099 1101 74-75 0.3703072723137737 1101 76-77 0.3726461099832399 1101 78-79 0.42000462614574374 1101 80-81 0.4101429312469129 1101 82-83 0.36531385572796893 1101 84-85 0.31265511051736183 1101 86-87 0.35664924961255906 1101 88-89 0.42152217622326305 1101 90-91 0.4691154450777191 1101 92-93 0.5683825495843138 1101 94-95 0.4856716778282504 1101 96-97 0.42546232687008967 1101 98-99 0.48649359538623926 1102 1 0.19217264766512443 1102 2 0.2251392559436347 1102 3 0.24325361862934258 1102 4 0.24246561616637052 1102 5 0.26312600545537634 1102 6 0.2304465186710729 1102 7 0.16373381736119086 1102 8 0.19752774770505255 1102 9 0.22376767232080397 1102 10-11 0.2003567411138718 1102 12-13 0.2332759538066469 1102 14-15 0.29346122786695616 1102 16-17 0.32963421616642563 1102 18-19 0.3205524571611491 1102 20-21 0.3389399971052782 1102 22-23 0.26722939538761636 1102 24-25 0.37424184843794706 1102 26-27 0.36448915651792646 1102 28-29 0.21730949377668196 1102 30-31 0.2410913514775288 1102 32-33 0.3114599829683655 1102 34-35 0.23858911776595448 1102 36-37 0.36107120675873716 1102 38-39 0.32454321837948896 1102 40-41 0.24868625785880027 1102 42-43 0.27942916160070297 1102 44-45 0.2667064703219353 1102 46-47 0.29428641838438807 1102 48-49 0.28873330383498796 1102 50-51 0.3362801810539011 1102 52-53 0.24191447292528778 1102 54-55 0.2829496613376534 1102 56-57 0.3271683520194415 1102 58-59 0.30395637401752396 1102 60-61 0.3138211638617676 1102 62-63 0.29078154445252835 1102 64-65 0.17860262557843498 1102 66-67 0.21664774078890048 1102 68-69 0.19056188692442078 1102 70-71 0.18246025591365367 1102 72-73 0.19488389290207664 1102 74-75 0.18144987032422932 1102 76-77 0.1873224783669265 1102 78-79 0.22975638649215924 1102 80-81 0.26751271804581833 1102 82-83 0.21208363247624717 1102 84-85 0.18259090497140562 1102 86-87 0.2651021851318447 1102 88-89 0.29135110816858756 1102 90-91 0.17959021720498924 1102 92-93 0.34793410101503497 1102 94-95 0.2291827682539065 1102 96-97 0.1870837686853868 1102 98-99 0.2459143843384659 1103 1 0.14387169192663762 1103 2 0.23030504502427362 1103 3 0.15750912822939256 1103 4 0.23677950306662154 1103 5 0.20416651256825702 1103 6 0.17708023406274975 1103 7 0.0342893185423776 1103 8 0.13045317551831204 1103 9 0.1857468529247157 1103 10-11 0.14201519154861586 1103 12-13 0.1577320441412553 1103 14-15 0.14410422412847623 1103 16-17 0.10569477194417232 1103 18-19 0.1428579760292834 1103 20-21 0.21151865248130264 1103 22-23 0.18121635262603064 1103 24-25 0.3165328540334613 1103 26-27 0.3141423091377433 1103 28-29 0.2301512229402718 1103 30-31 0.3907761186181702 1103 32-33 0.32122578258175594 1103 34-35 0.26346626733037226 1103 36-37 0.2994679689137172 1103 38-39 0.25793178548208573 1103 40-41 0.25802887417993503 1103 42-43 0.33969218325404427 1103 44-45 0.27761263486431176 1103 46-47 0.24551415701872514 1103 48-49 0.3156105850383284 1103 50-51 0.22616705344793075 1103 52-53 0.2045223902651614 1103 54-55 0.3348181357260387 1103 56-57 0.25301130442305464 1103 58-59 0.3003366193163828 1103 60-61 0.33656629848598385 1103 62-63 0.304616440814776 1103 64-65 0.2801835898897522 1103 66-67 0.3155206985985757 1103 68-69 0.30954720091644106 1103 70-71 0.24380454346258773 1103 72-73 0.31230473206844067 1103 74-75 0.30114795694516516 1103 76-77 0.25785070886732697 1103 78-79 0.2320264960379781 1103 80-81 0.21230789753602153 1103 82-83 0.22520377062868846 1103 84-85 0.240272803833534 1103 86-87 0.3722963459029671 1103 88-89 0.3908976361125944 1103 90-91 0.3278237181783723 1103 92-93 0.44173783007036604 1103 94-95 0.4619256773983267 1103 96-97 0.2576051463860871 1103 98-99 0.35419753949696897 1104 1 0.13771607828005372 1104 2 0.13040457678101092 1104 3 0.11227804990085843 1104 4 0.16463895978603205 1104 5 0.15205275776048666 1104 6 0.11257181548916151 1104 7 0.005708904098064238 1104 8 0.05159627369399544 1104 9 0.05898890564694881 1104 10-11 0.0739047593994897 1104 12-13 0.09783057688035512 1104 14-15 0.15730994220940175 1104 16-17 0.19689268658736125 1104 18-19 0.08323336615492138 1104 20-21 0.1348776272672083 1104 22-23 0.14043987542120107 1104 24-25 0.11386288690853519 1104 26-27 0.16164310002670135 1104 28-29 0.09746040594735206 1104 30-31 0.1345243032736292 1104 32-33 0.14485153207623824 1104 34-35 0.1628290235830221 1104 36-37 0.2025100701068041 1104 38-39 0.11322824396457065 1104 40-41 0.06578152851427888 1104 42-43 -0.018489771157447876 1104 44-45 0.046080863328548105 1104 46-47 -0.010564865276016633 1104 48-49 0.047943698217778774 1104 50-51 0.21139093514944562 1104 52-53 0.038962320629934766 1104 54-55 0.14866257758043844 1104 56-57 0.04213422244254872 1104 58-59 0.12957351784764626 1104 60-61 0.09167581116888357 1104 62-63 0.064356122720163 1104 64-65 0.12349202178357643 1104 66-67 0.16193811036589523 1104 68-69 0.02197840282715191 1104 70-71 0.05115315066549897 1104 72-73 0.1033360512712207 1104 74-75 0.0047982563729505046 1104 76-77 -0.11397662388509744 1104 78-79 0.03637351776291453 1104 80-81 0.04749468608283536 1104 82-83 0.10684950417588723 1104 84-85 0.04795663695318808 1104 86-87 0.0461804524574454 1104 88-89 0.11628850176137462 1104 90-91 0.17378907446691017 1104 92-93 0.20017814154186198 1104 94-95 0.16950091360124375 1104 96-97 -0.0639843646687801 1104 98-99 0.07596271115224695 1105 1 0.08392264557457452 1105 2 0.0987596731340119 1105 3 0.1259266446988363 1105 4 0.11079979349270275 1105 5 0.115689969886418 1105 6 0.1524093821214194 1105 7 0.060770175743400046 1105 8 0.020254542748709525 1105 9 0.06853024280453468 1105 10-11 0.13312198460839397 1105 12-13 0.013426876720629366 1105 14-15 0.055135669239540164 1105 16-17 0.05537823526056229 1105 18-19 0.048529516498710734 1105 20-21 0.06506910978212233 1105 22-23 0.06802747532900355 1105 24-25 0.11899677634932004 1105 26-27 0.1139377736666276 1105 28-29 0.03570733039400409 1105 30-31 0.04221305475885373 1105 32-33 0.04278183360138854 1105 34-35 0.048494130762748 1105 36-37 0.03374105840679675 1105 38-39 -0.005729099218314104 1105 40-41 -0.011910564728673023 1105 42-43 -0.03824884199053713 1105 44-45 -0.0016962489382805757 1105 46-47 -0.054462089283752846 1105 48-49 -0.08216105482895841 1105 50-51 0.035120174454192465 1105 52-53 0.00990447920658255 1105 54-55 0.13356671965566136 1105 56-57 0.06249204008606313 1105 58-59 -0.017267415296096544 1105 60-61 -0.008714466365418616 1105 62-63 0.058964997065203306 1105 64-65 -0.040600943603365636 1105 66-67 0.07976609364047249 1105 68-69 -0.042668194626372724 1105 70-71 -0.10405045510528765 1105 72-73 -0.04962581738613636 1105 74-75 -0.02087782565046581 1105 76-77 -0.14320441408033346 1105 78-79 -0.003137086180156956 1105 80-81 -0.03468128718634844 1105 82-83 0.002323776090676688 1105 84-85 -0.10035716525695904 1105 86-87 -0.037917406869770076 1105 88-89 -0.07877752585245901 1105 90-91 -0.06742871723790245 1105 92-93 -0.027722583296956316 1105 94-95 -0.0470273653387423 1105 96-97 -0.10345334374251891 1105 98-99 0.02960915318461943 1106 1 0.11091719639775022 1106 2 0.1427810856120324 1106 3 0.14901920312084727 1106 4 0.13976451075397733 1106 5 0.0774557016920312 1106 6 0.03175844448576726 1106 7 0.13026874554356027 1106 8 0.07457301322249776 1106 9 0.07559326308066261 1106 10-11 0.025622036043131402 1106 12-13 0.07972009544690195 1106 14-15 0.1088615702976341 1106 16-17 0.08813515531386429 1106 18-19 0.11824203616170337 1106 20-21 0.04724283548058139 1106 22-23 0.08680397207204749 1106 24-25 0.07338439120975693 1106 26-27 0.1698811598031469 1106 28-29 0.021899640445120383 1106 30-31 0.044359821979519154 1106 32-33 0.1358950636841243 1106 34-35 0.023746566374313716 1106 36-37 0.010155335072610683 1106 38-39 -0.00978197012481985 1106 40-41 0.14828456806875323 1106 42-43 0.045842945970491655 1106 44-45 0.026977204391641862 1106 46-47 -0.009764889123935916 1106 48-49 -0.05207124192262569 1106 50-51 -0.04002156162450632 1106 52-53 0.0032317985363690127 1106 54-55 0.01892443923252074 1106 56-57 0.06853809545779654 1106 58-59 0.08256513518219322 1106 60-61 0.0194099800240366 1106 62-63 0.05563037694726347 1106 64-65 -0.05420965276888978 1106 66-67 -0.022423463552755152 1106 68-69 0.01596277439467997 1106 70-71 0.01159032672158844 1106 72-73 0.10540534617602049 1106 74-75 0.037126297216843795 1106 76-77 0.07145035497218899 1106 78-79 0.03371598750485916 1106 80-81 -0.06752447821638441 1106 82-83 0.0011237908771803973 1106 84-85 -0.08884790629726069 1106 86-87 -0.14100942777912806 1106 88-89 0.06368555931781117 1106 90-91 -0.055349573152785325 1106 92-93 -0.008166992592144595 1106 94-95 -0.09887245800136668 1106 96-97 -0.22210258662316917 1106 98-99 0.10229015418944343 1107 1 -0.02079903901715241 1107 2 0.06789245868208837 1107 3 0.02883474263579089 1107 4 0.03596696365184471 1107 5 0.04455256556818199 1107 6 -0.03463880031493716 1107 7 -0.09620622977261206 1107 8 -0.016157652270536005 1107 9 -0.1308593473000812 1107 10-11 -0.05074817125852604 1107 12-13 -0.14026288548639343 1107 14-15 -0.10494358988571406 1107 16-17 -0.11492793859172679 1107 18-19 -0.1594129570815781 1107 20-21 -0.10914758836256766 1107 22-23 -0.08597718087167294 1107 24-25 -0.0013960335597928974 1107 26-27 -0.04799082605763516 1107 28-29 -0.05057618746659642 1107 30-31 -0.12140470438510675 1107 32-33 -0.17169367904076438 1107 34-35 -0.19372403497023072 1107 36-37 -0.16361121970820136 1107 38-39 -0.14629955203403 1107 40-41 -0.2836147879830051 1107 42-43 -0.2524384692917323 1107 44-45 -0.060096047437319555 1107 46-47 -0.25117619653466505 1107 48-49 -0.19896739711858658 1107 50-51 -0.20840282504050123 1107 52-53 -0.21086001381262776 1107 54-55 -0.11612654503426256 1107 56-57 -0.16337276087714514 1107 58-59 -0.09518309681227777 1107 60-61 -0.005923182841705454 1107 62-63 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2215 26-27 -0.09809916565744459 2215 28-29 -0.05506509377725166 2215 30-31 -0.1495969843930638 2215 32-33 -0.1652400416815496 2215 34-35 -0.20255783475568023 2215 36-37 -0.1179543774350762 2215 38-39 -0.0926378923371729 2215 40-41 -0.06496068692595713 2215 42-43 0.01913645254988694 2215 44-45 -0.020826061429978893 2215 46-47 -0.014914388250559796 2215 48-49 0.06727880570681322 2215 50-51 -0.02869964878451725 2215 52-53 -0.029098990864049767 2215 54-55 0.0417886463220043 2215 56-57 0.0828431639953422 2215 58-59 -0.09302002944193788 2215 60-61 -0.11411184490007997 2215 62-63 -0.04949030818401923 2215 64-65 -0.12658611031726252 2215 66-67 -0.21019310828223325 2215 68-69 0.04009687382409055 2215 70-71 -0.09155797301599478 2215 72-73 -0.09189585824005775 2215 74-75 0.10416055390834345 2215 76-77 -0.03792293666904456 2215 78-79 0.07523087718770327 2215 80-81 0.18843323477153717 2215 82-83 0.11112250398375778 2215 84-85 0.1275270778782982 2215 86-87 0.10778131936246282 2215 88-89 -0.05792030251451408 2215 90-91 0.12643367295503793 2215 92-93 0.19370839147990537 2215 94-95 0.1267781227948639 2215 96-97 0.15151123315563808 2215 98-99 0.08952924396688289 2216 1 -0.07841339249433688 2216 2 -0.038701082677455645 2216 3 -0.011361361148402693 2216 4 -0.01938706114891886 2216 5 -0.16148174496757406 2216 6 -0.019936863462653776 2216 7 0.024561172194125902 2216 8 0.019195124243225337 2216 9 0.0344552188329601 2216 10-11 -0.07790403260154477 2216 12-13 -0.052391808287467256 2216 14-15 -0.09714688920293213 2216 16-17 -0.06442075625162147 2216 18-19 -0.1527994904667267 2216 20-21 -0.12837620485119317 2216 22-23 -0.2220490110225839 2216 24-25 -0.12532894760076374 2216 26-27 -0.2576170429950153 2216 28-29 -0.04130789315873784 2216 30-31 -0.08965327532002476 2216 32-33 -0.15615445631156888 2216 34-35 -0.19717217798114461 2216 36-37 -0.20333763797569304 2216 38-39 -0.07588602715993886 2216 40-41 -0.16600234813680714 2216 42-43 -0.15764138474317235 2216 44-45 -0.15524718439973384 2216 46-47 0.07826174208064174 2216 48-49 0.11305611380606706 2216 50-51 0.08822324979905005 2216 52-53 0.16799300503888048 2216 54-55 0.08571063275714152 2216 56-57 0.19489479559140221 2216 58-59 0.03311749806134401 2216 60-61 0.062359032233061384 2216 62-63 0.09507891420280146 2216 64-65 0.16596940897544954 2216 66-67 0.06704074374385982 2216 68-69 -0.08706356369454937 2216 70-71 0.06007521166645802 2216 72-73 -0.014001192209200042 2216 74-75 0.059764260865541985 2216 76-77 0.06336134673779625 2216 78-79 0.10548916288563603 2216 80-81 0.16744328796667318 2216 82-83 0.11677602081764249 2216 84-85 0.18762654711322924 2216 86-87 0.059527325968321776 2216 88-89 0.1789984048488833 2216 90-91 0.2530827070982937 2216 92-93 0.43954436707846867 2216 94-95 0.3751677529756563 2216 96-97 0.46483947188272623 2216 98-99 0.4488333355358982 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 6.0 7 210.0 8 1172.0 9 2143.0 10 3355.0 11 6044.0 12 10928.0 13 18636.0 14 26020.0 15 29808.0 16 32482.0 17 34587.0 18 37157.0 19 40021.0 20 43922.0 21 49410.0 22 55146.0 23 62948.0 24 72750.0 25 82576.0 26 93815.0 27 106580.0 28 126568.0 29 155290.0 30 192290.0 31 242925.0 32 315164.0 33 413247.0 34 558399.0 35 795092.0 36 1186912.0 37 1368133.0 38 379684.0 39 254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78819045406754 18.350994571728975 12.35660623028584 25.504208743917644 2 16.44216994917534 20.47357493664874 37.295363430390935 25.788891683784982 3 18.268668275957765 25.20954608332819 28.84049129631882 27.681294344395223 4 13.263099598176803 16.927570047040852 35.929020913939176 33.88030944084317 5 15.02449541343288 36.81269879887048 32.95251260988857 15.210293177808062 6 32.73239161975368 36.03304504472564 17.206022182645405 14.028541152875281 7 28.654498986349257 31.435123448998226 21.628522447786978 18.281855116865543 8 27.066492004338848 33.288837432916125 19.803248144696695 19.84142241804833 9 27.102144758433873 15.433348299441569 19.05661865184604 38.40788829027852 10-11 25.68559344490572 25.34557803460258 26.78561768205445 22.183210838437244 12-13 26.253256198276382 23.286046951605474 26.384604428643605 24.07609242147454 14-15 24.270012534242998 24.470649057394976 23.44066956880798 27.81866883955405 16-17 24.465613659849193 26.723970356713984 24.326464001721355 24.48395198171547 18-19 24.787153210872056 26.137266312472168 25.389979696421307 23.685600780234466 20-21 26.012607642523665 25.5354045525051 24.350927998899696 24.101059806071532 22-23 25.351774859199892 25.2668302241218 24.42746230939989 24.95393260727842 24-25 24.879053148454084 25.788370518555425 24.50476589280447 24.82781044018602 26-27 24.954049114476483 25.8492881251756 24.370981947978287 24.82568081236963 28-29 24.596296283277216 25.7641672460299 24.831215403456127 24.80832106723675 30-31 25.009111907983865 25.70762275096334 24.6454455360056 24.637819805047194 32-33 24.684865273223227 25.84676686376046 24.244218018651463 25.224149844364845 34-35 25.05707614709512 25.737466457090097 24.711039469362035 24.494417926452748 36-37 24.970643403079066 25.824590283684667 24.36893249877668 24.835833814459583 38-39 24.958566847701587 25.53287733051453 24.512624637569758 24.995931184214125 40-41 24.860919110579164 25.73639823744276 24.84699726789568 24.5556853840824 42-43 25.140456263560807 25.69056771471195 24.587334271236617 24.581641750490625 44-45 24.68430578907201 25.966888020399548 24.393475286207718 24.95533090432072 46-47 24.471650024130177 25.85364124190783 24.851467233850585 24.823241500111408 48-49 25.026951662139513 25.814148795486684 24.566628199404324 24.59227134296948 50-51 24.433002010796994 26.029016726689015 24.550703473308726 24.98727778920527 52-53 24.513262732831738 25.738782219285373 24.549710147540967 25.19824490034192 54-55 24.784188515503676 25.542386433064973 24.66629602880584 25.00712902262552 56-57 24.677696352232704 25.37132809488981 24.500173755599683 25.4508017972778 58-59 24.51342319314807 25.378449476547882 24.919632304421032 25.18849502588301 60-61 24.54109113626382 25.552548919765865 24.75698667140203 25.149373272568287 62-63 24.28919900349559 25.576954169782905 24.578982693820016 25.554864132901482 64-65 24.32166547416635 25.255223594573934 24.70256770126385 25.72054322999587 66-67 24.648519264333654 25.30853849206707 24.691201715000123 25.351740528599155 68-69 24.626754022281673 24.95303861408744 24.746793620831355 25.673413742799532 70-71 24.112669732630323 24.98396160933445 24.961306140862153 25.942062517173074 72-73 24.550741678145947 25.11551614582267 24.9143294730147 25.419412703016686 74-75 24.70822965814006 24.93181200652722 24.8970226817534 25.462935653579322 76-77 24.39301682816106 25.081857684230602 25.029952592381587 25.495172895226748 78-79 24.49547456062145 25.040497127454696 24.973516406838115 25.49051190508574 80-81 24.268033523674926 24.841778487131236 25.333635202487166 25.55655278670667 82-83 24.433024933699325 24.965057855877294 25.049070292927183 25.552846917496197 84-85 24.47130618059518 24.99543070146832 24.829805091146046 25.703458026790454 86-87 24.254577780005544 24.8403572671866 25.02759917440875 25.877465778399106 88-89 24.307460915687425 24.494909129030574 25.177530237600465 26.020099717681532 90-91 24.550222092359736 24.675396421031977 25.032061499396214 25.742319987212074 92-93 24.420233954197595 24.74598367828226 24.988874751401124 25.844907616119016 94-95 23.95009286831832 24.77281875594658 25.342766158583085 25.934322217152015 96-97 24.485334996822886 24.621336576363674 24.997531967515496 25.895796459297944 98-99 24.200999316286236 24.669169032564888 24.988217628200914 26.141614022947962 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1242.0 1 1144.0 2 1101.0 3 1090.5 4 968.5 5 854.0 6 748.5 7 721.0 8 707.5 9 663.0 10 623.5 11 610.5 12 635.5 13 652.5 14 721.0 15 849.5 16 949.0 17 1093.5 18 1422.5 19 1823.0 20 2406.0 21 3443.5 22 4951.0 23 6767.5 24 8956.0 25 12253.5 26 16507.0 27 20985.0 28 26574.5 29 34091.5 30 40580.0 31 47403.0 32 56450.0 33 64987.0 34 72769.0 35 82255.5 36 91282.5 37 101219.5 38 112946.0 39 117128.5 40 120259.5 41 129739.0 42 144923.5 43 164590.0 44 184698.0 45 205531.0 46 235156.0 47 259940.0 48 273991.5 49 284774.0 50 295367.5 51 300096.5 52 299568.5 53 301929.0 54 295598.5 55 285685.0 56 265625.5 57 248539.0 58 236172.0 59 209746.0 60 177298.0 61 140266.0 62 114270.0 63 93189.0 64 71414.0 65 55696.0 66 40780.0 67 30690.5 68 24155.0 69 20352.5 70 17646.5 71 14997.5 72 13989.0 73 12289.5 74 9266.0 75 7070.0 76 5199.0 77 3559.5 78 2336.5 79 1538.0 80 1075.5 81 765.0 82 534.5 83 383.0 84 281.5 85 216.0 86 149.5 87 91.5 88 65.0 89 56.5 90 42.5 91 27.0 92 20.0 93 16.5 94 13.5 95 12.0 96 10.0 97 6.0 98 2.5 99 9.5 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0023534179728391114 2 0.0 3 9.169160933139395E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.667664373255758E-4 22-23 0.0 24-25 0.00464570820612396 26-27 3.8204837221414147E-5 28-29 0.008725984821370991 30-31 5.959954606540608E-4 32-33 6.876870699854546E-5 34-35 5.34867721099798E-5 36-37 0.0 38-39 1.528193488856566E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.2158805588420204E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.528193488856566E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6543674.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.4043232503105 #Duplication Level Percentage of deduplicated Percentage of total 1 75.23765990397877 32.6563971106922 2 13.271463379581233 11.520777730640038 3 4.355979314607013 5.672050027286062 4 2.022627174517585 3.511630547904937 5 1.1951579257895388 2.5937510473069874 6 0.7713110651081495 2.008694087789724 7 0.5042310249651474 1.5320064480295847 8 0.37010712659256223 1.2851399487893727 9 0.283414810668912 1.1071285250579104 >10 1.7034827628272606 13.777337902009613 >50 0.14997698323604325 4.558233830797215 >100 0.11342238079789543 9.909970080179734 >500 0.013781003362112462 4.10511624207649 >1k 0.0073144727337314756 5.576707991757238 >5k 7.067123413093723E-5 0.1850584796829913 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.528193488856566E-5 0.0 2 0.0 0.0 0.0 1.528193488856566E-5 0.0 3 0.0 1.528193488856566E-5 0.0 1.528193488856566E-5 0.0 4 0.0 1.528193488856566E-5 0.0 3.056386977713132E-5 0.0 5 0.0 1.6810128377422226E-4 0.0 3.056386977713132E-5 0.0 6 0.0 1.6810128377422226E-4 0.0 3.056386977713132E-5 0.0 7 0.0 1.6810128377422226E-4 0.0 3.056386977713132E-5 0.0 8 0.0 1.6810128377422226E-4 0.0 3.056386977713132E-5 0.0 9 0.0 1.833832186627879E-4 0.0 3.056386977713132E-5 0.0 10-11 0.0 1.833832186627879E-4 0.0 3.056386977713132E-5 0.0 12-13 7.64096744428283E-6 2.903567628827475E-4 0.0 3.8204837221414147E-5 4.584580466569698E-5 14-15 3.8204837221414147E-5 3.285616001041617E-4 0.0 4.5845804665696974E-5 6.876870699854546E-5 16-17 6.876870699854546E-5 3.362025675484445E-4 0.0 4.5845804665696974E-5 9.169160933139395E-5 18-19 7.640967444282829E-5 3.7440740476985863E-4 0.0 6.876870699854546E-5 9.169160933139395E-5 20-21 7.640967444282829E-5 4.8138094898981826E-4 0.0 2.1394708843991924E-4 1.2225547910852527E-4 22-23 8.405064188711113E-5 5.348677210997981E-4 0.0 9.704028654239194E-4 1.5281934888565659E-4 24-25 9.169160933139395E-5 8.252244839825456E-4 0.0 0.0027049024752761218 1.5281934888565659E-4 26-27 9.169160933139395E-5 9.092751258696567E-4 0.0 0.005119448187669496 1.6810128377422226E-4 28-29 9.169160933139395E-5 9.627618979796365E-4 0.0 0.017153971912414953 1.6810128377422226E-4 30-31 9.169160933139395E-5 0.0010238896375338992 0.0 0.04775604652676768 1.6810128377422226E-4 32-33 9.169160933139395E-5 0.0010238896375338992 0.0 0.09987508546422086 1.757422512185051E-4 34-35 9.169160933139395E-5 0.001069735442199596 0.0 0.1676733895973424 1.9866515355135357E-4 36-37 9.169160933139395E-5 0.0011843499538638385 0.0 0.2693975891830797 1.9866515355135357E-4 38-39 9.169160933139395E-5 0.001253118660862384 0.0 0.41432381869879215 1.9866515355135357E-4 40-41 1.0697354421995962E-4 0.0012836825306395154 0.0 0.582982893096447 2.063061209956364E-4 42-43 1.0697354421995962E-4 0.0013142464004166467 0.0 0.7457354996596712 2.2922902332848488E-4 44-45 1.0697354421995962E-4 0.0013218873678609295 0.0 0.9373327583250632 2.521519256613334E-4 46-47 1.2225547910852527E-4 0.0013295283353052124 0.0 1.1517230228767508 2.597928931056162E-4 48-49 1.2225547910852527E-4 0.0013524512376380609 0.0 1.371316786257995 2.597928931056162E-4 50-51 1.2225547910852527E-4 0.0013753741399709094 0.0 1.6048018284529455 2.903567628827475E-4 52-53 1.2225547910852527E-4 0.0013982970423037579 0.0 1.839883832843751 2.9799773032703034E-4 54-55 1.2225547910852527E-4 0.0015358344563008487 0.0 2.081093893124871 3.0563869777131317E-4 56-57 1.2225547910852527E-4 0.0015740392935222629 0.0 2.358346396840674 3.0563869777131317E-4 58-59 1.2225547910852527E-4 0.001703935740075071 0.0 2.6556640810651633 3.0563869777131317E-4 60-61 1.2225547910852527E-4 0.0017650634796293335 0.0 2.9572607070584507 3.0563869777131317E-4 62-63 1.2225547910852527E-4 0.0017727044470736164 0.0 3.2667810162914597 3.0563869777131317E-4 64-65 1.2225547910852527E-4 0.0018949599261821417 0.0 3.5899251704776245 3.13279665215596E-4 66-67 1.2225547910852527E-4 0.00197136960062497 0.0 3.9442291899015753 3.285616001041617E-4 68-69 1.2225547910852527E-4 0.0019942925029578186 0.0 4.323045432886785 3.362025675484445E-4 70-71 1.2225547910852527E-4 0.00202485637273495 0.0 4.739822002135192 3.362025675484445E-4 72-73 1.2225547910852527E-4 0.0020477792750677984 0.0 5.15317541796856 3.896893396584243E-4 74-75 1.2225547910852527E-4 0.0020554202425120812 0.0 5.575881377953731 3.9733030710270713E-4 76-77 1.2225547910852527E-4 0.002063061209956364 0.0 6.0297854080139075 4.2025320943555564E-4 78-79 1.2225547910852527E-4 0.002070702177400647 0.0 6.506123318490499 4.278941768798385E-4 80-81 1.2225547910852527E-4 0.002116547982066344 0.0 7.018648850783214 4.3553514432412125E-4 82-83 1.2225547910852527E-4 0.0021241889495106268 0.0 7.552362785798926 4.5845804665696976E-4 84-85 1.2225547910852527E-4 0.0021700347541763238 0.0 8.084365144107117 4.7373998154553543E-4 86-87 1.2225547910852527E-4 0.0022158805588420208 0.0 8.639771785697148 4.890219164341011E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18840 0.0 22.978838 1 GTACATG 30270 0.0 19.079565 1 AACGCAG 21390 0.0 18.825787 6 TACATGG 29845 0.0 18.587238 2 ATCAACG 21635 0.0 18.333195 3 CAACGCA 21805 0.0 18.211586 5 TATTCGC 440 0.0 17.965622 9 GTACTAG 1845 0.0 17.642532 1 TATCAAC 24480 0.0 17.55119 2 GTATAAG 2030 0.0 17.409119 1 TCAACGC 22900 0.0 17.34077 4 ACGCAGA 22735 0.0 17.139378 7 CGCAGAG 22765 0.0 16.994238 8 ACATGGG 30185 0.0 16.929827 3 GTACAAG 3025 0.0 16.755615 1 CATGGGG 14430 0.0 16.079784 4 GCAGAGT 23710 0.0 15.826613 9 GTATTAC 1200 0.0 15.500225 1 CTAACGC 990 0.0 15.499753 3 TAGTACT 2385 0.0 15.402268 4 >>END_MODULE