FastQCFastQC Report
Fri 27 May 2016
ERR765358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6607931
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA361580.5471909437311013No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT317350.48025622543576807No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG146290.2213854835953947No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC100780.15251369906858894No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG100660.15233209910938839No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC94870.14356990107796225No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT93300.1411939682784218No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG89310.13515576963500375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20150.021.0026251
GTACAAG30850.020.501751
GTATAAG22900.019.6989691
GTATATA19050.018.3093761
TAGTACT28200.017.313114
CATGGGG139850.017.2559874
CTAGTAC26950.016.9112053
ATGGGTA39050.016.6702445
TACATGA25450.016.4460642
GTATAAT17750.016.2442841
GTACATA15850.016.1376631
GGGTACC40650.015.7836517
CCTAGTA27200.015.7299062
CATGGGT55750.015.5966624
TAGGCAT29550.015.2633275
ATACGGG13400.014.9235073
GTACAAA35650.014.8714241
ATAGGAC44750.014.8610743
GTATTAC10700.014.7775481
GTGATCG14800.014.7646048
Produced by FastQC (version 0.11.5)