FastQCFastQC Report
Fri 27 May 2016
ERR765354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765354_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15594778
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1508010.966996772894106No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1381800.886065835627798No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG611800.392310810708559No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC416970.26737796459814944No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC408750.26210696939706357No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG391950.2513341324897347No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG379060.24306854512452822No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT362250.23228929581427835No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC262360.1682358030361189No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG226380.14516397732625627No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT223640.1434069789258943No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC186560.11962978889471848No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA182530.11704559051754376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178040.1141664216060017No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC162920.10447086838940574No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG159100.10202133047357263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT49850.019.892484
CATGGGG382800.019.2138354
CGTTAAC9850.018.1318151
GTATAAG59150.018.1164881
GTATTAC34500.017.9824451
GTACAAG57500.017.3285391
GTACATA33300.017.2190631
GTACTAG42250.017.1312071
CATGGGT118000.016.9269454
CTATTGA53300.016.8413859
ATTTAGG57300.016.7328321
TTTAGGG60800.016.6191162
TATTCGC9350.016.5872349
ATGGGTA91350.016.4631565
CTAGTAC61850.016.3367183
GTCCTAC50650.016.0531621
ATAAGGT45700.015.9397543
GTATTAG67500.015.52726651
ATTGCGT21850.015.486486
AATACGG28350.015.2512845
Produced by FastQC (version 0.11.5)