##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765355_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12214068
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65227277267492	33.0	33.0	33.0	27.0	33.0
2	31.559089731611124	33.0	33.0	33.0	27.0	33.0
3	31.5119083993965	33.0	33.0	33.0	27.0	33.0
4	35.07094941668902	37.0	37.0	37.0	33.0	37.0
5	35.3476378222227	37.0	37.0	37.0	33.0	37.0
6	35.34023733943515	37.0	37.0	37.0	33.0	37.0
7	35.19404214877468	37.0	37.0	37.0	33.0	37.0
8	35.15175001481898	37.0	37.0	37.0	33.0	37.0
9	34.802945423261114	37.0	37.0	37.0	33.0	37.0
10-11	34.83449322535293	37.0	37.0	37.0	33.0	37.0
12-13	34.8037591161274	37.0	37.0	37.0	33.0	37.0
14-15	36.07737327972957	37.0	37.0	40.0	30.0	40.0
16-17	36.35461997591629	37.0	37.0	40.0	33.0	40.0
18-19	36.18882926638365	37.0	37.0	40.0	27.0	40.0
20-21	36.201972389542945	37.0	37.0	40.0	27.0	40.0
22-23	36.40031949224452	37.0	37.0	40.0	33.0	40.0
24-25	36.41955120931044	37.0	37.0	40.0	33.0	40.0
26-27	36.09086669568239	37.0	37.0	40.0	27.0	40.0
28-29	35.96083282817813	37.0	37.0	40.0	27.0	40.0
30-31	35.752997486177414	37.0	37.0	40.0	27.0	40.0
32-33	35.6984486659154	37.0	35.0	40.0	27.0	40.0
34-35	35.62516828954939	37.0	35.0	40.0	27.0	40.0
36-37	35.35478126534092	37.0	33.0	40.0	27.0	40.0
38-39	35.178548252719736	37.0	33.0	40.0	27.0	40.0
40-41	35.27906173438694	37.0	33.0	40.0	27.0	40.0
42-43	35.156277376218966	37.0	33.0	40.0	27.0	40.0
44-45	35.062066135541414	37.0	33.0	40.0	27.0	40.0
46-47	34.95792511553071	37.0	33.0	40.0	27.0	40.0
48-49	34.746337542905444	37.0	33.0	40.0	27.0	40.0
50-51	34.56304205936957	37.0	33.0	40.0	27.0	40.0
52-53	34.437757019201136	37.0	33.0	40.0	27.0	40.0
54-55	34.28200678103315	37.0	33.0	40.0	27.0	40.0
56-57	34.060285565791844	37.0	33.0	37.0	27.0	40.0
58-59	33.87043100627899	37.0	33.0	37.0	27.0	40.0
60-61	33.58725872493915	37.0	33.0	37.0	22.0	40.0
62-63	33.36020210465506	37.0	33.0	37.0	22.0	40.0
64-65	33.17726379122828	37.0	33.0	37.0	22.0	40.0
66-67	32.965237585053565	37.0	33.0	37.0	22.0	40.0
68-69	32.78367399788506	37.0	33.0	37.0	22.0	37.0
70-71	32.587127605642934	37.0	33.0	37.0	22.0	37.0
72-73	32.379854033889444	33.0	33.0	37.0	22.0	37.0
74-75	32.17074053460321	33.0	33.0	37.0	22.0	37.0
76-77	31.988206795639258	33.0	33.0	37.0	22.0	37.0
78-79	31.82432957635409	33.0	33.0	37.0	22.0	37.0
80-81	31.70436487663242	33.0	33.0	37.0	22.0	37.0
82-83	31.591050704810222	33.0	33.0	37.0	22.0	37.0
84-85	31.431147468640262	33.0	33.0	37.0	22.0	37.0
86-87	31.228296297351545	33.0	30.0	37.0	18.5	37.0
88-89	31.08766460936684	33.0	27.0	37.0	18.5	37.0
90-91	31.01043321520725	33.0	27.0	37.0	15.0	37.0
92-93	30.914462609836463	33.0	27.0	37.0	15.0	37.0
94-95	30.78864277650984	33.0	27.0	37.0	15.0	37.0
96-97	30.705538318601143	33.0	27.0	37.0	15.0	37.0
98-99	30.574633201649114	33.0	27.0	37.0	15.0	37.0
100	30.48092764834779	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	184.0
8	1081.0
9	2193.0
10	3818.0
11	7106.0
12	13155.0
13	22822.0
14	33785.0
15	40978.0
16	47539.0
17	57623.0
18	70489.0
19	84525.0
20	100403.0
21	118452.0
22	135758.0
23	140330.0
24	138226.0
25	143907.0
26	162242.0
27	192098.0
28	233914.0
29	290073.0
30	360321.0
31	446762.0
32	576561.0
33	758565.0
34	1000367.0
35	1377126.0
36	2095991.0
37	2683044.0
38	873794.0
39	830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48507646626174	19.438745281449364	12.922859258811464	24.153318993477438
2	16.180934028991963	21.330451225414524	37.167661537126044	25.32095320846747
3	17.7233087787722	27.564522362017048	29.303541495837997	25.408627363372755
4	12.229495236299073	17.681678924403737	36.83027358738482	33.25855225191236
5	13.971552083872417	37.87201713190031	32.31706298488905	15.839367799338227
6	30.710876293873145	37.237799132988805	17.466995716537213	14.584328856600838
7	26.778033575537997	32.648301379546766	22.249878051227903	18.32378699368733
8	27.760268586589596	31.427599885632574	19.969585535992497	20.84254599178533
9	27.651533015392054	13.979754987400906	20.252569763351865	38.11614223385518
10-11	25.878221209487457	25.20059799602802	25.63797234659322	23.28320844789131
12-13	26.4815890774322	23.62391977111005	26.00708479421957	23.887406357238177
14-15	24.8154994994182	24.227131664639995	24.136328549341123	26.821040286600685
16-17	23.786782974911024	26.206167329318962	25.2135398700416	24.79350982572841
18-19	24.278309918844382	25.144060453357607	27.783572846621784	22.794056781176224
20-21	26.183468538201005	24.06696318380822	26.331351826099862	23.41821645189091
22-23	25.17902651355858	24.143094775847494	26.213893707881187	24.463985002712743
24-25	24.073343703264268	25.237123315425357	26.361716774441557	24.32781620686882
26-27	24.63915683112279	24.77503464714374	26.498184671886584	24.087623849846885
28-29	23.667529979185566	24.623942205309366	27.49060692526016	24.21792089024491
30-31	24.388994813454907	24.74342074116763	26.87713502070375	23.99044942467371
32-33	23.974336812564893	24.930106789069832	25.925261124607125	25.170295273758153
34-35	24.659400215748317	24.772610948217498	27.07354270260746	23.494446133426724
36-37	24.48190307276855	24.84550889149371	26.363121486043738	24.309466549694005
38-39	24.803538876533427	24.084334415165518	26.37057494005469	24.741551768246364
40-41	24.25808863104926	25.05635696626741	26.700036112529048	23.98551829015428
42-43	25.331309367552336	24.52965425943148	26.196034548047308	23.943001824968878
44-45	24.479858848467803	25.17715416772787	25.70526818864845	24.637718795155873
46-47	24.059028725024433	24.90618878738501	26.379961217161775	24.65482127042878
48-49	24.992200606943523	25.437974425528004	25.391485291180345	24.178339676348127
50-51	24.05120263916607	25.883065958471875	24.936581266826742	25.129150135535316
52-53	24.102599811847345	25.384016734541543	24.754788933715798	25.75859451989532
54-55	24.614010663769022	25.38833908571657	25.04402300691301	24.953627243601396
56-57	25.03885683295688	24.67563222998267	24.520470984769364	25.76503995229108
58-59	24.40343381091378	24.778538157802956	25.494773731405456	25.323254299877814
60-61	24.5622343022816	25.08415296197794	25.26172279374898	25.09188994199148
62-63	23.826025750935216	25.14740253461539	24.779918590162456	26.246653124286933
64-65	24.0722335993851	24.90854990720105	24.98835561275021	26.030860880663635
66-67	25.067875955711017	24.79142699139271	24.893576093384905	25.247120959511367
68-69	25.023108943378052	24.304566130713845	24.817719765846842	25.85460516006126
70-71	23.95381322647069	24.58379623908005	25.36047085737547	26.101919677073788
72-73	24.930720060658096	24.710555280530382	25.008438039735193	25.35028661907633
74-75	25.389804864230243	24.510637740440117	25.037203026675815	25.06235436865383
76-77	24.57260400898031	24.817575917773734	25.361527194882516	25.248292878363436
78-79	24.504452570593187	25.13798023721499	25.12511801964751	25.232449172544314
80-81	23.926803545241686	24.676930765413292	26.070877406340227	25.325388283004795
82-83	24.43363092547981	24.882692300134796	25.008285552223867	25.675391222161526
84-85	24.44706468667398	25.20497677908914	24.433776529799058	25.91418200443782
86-87	24.262446069695706	24.498134840160706	25.02889308031247	26.21052600983112
88-89	24.220833431071622	24.0562523869123	25.296887984224654	26.42602619779143
90-91	24.62239533706707	24.237918212569667	25.165838689418408	25.973847760944857
92-93	24.294609368702574	24.590919141206015	25.005286128678435	26.109185361412973
94-95	23.323815468344826	24.820741235752628	25.687579401112608	26.16786389478993
96-97	24.21789082615619	24.551428355279057	24.89809860478765	26.332582213777105
98-99	23.71987519306883	24.64236850369357	25.162543395839283	26.475212907398316
100	23.68310413311999	24.829750649741523	24.588439722171074	26.898705494967416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6292.0
1	4528.0
2	3395.5
3	3866.5
4	3025.5
5	2478.5
6	3084.5
7	5416.5
8	8450.5
9	8261.5
10	5172.5
11	3128.5
12	2635.0
13	2582.0
14	2878.5
15	3539.5
16	4336.5
17	5197.5
18	6403.5
19	7649.5
20	9159.0
21	10992.0
22	13281.5
23	16373.5
24	19941.0
25	24434.5
26	30917.0
27	37780.0
28	45961.0
29	60028.0
30	77595.0
31	93200.5
32	111984.5
33	131468.5
34	150101.5
35	165088.5
36	176616.0
37	198523.5
38	206998.0
39	202866.5
40	198698.0
41	192997.5
42	199363.5
43	229581.5
44	276966.5
45	326261.5
46	392785.0
47	455459.0
48	513536.0
49	559164.0
50	618006.5
51	617345.5
52	558128.0
53	549734.5
54	531222.5
55	509615.0
56	505534.0
57	479132.0
58	455262.0
59	433259.0
60	378214.0
61	294405.0
62	221090.0
63	166114.0
64	121396.5
65	103482.5
66	87223.0
67	61006.0
68	43609.0
69	35065.0
70	30729.0
71	29324.5
72	27499.5
73	30965.5
74	25813.5
75	18161.5
76	13858.5
77	11034.5
78	8768.5
79	5718.5
80	4538.5
81	4095.0
82	3932.5
83	3976.5
84	3271.5
85	2265.0
86	1689.0
87	896.5
88	401.0
89	286.5
90	232.0
91	165.0
92	103.0
93	60.0
94	40.5
95	34.0
96	25.0
97	17.0
98	10.5
99	6.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050228965484718106
2	0.025364194795706063
3	0.02258870672735734
4	0.023489307575494094
5	0.0163418117534633
6	0.025814495219774445
7	0.04972135409758648
8	0.006549824350085491
9	0.004028141975302577
10-11	0.0022474084801230843
12-13	0.0013386203515487224
14-15	0.002292438522529922
16-17	0.00634514233914532
18-19	0.005067926590878649
20-21	0.008666236343206866
22-23	0.01849916014877271
24-25	0.025560689526208633
26-27	0.02837302035652659
28-29	0.04480489219480356
30-31	0.04232823986242749
32-33	0.04017089146711808
34-35	0.04798974428503264
36-37	0.04517331981449587
38-39	0.05478109340802753
40-41	0.05234537747783949
42-43	0.05739692950784293
44-45	0.05767939068294036
46-47	0.06253444798244123
48-49	0.06541637069647885
50-51	0.033981307456287295
52-53	4.052703816615398E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.321732284444462E-4
64-65	2.3743113269059907E-4
66-67	4.871431860376085E-4
68-69	0.0014900850396444493
70-71	6.959188371965834E-5
72-73	2.8246117509743684E-4
74-75	6.549824350085493E-5
76-77	1.1871556634529953E-4
78-79	0.0
80-81	5.3217322844444614E-5
82-83	2.947420957538471E-4
84-85	0.0015105532407384665
86-87	0.004122295700335056
88-89	0.002689521623753855
90-91	0.003827553604581209
92-93	0.0040813592981470215
94-95	0.0030252001216957364
96-97	0.002456184131282059
98-99	0.003958550091582919
100	0.0029883573597265056
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2214068E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.30020370798906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.78456585762282	18.41464342995403
2	12.704023989090121	6.428287896703198
3	5.189190342098479	3.938627182038629
4	2.649445165804754	2.681260096320285
5	1.5780522595758035	1.9962521814560132
6	0.977257921709025	1.4834894696690615
7	0.6835641804924002	1.210601910975963
8	0.46748577902044725	0.9461988351844219
9	0.34391462809876794	0.7830999134150516
>10	2.119290154547049	10.090514193036473
>50	0.2144815287118827	3.7740109532228185
>100	0.20400497146288946	11.163950542308823
>500	0.04151573432297058	7.427486376551366
>1k	0.038692745904699864	19.60945611027118
>5k	0.0034918704083847464	6.112235859705661
>10k+	0.001022871129728865	3.939885049187022
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	43417	0.35546715475957724	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	41197	0.33729139218809	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25218	0.2064668380755699	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	19813	0.1622145873103048	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA	17973	0.14714999130510817	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	15787	0.12925259626849955	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15399	0.1260759314587081	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	15166	0.12416829511674571	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	13974	0.11440905683511833	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13462	0.11021716925106362	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	13268	0.10862883684616788	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	12848	0.10519017906237299	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	12762	0.1044860729447388	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	12365	0.10123572261100887	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	12362	0.10121116076969607	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.187280437606864E-6	0.0	1.6374560875213728E-5	0.0
3	0.0	1.6374560875213728E-5	0.0	2.4561841312820592E-5	0.0
4	0.0	1.6374560875213728E-5	0.0	2.4561841312820592E-5	0.0
5	0.0	1.6374560875213728E-5	0.0	3.2749121750427456E-5	0.0
6	0.0	1.6374560875213728E-5	0.0	3.2749121750427456E-5	0.0
7	0.0	1.6374560875213728E-5	0.0	3.2749121750427456E-5	8.187280437606864E-6
8	0.0	2.4561841312820592E-5	0.0	3.2749121750427456E-5	8.187280437606864E-6
9	0.0	3.2749121750427456E-5	0.0	3.2749121750427456E-5	8.187280437606864E-6
10-11	0.0	3.2749121750427456E-5	0.0	4.0936402188034317E-5	8.187280437606864E-6
12-13	0.0	3.2749121750427456E-5	0.0	6.549824350085491E-5	8.187280437606864E-6
14-15	4.093640218803432E-6	5.731096306324805E-5	0.0	8.187280437606865E-5	4.9123682625641184E-5
16-17	2.0468201094017158E-5	5.731096306324805E-5	0.0	9.415372503247894E-5	4.9123682625641184E-5
18-19	3.2749121750427456E-5	5.731096306324805E-5	0.0	1.2280920656410296E-4	4.9123682625641184E-5
20-21	3.6842761969230886E-5	5.731096306324805E-5	0.0	3.070230164102574E-4	4.9123682625641184E-5
22-23	5.321732284444462E-5	6.959188371965836E-5	0.0	0.0012731221080478673	4.9123682625641184E-5
24-25	7.368552393846177E-5	7.777916415726522E-5	0.0	0.00387667728720685	4.9123682625641184E-5
26-27	1.146219261264961E-4	8.187280437606865E-5	0.0	0.006496607027241047	4.9123682625641184E-5
28-29	1.4737104787692354E-4	8.187280437606865E-5	0.0	0.017266974442912877	6.549824350085491E-5
30-31	2.0058837072136818E-4	9.824736525128237E-5	0.0	0.05239450116046514	7.368552393846179E-5
32-33	2.0877565115897507E-4	9.824736525128237E-5	0.0	0.11578861358885509	7.368552393846179E-5
34-35	2.2105657181538534E-4	1.1052828590769266E-4	0.0	0.19845967780759038	7.777916415726522E-5
36-37	2.2105657181538534E-4	1.2280920656410296E-4	0.0	0.32796198612943694	8.187280437606865E-5
38-39	2.251502120341888E-4	1.2280920656410296E-4	0.0	0.5217180713256222	9.006008481367551E-5
40-41	2.292438522529922E-4	1.2280920656410296E-4	0.0	0.7619738157671957	9.006008481367551E-5
42-43	2.3333749247179562E-4	1.3509012722051324E-4	0.0	0.9723787357332545	9.006008481367551E-5
44-45	2.4971205334700934E-4	1.4737104787692354E-4	0.0	1.2171497653361683	9.824736525128237E-5
46-47	2.538056935658128E-4	1.5555832831453043E-4	0.0	1.478389509539328	9.824736525128237E-5
48-49	2.5789933378461623E-4	1.5555832831453043E-4	0.0	1.7522908829392467	9.824736525128237E-5
50-51	2.6199297400341965E-4	1.637456087521373E-4	0.0	2.056255950106058	9.824736525128237E-5
52-53	2.6199297400341965E-4	1.637456087521373E-4	0.0	2.357011603341327	1.5555832831453043E-4
54-55	2.701802544410265E-4	1.6783924897094074E-4	0.0	2.6449050390091164	1.637456087521373E-4
56-57	2.824611750974368E-4	1.7193288918974415E-4	0.0	2.966268077105842	1.637456087521373E-4
58-59	2.9474209575384714E-4	1.7193288918974415E-4	0.0	3.326205486984353	1.6783924897094074E-4
60-61	2.9474209575384714E-4	1.7193288918974415E-4	0.0	3.6802644295086617	1.7193288918974415E-4
62-63	3.02929376191454E-4	1.7193288918974415E-4	0.0	4.03562514962255	1.8012016962735101E-4
64-65	3.02929376191454E-4	1.7193288918974415E-4	0.0	4.410099075918032	2.1286929137777848E-4
66-67	3.02929376191454E-4	1.7602652940854757E-4	0.0	4.819323914030935	2.2105657181538534E-4
68-69	3.02929376191454E-4	1.8421380984615446E-4	0.0	5.254965012475777	2.2105657181538534E-4
70-71	3.1111665662906087E-4	1.8830745006495787E-4	0.0	5.751523571016634	2.2105657181538534E-4
72-73	3.152102968478643E-4	1.9649473050256474E-4	0.0	6.224199832520991	2.2105657181538534E-4
74-75	3.2339757728547117E-4	2.0877565115897507E-4	0.0	6.685896950958518	2.2105657181538534E-4
76-77	3.31584857723078E-4	2.2105657181538534E-4	0.0	7.1853538067742875	2.2105657181538534E-4
78-79	3.356784979418814E-4	2.2105657181538534E-4	0.0	7.6906768490236015	2.2105657181538534E-4
80-81	3.356784979418814E-4	2.2105657181538534E-4	0.0	8.24665459534039	2.2105657181538534E-4
82-83	3.356784979418814E-4	2.292438522529922E-4	0.0	8.84391260962359	2.2105657181538534E-4
84-85	3.356784979418814E-4	2.292438522529922E-4	0.0	9.41627719773625	2.2105657181538534E-4
86-87	3.356784979418814E-4	2.292438522529922E-4	0.0	10.016683221347712	2.2105657181538534E-4
88	3.356784979418814E-4	2.292438522529922E-4	0.0	10.48073418291105	2.2105657181538534E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATA	2140	0.0	27.01764	1
CTATTGA	5960	0.0	25.460289	9
AGGGCTA	6410	0.0	24.778254	5
GGGCTAT	7130	0.0	23.000591	6
TTTAGGG	6995	0.0	22.777672	2
TAGTACT	5570	0.0	22.611477	4
ACGTTAA	1300	0.0	22.40664	8
ATTTAGG	7140	0.0	22.383951	1
TACATGA	3680	0.0	22.095085	2
GTACTAG	4825	0.0	22.017458	1
GGCTATT	7565	0.0	21.428068	7
CGCAGAT	2945	0.0	21.217527	8
CTGTGCG	6500	0.0	20.670918	9
CATGGGT	8350	0.0	20.31749	4
CATGGGG	40490	0.0	20.148897	4
GTACAAA	4475	0.0	19.747898	1
TAGGCAT	6890	0.0	19.641981	5
GTATAAG	4505	0.0	19.303364	1
CTAGTAC	7170	0.0	18.942638	3
GTATCAA	97710	0.0	18.70438	1
>>END_MODULE