##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765357_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10445534
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.883867785026595	33.0	33.0	33.0	27.0	33.0
2	31.840697086429472	33.0	33.0	33.0	27.0	33.0
3	31.887290491802524	33.0	33.0	33.0	27.0	33.0
4	35.63983583797631	37.0	37.0	37.0	33.0	37.0
5	35.62228173303538	37.0	37.0	37.0	33.0	37.0
6	35.62768155270951	37.0	37.0	37.0	33.0	37.0
7	35.50986794930733	37.0	37.0	37.0	33.0	37.0
8	35.45748460538255	37.0	37.0	37.0	33.0	37.0
9	35.468072671057314	37.0	37.0	37.0	33.0	37.0
10-11	35.42754812726664	37.0	37.0	37.0	33.0	37.0
12-13	35.42981124756284	37.0	37.0	37.0	33.0	37.0
14-15	37.05411508880255	40.0	37.0	40.0	33.0	40.0
16-17	37.06774072057972	40.0	37.0	40.0	33.0	40.0
18-19	37.086292524633016	40.0	37.0	40.0	33.0	40.0
20-21	37.09052610426619	40.0	37.0	40.0	33.0	40.0
22-23	37.04047394800496	40.0	37.0	40.0	33.0	40.0
24-25	36.99028738023351	40.0	37.0	40.0	33.0	40.0
26-27	36.922154721816995	40.0	37.0	40.0	33.0	40.0
28-29	36.8566029271457	40.0	37.0	40.0	33.0	40.0
30-31	36.800952158118484	40.0	37.0	40.0	33.0	40.0
32-33	36.70759642350501	38.5	37.0	40.0	33.0	40.0
34-35	36.62309251015793	37.0	37.0	40.0	33.0	40.0
36-37	36.5246836111969	37.0	37.0	40.0	33.0	40.0
38-39	36.42427361779685	37.0	37.0	40.0	33.0	40.0
40-41	36.31014388541553	37.0	37.0	40.0	33.0	40.0
42-43	36.18555437184925	37.0	37.0	40.0	33.0	40.0
44-45	35.991006395651965	37.0	37.0	40.0	33.0	40.0
46-47	35.777849748993205	37.0	37.0	40.0	33.0	40.0
48-49	35.537082833678014	37.0	37.0	40.0	27.0	40.0
50-51	35.3248914320704	37.0	37.0	40.0	27.0	40.0
52-53	35.12437042471932	37.0	35.0	40.0	27.0	40.0
54-55	34.9489556493713	37.0	33.0	40.0	27.0	40.0
56-57	34.75825496331734	37.0	33.0	40.0	27.0	40.0
58-59	34.51825047910428	37.0	33.0	40.0	27.0	40.0
60-61	34.27825116456468	37.0	33.0	38.5	27.0	40.0
62-63	33.99063733840701	37.0	33.0	37.0	27.0	40.0
64-65	33.72965039413016	37.0	33.0	37.0	27.0	40.0
66-67	33.504678602357714	37.0	33.0	37.0	27.0	40.0
68-69	33.22842628246674	37.0	33.0	37.0	27.0	40.0
70-71	32.92305309618446	37.0	33.0	37.0	27.0	38.5
72-73	32.62862056645453	37.0	33.0	37.0	27.0	37.0
74-75	32.37963047174036	37.0	33.0	37.0	22.0	37.0
76-77	32.092418970633766	35.0	33.0	37.0	22.0	37.0
78-79	31.860726794819684	33.0	33.0	37.0	22.0	37.0
80-81	31.666323330142816	33.0	33.0	37.0	22.0	37.0
82-83	31.489073129243558	33.0	33.0	37.0	22.0	37.0
84-85	31.304368354935228	33.0	33.0	37.0	22.0	37.0
86-87	31.10661412810489	33.0	33.0	37.0	22.0	37.0
88-89	30.898113681885484	33.0	33.0	37.0	18.5	37.0
90-91	30.71204205548515	33.0	33.0	37.0	15.0	37.0
92-93	30.508847561072514	33.0	33.0	37.0	15.0	37.0
94-95	30.26847837554308	33.0	33.0	37.0	15.0	37.0
96-97	30.037847131606675	33.0	33.0	37.0	4.0	37.0
98-99	29.711587315689172	33.0	30.0	37.0	2.0	37.0
100	29.417151004438836	33.0	27.0	37.0	2.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84493.0
3	19004.0
4	11695.0
5	10001.0
6	10738.0
7	14085.0
8	15783.0
9	15575.0
10	16699.0
11	19102.0
12	22469.0
13	26026.0
14	28161.0
15	31829.0
16	36325.0
17	42054.0
18	40973.0
19	34186.0
20	30999.0
21	32118.0
22	37447.0
23	46400.0
24	58235.0
25	70057.0
26	83056.0
27	100204.0
28	125330.0
29	161965.0
30	212132.0
31	284569.0
32	392583.0
33	553223.0
34	801460.0
35	1227490.0
36	2024449.0
37	2790776.0
38	933385.0
39	458.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.68345185709135	18.4548662206045	12.680449621163742	25.181232301140405
2	15.449805400305488	21.112819758142773	37.33845793397925	26.098916907572494
3	17.941743081187834	26.158884821128602	29.564748159765863	26.334623937917705
4	13.03364294801074	17.621398630030217	36.42973863562592	32.915219786333125
5	13.84574626777072	37.608196446947595	33.887006545499666	14.659050739782014
6	31.750857861932914	36.39791237161271	17.723233185456145	14.12799658099823
7	27.1913987487815	32.04083262152162	22.275168503590844	18.492600126106037
8	26.997781747977996	33.28687956498283	20.438148342707393	19.27719034433178
9	27.311226004117	14.897046292306957	19.19304897314376	38.59867873043228
10-11	24.717533010155545	25.85721991366127	27.67827738857066	21.74696968761252
12-13	26.207711539543272	23.47788829673282	27.094616379810738	23.21978378391317
14-15	24.22683625193377	23.95728115666844	24.75102009475119	27.064862496646597
16-17	23.716034849480746	26.77444588435177	25.89700455126674	23.612514714900744
18-19	24.292928497468658	26.368985877503604	26.921475212781015	22.41661041224672
20-21	24.68606393878984	25.82418986975531	26.34104983512373	23.14869635633112
22-23	24.275497951248543	25.89853438492171	25.422919400457523	24.403048263372224
24-25	24.10981521290787	26.256822423392194	25.290983466861526	24.342378896838408
26-27	24.41118732003511	25.75883334518238	25.366953030615306	24.4630263041672
28-29	24.220857495471858	25.493658207528735	26.28871945700015	23.99676483999926
30-31	24.921032693431847	25.415907884208472	25.97353759939005	23.68952182296963
32-33	23.66866630114723	25.995554602026594	25.997656003704595	24.33812309312158
34-35	23.7355391443539	26.058528889161202	26.240737585013175	23.965194381471726
36-37	24.579174606523825	25.791836056535406	25.671793410234088	23.957195926706685
38-39	24.793040738750168	25.386729869435108	25.656438435794666	24.163790956020055
40-41	24.19518696393327	25.680265832181014	25.859969757506207	24.264577446379505
42-43	24.899224874477458	25.46242729189336	25.50812146128671	24.130226372342474
44-45	24.841022967327472	25.40854301943778	25.71955631947586	24.030877693758885
46-47	24.401493673199464	25.409255418433034	26.355292163643867	23.83395874472364
48-49	24.847873008165138	25.60342892966246	26.059795544080306	23.488902518092097
50-51	24.080273418621783	25.97918778783008	25.6569851729732	24.28355362057493
52-53	23.91234843457803	26.22083954532528	24.977660767309075	24.889151252787617
54-55	24.156163005616236	25.890893108626443	24.9539217777382	24.99902210801912
56-57	24.58462386077928	25.221856278493537	25.134731759671553	25.05878810105563
58-59	24.322074958844052	25.181999357747912	26.05267424822938	24.443251435178652
60-61	24.551776465555474	25.501272867978457	25.592441683799922	24.354508982666147
62-63	23.554894413426144	25.658855289430953	25.830794801309846	24.955455495833057
64-65	23.413744198108127	25.65496798309872	25.85258432100408	25.078703497789075
66-67	24.417922278624076	25.453810222896404	25.410231686804863	24.718035811674653
68-69	24.392097409957895	24.999272282564252	25.564057614538417	25.044572692939436
70-71	23.846032128665954	25.373181490552355	25.604402365016828	25.176384015764864
72-73	24.558765879286305	25.15327977055577	25.50903821600062	24.77891613415731
74-75	24.55817457997004	25.010854640977215	25.684266082403155	24.746704696649587
76-77	24.058702483811505	25.251225940462152	26.02403697819888	24.66603459752746
78-79	24.18609420874974	25.283974512359336	25.939520904589674	24.59041037430125
80-81	23.802623626521584	25.374554341334743	25.886588770897635	24.936233261246038
82-83	23.792839654279536	25.476735835112187	25.333525856671002	25.39689865393727
84-85	23.95917336091293	25.472576783717642	25.171513452738203	25.39673640263122
86-87	23.943858122513486	24.963020522323482	25.555941690172656	25.537179664990372
88-89	24.00177731642457	24.554742016200777	25.91577760945195	25.5277030579227
90-91	24.206636536142025	24.883428961598185	25.721708102921326	25.188226399338465
92-93	23.600824202855218	25.03395755024925	25.875550348361447	25.48966789853409
94-95	23.09041148042045	25.1531421391727	26.114326597329363	25.642119783077483
96-97	23.838379350366413	24.913134990984215	25.6403013232266	25.608184335422774
98-99	23.86787086746972	24.77485627038016	25.528114522370792	25.829158339779323
100	23.63981694818664	24.849898355612883	25.80807096264205	25.702213733558427
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2355.0
1	2083.5
2	1806.5
3	1694.0
4	1321.5
5	1203.5
6	1640.5
7	3119.5
8	5035.5
9	4694.0
10	2768.5
11	1823.0
12	1784.0
13	1911.5
14	2109.0
15	2466.5
16	2823.5
17	3265.0
18	3963.5
19	4597.0
20	5420.0
21	6678.5
22	8703.5
23	11224.5
24	13899.5
25	17764.5
26	23809.5
27	30956.0
28	38224.5
29	49266.0
30	62531.0
31	75172.0
32	89755.5
33	104826.5
34	120914.5
35	135549.5
36	151167.0
37	172147.5
38	190465.0
39	207105.5
40	220005.0
41	231439.0
42	246035.0
43	268899.0
44	295444.0
45	323487.0
46	359532.5
47	396020.0
48	459763.5
49	521652.0
50	595851.5
51	595918.0
52	505068.5
53	464517.0
54	444238.5
55	412587.0
56	379990.5
57	343677.0
58	313938.0
59	286499.0
60	246821.0
61	206117.5
62	166664.0
63	132290.0
64	101115.0
65	75926.5
66	58667.5
67	45361.5
68	35585.5
69	28871.0
70	23705.5
71	19275.0
72	15429.0
73	13633.0
74	11330.0
75	8789.0
76	6686.0
77	4873.0
78	3752.5
79	2658.0
80	1890.0
81	1393.5
82	995.5
83	721.5
84	533.0
85	338.5
86	219.5
87	146.5
88	95.5
89	65.5
90	45.5
91	30.0
92	16.5
93	8.5
94	7.0
95	8.5
96	5.5
97	3.0
98	4.5
99	8.5
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006241902041580641
2	0.012187026532104534
3	0.010291479593096916
4	0.017232244900069252
5	0.010588257144153665
6	0.0036379183677923985
7	0.0034081551024581413
8	0.020831869390305943
9	0.009898967348150893
10-11	0.013761862246583085
12-13	0.010736645919682038
14-15	0.008769297960257464
16-17	0.011382855103434635
18-19	0.011196172450350553
20-21	0.00700777959269483
22-23	0.006088726531357803
24-25	0.012426363266827717
26-27	0.015858452042758178
28-29	0.0028816142860671363
30-31	6.892897960027703E-4
32-33	0.0011966836736159205
34-35	8.999061225591721E-4
36-37	7.610908164197254E-4
38-39	0.0
40-41	0.0028481071432058906
42-43	0.0
44-45	0.0
46-47	9.094795919480996E-5
48-49	0.006146167347691367
50-51	0.01272792755257893
52-53	0.019376702043188983
54-55	0.020597319390277223
56-57	0.034296954085832276
58-59	0.039739471433437486
60-61	0.032233871432518435
62-63	0.022272676533339513
64-65	0.01977400102282947
66-67	0.02220566224761702
68-69	0.01838106122674054
70-71	0.019687839798329124
72-73	0.02117651428830733
74-75	0.01765826428787652
76-77	0.009587829593010754
78-79	0.016533381634677557
80-81	0.009779298980789303
82-83	0.017026415308207317
84-85	0.024397986737681385
86-87	0.014987266328365787
88-89	0.027035477554330875
90-91	0.030529793881289364
92-93	0.02600632959502118
94-95	0.017280112247013892
96-97	0.023574668370233635
98-99	0.016327552042815618
100	0.01223489387904917
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.0445534E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.181959283800325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.24483802726182	28.306943020489218
2	13.91323507034954	10.902956200647568
3	5.221120511214124	6.137211938586196
4	2.4654955324082866	3.8641178226085233
5	1.4028692334500348	2.748358259276772
6	0.9024930881632164	2.1216868460593417
7	0.6239065045300761	1.7112115480176937
8	0.48206977811394836	1.5110750734409106
9	0.3283583143401455	1.15791498926756
>10	2.0913032881024547	15.393627737854668
>50	0.18242349330429464	4.963389619919274
>100	0.1231161946719211	9.549970062943293
>500	0.012296565601735605	3.296297579512608
>1k	0.005717016408837326	3.8176000878610012
>5k	4.3977023977112063E-4	1.1436254275726918
>10k+	3.176118398346983E-4	3.3740137859425765
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	69113	0.6616511898769369	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	56726	0.5430646245562937	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	31318	0.29982191432242716	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	25394	0.24310868166242147	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	25377	0.24294593268280973	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	24635	0.2358424183962256	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	20725	0.1984101530855196	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	20133	0.19274265920727462	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	18984	0.18174274287939707	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	16646	0.1593599714480849	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	15667	0.14998754491632502	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	15193	0.14544972042597343	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12547	0.12011832042287164	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7232244900069254E-4	0.0	0.0	0.0	2.872040816678209E-5
2	2.3933673472318408E-4	9.573469388927364E-6	0.0	1.9146938777854728E-5	2.872040816678209E-5
3	2.4891020411211146E-4	9.573469388927364E-6	0.0	1.9146938777854728E-5	2.872040816678209E-5
4	2.680571428899662E-4	1.9146938777854728E-5	0.0	2.872040816678209E-5	2.872040816678209E-5
5	0.0012732714287273393	2.872040816678209E-5	0.0	1.5317551022283783E-4	2.872040816678209E-5
6	0.003293273469791013	2.872040816678209E-5	0.0	3.446448980013851E-4	4.786734694463682E-5
7	0.003867681633126655	3.8293877555709456E-5	0.0	3.7336530616816717E-4	4.786734694463682E-5
8	0.004738867347519045	3.8293877555709456E-5	0.0	4.5952653066851345E-4	4.786734694463682E-5
9	0.005217540816965413	4.786734694463682E-5	0.0	5.073938776131503E-4	4.786734694463682E-5
10-11	0.01036328061351387	4.786734694463682E-5	0.0	8.472520409200717E-4	4.786734694463682E-5
12-13	0.01852466326757445	4.786734694463682E-5	0.0	0.0016370632655065792	1.05308163278201E-4
14-15	0.027877942860556483	5.744081633356418E-5	0.0	0.002465168367648796	1.9146938777854728E-4
16-17	0.03193709388146168	5.744081633356418E-5	0.0	0.0027763061227889354	2.3933673472318408E-4
18-19	0.03389486837149733	5.744081633356418E-5	0.0	0.0029390551024007006	2.7763061227889356E-4
20-21	0.041362174494860675	5.744081633356418E-5	0.0	0.0034703826534861694	3.0156428575121196E-4
22-23	0.043520991842063796	5.744081633356418E-5	0.0	0.004197966327044649	3.4943163269584877E-4
24-25	0.05433422551685725	5.744081633356418E-5	0.0	0.0058446030619401556	3.6379183677923984E-4
26-27	0.08352852041839125	5.744081633356418E-5	0.0	0.00920489081745366	3.8293877555709455E-4
28-29	0.09002411939877847	5.744081633356418E-5	0.0	0.017222671430680327	3.8293877555709455E-4
30-31	0.09261852960317778	5.744081633356418E-5	0.0	0.03513463265736343	3.9251224494602193E-4
32-33	0.09479649388915876	5.744081633356418E-5	0.0	0.06345774184450503	4.1165918372387664E-4
34-35	0.09657237246080477	7.658775511141891E-5	0.0	0.10066503062457123	4.21232653112804E-4
36-37	0.10184735409410375	1.0052142858373732E-4	0.0	0.16001575410122643	4.21232653112804E-4
38-39	0.10710797552331935	1.1488163266712836E-4	0.0	0.2598191724807942	4.3559285719619505E-4
40-41	0.11120542042178025	1.2445510205605573E-4	0.0	0.40746121739683194	4.4037959189065874E-4
42-43	0.12111396123932008	1.2445510205605573E-4	0.0	0.5272109592482299	4.4516632658512243E-4
44-45	0.13267871226114433	1.292418367505194E-4	0.0	0.6572234602845579	4.691000000574408E-4
46-47	0.1365033132820208	1.340285714449831E-4	0.0	0.8131657031607958	4.930336735297592E-4
48-49	0.1390881500170312	1.340285714449831E-4	0.0	0.960089737872664	5.839816327245692E-4
50-51	0.14361640103799383	1.4360204083391044E-4	0.0	1.1320627552406608	5.935551021134965E-4
52-53	0.1450093408340828	1.4360204083391044E-4	0.0	1.327217928733945	6.509959184470607E-4
54-55	0.1493556959366558	1.5317551022283783E-4	0.0	1.4943802777340056	7.084367347806249E-4
56-57	0.15880949695822155	1.7232244900069254E-4	0.0	1.7047859879638514	7.180102041695523E-4
58-59	0.16170068471367763	1.7232244900069254E-4	0.0	1.9345301063593303	7.419438776418708E-4
60-61	0.16407011838743715	1.8668265308408358E-4	0.0	2.1479227390385214	7.658775511141891E-4
62-63	0.16647784593875237	1.9146938777854728E-4	0.0	2.381692501312044	8.137448980588259E-4
64-65	0.16799045410220292	1.9146938777854728E-4	0.0	2.619291651341138	8.137448980588259E-4
66-67	0.17014927144940603	1.9146938777854728E-4	0.0	2.880173478924103	8.185316327532896E-4
68-69	0.17261443981705482	1.9146938777854728E-4	0.0	3.197720671820129	8.233183674477533E-4
70-71	0.17586941940929013	1.9146938777854728E-4	0.0	3.5232329912477427	8.28105102142217E-4
72-73	0.18537587451249501	1.9146938777854728E-4	0.0	3.821580591284275	8.951193878647084E-4
74-75	0.19529877553411823	1.9146938777854728E-4	0.0	4.145297885201465	9.094795919480995E-4
76-77	0.19837664594265836	1.9625612247301097E-4	0.0	4.493456246468586	9.190530613370269E-4
78-79	0.2003104867592217	2.0104285716747463E-4	0.0	4.842007119980654	9.33413265420418E-4
80-81	0.20404413982090336	2.0104285716747463E-4	0.0	5.215415506761071	9.90854081753982E-4
82-83	0.2052934775761584	2.10616326556402E-4	0.0	5.646676369058777	0.0010626551021709373
84-85	0.2106450469645688	2.2018979594532937E-4	0.0	6.100769955849074	0.0010626551021709373
86-87	0.220237663292274	2.2018979594532937E-4	0.0	6.512802504879119	0.0010913755103377195
88	0.2224012673741716	2.2976326533425672E-4	0.0	6.832202164101902	0.0011200959185045016
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACAC	7170	0.0	21.039509	3
TACACTC	6010	0.0	20.88349	5
CATGGGG	20240	0.0	20.665895	4
GTACTAG	3125	0.0	19.701729	1
CTACACT	8540	0.0	19.591463	4
GTATTAG	2785	0.0	18.394346	1
TCTAACG	1560	0.0	17.171751	2
TAGTACT	4045	0.0	16.730877	4
GTATAAG	4020	0.0	16.484512	1
CTAGTAC	4140	0.0	16.005474	3
CTAACGC	1625	0.0	15.90593	3
GTATATA	3025	0.0	15.536659	1
ATCTACA	7660	0.0	15.399593	2
ATGGGGG	12780	0.0	15.301312	5
TATTAAG	3105	0.0	14.832993	2
CTAGGCA	5280	0.0	14.686718	4
TAGGACT	4485	0.0	14.565557	4
ACACTCT	9110	0.0	14.551487	6
GTATAAA	7715	0.0	14.376696	1
TAGGCAT	5060	0.0	14.306601	5
>>END_MODULE