FastQCFastQC Report
Fri 27 May 2016
ERR765372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765372_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6859015
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197210.28751941787559876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120680.17594363039007788No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT107960.15739869354418964No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA105170.1533310540945019No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103890.15146489692762008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA344700.031.4359251
TATCACG4750.031.3282322
ATCAACG433550.024.73433
CAACGCA436100.024.557685
AACGCAG457200.023.7375666
TCAACGC453300.023.7284534
TATCAAC458300.023.7232482
GGTATCA229200.022.8977341
AGTACTT263300.022.39646712-13
ACGCAGA491300.021.8628487
CGCAGAG497800.021.5960588
GCAGAGT529150.020.2468689
TACTTTT297350.019.83210614-15
GAGTACT250150.019.10260212-13
AGAGTAC509150.018.67867510-11
TCTAACG11050.018.516972
GTACTAG22650.018.2818371
ATCACGC9250.018.0984043
TAGTACT26600.018.006744
CTAACGC10950.017.8367083
Produced by FastQC (version 0.11.5)