FastQCFastQC Report
Fri 27 May 2016
ERR765353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13215698
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1292360.9778976486902168No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT994500.7525141691343129No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG563840.4266441318498652No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT538250.40728079591407124No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG458600.34701156155354035No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG452380.3423050375394474No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA367810.2783129578172867No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC359280.27185851250535537No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC335010.2534939887397548No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC297720.22527754493179247No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC287720.21771078606669128No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT279070.21116553964837878No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC178280.13490017704702392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174270.13186590674211834No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG170930.12933860928117455No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA149590.11319114586304863No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG142320.10769011216812006No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT141240.10687290221068914No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG133220.100804361600878No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC75900.020.8370841
GTACTAG33750.019.525121
GTATAAG46200.018.7462881
TCTACAC111150.018.5897313
TACACTC91650.018.1796515
CTACACT123150.017.7333224
GTATATA39050.017.2367131
CATGGGG255100.016.2728884
GTATTAG39300.015.8095981
CTAACGC14050.015.0748923
GTATAAT43850.015.0278861
ATCTACA113650.014.6604932
GTATTAT42150.014.4056071
TAGGCAT57750.014.262675
GTATAAA97000.014.1693711
GTGATCG28050.014.0950358
TCTAACG15100.014.0265322
GTCCTAC40250.013.9161961
TAGTACT46750.013.8932554
CTAGGCA64100.013.8040454
Produced by FastQC (version 0.11.5)