##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765374_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7667004 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.454470220701594 34.0 31.0 34.0 31.0 34.0 2 32.588034126498435 34.0 31.0 34.0 31.0 34.0 3 32.63688775954728 34.0 31.0 34.0 31.0 34.0 4 36.1594009863566 37.0 35.0 37.0 35.0 37.0 5 36.09452036284316 37.0 35.0 37.0 35.0 37.0 6 36.15429012427801 37.0 36.0 37.0 35.0 37.0 7 36.10482803974016 37.0 36.0 37.0 35.0 37.0 8 36.11876555692419 37.0 36.0 37.0 35.0 37.0 9 37.84009529667651 39.0 38.0 39.0 35.0 39.0 10-11 37.78650063310258 39.0 38.0 39.0 35.0 39.0 12-13 37.75570685759392 39.0 38.0 39.0 35.0 39.0 14-15 39.105321126739994 40.0 38.5 41.0 36.0 41.0 16-17 39.07100348454233 40.0 38.0 41.0 36.0 41.0 18-19 39.09019168373983 40.0 38.5 41.0 36.0 41.0 20-21 39.07470675638098 40.0 39.0 41.0 35.5 41.0 22-23 39.01628687033423 40.0 38.5 41.0 35.0 41.0 24-25 38.95987806971276 40.0 38.0 41.0 35.0 41.0 26-27 38.82022097549447 40.0 38.0 41.0 35.0 41.0 28-29 38.67771511531753 40.0 38.0 41.0 35.0 41.0 30-31 38.49691757040951 40.0 38.0 41.0 34.0 41.0 32-33 38.236830579454505 40.0 38.0 41.0 34.0 41.0 34-35 38.16832037651213 40.0 38.0 41.0 34.0 41.0 36-37 38.06837710010325 40.0 38.0 41.0 33.5 41.0 38-39 37.90224949667432 40.0 37.0 41.0 33.0 41.0 40-41 37.68999631407522 40.0 37.0 41.0 33.0 41.0 42-43 37.45594276460531 40.0 37.0 41.0 33.0 41.0 44-45 37.142584378461265 40.0 36.0 41.0 32.0 41.0 46-47 36.82798717725986 39.0 36.0 41.0 31.0 41.0 48-49 36.56706954893984 39.0 35.5 41.0 31.0 41.0 50-51 36.32803537079151 39.0 35.0 41.0 30.5 41.0 52-53 36.20732446468007 39.0 35.0 41.0 30.5 41.0 54-55 36.20307326042872 39.0 35.0 41.0 31.0 41.0 56-57 36.0397680762916 39.0 35.0 41.0 30.0 41.0 58-59 35.921177816002185 39.0 35.0 41.0 30.0 41.0 60-61 35.595382172749616 38.0 35.0 40.0 30.0 41.0 62-63 35.2625150580331 37.0 35.0 40.0 29.0 41.0 64-65 35.06210365874337 37.0 35.0 40.0 29.0 41.0 66-67 34.71686534922898 36.5 35.0 39.0 29.0 41.0 68-69 34.378691663653754 36.0 34.5 39.0 29.0 41.0 70-71 33.99810414863485 35.0 34.0 38.5 29.0 40.5 72-73 33.61816010530319 35.0 34.0 37.5 28.5 39.5 74-75 33.26477839322896 35.0 34.0 37.0 28.0 39.0 76-77 31.807947993766536 34.5 32.0 35.5 25.5 37.5 78-79 32.60280332447981 35.0 33.5 36.0 27.0 37.5 80-81 32.55669978781803 35.0 34.0 35.5 27.0 37.0 82-83 32.3905092523755 35.0 34.0 35.0 27.0 37.0 84-85 32.19830940482097 35.0 34.0 35.0 27.0 36.0 86-87 32.013624291835505 35.0 34.0 35.0 26.5 36.0 88-89 31.85577704146235 35.0 33.0 35.0 26.0 36.0 90-91 31.716470670942652 35.0 33.0 35.0 25.5 35.5 92-93 31.561751043823637 35.0 33.0 35.0 25.0 35.0 94-95 31.46518646657808 35.0 33.0 35.0 25.0 35.0 96-97 31.3477756109166 35.0 33.0 35.0 24.5 35.0 98-99 31.20751932567141 35.0 33.0 35.0 24.0 35.0 100 30.8631897153047 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41547574336653526 1101 2 0.3892225170653276 1101 3 0.4038760888406898 1101 4 0.2799072957909772 1101 5 0.28400971202522385 1101 6 0.2843209581291575 1101 7 0.3163223362121528 1101 8 0.29143640414554994 1101 9 0.35028229113780185 1101 10-11 0.3990922404058921 1101 12-13 0.43959789356127743 1101 14-15 0.5492332671608722 1101 16-17 0.5494707419068092 1101 18-19 0.5062414308839323 1101 20-21 0.5345369033868437 1101 22-23 0.5329823266885398 1101 24-25 0.5788172023490432 1101 26-27 0.5716670017680698 1101 28-29 0.5713927818338576 1101 30-31 0.6439725348783725 1101 32-33 0.7031611057467799 1101 34-35 0.573635815521456 1101 36-37 0.6829887553664094 1101 38-39 0.6940791120783487 1101 40-41 0.6804523007945633 1101 42-43 0.715962099608781 1101 44-45 0.7523750615848499 1101 46-47 0.7680864282902604 1101 48-49 0.7551219601198937 1101 50-51 0.7853998925308261 1101 52-53 0.7693540652812274 1101 54-55 0.8692384268006137 1101 56-57 0.8574890404739293 1101 58-59 0.9436897955540289 1101 60-61 0.968949894192356 1101 62-63 0.9767952405801452 1101 64-65 0.8860582653578462 1101 66-67 0.9607792798535115 1101 68-69 0.9970747509412661 1101 70-71 1.0298233912938386 1101 72-73 1.0111199846540515 1101 74-75 0.9874321852146934 1101 76-77 1.0322508303706321 1101 78-79 0.9625566857170824 1101 80-81 1.0035326024329052 1101 82-83 0.9138697253542531 1101 84-85 0.952066481689684 1101 86-87 0.9735584956984127 1101 88-89 1.018896374109783 1101 90-91 0.97562999131749 1101 92-93 1.0435151468571142 1101 94-95 1.047638081886582 1101 96-97 1.0800048987858482 1101 98-99 1.0678150599475948 1101 100 1.1047962514218028 1102 1 0.3772148502438668 1102 2 0.29625324693225963 1102 3 0.34097695981859033 1102 4 0.22065016789009917 1102 5 0.24261652315720994 1102 6 0.26600302519528185 1102 7 0.2854532270087802 1102 8 0.29918940233321223 1102 9 0.3445156072988951 1102 10-11 0.3336031104064787 1102 12-13 0.34381118854764026 1102 14-15 0.43305244825025824 1102 16-17 0.46286896563564284 1102 18-19 0.4518767172066802 1102 20-21 0.46588944254109066 1102 22-23 0.5129169833187035 1102 24-25 0.45811953009825856 1102 26-27 0.5375553390769454 1102 28-29 0.4999795921822354 1102 30-31 0.514269994828382 1102 32-33 0.5440156702498271 1102 34-35 0.5467808091135424 1102 36-37 0.5924789465783817 1102 38-39 0.5299219378459199 1102 40-41 0.5886739817779798 1102 42-43 0.650073085023152 1102 44-45 0.6757626076451331 1102 46-47 0.7807795049633697 1102 48-49 0.7342024833737142 1102 50-51 0.7182861722292344 1102 52-53 0.8023171612720219 1102 54-55 0.8220057650490844 1102 56-57 0.8672126227115697 1102 58-59 0.836458242205957 1102 60-61 0.864224556132946 1102 62-63 0.8409771827506205 1102 64-65 0.8926456192754415 1102 66-67 0.9032506641824369 1102 68-69 0.8651173526686051 1102 70-71 0.8198791770506588 1102 72-73 0.7881950331812888 1102 74-75 0.7735671211741746 1102 76-77 0.8718088104313217 1102 78-79 0.8246036403676484 1102 80-81 0.7818658563864958 1102 82-83 0.8554852284958727 1102 84-85 0.8253227671456713 1102 86-87 0.811377695139818 1102 88-89 0.8638656861903193 1102 90-91 0.8816792524959425 1102 92-93 0.840754362899812 1102 94-95 0.9089094454165547 1102 96-97 0.9262052888098715 1102 98-99 0.8882236224153068 1102 100 0.8821604534273852 1103 1 0.35184160936627507 1103 2 0.25974873752198135 1103 3 0.3269938380966835 1103 4 0.23815122314564263 1103 5 0.24421009392199977 1103 6 0.25878931073311406 1103 7 0.26373910462514516 1103 8 0.25884059454716635 1103 9 0.3121724917362698 1103 10-11 0.34780956169448274 1103 12-13 0.3358863957906877 1103 14-15 0.43967202374907544 1103 16-17 0.42206395264953045 1103 18-19 0.4179780768019441 1103 20-21 0.3954744293156338 1103 22-23 0.41583862656499093 1103 24-25 0.44542256808594516 1103 26-27 0.5010079415356543 1103 28-29 0.4783955868541483 1103 30-31 0.47716017478835937 1103 32-33 0.48408376138350206 1103 34-35 0.5284266259228403 1103 36-37 0.6114422449910961 1103 38-39 0.6505942660225728 1103 40-41 0.666102553325139 1103 42-43 0.6439124125261841 1103 44-45 0.6221408917518829 1103 46-47 0.6503063741699364 1103 48-49 0.6598244684937455 1103 50-51 0.6740842809179881 1103 52-53 0.7220806276955258 1103 54-55 0.7137689888087735 1103 56-57 0.6650760536209361 1103 58-59 0.7056466410495332 1103 60-61 0.7096439377220705 1103 62-63 0.6971605513698194 1103 64-65 0.7416246119771301 1103 66-67 0.7239758554392921 1103 68-69 0.6875897856306494 1103 70-71 0.5823813093516961 1103 72-73 0.6682594124164112 1103 74-75 0.6760256417799084 1103 76-77 0.6604655431000488 1103 78-79 0.7102556859170548 1103 80-81 0.6874332822699216 1103 82-83 0.6677074673199499 1103 84-85 0.658033738358462 1103 86-87 0.5939538592724176 1103 88-89 0.6410313396594809 1103 90-91 0.6699217342411643 1103 92-93 0.7034065068152238 1103 94-95 0.724826738837919 1103 96-97 0.7091216872527788 1103 98-99 0.6789705634885372 1103 100 0.7529365086241349 1104 1 0.27791478068665043 1104 2 0.17801838693852545 1104 3 0.23185942081141064 1104 4 0.19436711048347632 1104 5 0.2096201353274978 1104 6 0.19422387380112838 1104 7 0.20240255959470943 1104 8 0.22137412284661906 1104 9 0.23943712731548317 1104 10-11 0.2824569467570299 1104 12-13 0.30718213432700026 1104 14-15 0.3686484278546658 1104 16-17 0.3368595435606565 1104 18-19 0.3189384804466684 1104 20-21 0.33822769383724705 1104 22-23 0.3608588328534239 1104 24-25 0.37653640063042104 1104 26-27 0.3460640651588065 1104 28-29 0.3740660776029543 1104 30-31 0.4578472136055538 1104 32-33 0.4422610336577364 1104 34-35 0.4208809801863538 1104 36-37 0.43292276407317587 1104 38-39 0.43536243570662947 1104 40-41 0.4033602363688047 1104 42-43 0.4725792435126337 1104 44-45 0.5001310833369814 1104 46-47 0.45537523695245596 1104 48-49 0.4130339354803354 1104 50-51 0.43091269084822414 1104 52-53 0.456736216241481 1104 54-55 0.44809282189973487 1104 56-57 0.39865678992442355 1104 58-59 0.4406532744912539 1104 60-61 0.46410366476699494 1104 62-63 0.4635734269371028 1104 64-65 0.39726563854803487 1104 66-67 0.4084671533900206 1104 68-69 0.45474004722714767 1104 70-71 0.4296293428812632 1104 72-73 0.4301622062235637 1104 74-75 0.4119604610191985 1104 76-77 0.3541171369914444 1104 78-79 0.4113178640277724 1104 80-81 0.44917601852930744 1104 82-83 0.407308259889831 1104 84-85 0.4221012013382577 1104 86-87 0.4554384275738954 1104 88-89 0.496568913968634 1104 90-91 0.4967961582934777 1104 92-93 0.5026114942330047 1104 94-95 0.5432800454065472 1104 96-97 0.5491856702548965 1104 98-99 0.5669410346165051 1104 100 0.5784507717847944 1105 1 0.14610156087680082 1105 2 0.15237384998075498 1105 3 0.1691360726864417 1105 4 0.1670471132602529 1105 5 0.14938346858036056 1105 6 0.1663713853208506 1105 7 0.1497023829086146 1105 8 0.13146119962404867 1105 9 0.1852250910366422 1105 10-11 0.18735817529324805 1105 12-13 0.2295118319541487 1105 14-15 0.19098790432311574 1105 16-17 0.20651470930168614 1105 18-19 0.18440602883104162 1105 20-21 0.24828943580678953 1105 22-23 0.29110639535275595 1105 24-25 0.24773188887405695 1105 26-27 0.2505008410043743 1105 28-29 0.29378600820605527 1105 30-31 0.2848907260676583 1105 32-33 0.23454818627990193 1105 34-35 0.2855518113305635 1105 36-37 0.24495754931864155 1105 38-39 0.24585982928375927 1105 40-41 0.21486427758534887 1105 42-43 0.26444297512411197 1105 44-45 0.2731845232487231 1105 46-47 0.2511207834229481 1105 48-49 0.24926426072872943 1105 50-51 0.24602039008413357 1105 52-53 0.2860084778770542 1105 54-55 0.3310792819150308 1105 56-57 0.38106462622380377 1105 58-59 0.2989276819296336 1105 60-61 0.28375961488193724 1105 62-63 0.2806024853135938 1105 64-65 0.21213507598660897 1105 66-67 0.21713737845272618 1105 68-69 0.2483743691001976 1105 70-71 0.22014664734412293 1105 72-73 0.22008705944465845 1105 74-75 0.25543568649852233 1105 76-77 0.23756608641416577 1105 78-79 0.27794906634711225 1105 80-81 0.27594430498604083 1105 82-83 0.3142435062062532 1105 84-85 0.33009957021625524 1105 86-87 0.3491673909706918 1105 88-89 0.4025500985761603 1105 90-91 0.3760243399935561 1105 92-93 0.3942135549476795 1105 94-95 0.4063677657463458 1105 96-97 0.45924190751645355 1105 98-99 0.4639336886494583 1105 100 0.495800305617756 1106 1 0.1586259942624011 1106 2 0.1185031476561349 1106 3 0.19979899069166152 1106 4 0.139930405421822 1106 5 0.1811088533796692 1106 6 0.13809487520136798 1106 7 0.15935226123050938 1106 8 0.18970510681712227 1106 9 0.20032012261381027 1106 10-11 0.20597201682642918 1106 12-13 0.22204934255400843 1106 14-15 0.33518502171946096 1106 16-17 0.29553054044709626 1106 18-19 0.25214334794437576 1106 20-21 0.2977250850732389 1106 22-23 0.31798683323821564 1106 24-25 0.31915772078146887 1106 26-27 0.3035470366924571 1106 28-29 0.37429615603858934 1106 30-31 0.34671670340964056 1106 32-33 0.28891246158413253 1106 34-35 0.30060686239582424 1106 36-37 0.3097592052992013 1106 38-39 0.35189920492639004 1106 40-41 0.3567368125396442 1106 42-43 0.3828246672340967 1106 44-45 0.31964399521377374 1106 46-47 0.2761635700068936 1106 48-49 0.28498538712234733 1106 50-51 0.27015473545224467 1106 52-53 0.36249256191521795 1106 54-55 0.37482508319579466 1106 56-57 0.40581681485063115 1106 58-59 0.3822932519200677 1106 60-61 0.3564982073352141 1106 62-63 0.3517917410715512 1106 64-65 0.39183234215069973 1106 66-67 0.39033307850930044 1106 68-69 0.398617808425044 1106 70-71 0.41654588171294904 1106 72-73 0.3829368878302759 1106 74-75 0.3880691100839968 1106 76-77 0.2580853798981586 1106 78-79 0.41213082055374883 1106 80-81 0.3553613751248932 1106 82-83 0.3761364705897492 1106 84-85 0.3578288634824176 1106 86-87 0.40074978376321013 1106 88-89 0.4704219166807526 1106 90-91 0.4750497165955423 1106 92-93 0.4265496967442921 1106 94-95 0.5044540027481901 1106 96-97 0.526311350866127 1106 98-99 0.5268517638563885 1106 100 0.5798179953758051 1107 1 0.14237637725253904 1107 2 0.04905004020707082 1107 3 0.09974866104634117 1107 4 0.05654291810690637 1107 5 0.14439803009845775 1107 6 0.12510955624504305 1107 7 0.07384505540633057 1107 8 0.0967702296985209 1107 9 0.13466686498719582 1107 10-11 0.16426224568289882 1107 12-13 0.17841572213723822 1107 14-15 0.1787642037780799 1107 16-17 0.18667063630214642 1107 18-19 0.13822987726972968 1107 20-21 0.19122451035430288 1107 22-23 0.17511359015120576 1107 24-25 0.2099084405491567 1107 26-27 0.2287159313355076 1107 28-29 0.2419271102830578 1107 30-31 0.23351171440049256 1107 32-33 0.2582612801847759 1107 34-35 0.2548406013091409 1107 36-37 0.2819101460191362 1107 38-39 0.2453370662961163 1107 40-41 0.2662712868608068 1107 42-43 0.3594817022720562 1107 44-45 0.3112607268006613 1107 46-47 0.3170117218641124 1107 48-49 0.3019892893477376 1107 50-51 0.30786108265727563 1107 52-53 0.32078628008897425 1107 54-55 0.35984444239552715 1107 56-57 0.3382278626368489 1107 58-59 0.3396920269927364 1107 60-61 0.304795148369287 1107 62-63 0.3271827466200037 1107 64-65 0.2964807622145429 1107 66-67 0.3313147493426669 1107 68-69 0.26417756904385925 1107 70-71 0.3062847328600711 1107 72-73 0.27056674719710117 1107 74-75 0.2158427617116203 1107 76-77 0.14451595757683577 1107 78-79 0.24960522499240057 1107 80-81 0.2674060960182416 1107 82-83 0.28522988852861175 1107 84-85 0.2872739832331135 1107 86-87 0.28886232923785826 1107 88-89 0.27360290185825775 1107 90-91 0.28737077639168973 1107 92-93 0.2624989189303939 1107 94-95 0.30441823808084223 1107 96-97 0.3130028994258005 1107 98-99 0.3367420300034816 1107 100 0.39494202875144424 1108 1 0.07574943665508727 1108 2 -0.013114905242325392 1108 3 -0.008122707667432394 1108 4 0.05516724955892727 1108 5 0.0531729077850116 1108 6 0.05220653850347645 1108 7 0.06392012948128922 1108 8 0.02654355043191714 1108 9 0.019019121476496537 1108 10-11 0.08459169192378369 1108 12-13 0.06062173664937376 1108 14-15 0.05245403706128826 1108 16-17 0.0709378770085678 1108 18-19 0.07305503486440301 1108 20-21 0.11555174735566709 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-0.807536652862332 2316 52-53 -0.8911005775080625 2316 54-55 -0.9248030219033723 2316 56-57 -0.8508703263411022 2316 58-59 -0.8639425810742765 2316 60-61 -0.7478698456788919 2316 62-63 -0.6900601042245142 2316 64-65 -0.7270555204468963 2316 66-67 -0.6938855932954908 2316 68-69 -0.7095370965319745 2316 70-71 -0.7083706790009288 2316 72-73 -0.711106350019115 2316 74-75 -0.6769167596715491 2316 76-77 -0.719033671453392 2316 78-79 -0.7763899964453032 2316 80-81 -0.7620643790636663 2316 82-83 -0.7527862044810192 2316 84-85 -0.7570465473351717 2316 86-87 -0.7461454505139287 2316 88-89 -0.7152932582057581 2316 90-91 -0.741767170011002 2316 92-93 -0.7799903679062616 2316 94-95 -0.7833299141531924 2316 96-97 -0.84512687581298 2316 98-99 -0.9262330175827316 2316 100 -1.0031088707336622 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25.0 3 29.0 4 53.0 5 132.0 6 292.0 7 781.0 8 1703.0 9 2945.0 10 5205.0 11 9266.0 12 15379.0 13 21883.0 14 24595.0 15 30185.0 16 39690.0 17 52151.0 18 52126.0 19 34688.0 20 24991.0 21 22375.0 22 23899.0 23 27224.0 24 31318.0 25 35175.0 26 43056.0 27 55342.0 28 70756.0 29 90076.0 30 113763.0 31 143160.0 32 183272.0 33 242350.0 34 346073.0 35 565591.0 36 1071020.0 37 2046582.0 38 1875430.0 39 364109.0 40 314.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80722900366297 18.941844819697497 13.82802200181453 23.422904174825003 2 14.900212087568896 23.012382150190263 36.29632539461195 25.791080367628894 3 19.5952622431495 27.69528804855068 29.580060800551905 23.12938890774791 4 13.820372450117588 18.83089220286101 34.80264213405543 32.54609321296597 5 13.676646053660596 37.588998257989694 32.86051500690491 15.873840681444799 6 30.933021555747196 34.29772568267866 18.28163908614108 16.487613675433064 7 25.4331026826124 32.82998156776754 22.43181300022799 19.30510274939207 8 27.773990987874797 31.459694034332053 21.558551423737356 19.207763554055795 9 28.19971138661203 14.996287989415421 19.818119307098314 36.98588131687423 10-11 25.071400184452923 26.435741399142326 25.738877946606735 22.75398046979802 12-13 27.161430180646523 24.212254366593303 26.248907736211198 22.37740771654898 14-15 25.836250552776647 23.521700310637485 25.059686182641382 25.582362953944486 16-17 23.52029856909556 26.669333083256237 27.222869950302393 22.587498397345808 18-19 24.89965441520573 25.70530809687852 28.381835981825493 21.013201506090255 20-21 24.730410227230117 25.439149056889864 27.623968970054275 22.206471745825745 22-23 23.308988659623374 26.61266859993615 25.329138966814963 24.749203773625513 24-25 23.526000247947795 27.246619298333822 24.861520634741137 24.36585981897724 26-27 24.36186764160283 25.56082991656345 25.39669061943493 24.68061182239879 28-29 23.879593924639277 24.756429066758546 27.888740605116485 23.475236403485688 30-31 25.50150417830205 25.00696345631603 26.768582580722455 22.722949784659473 32-33 22.31967643268852 26.105799791063028 27.503880150863996 24.070643625384452 34-35 22.261506636337074 26.60991824575316 27.640506959806338 23.488068158103424 36-37 24.15100300213579 26.60799611723858 26.159237696333438 23.08176318429219 38-39 24.816715411035847 25.08811649344454 26.050219145857785 24.044948949661833 40-41 23.54872964463805 26.39050613417014 25.98347226226614 24.07729195892567 42-43 25.79495628515691 25.31395312209889 25.808534328482608 23.08255626426159 44-45 25.5149220306389 24.873907425942868 26.523669144563456 23.08750139885478 46-47 23.93448195174175 25.337929580841323 28.07610677366955 22.65148169374738 48-49 24.5401756070788 26.196644242867777 27.31075623682422 21.9524239132292 50-51 23.291689413359347 26.918314922832543 25.97005255928906 23.81994310451905 52-53 22.875881743489554 27.30123327672766 24.458301435846693 25.364583543936092 54-55 23.83218438530351 26.45724250777026 24.32896719385024 25.381605913075987 56-57 24.559756255388077 24.99328121118758 24.79890599853465 25.64805653488969 58-59 24.16092668126473 24.885680834719732 26.81899567694167 24.13439680707387 60-61 24.398183273063438 25.504534935503482 25.986909374413536 24.110372417019548 62-63 21.972672873424532 26.21612561347443 26.771420651872624 25.039780861228415 64-65 22.095674492493465 26.219837257203558 26.33693182492608 25.3475564253769 66-67 23.940221947465858 26.09733840294264 25.145355197528836 24.81708445206267 68-69 24.460338476271147 25.04619539946385 25.191469447386027 25.30199667687898 70-71 23.2356265207249 25.720677684904825 25.57223602237385 25.471459771996425 72-73 24.597575051749875 25.026038791424437 25.660158604624684 24.716227552201005 74-75 24.78602929830445 24.575446675293712 26.04413150206213 24.59439252433971 76-77 23.42022611426753 25.64725469940993 26.81664866726143 24.115870519061108 78-79 23.534173260656353 25.5610176403259 26.732782971356002 24.172026127661745 80-81 22.630208618646865 25.76689669132819 26.307062054486995 25.295832635537952 82-83 22.70421193057731 26.490976521373472 24.658838110064995 26.145973437984228 84-85 23.0401746914554 26.007305200786725 24.66487722354633 26.287642884211543 86-87 23.112119565543466 24.698778837708517 25.479010058487344 26.710091538260677 88-89 23.275294758437738 24.196437497672655 26.447794400898868 26.08047334299074 90-91 23.72650517095905 24.757794915398595 25.99177274205281 25.52392717158955 92-93 22.125453718525673 25.403274560081012 26.411312595678865 26.05995912571445 94-95 21.580786447681454 25.681541904920905 26.687355221419928 26.05031642597771 96-97 22.870811662232178 25.226156267222784 25.678772049808085 26.224260020736956 98-99 23.11647417948393 24.566623416395768 25.653234301169007 26.6636681029513 100 23.003109837942265 25.27429714962608 25.907194756095603 25.815398256336053 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1277.0 1 800.5 2 430.5 3 555.0 4 485.0 5 587.5 6 942.0 7 1833.5 8 2833.0 9 2932.5 10 2289.5 11 2003.0 12 2285.0 13 2555.0 14 2908.0 15 3336.5 16 3764.5 17 4323.5 18 5017.0 19 5784.0 20 6642.0 21 7681.5 22 9094.0 23 10988.5 24 13388.5 25 16651.0 26 21398.0 27 27046.5 28 33542.0 29 42758.5 30 51937.5 31 60342.0 32 71195.5 33 83077.0 34 94471.5 35 102885.0 36 111375.5 37 122412.0 38 128822.5 39 132557.0 40 136600.5 41 140144.5 42 146265.5 43 161431.5 44 180772.5 45 197128.5 46 226312.0 47 270764.0 48 402533.5 49 544327.0 50 680097.5 51 640598.5 52 414621.0 53 308377.5 54 268866.5 55 239714.0 56 220742.0 57 203095.0 58 192638.5 59 177487.5 60 150366.5 61 120824.5 62 95584.5 63 73774.5 64 53994.0 65 44040.0 66 36468.0 67 27311.0 68 20339.5 69 16459.0 70 13780.5 71 11687.5 72 10582.5 73 11235.5 74 8735.5 75 6246.5 76 4972.5 77 3648.0 78 2720.0 79 1677.0 80 1186.0 81 1093.5 82 965.0 83 833.0 84 605.5 85 335.0 86 199.5 87 99.5 88 52.0 89 34.0 90 23.5 91 15.5 92 10.5 93 6.0 94 3.5 95 1.5 96 2.0 97 2.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.060036488829274114 3 0.025329320292515826 4 0.006182336672838569 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.130033061154005E-5 12-13 1.8912211340961867E-4 14-15 3.7172277463269875E-4 16-17 4.565016530577002E-4 18-19 0.0 20-21 0.002823788796771203 22-23 0.004845438974598161 24-25 8.803960451827076E-4 26-27 5.282376271096245E-4 28-29 0.005184554488298168 30-31 0.0025238019961904288 32-33 0.0012847260807480993 34-35 0.0016173201422615667 36-37 0.003854178242244298 38-39 0.003997650190348146 40-41 9.716963757942477E-4 42-43 0.0027977029880250485 44-45 2.347722787153887E-4 46-47 0.0022172937434231156 48-49 1.3042904373077148E-5 50-51 0.0018064422556711853 52-53 0.006743181560880887 54-55 0.014066772366363707 56-57 0.025198891248785056 58-59 0.02686838300853893 60-61 0.024788039761033123 62-63 0.008203986850665528 64-65 0.00718664030956551 66-67 1.2390759154423293E-4 68-69 0.001499934002903872 70-71 2.4781518308846585E-4 72-73 0.001010825088913479 74-75 0.005438891123573171 76-77 0.0010956039673384807 78-79 0.001441240933225025 80-81 0.0 82-83 0.0015129769072769494 84-85 6.521452186538574E-6 86-87 5.999736011615489E-4 88-89 7.173597405192433E-5 90-91 6.651881230269346E-4 92-93 0.001056475254219249 94-95 0.0016173201422615667 96-97 2.0216501778269583E-4 98-99 0.0 100 5.869306967884718E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7667004.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.053932811994244 #Duplication Level Percentage of deduplicated Percentage of total 1 78.90016445074632 29.235613924132505 2 11.97922935137333 8.877551190505136 3 3.6109004626007017 4.01394189396016 4 1.6328750595473378 2.4201777098739266 5 0.8649875500066171 1.6025595280578349 6 0.5559827331426183 1.23608081030973 7 0.35258264900959496 0.9145201650953536 8 0.2877067029062631 0.852853187123925 9 0.20558773236544903 0.6856050619835642 >10 1.3003748652622094 9.265868892037282 >50 0.14827560355890793 3.8430847546453006 >100 0.1313595460937319 10.044559795972802 >500 0.017573016971532386 4.4899609252303145 >1k 0.010380802827793042 7.10706377777935 >5k 8.85734029655929E-4 2.5648380849536374 >10k+ 0.001133739557959589 12.845720298339241 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 117140 1.5278458182622574 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 94129 1.227715545733379 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 60456 0.7885218267787523 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 54809 0.7148685457839855 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 50855 0.6632969018928385 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 50507 0.6587579711710077 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 49576 0.6466150271996728 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 44703 0.5830569541896679 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 39601 0.5165120560782283 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 36182 0.47191836602667747 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 33168 0.4326070522462229 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 32669 0.4260986429640574 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 24897 0.3247291901765018 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 21186 0.2763269720480125 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 20081 0.26191456271576224 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 19146 0.2497194471269351 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 18846 0.24580657581501197 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18605 0.2426632358611004 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 18002 0.23479836452413483 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 17631 0.22995944700172324 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 15583 0.20324757884566122 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 14105 0.1839701661822532 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 13742 0.17923559189482619 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 13708 0.17879213314614156 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 13402 0.17480100440797997 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 13256 0.1728967403695107 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 12892 0.1681491231777106 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 12581 0.16409277991768362 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12488 0.16287978981098744 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 12211 0.15926690529964507 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11524 0.15030642999534108 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 10819 0.14111118241232168 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 9968 0.13001167079083303 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 9778 0.12753351895994838 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 9642 0.1257596839652099 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 9581 0.12496406679845216 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 9560 0.12469016580661754 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 9493 0.1238162912136214 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 9413 0.1227728588637752 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 9238 0.1204903505984867 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 9088 0.11853391494252513 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 8817 0.11499928785742124 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 8456 0.11029079937874037 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 8192 0.106847472624248 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 7768 0.1013172811700633 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 7757 0.10117380922195944 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002621623778988507 0.0 0.0 1.304290437307715E-5 1.304290437307715E-5 2 0.003965042929415454 0.0 0.0 2.60858087461543E-5 1.304290437307715E-5 3 0.004225901016876996 0.0 0.0 2.60858087461543E-5 1.304290437307715E-5 4 0.004891089139903931 2.60858087461543E-5 0.0 3.912871311923145E-5 1.304290437307715E-5 5 0.02000781530830035 2.60858087461543E-5 0.0 2.9998680058077444E-4 1.304290437307715E-5 6 0.04343287156234691 2.60858087461543E-5 0.0 5.478019836692402E-4 1.304290437307715E-5 7 0.04953695080894702 3.912871311923145E-5 0.0 6.130165055346261E-4 1.304290437307715E-5 8 0.05789745251208947 6.521452186538575E-5 0.0 6.651881230269346E-4 1.304290437307715E-5 9 0.06414500370679342 9.130033061154005E-5 0.0 7.956171667577062E-4 1.304290437307715E-5 10-11 0.1266270371060195 9.130033061154005E-5 0.0 0.0017542706381788765 1.304290437307715E-5 12-13 0.21179068121002675 9.130033061154005E-5 0.0 0.0036715775810212178 1.9564356559615727E-5 14-15 0.30807340129208227 1.043432349846172E-4 0.0 0.0060649505334808744 3.2607260932692873E-5 16-17 0.35030632565210607 1.043432349846172E-4 0.0 0.007101861431140508 3.912871311923145E-5 18-19 0.36797946107762564 1.1086468717115577E-4 0.0 0.007662706319182825 3.912871311923145E-5 20-21 0.4276377056800805 1.1738613935769435E-4 0.0 0.009221333391765545 3.912871311923145E-5 22-23 0.44447609522572307 1.4347194810384865E-4 0.0 0.010766917559975189 3.912871311923145E-5 24-25 0.5176207029499398 1.4347194810384865E-4 0.0 0.017144897798409914 3.912871311923145E-5 26-27 0.6644577203820423 1.4347194810384865E-4 0.0 0.03720488472420257 3.912871311923145E-5 28-29 0.7024777866295622 1.4347194810384865E-4 0.0 0.060492990482331824 3.912871311923145E-5 30-31 0.7189574963049452 1.565148524769258E-4 0.0 0.11047340003996346 3.912871311923145E-5 32-33 0.7342763874911242 1.565148524769258E-4 0.0 0.19980425209116887 6.521452186538575E-5 34-35 0.745219384260136 2.1520792215577294E-4 0.0 0.31923812743543634 6.521452186538575E-5 36-37 0.7644315824016786 2.739009918346201E-4 0.0 0.49121534304664505 7.173597405192431E-5 38-39 0.784269839953129 2.9998680058077444E-4 0.0 0.7604534965678902 7.82574262384629E-5 40-41 0.8035211668077908 3.130297049538516E-4 0.0 1.1520080072998526 7.82574262384629E-5 42-43 0.8449976027141762 3.391155137000059E-4 0.0 1.4450885900150827 8.477887842500147E-5 44-45 0.8896695501919654 3.9128713119231454E-4 0.0 1.7499077866660824 9.130033061154005E-5 46-47 0.9052492994656061 4.2389439212500734E-4 0.0 2.0991054654464767 9.130033061154005E-5 48-49 0.9154488506853524 4.3041584431154594E-4 0.0 2.4129373090192727 1.043432349846172E-4 50-51 0.9349414712709163 4.695445574307774E-4 0.0 2.760935040597344 1.043432349846172E-4 52-53 0.9411759795612471 4.695445574307774E-4 0.0 3.1929290763380322 1.304290437307715E-4 54-55 0.9628989367946071 4.825874618038545E-4 0.0 3.5769317454379834 1.4347194810384865E-4 56-57 1.0042058149441424 5.21716174923086E-4 0.0 3.9820443552657596 1.4347194810384865E-4 58-59 1.019139940451316 5.478019836692402E-4 0.0 4.404993136823719 1.4347194810384865E-4 60-61 1.0311915840920391 5.478019836692402E-4 0.0 4.782089588058126 1.4347194810384865E-4 62-63 1.0415150429033297 5.608448880423174E-4 0.0 5.168022607005291 1.4347194810384865E-4 64-65 1.0497125083018086 6.130165055346261E-4 0.0 5.585982477640549 1.4347194810384865E-4 66-67 1.0608185413754838 6.391023142807803E-4 0.0 6.032095457365093 1.4347194810384865E-4 68-69 1.0735614589479803 6.782310274000118E-4 0.0 6.577458417916568 1.4999340029038722E-4 70-71 1.090002039910244 6.91273931773089E-4 0.0 7.222938712435783 1.565148524769258E-4 72-73 1.1281655781058677 7.173597405192432E-4 0.0 7.755173989735756 1.6955775685000295E-4 74-75 1.1657747928656357 7.238811927057819E-4 0.0 8.27629019105768 1.826006612230801E-4 76-77 1.1782242450897378 7.36924097078859E-4 0.0 8.83447432660789 1.826006612230801E-4 78-79 1.187406449768384 7.564884536384747E-4 0.0 9.373615821773408 1.8912211340961867E-4 80-81 1.2043557040011978 7.695313580115519E-4 0.0 9.985249779444487 2.021650177826958E-4 82-83 1.2104271759868652 7.695313580115519E-4 0.0 10.701585651970444 2.086864699692344E-4 84-85 1.2339044038584042 7.760528101980904E-4 0.0 11.36612815123091 2.086864699692344E-4 86-87 1.271683176375022 8.086600711307833E-4 0.0 12.029392445862818 2.086864699692344E-4 88 1.281139282045503 8.347458798769376E-4 0.0 12.56160815880623 2.086864699692344E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 2165 0.0 32.131485 3 CGATCTC 2295 0.0 28.051937 5 CCGATCT 2780 0.0 25.187408 4 TCTCGCG 1270 0.0 25.161627 8 AGTACTC 3345 0.0 23.320452 5 GATCTAC 2935 0.0 22.5937 1 CTCGCGC 1450 0.0 22.038113 9 TACACTC 3895 0.0 20.389389 5 TCTACAC 4430 0.0 19.734957 3 AGTACTT 23820 0.0 17.558271 12-13 ATCTCGC 2965 0.0 17.43395 7 AAGTACT 4605 0.0 17.246426 4 AAAGTAC 4485 0.0 16.872925 3 CGCGCCA 1620 0.0 16.547646 1 ATCTACA 4380 0.0 15.99884 2 GAGTACT 23085 0.0 15.786484 12-13 GTATAAG 2395 0.0 15.709443 1 GTACTAG 1650 0.0 15.676715 1 TATATAC 2495 0.0 15.44793 3 TCTAACG 870 0.0 15.13614 2 >>END_MODULE