FastQCFastQC Report
Fri 27 May 2016
ERR765357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10445534
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA691130.6616511898769369No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT567260.5430646245562937No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG313180.29982191432242716No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG253940.24310868166242147No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT253770.24294593268280973No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG246350.2358424183962256No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA207250.1984101530855196No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC201330.19274265920727462No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC189840.18174274287939707No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC166460.1593599714480849No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC156670.14998754491632502No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT151930.14544972042597343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125470.12011832042287164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC71700.021.0395093
TACACTC60100.020.883495
CATGGGG202400.020.6658954
GTACTAG31250.019.7017291
CTACACT85400.019.5914634
GTATTAG27850.018.3943461
TCTAACG15600.017.1717512
TAGTACT40450.016.7308774
GTATAAG40200.016.4845121
CTAGTAC41400.016.0054743
CTAACGC16250.015.905933
GTATATA30250.015.5366591
ATCTACA76600.015.3995932
ATGGGGG127800.015.3013125
TATTAAG31050.014.8329932
CTAGGCA52800.014.6867184
TAGGACT44850.014.5655574
ACACTCT91100.014.5514876
GTATAAA77150.014.3766961
TAGGCAT50600.014.3066015
Produced by FastQC (version 0.11.5)