##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765373_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5190402 Sequences flagged as poor quality 0 Sequence length 99 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.267301068395085 33.0 33.0 33.0 33.0 33.0 2 32.08609101953952 33.0 33.0 33.0 27.0 33.0 3 32.151896519768606 33.0 33.0 33.0 27.0 33.0 4 36.045525182827845 37.0 37.0 37.0 33.0 37.0 5 36.08532055898561 37.0 37.0 37.0 33.0 37.0 6 36.13114514058834 37.0 37.0 37.0 33.0 37.0 7 36.035375101967055 37.0 37.0 37.0 33.0 37.0 8 36.01286778943134 37.0 37.0 37.0 33.0 37.0 9 35.714958494544355 37.0 37.0 37.0 33.0 37.0 10-11 35.89850015470863 37.0 37.0 37.0 33.0 37.0 12-13 35.82358273983402 37.0 37.0 37.0 33.0 37.0 14-15 37.10216058409348 37.0 37.0 40.0 33.0 40.0 16-17 37.26000346408621 37.0 37.0 40.0 33.0 40.0 18-19 37.38452628524727 37.0 37.0 40.0 33.0 40.0 20-21 37.42876563318217 37.0 37.0 40.0 33.0 40.0 22-23 37.362226663753596 37.0 37.0 40.0 33.0 40.0 24-25 37.29908983928412 37.0 37.0 40.0 33.0 40.0 26-27 37.11426080292047 37.0 37.0 40.0 33.0 40.0 28-29 37.011644570112296 37.0 37.0 40.0 33.0 40.0 30-31 36.77924128034785 37.0 37.0 40.0 33.0 40.0 32-33 36.44674497274007 37.0 37.0 40.0 33.0 40.0 34-35 36.28559695376197 37.0 37.0 40.0 33.0 40.0 36-37 36.05697400702296 37.0 37.0 40.0 33.0 40.0 38-39 35.811761208476725 37.0 37.0 40.0 30.0 40.0 40-41 35.53524726986464 37.0 37.0 40.0 27.0 40.0 42-43 35.2238901726687 37.0 35.0 40.0 27.0 40.0 44-45 34.776196429486575 37.0 33.0 40.0 24.5 40.0 46-47 34.23468153333788 37.0 33.0 40.0 22.0 40.0 48-49 33.738895079803065 37.0 33.0 40.0 18.5 40.0 50-51 33.32594373229665 37.0 33.0 40.0 15.0 40.0 52-53 33.146831016171774 37.0 33.0 40.0 15.0 40.0 54-55 33.02888283027018 37.0 33.0 40.0 15.0 40.0 56-57 32.83270467297138 37.0 33.0 37.0 15.0 40.0 58-59 32.60867963598966 37.0 33.0 37.0 15.0 40.0 60-61 32.38558997935035 37.0 33.0 37.0 15.0 40.0 62-63 32.0031808711541 37.0 33.0 37.0 15.0 40.0 64-65 31.888416446356178 37.0 33.0 37.0 15.0 40.0 66-67 31.72668051530498 37.0 33.0 37.0 15.0 40.0 68-69 31.48275345532003 37.0 33.0 37.0 15.0 37.0 70-71 31.282334489698485 33.0 33.0 37.0 15.0 37.0 72-73 31.05763031456908 33.0 33.0 37.0 15.0 37.0 74-75 30.852574424871136 33.0 27.0 37.0 15.0 37.0 76-77 30.680188548016126 33.0 27.0 37.0 15.0 37.0 78-79 30.483740951086254 33.0 27.0 37.0 15.0 37.0 80-81 30.407595789304953 33.0 27.0 37.0 15.0 37.0 82-83 30.268810681715983 33.0 27.0 37.0 15.0 37.0 84-85 30.111139079400786 33.0 27.0 37.0 15.0 37.0 86-87 30.010115016139405 33.0 27.0 37.0 15.0 37.0 88-89 29.921997274970224 33.0 27.0 37.0 15.0 37.0 90-91 29.84151795949524 33.0 27.0 37.0 15.0 37.0 92-93 29.678319039642787 33.0 27.0 37.0 15.0 37.0 94-95 29.651855386153134 33.0 27.0 37.0 15.0 37.0 96-97 29.625478527482073 33.0 27.0 37.0 15.0 37.0 98-99 28.811653028031355 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15883501473170725 1101 2 0.37328680003835046 1101 3 0.2772467965526175 1101 4 0.22990534241725413 1101 5 0.08857075866248465 1101 6 0.13159406743577762 1101 7 0.21932897973285037 1101 8 0.16949859708376636 1101 9 0.44559378880847333 1101 10-11 0.35003571727073535 1101 12-13 0.3166086225032885 1101 14-15 0.6384563616176777 1101 16-17 0.49866204720772345 1101 18-19 0.3299279334416454 1101 20-21 0.3855948893097718 1101 22-23 0.32530613091935834 1101 24-25 0.3895353649499498 1101 26-27 0.283215634775587 1101 28-29 0.2446080981034342 1101 30-31 0.29135187055457834 1101 32-33 0.4226561946836469 1101 34-35 0.5978834230387093 1101 36-37 0.5780779706576382 1101 38-39 0.3964638756783927 1101 40-41 0.43085551096618957 1101 42-43 0.5841996862415044 1101 44-45 0.6514013738981603 1101 46-47 0.7025539063258606 1101 48-49 0.7380113067043936 1101 50-51 0.5738523628017091 1101 52-53 0.6279312311839078 1101 54-55 0.6997154090988715 1101 56-57 0.7412357437438999 1101 58-59 0.711082698169875 1101 60-61 0.5915670261414547 1101 62-63 0.5973699185307879 1101 64-65 0.5523818052963314 1101 66-67 0.6182441485369381 1101 68-69 0.5376259088678488 1101 70-71 0.6157993009115685 1101 72-73 0.7242160248415708 1101 74-75 0.5768580443866433 1101 76-77 0.5998302459131004 1101 78-79 0.7798035164245753 1101 80-81 0.7739626398055321 1101 82-83 0.6910860718006901 1101 84-85 0.7235972118020655 1101 86-87 0.5563078794934384 1101 88-89 0.5405460127687043 1101 90-91 0.6036972843490709 1101 92-93 0.7545900019649423 1101 94-95 0.782484954504497 1101 96-97 0.5985571645473478 1101 98-99 0.6754950164390152 1102 1 0.1016406744165792 1102 2 0.35502958084730096 1102 3 0.2783921776311473 1102 4 0.23372895969087182 1102 5 0.10685184771936918 1102 6 0.23462882444364652 1102 7 0.20667984428759922 1102 8 0.1486564173112086 1102 9 0.44576932078676634 1102 10-11 0.3020943954227562 1102 12-13 0.3208779761532625 1102 14-15 0.6257674171959096 1102 16-17 0.42373865116338294 1102 18-19 0.34944781887125487 1102 20-21 0.39176835364241924 1102 22-23 0.24449732301956573 1102 24-25 0.36389427125939733 1102 26-27 0.3061948193834638 1102 28-29 0.276953846001895 1102 30-31 0.3318821267424994 1102 32-33 0.36077271389724785 1102 34-35 0.4431529153760181 1102 36-37 0.46422144214690064 1102 38-39 0.41304197862437064 1102 40-41 0.3247807915193661 1102 42-43 0.36973705869137063 1102 44-45 0.4186422769029434 1102 46-47 0.601811199640764 1102 48-49 0.6186327221893038 1102 50-51 0.5073530583491106 1102 52-53 0.6496492084396834 1102 54-55 0.5534021021173743 1102 56-57 0.7150276708994028 1102 58-59 0.6444156062533182 1102 60-61 0.4935545013217606 1102 62-63 0.522630606022652 1102 64-65 0.4680287258415241 1102 66-67 0.5498594040444473 1102 68-69 0.5220651774728147 1102 70-71 0.4750015935857945 1102 72-73 0.6185448271839356 1102 74-75 0.5329500935423006 1102 76-77 0.44454711516012324 1102 78-79 0.6888862704247813 1102 80-81 0.5334020698192106 1102 82-83 0.577472851408757 1102 84-85 0.5227538791987953 1102 86-87 0.5368143005804704 1102 88-89 0.5697855896146464 1102 90-91 0.5058966705489745 1102 92-93 0.5388472325787639 1102 94-95 0.5744638694356503 1102 96-97 0.5464657607782328 1102 98-99 0.4860591672156147 1103 1 0.08505693390858227 1103 2 0.26439937466196994 1103 3 0.2705093227717299 1103 4 0.21431296116345777 1103 5 0.11936896769550742 1103 6 0.22552321067590242 1103 7 0.2017302314239089 1103 8 0.1812447433398674 1103 9 0.45369169314129465 1103 10-11 0.28706877662212094 1103 12-13 0.30537798430840013 1103 14-15 0.5004559256667775 1103 16-17 0.4698427527345288 1103 18-19 0.23646524990786588 1103 20-21 0.3200019262709617 1103 22-23 0.27904148545138696 1103 24-25 0.3549266425188051 1103 26-27 0.20548196664438478 1103 28-29 0.1299332461095375 1103 30-31 0.1830582382303021 1103 32-33 0.23655021362346673 1103 34-35 0.3820113117952175 1103 36-37 0.36042065599107076 1103 38-39 0.28361561141457514 1103 40-41 0.1897586910806197 1103 42-43 0.34409458484025635 1103 44-45 0.37121627317954164 1103 46-47 0.4828703430710988 1103 48-49 0.4544463841516091 1103 50-51 0.474703428942135 1103 52-53 0.4592589731754515 1103 54-55 0.43013188959433535 1103 56-57 0.44423028427336675 1103 58-59 0.44139268098005857 1103 60-61 0.4190212900825756 1103 62-63 0.4887915838942618 1103 64-65 0.2832258822595044 1103 66-67 0.34134599349355454 1103 68-69 0.3372670638739983 1103 70-71 0.3203244790153512 1103 72-73 0.30819907240210753 1103 74-75 0.38265110268305236 1103 76-77 0.30125991663829765 1103 78-79 0.5556743322267899 1103 80-81 0.38009425043467004 1103 82-83 0.4065647412351012 1103 84-85 0.473794638605753 1103 86-87 0.4260195002999332 1103 88-89 0.3962708625905975 1103 90-91 0.279785811858968 1103 92-93 0.24524645714112125 1103 94-95 0.4657221381685943 1103 96-97 0.4321379659523217 1103 98-99 0.38626036625470306 1104 1 0.1326112277329088 1104 2 0.34797451224551423 1104 3 0.2682983360141833 1104 4 0.22273925428845587 1104 5 0.09282310963111229 1104 6 0.22706910882151732 1104 7 0.17857179343088347 1104 8 0.12388261413406099 1104 9 0.4515777716371119 1104 10-11 0.2738624740226854 1104 12-13 0.2569212141902071 1104 14-15 0.4439336544436756 1104 16-17 0.3267110279176535 1104 18-19 0.20121307989174397 1104 20-21 0.3366421202487757 1104 22-23 0.2766481864199335 1104 24-25 0.3605178194598082 1104 26-27 0.1918636749516125 1104 28-29 0.19118806301282376 1104 30-31 0.2092939211245124 1104 32-33 0.20398663813470108 1104 34-35 0.264358231133869 1104 36-37 0.21473478049706785 1104 38-39 0.06854265405831939 1104 40-41 0.16373935513857418 1104 42-43 0.1738036142984214 1104 44-45 0.24170229800469656 1104 46-47 0.36503253762836607 1104 48-49 0.4578372550248204 1104 50-51 0.3613601096601258 1104 52-53 0.32440117178664707 1104 54-55 0.3892947494997969 1104 56-57 0.43125056651383886 1104 58-59 0.34516724742536553 1104 60-61 0.3108794285353156 1104 62-63 0.3271037829749872 1104 64-65 0.22855840175328623 1104 66-67 0.2451174755938581 1104 68-69 0.2917349170209995 1104 70-71 0.32050850142113063 1104 72-73 0.371662058926038 1104 74-75 0.41369318757812223 1104 76-77 0.24315072700796136 1104 78-79 0.40830028172216615 1104 80-81 0.40706788577423936 1104 82-83 0.3557384154343666 1104 84-85 0.41805637887107494 1104 86-87 0.4245188366695132 1104 88-89 0.38188622568251773 1104 90-91 0.3715173013036761 1104 92-93 0.4060275217058553 1104 94-95 0.4132425041978962 1104 96-97 0.34798939213741775 1104 98-99 0.314263737657555 1105 1 0.15645229149598094 1105 2 0.33924282435125264 1105 3 0.23061829944909107 1105 4 0.21179848097959564 1105 5 -0.0015866668486808067 1105 6 0.09278860405390787 1105 7 0.1608208689666526 1105 8 0.033216482505352474 1105 9 0.37022448097694394 1105 10-11 0.22804894474045057 1105 12-13 0.269006972166018 1105 14-15 0.43761631227597064 1105 16-17 0.41821972050762923 1105 18-19 0.25029694114266476 1105 20-21 0.3270796128509019 1105 22-23 0.21138398489659949 1105 24-25 0.31511848047895796 1105 26-27 0.27212616531795675 1105 28-29 0.20315921530089298 1105 30-31 0.2589145444338783 1105 32-33 0.3121336558029455 1105 34-35 0.3997363545465973 1105 36-37 0.3465803108163499 1105 38-39 0.3012193300747583 1105 40-41 0.23453279406941618 1105 42-43 0.32344511701532497 1105 44-45 0.26807459351660157 1105 46-47 0.40392136292294367 1105 48-49 0.3972140477490882 1105 50-51 0.3303659007131685 1105 52-53 0.2967361078075612 1105 54-55 0.3213382657128392 1105 56-57 0.3748045772341868 1105 58-59 0.32031217476681206 1105 60-61 0.28501083778175484 1105 62-63 0.2552799348618251 1105 64-65 0.18457052944827623 1105 66-67 0.24339522650261358 1105 68-69 0.2294044564964146 1105 70-71 0.24742729043240885 1105 72-73 0.33125246191310254 1105 74-75 0.24077011854502572 1105 76-77 0.26021802680825346 1105 78-79 0.26904098361276496 1105 80-81 0.278198592224598 1105 82-83 0.22643523160711254 1105 84-85 0.21172326011068066 1105 86-87 0.24658338088938336 1105 88-89 0.19677092517233064 1105 90-91 0.11553651235201912 1105 92-93 0.18232547982181657 1105 94-95 0.40013471840183 1105 96-97 0.2191627076203133 1105 98-99 0.15506932457987688 1106 1 0.08077934438527734 1106 2 0.26220071001539935 1106 3 0.1705162014718411 1106 4 0.14064147215391642 1106 5 0.059126258754993444 1106 6 0.15207829887174995 1106 7 0.1706070832237998 1106 8 0.06678176864943453 1106 9 0.4002360243022238 1106 10-11 0.25517603853683113 1106 12-13 0.2314341415275507 1106 14-15 0.46163981790403597 1106 16-17 0.3270786299272146 1106 18-19 0.26823917963442767 1106 20-21 0.3246337879365697 1106 22-23 0.17163654568963693 1106 24-25 0.283076329446331 1106 26-27 0.1548584059894722 1106 28-29 0.14339980177032174 1106 30-31 0.14661777849492807 1106 32-33 0.17629395341469234 1106 34-35 0.29640087296697715 1106 36-37 0.26086883269655914 1106 38-39 0.19052525422986122 1106 40-41 0.15908306301751196 1106 42-43 0.2033343090662214 1106 44-45 0.27147806077886827 1106 46-47 0.33397016782075184 1106 48-49 0.2987268518879489 1106 50-51 0.11623083628676767 1106 52-53 0.17252027483775123 1106 54-55 0.2617368521832901 1106 56-57 0.2965856970125529 1106 58-59 0.25326674503595825 1106 60-61 0.22602463132908213 1106 62-63 0.29138512104629655 1106 64-65 0.1123348997314828 1106 66-67 0.1820737309547198 1106 68-69 0.20608364879406693 1106 70-71 0.18418942287683393 1106 72-73 0.3740514719881638 1106 74-75 0.3526099869899575 1106 76-77 0.17269367517607392 1106 78-79 0.316828648343904 1106 80-81 0.26701152013277607 1106 82-83 0.20563426153165665 1106 84-85 0.20656268937224098 1106 86-87 0.11787749369683453 1106 88-89 0.17878611540191613 1106 90-91 -0.032661108204962375 1106 92-93 -0.024005562475004183 1106 94-95 0.26485382633978816 1106 96-97 0.20756542448639692 1106 98-99 0.17647425107502812 1107 1 0.10726641043452645 1107 2 0.2060685444977466 1107 3 0.19632385399899732 1107 4 0.19910653944841528 1107 5 0.07702872183707399 1107 6 0.11046554332723701 1107 7 0.06143208036851888 1107 8 0.06526258479574665 1107 9 0.32637087754267924 1107 10-11 0.16435549244535963 1107 12-13 0.10865246955292918 1107 14-15 0.3021849448590004 1107 16-17 0.2526954964912491 1107 18-19 0.12956583574389668 1107 20-21 0.22423634380712798 1107 22-23 0.09512879598982238 1107 24-25 0.17771096002266518 1107 26-27 0.00452902433440272 1107 28-29 0.08090007392362253 1107 30-31 0.036470350546082386 1107 32-33 -0.058612001180556206 1107 34-35 0.08255112729747793 1107 36-37 0.06784821049875944 1107 38-39 0.060881432860213636 1107 40-41 0.01492935053816069 1107 42-43 0.07211708124608407 1107 44-45 -0.007571210597092204 1107 46-47 0.03318037709530586 1107 48-49 0.17425164704104645 1107 50-51 0.024658069628742396 1107 52-53 0.051282308379072106 1107 54-55 0.10123753466574215 1107 56-57 0.13860829870295532 1107 58-59 0.11598417322779397 1107 60-61 0.1522053400779484 1107 62-63 0.1351445326274714 1107 64-65 -0.0297294630928171 1107 66-67 -0.05771636658873902 1107 68-69 -0.012969556450979525 1107 70-71 -0.06420589812249844 1107 72-73 0.03831421818101077 1107 74-75 -0.01610252691215308 1107 76-77 0.08448290272067993 1107 78-79 0.1438790215598864 1107 80-81 0.09346510296683874 1107 82-83 -0.06244553403793418 1107 84-85 -0.007637589743723083 1107 86-87 0.08380824794081221 1107 88-89 0.013726660402546997 1107 90-91 -0.11299720210776698 1107 92-93 -0.08680903197812029 1107 94-95 0.1529969710230219 1107 96-97 0.08785798129249756 1107 98-99 -0.07540997487529211 1108 1 0.11504434508219674 1108 2 0.27495783003175944 1108 3 0.15046069608116142 1108 4 0.14245481281071193 1108 5 -0.003056880359231684 1108 6 0.07807701381671706 1108 7 0.08805771377137006 1108 8 0.055198950074696995 1108 9 0.3508616498131829 1108 10-11 0.24776796379729404 1108 12-13 0.16664265404899936 1108 14-15 0.211737468192581 1108 16-17 0.27078695229185 1108 18-19 0.006299002242435847 1108 20-21 0.18655905135880602 1108 22-23 0.10857573645379404 1108 24-25 0.1454914110910721 1108 26-27 0.049916878654116204 1108 28-29 0.07290462250040974 1108 30-31 0.08123729267791191 1108 32-33 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18-19 -0.8234149182307249 2216 20-21 -1.0142988402737672 2216 22-23 -1.069473563004685 2216 24-25 -0.8867051900594163 2216 26-27 -1.1122188406753892 2216 28-29 -1.1546028599263352 2216 30-31 -0.8091184345526301 2216 32-33 -0.8918978027488791 2216 34-35 -0.9142353849722582 2216 36-37 -0.8821923486487151 2216 38-39 -1.009034701085838 2216 40-41 -1.108886305082109 2216 42-43 -1.1307166747837556 2216 44-45 -0.9812796395774654 2216 46-47 -1.2979244065584936 2216 48-49 -1.5058323664700737 2216 50-51 -1.0664761902917377 2216 52-53 -1.000698096164193 2216 54-55 -0.9821751853940768 2216 56-57 -1.0666198752557179 2216 58-59 -1.0392417214643608 2216 60-61 -1.0854394470625763 2216 62-63 -1.129169872008319 2216 64-65 -1.2263890273796356 2216 66-67 -1.1363941864100653 2216 68-69 -1.1347589230702013 2216 70-71 -1.1339247343770396 2216 72-73 -1.0522502840924837 2216 74-75 -1.051517677403151 2216 76-77 -0.9362454042443638 2216 78-79 -0.9477545367593798 2216 80-81 -0.9068933443445673 2216 82-83 -0.9144215991273796 2216 84-85 -0.921400062197673 2216 86-87 -1.0884775058513831 2216 88-89 -1.0215284021601576 2216 90-91 -0.9274225914347163 2216 92-93 -0.8781027057369712 2216 94-95 -1.0496978382234374 2216 96-97 -1.039660096324404 2216 98-99 -1.046735209523888 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 32.0 10 108.0 11 312.0 12 525.0 13 960.0 14 2818.0 15 7645.0 16 17328.0 17 32543.0 18 51011.0 19 67267.0 20 82015.0 21 100119.0 22 110911.0 23 89542.0 24 57823.0 25 44439.0 26 49326.0 27 61706.0 28 81064.0 29 105870.0 30 137500.0 31 180032.0 32 234731.0 33 309288.0 34 407879.0 35 566361.0 36 886932.0 37 1163857.0 38 340057.0 39 395.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37083529563037 20.395005333142613 13.732866327522622 22.501293043704397 2 13.984735160114727 22.72743212575959 38.525705178030066 24.762127536095612 3 17.79305340896524 30.588420704215203 30.449780190436115 21.16874569638344 4 12.183931032702285 19.090236170531686 37.51248939870168 31.213343398064353 5 11.022430850429464 39.808993957390186 34.016908826395046 15.1516663657853 6 27.288290964746082 38.697965976431114 18.333011585615143 15.680731473207663 7 21.742208021652274 34.664656032422926 24.54728940070538 19.045846545219426 8 27.482765304113244 31.8731959489843 20.74910575327306 19.894932993629393 9 27.438352508913443 12.921942239164425 21.702248181426707 37.93745707049542 10-11 24.454165592568742 26.692065470073416 26.376723806749457 22.47704513060838 12-13 27.35170609135863 24.75566439747827 27.11018337307977 20.782446138083333 14-15 24.827443765149155 22.829809540534978 28.359941964422042 23.98280472989382 16-17 20.573094135325253 25.632738536662625 30.41433847053771 23.379828857474415 18-19 21.363903990480893 24.07655514929287 34.98078761529454 19.5787532449317 20-21 22.177887184846185 23.387639338918255 34.338014666301376 20.09645880993418 22-23 21.85587937119321 23.574291548130567 32.23495983548095 22.334869245195264 24-25 21.306943084562622 24.475869113798893 32.36407314886207 21.853114652776412 26-27 22.209618831065495 22.853567026214925 32.52335753569762 22.41345660702196 28-29 20.952558202620914 22.884682149860456 34.291582809963465 21.871176837555165 30-31 22.417059410812495 23.111938150455398 33.47442067107711 20.99658176765499 32-33 20.77247581208546 23.94155597196518 33.09079913270687 22.19516908324249 34-35 20.426394718559372 24.582951378332545 33.950144902071166 21.040509001036913 36-37 22.434418374530527 23.232487579960086 32.318903237167376 22.01419080834201 38-39 22.923860232791217 22.711709035253914 32.187439431473706 22.17699130048116 40-41 21.525856764081087 23.101707728996715 32.7386973109212 22.633738196001 42-43 23.13518297812 23.12246720003576 31.127858689943476 22.614491131900767 44-45 23.340332184026742 23.388006957492095 30.631364664139937 22.640296194341218 46-47 22.536077167047946 23.651694030635777 30.951793329302816 22.860435473013457 48-49 24.15055712447706 24.29730876336746 29.182238678237255 22.36989543391822 50-51 22.183888646775337 25.442682474305457 27.942488847684633 24.430940031234574 52-53 21.37297835713852 26.166871692809508 26.25732723617374 26.20282271387823 54-55 21.757043096083887 26.361869465987414 25.886164501323787 25.994922936604908 56-57 23.530268557841826 24.345347994635432 26.038489096984346 26.085894350538393 58-59 21.899652473931692 24.584425252610494 28.461841683938932 25.054080589518886 60-61 22.88243762236528 25.197836314027317 27.235925078635525 24.683800984971878 62-63 21.701575330773995 24.91307995026204 27.249430776267424 26.135913942696536 64-65 20.350437210836464 25.575475656798837 28.08135092426367 25.99273620810103 66-67 23.345889200875 24.38664673757447 27.206380160920098 25.061083900630432 68-69 22.757644866504197 23.739984467218065 27.67878353386395 25.82358713241379 70-71 21.801163130211492 24.568999191871978 27.46094922915723 26.168888448759297 72-73 23.49234221164372 23.80638339766361 27.766702848835216 24.93457154185745 74-75 23.674322335726597 23.957306197092247 27.68562049721775 24.682750969963404 76-77 22.2172868305769 24.691074024709454 28.16607461233253 24.925564532381113 78-79 22.65515241658607 25.18172079886251 27.600281674944803 24.562845109606616 80-81 21.444764932173207 25.589463760942653 27.910799130617896 25.054972176266237 82-83 21.735146911549432 25.022069581508333 27.039100246955822 26.203683259986416 84-85 22.207287605083383 25.63410310029936 25.912289645387776 26.246319649229484 86-87 21.856081667662735 24.586322986157914 26.905411180097417 26.652184166081934 88-89 22.392138412400428 23.490598608739745 27.376819753075004 26.740443225784823 90-91 22.540362857510846 24.36490976728217 26.854725309849396 26.240002065357587 92-93 21.78193986582969 24.45721559661706 27.08983519106202 26.671009346491232 94-95 20.3817607643076 24.866681013746415 28.11958913919641 26.631969082749574 96-97 22.30385392375961 24.369750570550107 26.66328926820561 26.663106237484673 98-99 22.4186970488991 24.530672190708927 26.41923496484473 26.631395795547242 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 185.0 1 175.5 2 338.5 3 519.5 4 361.5 5 313.5 6 653.0 7 1738.0 8 2987.0 9 2731.5 10 1468.5 11 772.5 12 752.5 13 943.5 14 1219.0 15 1850.5 16 2583.5 17 3378.5 18 4530.5 19 5818.0 20 7549.0 21 9629.5 22 11913.0 23 14841.0 24 18293.5 25 22398.5 26 26615.5 27 30677.5 28 36876.5 29 46450.5 30 54367.5 31 61926.5 32 70572.0 33 75532.5 34 79619.5 35 86394.0 36 88330.5 37 96729.5 38 114248.0 39 108490.0 40 97610.0 41 102246.0 42 107576.5 43 119784.5 44 149639.0 45 196153.5 46 226118.5 47 259693.5 48 294968.0 49 357929.0 50 447432.5 51 377859.5 52 248198.0 53 210743.5 54 174905.5 55 152667.0 56 133942.0 57 95002.5 58 85229.5 59 72080.5 60 46332.0 61 34966.0 62 25718.0 63 18744.5 64 10984.0 65 5033.0 66 3942.5 67 3310.0 68 2919.0 69 2413.0 70 2034.5 71 2018.5 72 2084.5 73 1886.5 74 1532.5 75 1629.0 76 2451.5 77 2765.0 78 1866.0 79 1516.5 80 2032.5 81 2042.5 82 2222.0 83 2051.0 84 1373.0 85 1018.5 86 472.5 87 159.0 88 127.0 89 104.5 90 76.0 91 50.5 92 29.0 93 20.5 94 13.0 95 2.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.058357714874493344 2 0.008400120067771244 3 0.0 4 0.0 5 0.001830301390913459 6 0.0 7 0.0 8 0.0 9 0.003988130399148275 10-11 0.0 12-13 0.0 14-15 0.0026491204342168487 16-17 0.004334924346900298 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 5.009245911973678E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.3486431301467592E-4 54-55 0.0 56-57 9.440501911027315E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0017147034083294513 70-71 2.2156279995268189E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.238592694746958E-4 80-81 9.440501911027315E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.3716078253668983E-4 92-93 0.0011270803301940775 94-95 5.972562433507076E-4 96-97 3.178944521060218E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 5190402.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.727564092602913 #Duplication Level Percentage of deduplicated Percentage of total 1 73.35266729621938 11.536587762646679 2 11.95924896587129 3.761797092202716 3 4.7801283636229535 2.2553932562924723 4 2.4733990855522996 1.556021705784369 5 1.4527959129042138 1.142447041683629 6 0.9481341822326078 0.8947104671670594 7 0.6304377644084898 0.6940675242292272 8 0.4340826802901542 0.5461650540601808 9 0.35582999819316413 0.5036705192388391 >10 2.5432232226845057 8.134320610393134 >50 0.41072088296430176 4.541367489143433 >100 0.4837965497461229 16.557980394468107 >500 0.08984079681252458 10.00478542926552 >1k 0.07777825626147232 22.735489827100977 >5k 0.005528664419232281 5.784964530632481 >10k+ 0.002387377817395758 9.350231295691207 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 80963 1.5598599106581725 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 76910 1.481773473422675 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 32906 0.6339778691515608 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26867 0.517628499680757 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 24802 0.4778435273414275 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 24094 0.46420296539651457 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 22556 0.4345713491941472 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 22442 0.432374987525051 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 21856 0.4210849178926796 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19429 0.3743255339374484 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 16623 0.320264210748994 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 15084 0.29061332821619595 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 14762 0.28440956981752086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13901 0.26782125931671574 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 13860 0.26703133976905835 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 12411 0.23911442697502044 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 12385 0.23861350238382306 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 10932 0.21061952426806246 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 10361 0.19961844959215108 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 9989 0.19245137467194257 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 8936 0.17216392872844916 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 8890 0.17127767752863843 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8699 0.16759780841638086 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 8479 0.1633592157216339 No Hit ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT 8158 0.15717472365338947 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 8075 0.155575618227644 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8041 0.15492056299300133 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 8001 0.15414990977577459 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 7857 0.1513755581937584 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 7613 0.14667457356867541 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 7547 0.1454029957602513 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7350 0.14160752866540974 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 7238 0.13944969965717494 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7204 0.1387946444225322 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 7192 0.13856344845736418 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 7007 0.13499917732769062 No Hit CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA 6986 0.13459458438864658 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 6850 0.13197436345007574 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 6612 0.12738897680757674 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 6587 0.12690731854681006 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 6460 0.12446049458211522 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 6436 0.12399810265177919 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 6385 0.12301551979981512 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 6324 0.12184027364354438 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6301 0.121397148043639 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 6273 0.12085769079158032 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 5866 0.11301629430629843 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 5835 0.11241903806294773 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5763 0.11103186227193963 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 5596 0.10781438509001808 No Hit ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG 5507 0.10609968168168862 No Hit TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG 5502 0.10600335002953529 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 5456 0.10511709882972456 No Hit CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC 5444 0.10488590286455654 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 5323 0.10255467688244572 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 5216 0.10049317952636425 No Hit ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT 5216 0.10049317952636425 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.853266086133598E-5 0.0 6 0.0 0.0 0.0 3.853266086133598E-5 0.0 7 0.0 0.0 0.0 5.779899129200397E-5 0.0 8 1.926633043066799E-5 0.0 0.0 5.779899129200397E-5 0.0 9 1.926633043066799E-5 0.0 0.0 5.779899129200397E-5 0.0 10-11 3.853266086133598E-5 0.0 0.0 1.0596481736867394E-4 0.0 12-13 5.779899129200397E-5 0.0 0.0 1.637638086606779E-4 0.0 14-15 7.706532172267196E-5 0.0 0.0 2.6009546081401787E-4 0.0 16-17 7.706532172267196E-5 0.0 0.0 3.564271129673578E-4 0.0 18-19 7.706532172267196E-5 0.0 0.0 4.5275876512069775E-4 0.0 20-21 7.706532172267196E-5 0.0 0.0 9.922160171794015E-4 0.0 22-23 7.706532172267196E-5 0.0 0.0 0.004190426868670287 0.0 24-25 7.706532172267196E-5 0.0 0.0 0.011473099771462788 0.0 26-27 1.2523114779934195E-4 0.0 0.0 0.019439727404544003 0.0 28-29 1.3486431301467592E-4 0.0 0.0 0.045198811190347105 0.0 30-31 1.5413064344534393E-4 0.0 0.0 0.11840123366167013 0.0 32-33 1.5413064344534393E-4 0.0 0.0 0.22563762883876817 0.0 34-35 1.5413064344534393E-4 0.0 0.0 0.3530458719767756 0.0 36-37 1.733969738760119E-4 9.633165215333995E-6 0.0 0.5455165129791488 0.0 38-39 1.830301390913459E-4 1.926633043066799E-5 0.0 0.8473717450016396 0.0 40-41 2.1192963473734788E-4 1.926633043066799E-5 0.0 1.258351472583434 0.0 42-43 2.311959651680159E-4 1.926633043066799E-5 0.0 1.5772477738718504 0.0 44-45 2.504622955986839E-4 1.926633043066799E-5 0.0 1.8984656679771623 0.0 46-47 2.504622955986839E-4 1.926633043066799E-5 0.0 2.2279391846720156 0.0 48-49 2.504622955986839E-4 1.926633043066799E-5 0.0 2.5854066794826296 0.0 50-51 2.6972862602935184E-4 1.926633043066799E-5 0.0 3.031277731474364 0.0 52-53 2.8899495646001985E-4 1.926633043066799E-5 0.0 3.4734592811886245 0.0 54-55 2.986281216753538E-4 1.926633043066799E-5 0.0 3.840309478918974 0.0 56-57 3.0826128689068785E-4 1.926633043066799E-5 0.0 4.230654966609523 0.0 58-59 3.0826128689068785E-4 1.926633043066799E-5 0.0 4.671911732463112 0.0 60-61 3.0826128689068785E-4 1.926633043066799E-5 0.0 5.108351915709033 0.0 62-63 3.0826128689068785E-4 1.926633043066799E-5 0.0 5.563413007316196 0.0 64-65 3.0826128689068785E-4 1.926633043066799E-5 0.0 6.034831213458996 0.0 66-67 3.0826128689068785E-4 1.926633043066799E-5 0.0 6.507925590349264 0.0 68-69 3.0826128689068785E-4 1.926633043066799E-5 0.0 7.041805239748289 0.0 70-71 3.0826128689068785E-4 1.926633043066799E-5 0.0 7.712543267361564 0.0 72-73 3.0826128689068785E-4 1.926633043066799E-5 0.0 8.298827335532007 0.0 74-75 3.0826128689068785E-4 1.926633043066799E-5 0.0 8.849931084336049 0.0 76-77 3.2752761732135585E-4 1.926633043066799E-5 0.0 9.427516404317046 0.0 78-79 3.2752761732135585E-4 1.926633043066799E-5 0.0 9.98185689663344 0.0 80-81 3.2752761732135585E-4 1.926633043066799E-5 0.0 10.603562113300665 0.0 82-83 3.2752761732135585E-4 1.926633043066799E-5 0.0 11.326829790833157 0.0 84-85 3.2752761732135585E-4 1.926633043066799E-5 0.0 11.994254009612359 0.0 86-87 3.2752761732135585E-4 1.926633043066799E-5 0.0 12.627316728068461 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 490 0.0 50.300236 2 TCACGCA 560 0.0 47.33077 4 TAATAAT 2645 0.0 32.875523 3 TCAAGAC 1070 0.0 32.15916 3 TTAAGGT 990 0.0 31.93967 4 TTCTGGC 2810 0.0 31.27758 9 AGGGCTA 2300 0.0 30.32635 5 TATTAAG 1055 0.0 29.974134 2 AGGTGTT 1100 0.0 28.3227 7 TATTCGC 515 0.0 27.08892 9 CTATTGA 2640 0.0 26.77425 9 AGTACTC 840 0.0 26.57166 5 GTACTAG 4265 0.0 26.292952 1 ATTAAGG 1275 0.0 25.894342 3 TCAACGA 755 0.0 25.867773 3 CCGAGTA 580 0.0 25.65614 8 TTCAACG 855 0.0 24.475782 2 TAGTACT 6150 0.0 24.119722 4 CTAACTG 1835 0.0 24.07378 4 GGGCTAT 3025 0.0 23.980145 6 >>END_MODULE