##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765375_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6543674 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.380060650943186 33.0 33.0 33.0 33.0 33.0 2 32.279295239952354 33.0 33.0 33.0 33.0 33.0 3 32.32964371391362 33.0 33.0 33.0 33.0 33.0 4 36.23895933691073 37.0 37.0 37.0 33.0 37.0 5 36.17469574431733 37.0 37.0 37.0 33.0 37.0 6 36.19355258223438 37.0 37.0 37.0 33.0 37.0 7 36.094575768902914 37.0 37.0 37.0 33.0 37.0 8 36.100590738475056 37.0 37.0 37.0 33.0 37.0 9 36.12783017613653 37.0 37.0 37.0 33.0 37.0 10-11 36.08310522498523 37.0 37.0 37.0 33.0 37.0 12-13 36.06019622921313 37.0 37.0 37.0 33.0 37.0 14-15 37.567484336780836 40.0 37.0 40.0 33.0 40.0 16-17 37.56468010172878 40.0 37.0 40.0 33.0 40.0 18-19 37.60377702189932 40.0 37.0 40.0 33.0 40.0 20-21 37.58871912017622 40.0 37.0 40.0 33.0 40.0 22-23 37.5556124128433 40.0 37.0 40.0 33.0 40.0 24-25 37.534782982770835 40.0 37.0 40.0 33.0 40.0 26-27 37.4177476598009 40.0 37.0 40.0 33.0 40.0 28-29 37.32250277137889 38.5 37.0 40.0 33.0 40.0 30-31 37.18900773479852 37.0 37.0 40.0 33.0 40.0 32-33 37.09296948472678 37.0 37.0 40.0 33.0 40.0 34-35 37.001905810099956 37.0 37.0 40.0 33.0 40.0 36-37 36.863932173271465 37.0 37.0 40.0 33.0 40.0 38-39 36.75894742005791 37.0 37.0 40.0 33.0 40.0 40-41 36.61499724772352 37.0 37.0 40.0 33.0 40.0 42-43 36.4788947310028 37.0 37.0 40.0 33.0 40.0 44-45 36.23126564679109 37.0 37.0 40.0 33.0 40.0 46-47 36.03368642753291 37.0 37.0 40.0 33.0 40.0 48-49 35.80529347886218 37.0 37.0 40.0 30.0 40.0 50-51 35.62598060355696 37.0 37.0 40.0 27.0 40.0 52-53 35.48139042378945 37.0 35.0 40.0 27.0 40.0 54-55 35.28711393629939 37.0 33.0 40.0 27.0 40.0 56-57 35.082986560760816 37.0 33.0 40.0 27.0 40.0 58-59 34.87398707515075 37.0 33.0 40.0 27.0 40.0 60-61 34.71391087025423 37.0 33.0 37.0 27.0 40.0 62-63 34.50325787928922 37.0 33.0 37.0 27.0 40.0 64-65 34.27666758765794 37.0 33.0 37.0 27.0 40.0 66-67 34.04377441480122 37.0 33.0 37.0 27.0 40.0 68-69 33.832049472513454 37.0 33.0 37.0 27.0 40.0 70-71 33.64246774824051 37.0 33.0 37.0 27.0 37.0 72-73 33.43824654162172 37.0 33.0 37.0 27.0 37.0 74-75 33.20664744301138 37.0 33.0 37.0 27.0 37.0 76-77 33.03387195022246 37.0 33.0 37.0 27.0 37.0 78-79 32.86985399945046 35.0 33.0 37.0 27.0 37.0 80-81 32.696514144806116 33.0 33.0 37.0 27.0 37.0 82-83 32.60624291491294 33.0 33.0 37.0 27.0 37.0 84-85 32.483986518888315 33.0 33.0 37.0 27.0 37.0 86-87 32.37578491838072 33.0 33.0 37.0 22.0 37.0 88-89 32.26619793406579 33.0 33.0 37.0 22.0 37.0 90-91 32.169634367482246 33.0 33.0 37.0 22.0 37.0 92-93 32.101335809210546 33.0 33.0 37.0 22.0 37.0 94-95 32.00115279275832 33.0 33.0 37.0 22.0 37.0 96-97 31.944382849756877 33.0 33.0 37.0 22.0 37.0 98-99 31.14745485487205 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03190526793422066 1101 2 0.17729736869570445 1101 3 0.13502751556899995 1101 4 0.09281090749921361 1101 5 0.07917078473313666 1101 6 0.11347272315088475 1101 7 0.08894623271643809 1101 8 0.10454536739172227 1101 9 0.0477439714567538 1101 10-11 0.11727403750940368 1101 12-13 0.1138625119354586 1101 14-15 0.1550967786010986 1101 16-17 0.18504884649321696 1101 18-19 0.1638803275388767 1101 20-21 0.20294643742830942 1101 22-23 0.14183271231753736 1101 24-25 0.1738663391735642 1101 26-27 0.22040086600025433 1101 28-29 0.17130907407752716 1101 30-31 0.20632648448717106 1101 32-33 0.28204738189899103 1101 34-35 0.3088553434990189 1101 36-37 0.27518481351908974 1101 38-39 0.20244972446302967 1101 40-41 0.16255523807778616 1101 42-43 0.20979904601519905 1101 44-45 0.25005499191485825 1101 46-47 0.3205775550980974 1101 48-49 0.1811029904813779 1101 50-51 0.29264133838459827 1101 52-53 0.26653857594210706 1101 54-55 0.22318553584091916 1101 56-57 0.2879059003067681 1101 58-59 0.3009601149700103 1101 60-61 0.26462586873088156 1101 62-63 0.2404271333681578 1101 64-65 0.20526355712443234 1101 66-67 0.25375465528706087 1101 68-69 0.26340690996274674 1101 70-71 0.18208363562985141 1101 72-73 0.18127714700703024 1101 74-75 0.2279688383476568 1101 76-77 0.321828546929531 1101 78-79 0.24873038190205676 1101 80-81 0.27084493548392174 1101 82-83 0.2724840515411202 1101 84-85 0.25298730508441736 1101 86-87 0.253901518243282 1101 88-89 0.2265480960240538 1101 90-91 0.2854167533012628 1101 92-93 0.20613526311036878 1101 94-95 0.3408132127126393 1101 96-97 0.30421621582571134 1101 98-99 0.3519204500552533 1102 1 0.002876185719017599 1102 2 0.10195772642201462 1102 3 0.089020387438552 1102 4 0.039710330377900505 1102 5 0.08210744669682413 1102 6 0.11625137465052404 1102 7 0.05758181899327042 1102 8 0.09743180068507229 1102 9 0.06592426687821273 1102 10-11 0.08083094449000328 1102 12-13 0.07588472312790628 1102 14-15 0.1364382040284795 1102 16-17 0.11654140060365137 1102 18-19 0.18363002592227673 1102 20-21 0.14535476024663296 1102 22-23 0.16723940747277766 1102 24-25 0.19729630933290565 1102 26-27 0.14082193452289715 1102 28-29 0.14180501423814462 1102 30-31 0.1864528039948823 1102 32-33 0.15884847543055258 1102 34-35 0.19533436967979867 1102 36-37 0.18183871510426286 1102 38-39 0.22075028391860485 1102 40-41 0.16937326155170496 1102 42-43 0.15890435174596007 1102 44-45 0.21885361294212657 1102 46-47 0.22750533428802555 1102 48-49 0.19848461464299305 1102 50-51 0.20170441871667322 1102 52-53 0.23313700179461705 1102 54-55 0.07463050106267133 1102 56-57 0.11392155337071586 1102 58-59 0.177050166555901 1102 60-61 0.16758799976389582 1102 62-63 0.2279390921468334 1102 64-65 0.11129763558874828 1102 66-67 0.22296887273599708 1102 68-69 0.1400518854943229 1102 70-71 0.13382160057150116 1102 72-73 0.174246550450313 1102 74-75 0.3137422109627863 1102 76-77 0.27770248179367485 1102 78-79 0.2225181450196132 1102 80-81 0.28273858357022874 1102 82-83 0.21478247259457817 1102 84-85 0.1722979651760852 1102 86-87 0.17225955409514881 1102 88-89 0.3496225266973241 1102 90-91 0.19700376872683734 1102 92-93 0.30514868971091147 1102 94-95 0.27361300694419555 1102 96-97 0.3348221782929244 1102 98-99 0.28561315819992217 1103 1 0.016772563811748853 1103 2 0.12654711949367936 1103 3 0.09439469306054349 1103 4 0.12445425092631979 1103 5 0.06082493168959502 1103 6 0.08005885777746613 1103 7 0.07517612257958461 1103 8 0.13501520170125758 1103 9 0.09968325580657478 1103 10-11 0.10173566963896974 1103 12-13 0.081763091370604 1103 14-15 0.17320798527122605 1103 16-17 0.15197983458271835 1103 18-19 0.18966536706279413 1103 20-21 0.06643182974428896 1103 22-23 0.14499008767170096 1103 24-25 0.16035901633989624 1103 26-27 0.20086043279813737 1103 28-29 0.14185942933713136 1103 30-31 0.18720871973277298 1103 32-33 0.1922135862041756 1103 34-35 0.23518026375713674 1103 36-37 0.17555885222216006 1103 38-39 0.11214233863803003 1103 40-41 0.11875413502727383 1103 42-43 0.14582085010664514 1103 44-45 0.17783732584317136 1103 46-47 0.14535031593494807 1103 48-49 0.1225963339731635 1103 50-51 0.15292605805380788 1103 52-53 0.08017667785534144 1103 54-55 0.027956703411327055 1103 56-57 0.08279142263477013 1103 58-59 0.1702446644073774 1103 60-61 0.10941879862031811 1103 62-63 0.0710047349624574 1103 64-65 0.0820688971176935 1103 66-67 0.21187905723454747 1103 68-69 0.24106444418146822 1103 70-71 0.008080087931951141 1103 72-73 0.0966165691318821 1103 74-75 0.1744461761657945 1103 76-77 0.18317083878230989 1103 78-79 0.1696229012943533 1103 80-81 0.12541362698521397 1103 82-83 0.06188759136510669 1103 84-85 0.11894466321207631 1103 86-87 0.10752963728874931 1103 88-89 0.15542709446202707 1103 90-91 0.035630467258926046 1103 92-93 0.10958410271520336 1103 94-95 0.046541476871034604 1103 96-97 0.07839759201976193 1103 98-99 0.017056000616602063 1104 1 0.0021127200655044476 1104 2 -6.718559212401942E-4 1104 3 -0.013272027257066554 1104 4 -0.0359337749223414 1104 5 -0.06585790426245808 1104 6 -0.01162783549266777 1104 7 0.0739297555812044 1104 8 0.13613465645963174 1104 9 0.05535092334090308 1104 10-11 0.01012094263903407 1104 12-13 -0.0329184902593056 1104 14-15 0.007597687249472074 1104 16-17 0.036355291309526194 1104 18-19 0.06259929684664911 1104 20-21 0.07003430582798842 1104 22-23 0.03360998181283037 1104 24-25 0.039049970235232934 1104 26-27 -8.733253026989019E-4 1104 28-29 -0.03501488499452421 1104 30-31 -0.03464645601107463 1104 32-33 -0.005423922097847367 1104 34-35 -0.01776212067593974 1104 36-37 0.15895453165590823 1104 38-39 0.03697079436027906 1104 40-41 0.025500464099977194 1104 42-43 0.09177973128089434 1104 44-45 0.11645527745001516 1104 46-47 0.05154228272316175 1104 48-49 0.06600957021678511 1104 50-51 0.005260151750562159 1104 52-53 0.1491892784277411 1104 54-55 0.06499611465693533 1104 56-57 0.162971467812568 1104 58-59 0.10327163233336023 1104 60-61 0.09398221866468504 1104 62-63 0.020635930953062598 1104 64-65 -0.001164534452371413 1104 66-67 0.14185883637764363 1104 68-69 0.08465232350754093 1104 70-71 -0.00196077928935523 1104 72-73 0.0714046941422879 1104 74-75 0.11059174413506412 1104 76-77 0.13260796634760652 1104 78-79 0.05368903256156443 1104 80-81 0.11950018261291717 1104 82-83 0.18411129361345502 1104 84-85 0.14169488475349112 1104 86-87 0.17268769482846835 1104 88-89 0.15030287401953757 1104 90-91 0.1060254122297053 1104 92-93 0.11182606478583068 1104 94-95 0.0938396891844846 1104 96-97 0.07749644111444809 1104 98-99 0.15320101357607996 1105 1 -0.026717112842554513 1105 2 0.08296221531765013 1105 3 0.04223337149761619 1105 4 0.07960365357581622 1105 5 0.046654634937759454 1105 6 0.03949618815406808 1105 7 0.06496540424336672 1105 8 0.08592339731291077 1105 9 -0.028124541218204513 1105 10-11 0.007549118181344738 1105 12-13 -0.018219564978330993 1105 14-15 0.001210253151285201 1105 16-17 0.04477864108584839 1105 18-19 0.07725526826538953 1105 20-21 -0.014705781711043642 1105 22-23 -0.009062083202721283 1105 24-25 0.07084555732951259 1105 26-27 -0.005721988518438081 1105 28-29 -0.03152055685217192 1105 30-31 0.0334098299833272 1105 32-33 0.08223879330045492 1105 34-35 0.08788120928113585 1105 36-37 0.05244469832581444 1105 38-39 0.007471859177798024 1105 40-41 -0.0019973628444489577 1105 42-43 0.09106574759727692 1105 44-45 0.07523216977359226 1105 46-47 0.037232600188119136 1105 48-49 0.18012676182550535 1105 50-51 0.08046491810752343 1105 52-53 0.0012013432485105113 1105 54-55 0.028209783846044445 1105 56-57 -0.03910828434140967 1105 58-59 0.12663243625435427 1105 60-61 0.00441104916715318 1105 62-63 -0.010642860511509866 1105 64-65 0.05866880577954703 1105 66-67 0.04358389960118103 1105 68-69 0.0919453800370107 1105 70-71 0.001555035929087012 1105 72-73 0.03664213546370121 1105 74-75 0.03503899468208971 1105 76-77 0.04823227648869732 1105 78-79 0.03783093126430259 1105 80-81 0.043425149093891946 1105 82-83 0.06371964244831929 1105 84-85 -0.027767191673142122 1105 86-87 0.08935293105238884 1105 88-89 0.07886949531783216 1105 90-91 0.07187899900907979 1105 92-93 0.027748972240857483 1105 94-95 0.04138436972299786 1105 96-97 -0.007142521224125176 1105 98-99 0.1698856267194344 1106 1 -0.004568126664437955 1106 2 0.10978018989774085 1106 3 0.0404743034354027 1106 4 0.07089670445207474 1106 5 0.07920432403636113 1106 6 0.06649979895124858 1106 7 -0.01629398357801648 1106 8 0.02729584418671749 1106 9 0.008218450040878622 1106 10-11 -0.016762899981159762 1106 12-13 0.003928616305010735 1106 14-15 0.058606302562871804 1106 16-17 0.06685626156407665 1106 18-19 0.010955006709927773 1106 20-21 0.03433199506281426 1106 22-23 0.1008281819602388 1106 24-25 0.07268438359191975 1106 26-27 0.062127985026044996 1106 28-29 0.023498883712200325 1106 30-31 0.057059679872409674 1106 32-33 0.011446703625594523 1106 34-35 0.0956198012916758 1106 36-37 0.05829514463800223 1106 38-39 -0.031657766100892104 1106 40-41 0.0058703411575038444 1106 42-43 0.0616059611605948 1106 44-45 0.051171951159460605 1106 46-47 0.09885425386757163 1106 48-49 0.03714034782093023 1106 50-51 0.07104399787958471 1106 52-53 0.09542718819955098 1106 54-55 0.029650462041736603 1106 56-57 0.08649079785082847 1106 58-59 0.09959996212721478 1106 60-61 -0.03244434104425409 1106 62-63 0.030939485248659082 1106 64-65 0.07764923046401861 1106 66-67 0.04760737712506824 1106 68-69 0.09123781373567397 1106 70-71 -0.018234965652432322 1106 72-73 0.05513720369678765 1106 74-75 0.023775724323201075 1106 76-77 0.12383889818909921 1106 78-79 0.004456936676028533 1106 80-81 0.020901901573594728 1106 82-83 -0.054835520994785725 1106 84-85 -0.04477199724826164 1106 86-87 0.058620748320706184 1106 88-89 0.07162925586438718 1106 90-91 -0.03550249260461413 1106 92-93 0.018048944937248734 1106 94-95 0.048682551783748806 1106 96-97 0.06705406717640727 1106 98-99 0.05048873966760681 1107 1 -0.009649049341675209 1107 2 0.06348013673851227 1107 3 0.045932286636386266 1107 4 0.009464143011051362 1107 5 0.012416977651596994 1107 6 0.0014198751226359718 1107 7 0.0126142217508729 1107 8 0.03792928326212319 1107 9 0.07295823403647717 1107 10-11 -0.04270857729953548 1107 12-13 -0.06430314342958354 1107 14-15 0.02141781123098241 1107 16-17 -0.012417074133395545 1107 18-19 -0.047256224463730234 1107 20-21 0.029014478571433244 1107 22-23 0.015121531821264966 1107 24-25 -0.001087469723394463 1107 26-27 0.011783261501832953 1107 28-29 -0.08739292774806984 1107 30-31 -0.0509668270969712 1107 32-33 0.029697348747532715 1107 34-35 -0.018493949018768774 1107 36-37 0.07526811361119456 1107 38-39 0.008828949453977941 1107 40-41 0.015464881068410818 1107 42-43 -0.04051256874676312 1107 44-45 -0.013518185036538455 1107 46-47 -0.014270800530553629 1107 48-49 -0.04853589249741219 1107 50-51 0.002320208599542184 1107 52-53 -0.036063731503361396 1107 54-55 -0.007865234323347181 1107 56-57 -0.022076120997745363 1107 58-59 0.02375029599284062 1107 60-61 0.009397388977014032 1107 62-63 -0.061435822170182064 1107 64-65 -0.09188743708526204 1107 66-67 -0.046806760002546355 1107 68-69 -0.08804858234218926 1107 70-71 -0.02965863502232935 1107 72-73 -0.06137856985206014 1107 74-75 0.05337414950408004 1107 76-77 0.0017477235962530813 1107 78-79 0.01300891143548455 1107 80-81 -0.10561515772719332 1107 82-83 -0.08445063873286074 1107 84-85 -0.0188056039640756 1107 86-87 -0.015113884842477887 1107 88-89 -0.026193655037438646 1107 90-91 -0.00294802130321159 1107 92-93 0.06629126599868584 1107 94-95 0.0067704869772171605 1107 96-97 -0.02162656295603682 1107 98-99 -0.0610284051245813 1108 1 0.00805463367549919 1108 2 0.02256420176641427 1108 3 0.04936749367356441 1108 4 -0.02776928737951323 1108 5 0.04284794099001488 1108 6 -0.0020750394822570684 1108 7 -0.07693961178822661 1108 8 -0.08074648452119249 1108 9 -0.019425320543923874 1108 10-11 -0.09016540512095617 1108 12-13 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-0.08036648223015419 2215 4 -0.1202209615777079 2215 5 -0.06711343744347431 2215 6 -0.11651284186348221 2215 7 -0.1203402183013722 2215 8 -0.15512367494956436 2215 9 -0.04448174750672962 2215 10-11 -0.12272720766063117 2215 12-13 -0.05694281282746516 2215 14-15 -0.13679728482187414 2215 16-17 -0.2034243449283295 2215 18-19 -0.16618228648381006 2215 20-21 -0.19222892445640127 2215 22-23 -0.10590116000916794 2215 24-25 -0.2292236452957397 2215 26-27 -0.27042724783712657 2215 28-29 -0.19219564281944201 2215 30-31 -0.12794305295538067 2215 32-33 -0.16309553656635956 2215 34-35 -0.11348191359577697 2215 36-37 -0.17123026807507813 2215 38-39 -0.07881472210852536 2215 40-41 -0.1048083835136282 2215 42-43 -0.07824456456577877 2215 44-45 -0.11345190116256276 2215 46-47 -0.1661405717029254 2215 48-49 -0.08777328802933937 2215 50-51 -0.14491550434173917 2215 52-53 -0.13989223092939085 2215 54-55 -0.10090944147256664 2215 56-57 -0.10970158528770924 2215 58-59 -0.14910577958595894 2215 60-61 -0.1318479866560196 2215 62-63 -0.1661481427440208 2215 64-65 -0.0555617044595067 2215 66-67 -0.13728329874760448 2215 68-69 -0.08743067416072847 2215 70-71 -0.1569483622694392 2215 72-73 -0.18763867312544136 2215 74-75 -0.2260254426313253 2215 76-77 -0.21551578120065074 2215 78-79 -0.18758664899141309 2215 80-81 -0.18086669201084504 2215 82-83 -0.10169490565689898 2215 84-85 -0.0942546790183556 2215 86-87 -0.09696179463132637 2215 88-89 -0.033501415982286176 2215 90-91 -0.13399341888621308 2215 92-93 -0.03821550405105256 2215 94-95 0.0699777748765058 2215 96-97 -0.06312728338182794 2215 98-99 -0.08522457754726886 2216 1 -0.05374100952307259 2216 2 -0.020421948821216063 2216 3 -0.06134598923381418 2216 4 -0.12284645113236081 2216 5 -0.06235721958984186 2216 6 -0.1044415511697423 2216 7 -0.05237698194581242 2216 8 -0.1437746249894829 2216 9 -0.06819190640018746 2216 10-11 -0.09319167488867208 2216 12-13 -0.04907105715891902 2216 14-15 -0.2646195946007168 2216 16-17 -0.20844108279496965 2216 18-19 -0.1476335639170614 2216 20-21 -0.1988675895140375 2216 22-23 -0.26276476588834896 2216 24-25 -0.2905192694665999 2216 26-27 -0.30883079577017014 2216 28-29 -0.20296617155771912 2216 30-31 -0.26815648861570907 2216 32-33 -0.2362547904911665 2216 34-35 -0.23327144931177202 2216 36-37 -0.21243798633084054 2216 38-39 -0.20349313348821596 2216 40-41 -0.20825009117867666 2216 42-43 -0.2625313477520166 2216 44-45 -0.24220043226521426 2216 46-47 -0.24189871422657916 2216 48-49 -0.14427985621012596 2216 50-51 -0.13412573947749706 2216 52-53 -0.15206273410100835 2216 54-55 -0.1259452821299405 2216 56-57 -0.2233257009938754 2216 58-59 -0.331872846862467 2216 60-61 -0.28190260287932034 2216 62-63 -0.17654139227398957 2216 64-65 -0.20532775814047 2216 66-67 -0.21828041277521493 2216 68-69 -0.11456085267908378 2216 70-71 -0.1961932439109617 2216 72-73 -0.19182741493674627 2216 74-75 -0.15294025419387225 2216 76-77 -0.16956663670969618 2216 78-79 -0.07854636124903891 2216 80-81 -0.2566419351489415 2216 82-83 -0.21485778805121925 2216 84-85 -0.15327697063584367 2216 86-87 -0.2873079368425735 2216 88-89 -0.1982859769064973 2216 90-91 -0.21753638069380443 2216 92-93 -0.037243507604216575 2216 94-95 -0.16208464712465798 2216 96-97 -0.23305299120806566 2216 98-99 -0.22996238555680293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 19.0 10 57.0 11 117.0 12 225.0 13 462.0 14 954.0 15 2514.0 16 5490.0 17 10284.0 18 16695.0 19 23295.0 20 30036.0 21 37741.0 22 46760.0 23 48158.0 24 44699.0 25 45270.0 26 53778.0 27 69282.0 28 91819.0 29 121617.0 30 160316.0 31 211743.0 32 278780.0 33 369252.0 34 492367.0 35 705308.0 36 1180645.0 37 1825241.0 38 669985.0 39 760.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92215603288872 18.484606940710794 12.879371583425241 25.71386544297524 2 16.493318585247373 20.71330264924567 36.71908166574313 26.074297099763832 3 18.371865713359192 25.02956290304193 29.462088117470397 27.136483266128476 4 13.520004205588481 16.872004931786027 35.95374708458887 33.654243778036616 5 14.738795563049539 36.514596635379306 33.12868706007636 15.617920741494803 6 32.53999205950663 36.211751991312525 16.98301290681657 14.265243042364272 7 28.056180671592134 31.332138489784178 22.06038992773784 18.551290910885843 8 27.416295493938115 32.29231774076765 20.07954552748196 20.211841237812276 9 27.325459672960477 14.080087119254411 19.699529652608 38.89492355517711 10-11 25.814832768258324 24.571418136050177 27.27063573154775 22.34311336414375 12-13 26.72104386618282 22.51393483232814 27.195975838649666 23.569045462839377 14-15 24.216578683610884 23.469312981812198 25.003715468398497 27.310392866178418 16-17 23.905806313674677 25.80971100741155 25.71861878174827 24.565863897165503 18-19 24.175850289663693 24.65770109311004 27.884636691325284 23.28181192590099 20-21 25.16364689985516 24.16545964629591 27.070690692764998 23.60020276108393 22-23 24.495230049663235 23.840834674832518 27.227617084836435 24.436318190667812 24-25 24.25414224486122 24.315980594387803 27.239368892765746 24.190508267985233 26-27 24.363385156412132 24.414594920223713 27.079137805459137 24.142882117905017 28-29 23.963411074573703 24.32468365630684 27.538673228525752 24.17323204059371 30-31 24.433241575319993 24.206365519240684 27.26641095863615 24.093981946803172 32-33 24.200074759225476 24.225106568572947 26.89549479390324 24.679323878298337 34-35 24.570761012849967 24.13611986171683 27.269359689984558 24.02375943544865 36-37 24.58468285553345 24.28284935954939 26.80095310371513 24.33151468120203 38-39 24.601240831985212 23.92567233636639 26.932477076333573 24.540609755314826 40-41 24.512483354152423 24.148788585739446 27.10883060494762 24.22989745516051 42-43 24.909691405776023 24.171612155495524 26.662216057829287 24.25648038089917 44-45 24.513407911213182 24.451057616867832 26.24476708344578 24.790767388473203 46-47 24.434560768155627 24.392963341388953 26.37708571667843 24.795390173776994 48-49 25.106736674229186 24.602585642255406 25.711893654848943 24.578784028666465 50-51 24.504804181870917 24.89842861976315 25.605118775782536 24.9916484225834 52-53 24.521790052499558 24.76179283992448 25.524766362138458 25.1916507454375 54-55 24.748505197538876 24.671400195058617 25.58467918786908 24.995415419533433 56-57 24.600981039092108 24.509136610411826 25.42832207105672 25.46156027943935 58-59 24.386407391321757 24.474072210810014 25.91577758916474 25.22374280870349 60-61 24.411935863553104 24.632293723678778 25.802973986784792 25.152796425983325 62-63 24.216625094709794 24.61212156962587 25.6440571458786 25.52719618978574 64-65 24.254019989382112 24.321497372882572 25.698980419868107 25.72550221786721 66-67 24.499906321739136 24.50245076389808 25.71462148022655 25.283021434136238 68-69 24.529499788650842 24.169350429132013 25.688535217371772 25.612614564845376 70-71 23.996305439421338 24.215433103788484 25.86226789415243 25.92599356263775 72-73 24.400115286916797 24.352557905543584 25.74651487833899 25.50081192920063 74-75 24.525423114945724 24.213579808642578 25.67266905686426 25.588328019547436 76-77 24.24780881602215 24.355027010472952 25.69729458206566 25.699869591439235 78-79 24.283798476130716 24.461084270504717 25.585667006664377 25.669450246700187 80-81 23.971391907878036 24.268732560444263 25.84248937867158 25.917386153006117 82-83 24.07171598644095 24.405381778293187 25.558151701792582 25.964750533473286 84-85 24.106558661614336 24.418983946942717 25.326765535627736 26.147691855815214 86-87 23.835963634752073 24.244965699361668 25.50463734849724 26.414433317389026 88-89 23.802503556118204 23.999068104477438 25.640651428285 26.55777691111936 90-91 23.99193899628981 24.09192979646378 25.497753378032467 26.41837782921394 92-93 23.81827866536962 24.166788223479212 25.412513135967863 26.602419975183306 94-95 23.28821092097982 24.188815129025933 25.830600768047347 26.6923731819469 96-97 23.7450699558894 24.056483390060933 25.536467044198623 26.661979609851038 98-99 23.39359040777061 24.04001295916792 25.574329538803386 26.992067094258083 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 159.0 1 179.5 2 529.0 3 843.5 4 595.5 5 528.0 6 1057.5 7 2725.5 8 4852.5 9 4705.5 10 2836.5 11 1825.5 12 1807.0 13 2000.0 14 2297.5 15 2695.0 16 3242.5 17 3774.5 18 4336.0 19 5177.5 20 6205.0 21 7615.5 22 9546.5 23 11549.0 24 13885.0 25 17316.5 26 21480.5 27 25759.5 28 30947.0 29 37863.5 30 43490.5 31 49529.0 32 57437.5 33 64757.5 34 71662.0 35 79826.0 36 88001.0 37 97717.5 38 108522.0 39 112331.5 40 115481.5 41 123909.0 42 137848.5 43 158211.5 44 178678.0 45 198909.5 46 230379.5 47 256270.0 48 270091.0 49 281199.0 50 293192.5 51 299584.5 52 300908.5 53 303673.0 54 295387.0 55 285454.0 56 266307.0 57 249794.5 58 238840.0 59 212616.5 60 177333.5 61 138169.5 62 113552.0 63 92449.0 64 70084.5 65 55002.5 66 39999.0 67 29671.0 68 23016.5 69 19330.0 70 16978.0 71 14804.5 72 14730.0 73 12606.5 74 8269.0 75 6315.5 76 4875.0 77 3157.0 78 1776.5 79 1125.5 80 764.5 81 524.5 82 324.5 83 215.0 84 144.0 85 93.5 86 47.5 87 18.5 88 13.0 89 10.0 90 4.0 91 2.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05625280232481018 2 0.0 3 0.0 4 0.0 5 0.0053639591458865465 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012913234980837982 16-17 0.004668631108456808 18-19 7.793786793168486E-4 20-21 1.833832186627879E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 1.9866515355135357E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 4.5845804665696974E-5 76-77 1.2989644655280808E-4 78-79 3.8204837221414147E-5 80-81 1.528193488856566E-5 82-83 7.64096744428283E-6 84-85 4.50817079212687E-4 86-87 9.780438328682022E-4 88-89 3.362025675484445E-4 90-91 0.0011002993119767273 92-93 0.001444142846969455 94-95 0.0010238896375338992 96-97 0.0018643960564050106 98-99 6.72405135096889E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6543674.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.11672504083316 #Duplication Level Percentage of deduplicated Percentage of total 1 72.87685085173953 29.23580587461043 2 14.28473446074837 11.46113529286313 3 4.927314242808394 5.93003132005576 4 2.2730637046247657 3.647514865549173 5 1.2728880626087564 2.553205020771715 6 0.8363840703617351 2.0131793867540755 7 0.5868259448861872 1.6479074554478401 8 0.4110978899154939 1.3193520811685253 9 0.30028003205924625 1.0841626333236014 >10 1.9007023793538251 14.259942554258027 >50 0.17405390203548696 4.857179976567984 >100 0.13051242613844355 10.559711867599377 >500 0.016212876919807814 4.473389951207298 >1k 0.008888417232734166 6.2330698801428985 >5k 1.5259085376709942E-4 0.48016617123971633 >10k+ 3.8147713441774855E-5 0.24424566844039622 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15960 0.24389968082150792 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9939 0.15188715085745408 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8896 0.1359480927686801 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.528193488856566E-5 0.0 2 0.0 3.056386977713132E-5 0.0 1.528193488856566E-5 0.0 3 0.0 3.056386977713132E-5 0.0 1.528193488856566E-5 0.0 4 0.0 4.5845804665696974E-5 0.0 1.528193488856566E-5 0.0 5 1.528193488856566E-5 1.2225547910852527E-4 0.0 1.528193488856566E-5 1.528193488856566E-5 6 1.528193488856566E-5 1.2225547910852527E-4 0.0 1.528193488856566E-5 6.112773955426264E-5 7 1.528193488856566E-5 1.2225547910852527E-4 0.0 1.528193488856566E-5 6.112773955426264E-5 8 1.528193488856566E-5 1.2225547910852527E-4 0.0 1.528193488856566E-5 6.112773955426264E-5 9 1.528193488856566E-5 1.2225547910852527E-4 0.0 1.528193488856566E-5 6.112773955426264E-5 10-11 1.528193488856566E-5 1.3753741399709094E-4 0.0 1.528193488856566E-5 6.112773955426264E-5 12-13 1.528193488856566E-5 2.0630612099563637E-4 0.0 2.292290233284849E-5 6.112773955426264E-5 14-15 1.528193488856566E-5 2.4451095821705055E-4 0.0 3.056386977713132E-5 7.640967444282829E-5 16-17 3.056386977713132E-5 2.4451095821705055E-4 0.0 3.056386977713132E-5 9.169160933139395E-5 18-19 4.5845804665696974E-5 2.597928931056162E-4 0.0 3.8204837221414147E-5 1.0697354421995962E-4 20-21 4.5845804665696974E-5 3.0563869777131317E-4 0.0 1.6046031632993942E-4 1.0697354421995962E-4 22-23 4.5845804665696974E-5 3.0563869777131317E-4 0.0 9.016341584253738E-4 1.0697354421995962E-4 24-25 4.5845804665696974E-5 5.730725583212122E-4 0.0 0.0026284928008332934 1.0697354421995962E-4 26-27 4.5845804665696974E-5 6.26559330431192E-4 0.0 0.005027756578338102 1.0697354421995962E-4 28-29 4.5845804665696974E-5 6.418412653197577E-4 0.0 0.01703935740075071 1.0697354421995962E-4 30-31 4.5845804665696974E-5 6.72405135096889E-4 0.0 0.04781717426632195 1.0697354421995962E-4 32-33 4.5845804665696974E-5 6.876870699854547E-4 0.0 0.10001262287821795 1.0697354421995962E-4 34-35 5.34867721099798E-5 7.18250939762586E-4 0.0 0.16792554152300374 1.0697354421995962E-4 36-37 6.112773955426264E-5 8.405064188711112E-4 0.0 0.26948163982496687 1.0697354421995962E-4 38-39 6.112773955426264E-5 9.474799630910709E-4 0.0 0.4149427370617791 1.0697354421995962E-4 40-41 7.640967444282829E-5 9.933257677567679E-4 0.0 0.5841290382130895 1.0697354421995962E-4 42-43 8.405064188711113E-5 0.0010391715724224649 0.0 0.7476228186184092 1.0697354421995962E-4 44-45 9.169160933139395E-5 0.0010697354421995962 0.0 0.9401369933771151 1.0697354421995962E-4 46-47 9.933257677567678E-5 0.0011002993119767273 0.0 1.1557345307849993 1.0697354421995962E-4 48-49 1.0697354421995962E-4 0.0011308631817538587 0.0 1.376932897329543 1.2225547910852527E-4 50-51 1.0697354421995962E-4 0.00116142705153099 0.0 1.6115946485109132 1.5281934888565659E-4 52-53 1.1461451166424244E-4 0.001207272856196687 0.0 1.8476852606043639 1.757422512185051E-4 54-55 1.2225547910852527E-4 0.0013524512376380609 0.0 2.0899803382625723 2.2922902332848488E-4 56-57 1.2225547910852527E-4 0.0014670657493023032 0.0 2.3692729802859986 2.2922902332848488E-4 58-59 1.2225547910852527E-4 0.0015893212284108285 0.0 2.6684550605668926 2.2922902332848488E-4 60-61 1.2225547910852527E-4 0.0016275260656322427 0.0 2.9724662322725734 2.2922902332848488E-4 62-63 1.2225547910852527E-4 0.0016351670330765255 0.0 3.2847754946227457 2.4451095821705055E-4 64-65 1.2225547910852527E-4 0.001658089935409374 0.0 3.610739165795851 2.597928931056162E-4 66-67 1.2225547910852527E-4 0.0016657309028536569 0.0 3.9679543938160737 2.597928931056162E-4 68-69 1.2225547910852527E-4 0.0017192176749636367 0.0 4.349360924764895 2.597928931056162E-4 70-71 1.2225547910852527E-4 0.0017727044470736164 0.0 4.769102189381684 2.597928931056162E-4 72-73 1.2225547910852527E-4 0.0018185502517393134 0.0 5.186314293774414 3.2092063265987884E-4 74-75 1.2225547910852527E-4 0.0018185502517393134 0.0 5.61227530589085 3.4384353499272735E-4 76-77 1.2225547910852527E-4 0.001833832186627879 0.0 6.070229048696497 3.514845024370102E-4 78-79 1.2225547910852527E-4 0.0018873189587378589 0.0 6.551380768662987 3.514845024370102E-4 80-81 1.2225547910852527E-4 0.0019178828285149902 0.0 7.068544368194381 3.514845024370102E-4 82-83 1.2225547910852527E-4 0.0019331647634035559 0.0 7.608348154263187 3.59125469881293E-4 84-85 1.2225547910852527E-4 0.0019484466982921216 0.0 8.146165288796478 3.667664373255758E-4 86-87 1.2225547910852527E-4 0.0019866515355135357 0.0 8.707226246295278 3.896893396584243E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 570 0.0 35.079983 2 GTATCAA 25480 0.0 34.67637 1 ATCAACG 32160 0.0 27.227016 3 GGTATCA 16000 0.0 26.92769 1 CAACGCA 32635 0.0 26.787983 5 TCAACGC 34015 0.0 25.783209 4 AACGCAG 34525 0.0 25.629944 6 TATCAAC 35060 0.0 25.240192 2 ACGCAGA 37990 0.0 23.035244 7 CGCAGAG 38475 0.0 22.75696 8 TAACGCA 460 0.0 22.239786 4 GCAGAGT 41035 0.0 21.17909 9 AGTACTT 23025 0.0 20.813606 12-13 AGAGTAC 39085 0.0 19.917324 10-11 CTAACGC 830 0.0 18.488497 3 GAGTACT 21780 0.0 18.29877 12-13 ATCACGC 1095 0.0 18.260813 3 TACTTTT 26695 0.0 18.056698 14-15 TCACGCA 1140 0.0 17.947899 4 GTACTAG 1745 0.0 17.597727 1 >>END_MODULE