FastQCFastQC Report
Fri 27 May 2016
ERR765372_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765372_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6859015
Sequences flagged as poor quality0
Sequence length99
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA108930.15881289077221727No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT99480.1450354023135975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA261800.019.999721
GTACTAG20350.018.7371961
GTACAAG36000.018.341761
CATGGGG156750.018.1843834
GTACATG397200.017.9936731
TACATGG391250.017.6845592
TAGTACT29300.017.4570454
TATATAC24200.017.2930983
GTATAAG24600.016.8232571
AACGCAG292300.016.7352646
ACATGGG384600.016.4669633
CAACGCA296200.016.248045
ATCAACG298250.016.1987233
GTATTAT16100.016.1739961
CTAGTAC30800.016.153953
TATCAAC324150.016.023332
ACGCAGA307800.015.4393137
TCAACGC314050.015.3689524
CGCAGAG308800.015.3140258
TAGGCAT30850.015.072685
Produced by FastQC (version 0.11.5)