##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765359_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11006516
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02059443696807	33.0	33.0	33.0	33.0	33.0
2	32.03935441514826	33.0	33.0	33.0	33.0	33.0
3	32.04855069487929	33.0	33.0	33.0	33.0	33.0
4	35.81584681292427	37.0	37.0	37.0	33.0	37.0
5	35.84988583126577	37.0	37.0	37.0	33.0	37.0
6	35.78528137332467	37.0	37.0	37.0	33.0	37.0
7	35.71883954922702	37.0	37.0	37.0	33.0	37.0
8	35.70095178165371	37.0	37.0	37.0	33.0	37.0
9	35.705814628352876	37.0	37.0	37.0	33.0	37.0
10-11	35.67166749223823	37.0	37.0	37.0	33.0	37.0
12-13	35.6622936813066	37.0	37.0	37.0	33.0	37.0
14-15	37.38339952442716	40.0	37.0	40.0	33.0	40.0
16-17	37.410913771442296	40.0	37.0	40.0	33.0	40.0
18-19	37.457169689300414	40.0	37.0	40.0	33.0	40.0
20-21	37.471856353091205	40.0	37.0	40.0	33.0	40.0
22-23	37.43836914424146	40.0	37.0	40.0	33.0	40.0
24-25	37.39530538092163	40.0	37.0	40.0	33.0	40.0
26-27	37.32714293969136	40.0	37.0	40.0	33.0	40.0
28-29	37.26977823863609	40.0	37.0	40.0	33.0	40.0
30-31	37.20697852980907	40.0	37.0	40.0	33.0	40.0
32-33	37.10407057964573	40.0	37.0	40.0	33.0	40.0
34-35	37.030007997081	40.0	37.0	40.0	33.0	40.0
36-37	36.938406167764626	40.0	37.0	40.0	33.0	40.0
38-39	36.849017572863204	40.0	37.0	40.0	33.0	40.0
40-41	36.740632503509744	40.0	37.0	40.0	33.0	40.0
42-43	36.61760488059982	38.5	37.0	40.0	33.0	40.0
44-45	36.430771690151545	37.0	37.0	40.0	33.0	40.0
46-47	36.21634589001643	37.0	37.0	40.0	33.0	40.0
48-49	35.987708917154166	37.0	37.0	40.0	33.0	40.0
50-51	35.787711933549176	37.0	37.0	40.0	33.0	40.0
52-53	35.612859873187844	37.0	37.0	40.0	33.0	40.0
54-55	35.451725368863315	37.0	37.0	40.0	27.0	40.0
56-57	35.26982507452858	37.0	37.0	40.0	27.0	40.0
58-59	35.04369502574657	37.0	33.0	40.0	27.0	40.0
60-61	34.80422719596283	37.0	33.0	40.0	27.0	40.0
62-63	34.524256176977346	37.0	33.0	38.5	27.0	40.0
64-65	34.27661091847774	37.0	33.0	37.0	27.0	40.0
66-67	34.05812007178294	37.0	33.0	37.0	27.0	40.0
68-69	33.786829047447895	37.0	33.0	37.0	27.0	40.0
70-71	33.48271505715341	37.0	33.0	37.0	27.0	40.0
72-73	33.19623993641585	37.0	33.0	37.0	27.0	37.0
74-75	32.95457104682354	37.0	33.0	37.0	27.0	37.0
76-77	32.67376743012957	37.0	33.0	37.0	27.0	37.0
78-79	32.45418986353175	37.0	33.0	37.0	27.0	37.0
80-81	32.264053039127006	37.0	33.0	37.0	27.0	37.0
82-83	32.09119825020015	33.0	33.0	37.0	24.5	37.0
84-85	31.90759264784606	33.0	33.0	37.0	22.0	37.0
86-87	31.71602508005258	33.0	33.0	37.0	22.0	37.0
88-89	31.510924755844627	33.0	33.0	37.0	22.0	37.0
90-91	31.32606966636854	33.0	33.0	37.0	22.0	37.0
92-93	31.12478408244716	33.0	33.0	37.0	22.0	37.0
94-95	30.904373327581588	33.0	33.0	37.0	15.0	37.0
96-97	30.687319720427425	33.0	33.0	37.0	15.0	37.0
98-99	30.375999453414686	33.0	33.0	37.0	8.5	37.0
100	30.092595513421323	33.0	33.0	37.0	2.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66197.0
3	16406.0
4	9972.0
5	8610.0
6	9932.0
7	14414.0
8	15726.0
9	14235.0
10	14804.0
11	16830.0
12	20313.0
13	24322.0
14	26625.0
15	31104.0
16	37034.0
17	43927.0
18	42739.0
19	32754.0
20	27473.0
21	27478.0
22	31904.0
23	40071.0
24	51138.0
25	62616.0
26	73529.0
27	86730.0
28	109298.0
29	142395.0
30	187585.0
31	253925.0
32	354203.0
33	508471.0
34	746921.0
35	1184723.0
36	2120356.0
37	3315541.0
38	1235389.0
39	826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.981068310764066	18.263988193726256	12.789402993401403	24.965540502108276
2	15.314426293174616	21.21527759793703	37.38466150306399	26.08563460582437
3	18.105400219983217	26.320960148145655	29.465975065842763	26.10766456602836
4	12.94079716424247	17.796163207267355	36.182233842404926	33.08080578608525
5	13.801330853067512	37.55246576368126	33.69205231819804	14.954151065053187
6	31.66281986644336	36.173556941108295	17.77157193586076	14.392051256587587
7	26.887734765527714	32.38745533061952	22.016009062576973	18.708800841275792
8	27.137845344912115	32.9349370480919	20.489520435004067	19.43769717199191
9	27.325916641633608	15.09073780381095	19.292466656962382	38.29087889759307
10-11	24.416271547327586	26.169412851708007	27.498697625958172	21.91561797500623
12-13	26.26469899265321	23.84704215569415	26.92783125582785	22.960427595824793
14-15	24.34289559083357	24.161796795175544	24.88178512838243	26.613522485608453
16-17	23.326686409503967	27.03293668806188	26.195443367204916	23.444933535229236
18-19	24.086215761387027	26.392809404579303	27.39175814023391	22.12921669379976
20-21	24.5431098351799	25.862166719456926	26.704421243382363	22.890302201980806
22-23	23.97982065632386	26.034053710061904	25.699964964271913	24.286160669342323
24-25	23.839733261319076	26.44782098108281	25.598638210857604	24.113807546740514
26-27	24.188723159111476	25.80565885238466	25.6131797056174	24.39243828288646
28-29	23.984879974679725	25.6124310314774	26.6634842175525	23.73920477629038
30-31	24.726147516984962	25.515041097576844	26.34762278677618	23.411188598662008
32-33	23.29842142294352	26.096347132859815	26.404836727247353	24.200394716949315
34-35	23.354873059170306	26.14075937130258	26.65462368170541	23.84974388782171
36-37	24.322031438263554	25.904838249755585	26.023823259288882	23.74930705269198
38-39	24.55744851504327	25.36517005017755	26.028677012780427	24.04870442199875
40-41	23.904060124512245	25.854837713906758	26.13917676507929	24.1019253965017
42-43	24.707605022334043	25.519210620327087	25.823984628741737	23.949199728597133
44-45	24.65501799116087	25.420941558618548	26.12120856409058	23.80283188613
46-47	24.10410917981851	25.50857434009694	26.779036902093246	23.6082795779913
48-49	24.552689248009845	25.618399223940386	26.488758643069595	23.34015288498018
50-51	23.790410076131447	25.989825151365135	26.024840493237	24.19492427926641
52-53	23.427822666680722	26.39809324444325	25.201741077156775	24.972343011719257
54-55	23.81185990707914	26.029331869543306	25.119683782096114	25.03912444128144
56-57	24.234951510665617	25.268135512747993	25.401605695697256	25.09530728088914
58-59	23.99115724118468	25.18248926523709	26.423883901638966	24.402469591939266
60-61	24.2433608973585	25.592545957372998	25.851660487394877	24.31243265787362
62-63	23.18902025544172	25.74540032791253	26.150083868328274	24.91549554831748
64-65	23.019936500932104	25.839759656432392	26.06581441164493	25.074489430990575
66-67	24.048676806516948	25.697928413157445	25.55544155839789	24.69795322192772
68-69	24.23540870103808	25.126629186487214	25.702212680329595	24.935749432145112
70-71	23.541820137063898	25.595924804643438	25.68893280655758	25.173322251735087
72-73	24.342407628323688	25.411889630849526	25.580265447380455	24.66543729344633
74-75	24.503536298475105	25.313443450052258	25.671709971138128	24.511310280334513
76-77	23.85037327221726	25.60156474390503	26.10977395225863	24.43828803161908
78-79	24.00244021810937	25.66474452768322	25.920779328642872	24.41203592556453
80-81	23.725018996205304	25.692096783536396	25.92097805753721	24.6619061627211
82-83	23.627014335903823	25.881775648386995	25.308562127361345	25.18264788834783
84-85	23.855424653932783	25.881588933664446	25.040214167791763	25.222772244611008
86-87	23.99033107301591	25.272813746258045	25.403936630663456	25.332918550062587
88-89	24.03179924812744	24.895667038227103	25.807759983136418	25.264773730509038
90-91	24.19146013809257	25.311679812389105	25.57210407627818	24.924755973240146
92-93	23.533873360116107	25.463930881152073	25.73098163633577	25.271214122396046
94-95	23.05028936036305	25.57020277738514	25.958654860623913	25.420853001627897
96-97	23.819775280715085	25.302763891533765	25.49784757727318	25.379613250477973
98-99	23.95920648543132	25.287360134181586	25.2784549198742	25.474978460512894
100	23.585376706612298	25.455309390307807	25.591635742674217	25.367678160405678
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4393.0
1	3793.5
2	3301.0
3	3261.0
4	2561.5
5	2164.0
6	2888.5
7	5363.0
8	8868.0
9	8423.5
10	4749.5
11	2937.0
12	2830.0
13	3011.5
14	3299.0
15	3779.0
16	4280.0
17	4927.0
18	5868.5
19	6937.0
20	8252.5
21	10111.5
22	12846.5
23	16170.0
24	19985.5
25	25315.0
26	32500.5
27	40280.0
28	48603.5
29	60139.5
30	72783.5
31	85662.0
32	100332.0
33	113778.0
34	128803.0
35	143049.5
36	158476.5
37	180065.5
38	199336.5
39	213572.0
40	223933.5
41	233676.0
42	248447.0
43	273219.0
44	303567.0
45	335415.5
46	372917.0
47	411691.0
48	479409.5
49	551878.5
50	659008.0
51	662061.5
52	533685.5
53	477992.0
54	456650.0
55	423889.5
56	388616.5
57	354386.0
58	331721.0
59	302817.0
60	254567.5
61	209276.5
62	169249.5
63	133944.5
64	102298.0
65	77165.0
66	59659.0
67	45746.0
68	35336.5
69	28435.0
70	23172.0
71	18607.0
72	14712.5
73	12774.5
74	10250.0
75	7888.5
76	6191.5
77	4513.0
78	3437.0
79	2510.5
80	1792.0
81	1302.0
82	944.0
83	739.5
84	559.0
85	354.5
86	223.5
87	131.5
88	70.0
89	48.0
90	44.5
91	29.0
92	11.5
93	10.0
94	10.0
95	7.5
96	3.0
97	1.5
98	1.5
99	4.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007650013864514439
2	0.012801507761402427
3	0.010275731212310962
4	0.01733518581175006
5	0.011302395780826558
6	0.0036251253348471035
7	0.003407072683126977
8	0.02254119287156808
9	0.00972151405585564
10-11	0.014654955301023504
12-13	0.010630066771356167
14-15	0.008367770509759854
16-17	0.010670951643553691
18-19	0.010870833240963806
20-21	0.00634169795419368
22-23	0.005524000510243206
24-25	0.01170215897564679
26-27	0.015331827074071394
28-29	0.0028846548717141735
30-31	8.040691532179666E-4
32-33	0.0013446580189407802
34-35	9.857796963180718E-4
36-37	7.949836260629612E-4
38-39	0.0
40-41	0.002630260111374026
42-43	0.0
44-45	0.0
46-47	1.1811185301506854E-4
48-49	0.006000990685880982
50-51	0.012174606387707064
52-53	0.01875252804793088
54-55	0.020038130140364128
56-57	0.03347108203903942
58-59	0.039008710840015136
60-61	0.03149497988282578
62-63	0.02146001514012245
64-65	0.019102320843398583
66-67	0.021287390124177352
68-69	0.017662264789330247
70-71	0.0189569524089185
72-73	0.020565090715354433
74-75	0.016894537744732305
76-77	0.009167296899400318
78-79	0.016008698847119286
80-81	0.009344464678922922
82-83	0.016385748224052005
84-85	0.023808623909691316
86-87	0.014282448687668288
88-89	0.026552453110502906
90-91	0.030136693573152483
92-93	0.02546218985190227
94-95	0.016467517968447053
96-97	0.022900071194190786
98-99	0.015695248160271607
100	0.01187478399159189
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1006516E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.30208760576191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.62128465655455	26.363042376452167
2	13.635562103347688	9.899987400590698
3	4.893381955234409	5.329199412821221
4	2.384646542324935	3.462705907530281
5	1.3830265797002974	2.5103376028688733
6	0.9032544719707029	1.9674013783066004
7	0.6365166258523092	1.6174817619950155
8	0.4683469557699176	1.360157777460116
9	0.37126209587947073	1.2129830216383795
>10	2.311608655308439	15.906857354349796
>50	0.2156782055013838	5.433408554182109
>100	0.1513738286497194	10.995044275155532
>500	0.01617534179117717	3.981192514769552
>1k	0.006931140488055153	4.11655439731912
>5k	5.254651096790774E-4	1.3666128369148247
>10k+	4.2537651735925303E-4	4.477033427645803
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	86095	0.7822184604101788	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	71744	0.6518320602086982	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	39831	0.36188563211101493	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	32377	0.29416211269760567	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	31134	0.28286880244393414	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	30730	0.279198249473312	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	25316	0.23000920545611345	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	22750	0.20669574277636993	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	22559	0.20496040708976393	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	22339	0.20296159111566273	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	21084	0.19155925453613115	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	20918	0.19005105702840028	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20849	0.18942415565470488	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	12926	0.11743952400559814	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	11423	0.10378397669162522	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.179934504251845E-4	0.0	0.0	2.7256581465015813E-5	0.0
2	4.724474120602741E-4	9.085527155005271E-6	0.0	5.451316293003163E-5	0.0
3	4.997039935252899E-4	9.085527155005271E-6	0.0	5.451316293003163E-5	0.0
4	5.542171564553215E-4	1.8171054310010542E-5	0.0	9.085527155005272E-5	0.0
5	0.0021623554628912548	2.7256581465015813E-5	0.0	2.6348028749515287E-4	0.0
6	0.005233263641283036	2.7256581465015813E-5	0.0	5.451316293003163E-4	0.0
7	0.006005533449458485	2.7256581465015813E-5	0.0	6.087303193853532E-4	0.0
8	0.00722299408822919	2.7256581465015813E-5	0.0	6.632434823153848E-4	0.0
9	0.008131546803729718	9.085527155005272E-5	0.0	7.177566452454164E-4	0.0
10-11	0.015713419214581616	9.085527155005272E-5	0.0	0.0012492599838132249	0.0
12-13	0.028305959851418924	9.994079870505799E-5	0.0	0.002153269935736249	2.7256581465015813E-5
14-15	0.043696842851997854	1.3628290732507908E-4	0.0	0.0032980463572669138	2.7256581465015813E-5
16-17	0.05074721192428194	1.3628290732507908E-4	0.0	0.003902233913074764	2.7256581465015813E-5
18-19	0.0542860247511565	1.4536843448008434E-4	0.0	0.004143000382682404	3.6342108620021084E-5
20-21	0.06717838778410898	1.5445396163508963E-4	0.0	0.005074266916070444	4.542763577502636E-5
22-23	0.07089891115408364	1.7262501594510015E-4	0.0	0.006200872283291098	4.542763577502636E-5
24-25	0.08767533704580087	1.7262501594510015E-4	9.085527155005271E-6	0.009421691659740467	4.542763577502636E-5
26-27	0.13302574584000967	1.7262501594510015E-4	9.085527155005271E-6	0.016317606770389466	4.542763577502636E-5
28-29	0.14288354280319038	1.7262501594510015E-4	9.085527155005271E-6	0.03180388780609595	4.542763577502636E-5
30-31	0.14654046748308003	2.1805265172012653E-4	9.085527155005271E-6	0.06630617717722848	5.451316293003163E-5
32-33	0.1499429974026295	2.3622370603013706E-4	9.085527155005271E-6	0.1221957974712434	6.35986900850369E-5
34-35	0.15246423118814345	2.7710857822766077E-4	9.085527155005271E-6	0.1964881530177215	6.35986900850369E-5
36-37	0.15956911342335758	3.270789775801898E-4	9.085527155005271E-6	0.30754509419692844	6.35986900850369E-5
38-39	0.16693293318248936	3.543355590452056E-4	9.085527155005271E-6	0.4777669882095297	7.268421724004217E-5
40-41	0.17258867383648013	3.543355590452056E-4	9.085527155005271E-6	0.7091799076110914	7.268421724004217E-5
42-43	0.18601254020800043	3.5887832262270825E-4	9.085527155005271E-6	0.8960328590809299	7.722698081754481E-5
44-45	0.20157150546094693	3.8613490408772406E-4	9.085527155005271E-6	1.0854161298634373	8.631250797255007E-5
46-47	0.20663214408628489	4.088487219752372E-4	9.085527155005271E-6	1.3088928412951017	9.994079870505798E-5
48-49	0.21048440760000714	4.179342491302425E-4	9.085527155005271E-6	1.5411643430128117	1.0902632586006325E-4
50-51	0.21762108918026377	4.997039935252899E-4	9.085527155005271E-6	1.803499854086434	1.0902632586006325E-4
52-53	0.21975618806169	4.997039935252899E-4	9.085527155005271E-6	2.105480062900922	1.1356908943756588E-4
54-55	0.22615239917881372	5.315033385678083E-4	9.085527155005271E-6	2.3686151003641847	1.1811185301506853E-4
56-57	0.2390674760296537	5.451316293003163E-4	9.085527155005271E-6	2.6772232012382484	1.2265461659257114E-4
58-59	0.24359661131642385	5.542171564553215E-4	9.085527155005271E-6	3.0120521334816575	1.271973801700738E-4
60-61	0.24687648661938072	5.814737379203374E-4	9.085527155005271E-6	3.3154860266409463	1.271973801700738E-4
62-63	0.24993376650704	5.814737379203374E-4	9.085527155005271E-6	3.6201918936019357	1.271973801700738E-4
64-65	0.2523505167302714	5.996447922303479E-4	9.085527155005271E-6	3.9617123165950057	1.271973801700738E-4
66-67	0.2555395367616783	5.996447922303479E-4	9.085527155005271E-6	4.353353050138663	1.271973801700738E-4
68-69	0.2586922146844651	6.541579551603795E-4	9.085527155005271E-6	4.782208102909222	1.271973801700738E-4
70-71	0.2627716163770625	6.768717730478927E-4	9.085527155005271E-6	5.250635169203406	1.3628290732507908E-4
72-73	0.2748917096018395	6.85957300202898E-4	9.085527155005271E-6	5.697883871699274	1.4991119805758698E-4
74-75	0.2875887338009594	7.35927699555427E-4	9.085527155005271E-6	6.132449178286754	1.7262501594510015E-4
76-77	0.291354684806709	7.677270445979455E-4	9.085527155005271E-6	6.563816379315671	1.8171054310010544E-4
78-79	0.29395314557304053	7.813553353304534E-4	9.085527155005271E-6	7.017779286379087	1.8171054310010544E-4
80-81	0.2992318368500986	7.904408624854586E-4	9.085527155005271E-6	7.530648208751979	1.8171054310010544E-4
82-83	0.30106257057183217	7.904408624854586E-4	9.085527155005271E-6	8.0663490608654	2.0896712456512125E-4
84-85	0.3076813771042535	7.904408624854586E-4	9.085527155005271E-6	8.599555935774772	2.0896712456512125E-4
86-87	0.31952890451438043	7.995263896404639E-4	9.085527155005271E-6	9.122250855765802	2.1350988814262386E-4
88	0.3224362732039821	8.449540254154902E-4	9.085527155005271E-6	9.524339945537717	2.180526517201265E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	2635	0.0	23.008867	1
GATCTAC	5795	0.0	20.681065	1
TCTACAC	8130	0.0	19.422152	3
CTACACT	9450	0.0	19.047384	4
TACACTC	7350	0.0	18.930134	5
GTATAAG	3780	0.0	18.65029	1
CATGGGG	22915	0.0	17.678923	4
TCTAACG	1575	0.0	17.306763	2
CTAACGC	1615	0.0	16.877346	3
TGATCGC	2390	0.0	16.129486	9
GTATAAT	3070	0.0	15.462124	1
ATCTACA	8255	0.0	15.3145275	2
GTCCTAC	3590	0.0	15.18622	1
GTGATCG	2555	0.0	15.086485	8
ATTTAGG	4370	0.0	14.626609	1
ATAAGGT	3665	0.0	14.617717	3
GTATATA	3225	0.0	14.427518	1
GGGCTAT	4650	0.0	14.152472	6
TTTAGGG	5115	0.0	14.057696	2
CTAGGCA	5510	0.0	13.988162	4
>>END_MODULE