FastQCFastQC Report
Fri 27 May 2016
ERR765358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6607931
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT351600.5320878804575895No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA344860.5218880160824924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157520.2383802131105788No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG143940.21782915106105072No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC109290.1653921628418941No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT104670.1584005644126732No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC103670.15688723141933533No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG96450.14596096720743604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96250.14565830060876847No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG95810.1449924340916998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87020.13169023708026006No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC68750.10404164329197746No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6000.034.1049352
ATCACGC10050.022.6728613
AGTACTT235150.022.21780612-13
GTACTAG20800.022.146981
TCACGCA11400.019.9879194
CTAACGC7300.019.7476393
TAGGCAT30900.019.5641615
TACTTTT272800.019.3984814-15
CTAGGCA32100.019.2673934
GTATAAG22900.018.4904961
GACGTTA7400.018.2234027
GAGTACT225650.017.7847812-13
CGTTAAC8300.016.8080069
CATGGGG115950.016.7641644
TCTAACG8050.016.7539772
ATACGGG14850.015.9705573
GTATCAA631850.015.8773281
CTAGTAC28500.015.8271683
GTACTTT258150.015.68974214-15
TACGGGC15500.015.6008414
Produced by FastQC (version 0.11.5)