FastQCFastQC Report
Fri 27 May 2016
ERR765359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11006516
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA860950.7822184604101788No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT717440.6518320602086982No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG398310.36188563211101493No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG323770.29416211269760567No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT311340.28286880244393414No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG307300.279198249473312No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC253160.23000920545611345No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA227500.20669574277636993No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC225590.20496040708976393No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC223390.20296159111566273No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC210840.19155925453613115No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT209180.19005105702840028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208490.18942415565470488No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG129260.11743952400559814No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC114230.10378397669162522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG26350.023.0088671
GATCTAC57950.020.6810651
TCTACAC81300.019.4221523
CTACACT94500.019.0473844
TACACTC73500.018.9301345
GTATAAG37800.018.650291
CATGGGG229150.017.6789234
TCTAACG15750.017.3067632
CTAACGC16150.016.8773463
TGATCGC23900.016.1294869
GTATAAT30700.015.4621241
ATCTACA82550.015.31452752
GTCCTAC35900.015.186221
GTGATCG25550.015.0864858
ATTTAGG43700.014.6266091
ATAAGGT36650.014.6177173
GTATATA32250.014.4275181
GGGCTAT46500.014.1524726
TTTAGGG51150.014.0576962
CTAGGCA55100.013.9881624
Produced by FastQC (version 0.11.5)