FastQCFastQC Report
Fri 27 May 2016
ERR765355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765355_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12214068
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT434170.35546715475957724No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA411970.33729139218809No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252180.2064668380755699No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA198130.1622145873103048No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA179730.14714999130510817No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT157870.12925259626849955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153990.1260759314587081No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG151660.12416829511674571No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG139740.11440905683511833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134620.11021716925106362No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA132680.10862883684616788No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT128480.10519017906237299No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG127620.1044860729447388No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA123650.10123572261100887No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG123620.10121116076969607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA21400.027.017641
CTATTGA59600.025.4602899
AGGGCTA64100.024.7782545
GGGCTAT71300.023.0005916
TTTAGGG69950.022.7776722
TAGTACT55700.022.6114774
ACGTTAA13000.022.406648
ATTTAGG71400.022.3839511
TACATGA36800.022.0950852
GTACTAG48250.022.0174581
GGCTATT75650.021.4280687
CGCAGAT29450.021.2175278
CTGTGCG65000.020.6709189
CATGGGT83500.020.317494
CATGGGG404900.020.1488974
GTACAAA44750.019.7478981
TAGGCAT68900.019.6419815
GTATAAG45050.019.3033641
CTAGTAC71700.018.9426383
GTATCAA977100.018.704381
Produced by FastQC (version 0.11.5)