FastQCFastQC Report
Fri 27 May 2016
ERR765375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765375_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6543674
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159600.24389968082150792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99390.15188715085745408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88960.1359480927686801No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG5700.035.0799832
GTATCAA254800.034.676371
ATCAACG321600.027.2270163
GGTATCA160000.026.927691
CAACGCA326350.026.7879835
TCAACGC340150.025.7832094
AACGCAG345250.025.6299446
TATCAAC350600.025.2401922
ACGCAGA379900.023.0352447
CGCAGAG384750.022.756968
TAACGCA4600.022.2397864
GCAGAGT410350.021.179099
AGTACTT230250.020.81360612-13
AGAGTAC390850.019.91732410-11
CTAACGC8300.018.4884973
GAGTACT217800.018.2987712-13
ATCACGC10950.018.2608133
TACTTTT266950.018.05669814-15
TCACGCA11400.017.9478994
GTACTAG17450.017.5977271
Produced by FastQC (version 0.11.5)