##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765354_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15594778 Sequences flagged as poor quality 0 Sequence length 100 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2462432616867 33.0 33.0 33.0 33.0 33.0 2 32.17587444976774 33.0 33.0 33.0 33.0 33.0 3 32.21104763402211 33.0 33.0 33.0 33.0 33.0 4 36.04550362948418 37.0 37.0 37.0 33.0 37.0 5 36.04385871988688 37.0 37.0 37.0 33.0 37.0 6 36.00248269003893 37.0 37.0 37.0 33.0 37.0 7 35.997425740847355 37.0 37.0 37.0 33.0 37.0 8 35.9763481083219 37.0 37.0 37.0 33.0 37.0 9 35.99041031555563 37.0 37.0 37.0 37.0 37.0 10-11 35.97649508059685 37.0 37.0 37.0 33.0 37.0 12-13 35.95673936493357 37.0 37.0 37.0 33.0 37.0 14-15 37.888857250805366 40.0 37.0 40.0 33.0 40.0 16-17 37.90867180026545 40.0 37.0 40.0 33.0 40.0 18-19 37.93290625874893 40.0 37.0 40.0 33.0 40.0 20-21 37.9467554138956 40.0 37.0 40.0 33.0 40.0 22-23 37.9462707003588 40.0 37.0 40.0 33.0 40.0 24-25 37.95940054420781 40.0 37.0 40.0 33.0 40.0 26-27 37.93836962603764 40.0 37.0 40.0 33.0 40.0 28-29 37.889013328692464 40.0 37.0 40.0 33.0 40.0 30-31 37.83364235771744 40.0 37.0 40.0 33.0 40.0 32-33 37.73881500589492 40.0 37.0 40.0 33.0 40.0 34-35 37.648115734638864 40.0 37.0 40.0 33.0 40.0 36-37 37.576288389613495 40.0 37.0 40.0 33.0 40.0 38-39 37.441689327029856 40.0 37.0 40.0 33.0 40.0 40-41 37.339556645179556 40.0 37.0 40.0 33.0 40.0 42-43 37.1870453686484 40.0 37.0 40.0 33.0 40.0 44-45 37.00199557185104 40.0 37.0 40.0 33.0 40.0 46-47 36.79557009404046 37.0 37.0 40.0 33.0 40.0 48-49 36.61390758496209 37.0 37.0 40.0 33.0 40.0 50-51 36.43324204422788 37.0 37.0 40.0 33.0 40.0 52-53 36.279904337208265 37.0 37.0 40.0 33.0 40.0 54-55 36.124909024033556 37.0 37.0 40.0 33.0 40.0 56-57 35.942294689927614 37.0 37.0 40.0 33.0 40.0 58-59 35.755021713037536 37.0 37.0 40.0 33.0 40.0 60-61 35.53788425202334 37.0 35.0 40.0 27.0 40.0 62-63 35.28810131186221 37.0 33.0 38.5 27.0 40.0 64-65 35.09072357426312 37.0 33.0 37.0 27.0 40.0 66-67 34.87559643362669 37.0 33.0 37.0 27.0 40.0 68-69 34.66300353874868 37.0 33.0 37.0 27.0 40.0 70-71 34.41133823771008 37.0 33.0 37.0 27.0 38.5 72-73 34.182941911709165 37.0 33.0 37.0 27.0 37.0 74-75 33.98200025675261 37.0 33.0 37.0 27.0 37.0 76-77 33.79668617276886 37.0 33.0 37.0 27.0 37.0 78-79 33.638514347559166 37.0 33.0 37.0 27.0 37.0 80-81 33.50944950931651 37.0 33.0 37.0 27.0 37.0 82-83 33.404910797704204 37.0 33.0 37.0 27.0 37.0 84-85 33.27739731210024 37.0 33.0 37.0 27.0 37.0 86-87 33.16452308586887 37.0 33.0 37.0 27.0 37.0 88-89 33.07193734979748 37.0 33.0 37.0 27.0 37.0 90-91 32.96106930794398 37.0 33.0 37.0 27.0 37.0 92-93 32.84297711708368 37.0 33.0 37.0 27.0 37.0 94-95 32.782636213224706 37.0 33.0 37.0 27.0 37.0 96-97 32.742786559706076 37.0 33.0 37.0 27.0 37.0 98-99 32.63263484738289 37.0 33.0 37.0 22.0 37.0 100 32.523996494211076 37.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 173.0 8 1421.0 9 3338.0 10 3998.0 11 5293.0 12 7680.0 13 12876.0 14 21844.0 15 28468.0 16 31781.0 17 37327.0 18 44006.0 19 51244.0 20 59724.0 21 72115.0 22 88347.0 23 90532.0 24 86536.0 25 89711.0 26 99940.0 27 116293.0 28 141441.0 29 180893.0 30 237125.0 31 314824.0 32 434711.0 33 609382.0 34 863925.0 35 1316229.0 36 2537290.0 37 5245829.0 38 2756699.0 39 3780.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56499681876075 17.85217512179776 12.09508189525104 25.48774616419045 2 16.05182190471714 20.81001415806879 36.889973933994376 26.248190003219698 3 18.673231513779807 25.924883316710247 28.56909537282288 26.832789796687074 4 13.12672742119189 17.041544291300585 35.422838337294706 34.408889950212824 5 14.28302943337892 37.29385749142962 33.23466875297446 15.188444322217004 6 33.04852432012819 35.24595861512104 17.385845441339402 14.319671623411375 7 28.10068375487423 31.133181188308644 22.141730849509162 18.62440420730796 8 27.743806292080592 32.32803314032428 20.19928081053799 19.72887975705714 9 27.998859618264525 14.188428972826673 18.89865312606566 38.914058282843136 10-11 25.90801587911023 25.04706646375588 26.50006308231914 22.54485457481475 12-13 27.0737390426462 22.80656063202695 26.221088238639883 23.898612086686967 14-15 25.271020209457294 23.58382081489073 23.799771308062223 27.345387667589755 16-17 24.604736277746305 25.725217120756703 25.61540472073408 24.054641880762905 18-19 25.220730939549124 25.097734639120866 26.646679420508583 23.034855000821427 20-21 25.647678344635622 24.772475119556045 25.683411459913057 23.89643507589528 22-23 24.651219369556777 24.628179366519827 24.92491960045698 25.795681663466418 24-25 24.442172641558134 25.491326625654516 24.782605350004953 25.2838953827824 26-27 25.19967601942242 24.64424593334608 24.908401936496368 25.247676110735128 28-29 24.618059081394918 24.50771355770545 26.04382969730698 24.83039766359265 30-31 25.52279359154712 24.575425183994284 25.441583714753747 24.46019750970485 32-33 24.035499250004104 24.923849382151122 25.322233402621602 25.71841796522317 34-35 24.484368070583763 25.0925696266461 25.937293119452782 24.48576918331736 36-37 25.391539080902852 24.77261689919599 25.18867621753868 24.64716780236248 38-39 25.71188014435618 24.1500318055552 25.064778169077307 25.073309881011312 40-41 24.817518201369314 25.00577677477799 25.41984496152862 24.756860062324083 42-43 26.052541434062093 24.428497795864743 24.94187156752087 24.57708920255229 44-45 25.629915988544372 24.553295981513813 24.786178424598283 25.030609605343535 46-47 24.71216647008377 24.684798334416815 26.165047043311553 24.437988152187867 48-49 25.759248590191223 24.96846420257757 25.17231067371839 24.099976533512816 50-51 24.280623090738903 25.52925475823181 25.09865043347068 25.0914717175586 52-53 24.19035594095743 25.447996333919594 24.257071704166908 26.104576020956067 54-55 24.846494283102146 25.320396523772143 24.274918318007423 25.558190875118285 56-57 25.180074210264298 24.23616930703047 24.38673867380005 26.197017808905187 58-59 24.892796458755456 24.265046994881374 25.25301600386161 25.589140542501553 60-61 25.075431332302678 24.773138500666285 24.833114208997483 25.318315958033548 62-63 23.876431036054377 24.993888075194644 25.04344396229841 26.086236926452568 64-65 23.815157944740758 24.909766657210383 25.211259459062923 26.063815938985936 66-67 25.082375463080435 24.675270586202934 24.852945731169964 25.389408219546667 68-69 25.47060304417286 24.139189413276675 24.515312112811095 25.874895429739365 70-71 24.208409105358882 24.65553071849402 24.920212734407006 26.21584744174009 72-73 25.356877221154512 24.501721712364287 24.750464085469318 25.390936981011887 74-75 25.71385789454193 24.243383720712124 24.72375704336615 25.319001341379792 76-77 24.851564715180572 24.568268495544864 25.583098252482394 24.997068536792163 78-79 25.071267292586754 24.67390893100023 25.32980080299549 24.925022973417526 80-81 24.268718526596793 24.594041756034894 25.65193864556518 25.48530107180313 82-83 24.26861524122299 25.090041310648413 24.40685498021036 26.234488467918236 84-85 24.4176036987518 25.150935108323797 24.18532875478135 26.246132438143054 86-87 24.62230435821839 24.197491043850864 24.69718861801573 26.483015979915013 88-89 24.605387136705193 23.89603866222524 25.231557460689253 26.267016740380317 90-91 25.074606929737996 24.291236572147348 24.83506448427998 25.799092013834674 92-93 24.145609040613458 24.68212084433806 24.82575390040362 26.346516214644865 94-95 23.44845355469973 24.891208703970584 25.523971918885955 26.13636582244373 96-97 24.759520832016115 24.37812838696844 24.869260052358822 25.993090728656625 98-99 24.643342149532362 24.25505191545529 24.65272349500583 26.44888244000652 100 24.040736361737107 24.784723806636364 24.919674210679606 26.254865620946923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 899.0 1 877.0 2 1221.5 3 1615.0 4 1339.5 5 1186.0 6 1562.0 7 3095.0 8 5075.5 9 4732.5 10 2826.0 11 1959.5 12 2081.0 13 2266.0 14 2626.0 15 3239.5 16 3881.5 17 4703.0 18 5708.5 19 6892.5 20 8477.0 21 10423.5 22 12776.5 23 15920.0 24 19853.0 25 25288.0 26 32956.0 27 41273.5 28 49810.0 29 63833.0 30 80836.5 31 95548.5 32 114817.5 33 132959.5 34 151194.0 35 168474.5 36 181750.5 37 203283.0 38 215966.0 39 222201.5 40 235708.0 41 247363.5 42 263036.5 43 305332.5 44 360544.0 45 403930.5 46 467868.5 47 546417.0 48 647991.0 49 778533.5 50 994712.5 51 989397.0 52 762716.0 53 690859.0 54 672293.5 55 643377.0 56 622440.5 57 597935.5 58 586958.0 59 548151.0 60 471039.5 61 389143.5 62 319580.0 63 253334.0 64 183804.5 65 142041.0 66 109720.5 67 78082.0 68 59566.0 69 50397.5 70 45946.5 71 42831.0 72 38023.5 73 41335.5 74 32563.5 75 21397.5 76 15653.5 77 10602.0 78 7372.5 79 3869.0 80 2281.0 81 1637.0 82 1220.0 83 881.5 84 630.5 85 434.5 86 306.5 87 190.0 88 98.0 89 68.5 90 53.0 91 34.0 92 20.0 93 24.0 94 22.5 95 12.0 96 6.0 97 2.5 98 1.0 99 1.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0820787573891722 2 8.784992001809836E-4 3 0.0 4 0.0 5 0.06405349277815946 6 0.0 7 5.129922336823262E-5 8 0.0 9 0.0 10-11 4.841364205376954E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010388092732067106 24-25 0.0013658418221791935 26-27 1.0259844673646524E-4 28-29 0.0037159874927363504 30-31 0.0 32-33 1.6672247594675603E-4 34-35 1.9237208763087235E-4 36-37 4.168061898668901E-5 38-39 1.1542325257852341E-4 40-41 2.2443410223601773E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.001272861979824272 50-51 4.2963099570894825E-4 52-53 0.006489351756081427 54-55 3.2062014605145387E-6 56-57 0.0019525766894533541 58-59 0.0016704309609280746 60-61 8.400247826548092E-4 62-63 0.001689668169691162 64-65 0.0013466046134161063 66-67 1.6031007302572695E-4 68-69 0.0 70-71 1.7634108032829965E-4 72-73 8.27199976812751E-4 74-75 0.004876632421442614 76-77 0.006601568807199436 78-79 0.0042834851512474235 80-81 0.006771497484606707 82-83 8.368185811942946E-4 84-85 0.002103268158097538 86-87 2.9176433290682306E-4 88-89 0.0034530789729741588 90-91 3.0138293728836665E-4 92-93 0.0033761301379218096 94-95 0.0015389767010469788 96-97 5.129922336823262E-4 98-99 0.0 100 0.0010259844673646524 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.5594778E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.388737518689855 #Duplication Level Percentage of deduplicated Percentage of total 1 70.34987117189478 19.971440271725672 2 14.706857840821854 8.350182539355549 3 5.5590172859542415 4.734404477784438 4 2.6982682355589387 3.0640171477720446 5 1.544093373622003 2.191743074405168 6 1.0149953700728302 1.728866228620983 7 0.664092908879402 1.3196931494740392 8 0.48130330120409504 1.0930874467809581 9 0.37711663406749873 0.9635278624626629 >10 2.131976036329108 11.27566105478756 >50 0.21553776759481538 4.285386270740977 >100 0.20056844089604858 11.95919678566658 >500 0.03142878227273068 6.208638331209171 >1k 0.021732690255334194 11.961324485166193 >5k 0.002168743995396614 4.2692018386816555 >10k+ 9.714165812714E-4 6.623629035366559 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 150801 0.966996772894106 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 138180 0.886065835627798 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 61180 0.392310810708559 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 41697 0.26737796459814944 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 40875 0.26210696939706357 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 39195 0.2513341324897347 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 37906 0.24306854512452822 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 36225 0.23228929581427835 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 26236 0.1682358030361189 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 22638 0.14516397732625627 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 22364 0.1434069789258943 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 18656 0.11962978889471848 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 18253 0.11704559051754376 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17804 0.1141664216060017 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 16292 0.10447086838940574 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 15910 0.10202133047357263 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2824805842058155E-5 0.0 0.0 0.0 2.564961168411631E-5 2 2.629085197621922E-4 3.847441752617447E-5 0.0 0.0 2.564961168411631E-5 3 2.629085197621922E-4 3.847441752617447E-5 0.0 1.2824805842058155E-5 2.564961168411631E-5 4 2.629085197621922E-4 1.0259844673646524E-4 0.0 1.9237208763087233E-5 2.564961168411631E-5 5 2.629085197621922E-4 1.154232525785234E-4 0.0 1.9237208763087233E-5 3.2062014605145387E-5 6 2.629085197621922E-4 1.154232525785234E-4 0.0 1.9237208763087233E-5 4.488682044720355E-5 7 2.6932092268322125E-4 1.154232525785234E-4 0.0 1.9237208763087233E-5 4.488682044720355E-5 8 2.6932092268322125E-4 1.474852671836688E-4 0.0 1.9237208763087233E-5 4.488682044720355E-5 9 2.6932092268322125E-4 2.821457285252794E-4 0.0 1.9237208763087233E-5 4.488682044720355E-5 10-11 2.6932092268322125E-4 2.821457285252794E-4 0.0 5.129922336823263E-5 4.488682044720355E-5 12-13 2.725271241437358E-4 2.821457285252794E-4 0.0 7.694883505234893E-5 9.618604381543617E-5 14-15 2.91764332906823E-4 4.4886820447203544E-4 0.0 1.6672247594675603E-4 1.1542325257852339E-4 16-17 3.174139445909393E-4 4.7131161469563725E-4 0.0 2.3725890807807585E-4 1.314542598810961E-4 18-19 3.3985735481454113E-4 5.097860322218117E-4 0.0 3.2382634751196846E-4 1.474852671836688E-4 20-21 3.751255708802011E-4 5.514666512085007E-4 0.0 5.322294424454135E-4 1.6672247594675603E-4 22-23 3.847441752617447E-4 5.899410687346751E-4 0.0 0.001195913144771923 1.7634108032829965E-4 24-25 4.4245580155100636E-4 6.380340906423933E-4 0.0 0.0026194665932403782 1.8595968470984327E-4 26-27 5.482604497479861E-4 6.700961052475386E-4 0.0 0.004642579714825052 2.0199069201241594E-4 28-29 5.70703859971588E-4 6.765085081685677E-4 0.0 0.016002151489428063 2.1160929639395956E-4 30-31 6.476526950239369E-4 7.342201344578295E-4 0.0 0.051331285382837766 2.1160929639395956E-4 32-33 7.406325373788585E-4 7.374263359183439E-4 0.0 0.1145832277958686 2.1160929639395956E-4 34-35 7.630759476024603E-4 8.496433870363528E-4 0.0 0.19378602247495924 2.308465051570468E-4 36-37 7.983441636681202E-4 9.682728410753907E-4 0.0 0.3136883384938215 2.308465051570468E-4 38-39 8.528495884968674E-4 9.93922452759507E-4 0.0 0.514717170068083 2.468775124596195E-4 40-41 8.560557899573819E-4 0.0011734697345483212 0.0 0.783868164073897 2.50083713920134E-4 42-43 9.137674162466436E-4 0.0012119441520744957 0.0 0.9933966357199826 2.50083713920134E-4 44-45 9.586542366938471E-4 0.0013562232177976499 0.0 1.2242239036682663 2.629085197621922E-4 46-47 9.650666396148761E-4 0.0014556154630736007 0.0 1.4842404297130745 2.6932092268322125E-4 48-49 9.971286542200216E-4 0.0014620278659946296 0.0 1.7451611045697477 2.7573332560425033E-4 50-51 0.001022778265904138 0.0016864619682306473 0.0 2.022045456498323 2.885581314463085E-4 52-53 0.0010323968702856815 0.0016928743711516765 0.0 2.312395854561059 3.0138293728836665E-4 54-55 0.0010740774892723706 0.0016960805726121912 0.0 2.585041608158834 3.0779534020939573E-4 56-57 0.0011510263243247195 0.0017185239828357928 0.0 2.8863379780077665 3.142077431304248E-4 58-59 0.0011927069433114086 0.0017217301842963073 0.0 3.2270193265976594 3.206201460514539E-4 60-61 0.0012792743827453011 0.0017569984003619674 0.0 3.5631286319048594 3.206201460514539E-4 62-63 0.0013722542251002226 0.0018147100266512292 0.0 3.9044993138087634 3.4306355627505565E-4 64-65 0.0014011100382448536 0.0020519689347293047 0.0 4.2512980947853185 3.526821606565993E-4 66-67 0.001439584455771028 0.002074412344952907 0.0 4.62063005962637 3.783317723407156E-4 68-69 0.0014652340674551443 0.0021321239712421684 0.0 5.05482668621509 3.911565781827738E-4 70-71 0.0014716464703761734 0.002177010791689372 0.0 5.582429579952981 3.911565781827738E-4 72-73 0.0015261518952049205 0.002196248000452459 0.0 6.056367073644781 4.0077518256431737E-4 74-75 0.0015678325141916096 0.0022379286194391486 0.0 6.498572791481867 4.1039378694586096E-4 76-77 0.0015806573200336676 0.0023148774544914973 0.0 6.975815878879455 4.1039378694586096E-4 78-79 0.0015966883273362403 0.0023469394690966423 0.0 7.468227505386739 4.1039378694586096E-4 80-81 0.0016191317375598422 0.0023597642749387006 3.2062014605145387E-6 8.01215317076011 4.1359998840637547E-4 82-83 0.001635162744862415 0.002369382879320244 6.4124029210290775E-6 8.61546089338367 4.296309957089482E-4 84-85 0.0016511937521649874 0.0024046510953859043 6.4124029210290775E-6 9.182301280595338 4.296309957089482E-4 86-87 0.0016832557667701329 0.0024399193114515642 6.4124029210290775E-6 9.750202920490436 4.4245580155100636E-4 88 0.0016928743711516765 0.0027124464355952997 6.4124029210290775E-6 10.203960582189756 4.616930103140936E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACT 4985 0.0 19.89248 4 CATGGGG 38280 0.0 19.213835 4 CGTTAAC 985 0.0 18.131815 1 GTATAAG 5915 0.0 18.116488 1 GTATTAC 3450 0.0 17.982445 1 GTACAAG 5750 0.0 17.328539 1 GTACATA 3330 0.0 17.219063 1 GTACTAG 4225 0.0 17.131207 1 CATGGGT 11800 0.0 16.926945 4 CTATTGA 5330 0.0 16.841385 9 ATTTAGG 5730 0.0 16.732832 1 TTTAGGG 6080 0.0 16.619116 2 TATTCGC 935 0.0 16.587234 9 ATGGGTA 9135 0.0 16.463156 5 CTAGTAC 6185 0.0 16.336718 3 GTCCTAC 5065 0.0 16.053162 1 ATAAGGT 4570 0.0 15.939754 3 GTATTAG 6750 0.0 15.5272665 1 ATTGCGT 2185 0.0 15.48648 6 AATACGG 2835 0.0 15.251284 5 >>END_MODULE