##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765353_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13215698
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77121200862792	33.0	33.0	33.0	27.0	33.0
2	31.729492229619655	33.0	33.0	33.0	27.0	33.0
3	31.772928149538526	33.0	33.0	33.0	27.0	33.0
4	35.518608551739	37.0	37.0	37.0	33.0	37.0
5	35.47323251484712	37.0	37.0	37.0	33.0	37.0
6	35.48158886499979	37.0	37.0	37.0	33.0	37.0
7	35.34805433659274	37.0	37.0	37.0	33.0	37.0
8	35.327601614383134	37.0	37.0	37.0	33.0	37.0
9	35.170971294894905	37.0	37.0	37.0	33.0	37.0
10-11	35.25439753541583	37.0	37.0	37.0	33.0	37.0
12-13	35.25738614033099	37.0	37.0	37.0	33.0	37.0
14-15	36.69959649501676	40.0	37.0	40.0	33.0	40.0
16-17	36.833276380861605	40.0	37.0	40.0	33.0	40.0
18-19	36.89002048926966	40.0	37.0	40.0	33.0	40.0
20-21	36.88795907715203	40.0	37.0	40.0	33.0	40.0
22-23	36.824138271016785	40.0	37.0	40.0	33.0	40.0
24-25	36.77234516860176	40.0	37.0	40.0	33.0	40.0
26-27	36.748385140156806	40.0	37.0	40.0	33.0	40.0
28-29	36.67464631834051	37.0	37.0	40.0	33.0	40.0
30-31	36.618228564242315	37.0	37.0	40.0	33.0	40.0
32-33	36.47675756513201	37.0	37.0	40.0	33.0	40.0
34-35	36.42476375443809	37.0	37.0	40.0	33.0	40.0
36-37	36.35535845325764	37.0	37.0	40.0	33.0	40.0
38-39	36.25701843368394	37.0	37.0	40.0	33.0	40.0
40-41	36.161936395641	37.0	37.0	40.0	33.0	40.0
42-43	36.03630315250848	37.0	37.0	40.0	33.0	40.0
44-45	35.83255227987201	37.0	37.0	40.0	33.0	40.0
46-47	35.63106239261823	37.0	37.0	40.0	30.0	40.0
48-49	35.405762601415375	37.0	37.0	40.0	27.0	40.0
50-51	35.24706405972655	37.0	37.0	40.0	27.0	40.0
52-53	35.06330717454348	37.0	33.0	40.0	27.0	40.0
54-55	34.87679375693966	37.0	33.0	40.0	27.0	40.0
56-57	34.70934130758738	37.0	33.0	40.0	27.0	40.0
58-59	34.49361619038207	37.0	33.0	40.0	27.0	40.0
60-61	34.249790589948404	37.0	33.0	38.5	27.0	40.0
62-63	33.96422841230179	37.0	33.0	37.0	27.0	40.0
64-65	33.71340454359656	37.0	33.0	37.0	27.0	40.0
66-67	33.48256754959141	37.0	33.0	37.0	27.0	40.0
68-69	33.21117246323274	37.0	33.0	37.0	27.0	40.0
70-71	32.90755104270694	37.0	33.0	37.0	27.0	38.5
72-73	32.605554356644646	37.0	33.0	37.0	27.0	37.0
74-75	32.31882504427689	37.0	33.0	37.0	22.0	37.0
76-77	32.048167149400655	35.0	33.0	37.0	22.0	37.0
78-79	31.847354714068075	33.0	33.0	37.0	22.0	37.0
80-81	31.614602989565896	33.0	33.0	37.0	22.0	37.0
82-83	31.438567338630165	33.0	33.0	37.0	22.0	37.0
84-85	31.22429632547596	33.0	33.0	37.0	22.0	37.0
86-87	31.009388872233615	33.0	33.0	37.0	22.0	37.0
88-89	30.805148505966162	33.0	33.0	37.0	15.0	37.0
90-91	30.60542409489079	33.0	33.0	37.0	15.0	37.0
92-93	30.392188063014153	33.0	33.0	37.0	15.0	37.0
94-95	30.134575222587564	33.0	33.0	37.0	10.5	37.0
96-97	29.888461320771704	33.0	30.0	37.0	4.0	37.0
98-99	29.559262666262498	33.0	27.0	37.0	2.0	37.0
100	29.237257615904962	33.0	27.0	37.0	2.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105136.0
3	25034.0
4	14408.0
5	11161.0
6	12237.0
7	16540.0
8	20993.0
9	20898.0
10	21720.0
11	24991.0
12	29588.0
13	33021.0
14	36581.0
15	41559.0
16	47874.0
17	52836.0
18	50314.0
19	41669.0
20	39122.0
21	42129.0
22	49424.0
23	61831.0
24	77702.0
25	96490.0
26	117221.0
27	143189.0
28	179468.0
29	230420.0
30	299007.0
31	394769.0
32	533801.0
33	738710.0
34	1036799.0
35	1528570.0
36	2464244.0
37	3416261.0
38	1159246.0
39	735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88175889787942	18.22002682654125	12.567020002610763	25.33119427296857
2	15.054042657246832	21.34391600884548	37.32564363264166	26.27639770126603
3	18.261099181689815	26.371130605309705	29.439173023108488	25.928597189891995
4	12.961900738111549	17.834204586222317	36.240610087224205	32.96328458844193
5	13.745275085354214	37.64668865032243	33.70404153191927	14.903994732404083
6	31.708550867401648	35.89282611341217	17.988571477007635	14.41005154217855
7	27.122972055304086	31.9658823286346	22.21986605732307	18.691279558738245
8	27.172941997764628	33.20378675317536	20.592563025769756	19.03070822329026
9	27.54605491568744	15.018665328376898	19.249389854282402	38.185889901653255
10-11	24.510387892373373	26.113213350360127	27.600223608175146	21.776175149091355
12-13	26.317554137018632	23.610619941945824	26.98957195717422	23.08225396386132
14-15	24.49112765276689	23.9112754682119	24.87084462033449	26.726752258686716
16-17	23.693963446515898	26.743211144201535	26.262049888683524	23.300775520599043
18-19	24.47876352631454	26.24017811531352	27.17921759989721	22.10184075847473
20-21	24.481303072928824	25.865391629896074	26.691693221103947	22.961612076071162
22-23	23.8330703828734	26.27535198436941	25.332014765587502	24.55956286716969
24-25	23.81889704198749	26.650211740613322	25.113558133668008	24.41733308373118
26-27	24.34245622138157	25.73865186689345	25.235208915942238	24.683682995782743
28-29	24.07450194679707	25.432116383858073	26.526717521150918	23.966664148193946
30-31	25.083586201803342	25.42398441610878	26.087229747532064	23.405199634555814
32-33	23.278229420799416	26.041704342820182	26.37053676620032	24.309529470180085
34-35	23.179664819822605	26.217832005543713	26.53635850334958	24.066144671284103
36-37	24.452728282958752	25.98427869049319	25.740784081470412	23.822208945077644
38-39	24.870317103190466	25.340977071358623	25.69953929031974	24.089166535131177
40-41	24.017059106526194	25.96206042238556	25.827273746721513	24.193606724366735
42-43	25.038032424623534	25.533991888585216	25.469954403464307	23.958021283326943
44-45	25.019194105955698	25.323893562368937	25.81892802738996	23.8379843042854
46-47	24.27004366344325	25.455279665632858	26.801678888223492	23.472997782700403
48-49	24.845759838500957	25.678358757933534	26.37080074503678	23.10508065852873
50-51	23.875215915193827	26.131535602289652	25.931748192800942	24.06150028971558
52-53	23.514010093658403	26.646435663871255	24.846927199376218	24.992627043094124
54-55	24.013787642518352	26.21056934544302	24.783828861971248	24.99181415006738
56-57	24.541386768983127	25.252138714431567	25.077064245731425	25.129410270853885
58-59	24.282908466441004	25.210665101441943	26.228588397761627	24.27783803435543
60-61	24.6218080318656	25.57385022138708	25.687057050377156	24.117284696370167
62-63	23.199415432308587	25.927849491299288	26.094819646050965	24.77791543034116
64-65	23.07943820216217	25.88137502903803	26.02906980703617	25.010116961763636
66-67	24.354419925967985	25.682678561084927	25.428745650419465	24.534155862527623
68-69	24.65807853714588	25.076015021439176	25.473002849009745	24.7929035924052
70-71	23.780156388838716	25.681092165810398	25.57577384205649	24.962977603294398
72-73	24.662985090110574	25.348034063770502	25.43780659022205	24.551174255896875
74-75	24.877765580577787	25.020164474922524	25.6844353786228	24.41763456587689
76-77	24.124983788219133	25.498422406444217	26.286307389893445	24.09028641544321
78-79	24.403024181854978	25.44630812799665	26.094572478655742	24.056095211492625
80-81	23.8025912147554	25.59359446403626	25.973181051754867	24.630633269453472
82-83	23.660099047367687	26.067471129218532	25.037014695479172	25.23541512793461
84-85	23.985842402655546	25.935260670475003	24.910014902395535	25.16888202447392
86-87	24.08942817088702	25.100760997599895	25.313644497569893	25.496166333943187
88-89	24.14150816438511	24.75751213815517	25.936582894469566	25.164396802990147
90-91	24.507402530308443	25.1933768337491	25.646073489382704	24.653147146559753
92-93	23.437285528509964	25.438401480187167	25.968380158457876	25.155932832844996
94-95	22.958295231367998	25.636463930791066	26.17081963901091	25.23442119883002
96-97	24.042629374388415	25.321811006867907	25.45693084485425	25.178628773889432
98-99	24.25492316553574	25.02450875792732	25.30150917652087	25.419058900016072
100	23.846808138417906	25.375710642101218	25.656301385421525	25.121179834059355
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2796.0
1	2534.5
2	2478.5
3	2582.0
4	2067.5
5	1931.0
6	2646.0
7	4770.5
8	7749.5
9	7391.5
10	4280.5
11	2662.0
12	2548.0
13	2703.5
14	3002.0
15	3502.0
16	4057.0
17	4644.5
18	5482.5
19	6658.0
20	8115.5
21	10061.0
22	13235.0
23	17209.5
24	21675.0
25	27779.0
26	36986.5
27	47737.5
28	57954.0
29	71505.5
30	87060.5
31	101061.0
32	117667.0
33	137367.0
34	158791.0
35	177116.0
36	197132.5
37	222125.5
38	239245.5
39	253505.5
40	269690.5
41	284522.5
42	299996.5
43	325784.0
44	355072.0
45	384102.0
46	426377.0
47	475765.5
48	576742.0
49	688547.0
50	842178.5
51	835418.5
52	650585.5
53	572843.0
54	537792.5
55	496271.5
56	455583.5
57	417735.0
58	389594.0
59	356184.0
60	305687.5
61	254095.5
62	206587.5
63	164770.5
64	125165.0
65	93906.0
66	72098.5
67	55493.5
68	42938.0
69	34167.0
70	28569.0
71	23787.0
72	18900.0
73	17150.5
74	13818.0
75	10178.0
76	7879.0
77	5853.0
78	4402.5
79	3066.5
80	2142.5
81	1615.5
82	1244.0
83	934.0
84	681.5
85	453.5
86	318.5
87	198.5
88	113.0
89	69.5
90	58.5
91	42.0
92	22.5
93	13.5
94	10.5
95	10.5
96	11.0
97	12.5
98	6.5
99	7.0
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00887580814876369
2	0.05060648328979672
3	0.04938066835365033
4	0.04673986950973002
5	0.07894399523960066
6	0.018478025148577094
7	0.01615503017699103
8	0.02035458134712219
9	0.006219875787113174
10-11	0.018209405208866002
12-13	0.03473142319081444
14-15	0.010457260751569837
16-17	0.006791166081428313
18-19	0.00332180714177942
20-21	0.01009405632604498
22-23	0.014115788662846261
24-25	0.0
26-27	0.0
28-29	1.2485152127416957E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	3.7833794325505926E-5
38-39	0.0
40-41	0.0
42-43	7.566758865101185E-6
44-45	3.972548404178122E-4
46-47	0.003964981645313021
48-49	0.003870397159499256
50-51	0.0011387972091977284
52-53	0.0013052659042299543
54-55	0.006212309028248072
56-57	0.005675069148825889
58-59	0.013877435758595572
60-61	0.007169504024683372
62-63	0.003495842595676748
64-65	0.0020278913758471177
66-67	0.0015095683935876865
68-69	4.048215992829134E-4
70-71	3.7077118438995805E-4
72-73	5.561567765849371E-4
74-75	4.161717375805652E-5
76-77	0.0
78-79	1.8538559219497902E-4
80-81	3.405041489295533E-5
82-83	7.566758865101185E-6
84-85	2.8375345744129446E-4
86-87	1.4376841843692252E-4
88-89	2.7618669857619325E-4
90-91	8.73960648919187E-4
92-93	5.448066382872853E-4
94-95	4.7670580850137464E-4
96-97	1.6646869503222607E-4
98-99	1.0593462411141659E-4
100	6.810082978591067E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3215698E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.212376663347975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57783563775905	26.635813804995017
2	14.024221360235284	10.437492153344914
3	5.352049629850872	5.974874602408282
4	2.577009600983018	3.8358660774737654
5	1.4761909689342803	2.7466287181507525
6	0.963032535619954	2.150203767272928
7	0.6651966249511054	1.7327483154007846
8	0.48077890530172374	1.431274057270388
9	0.37778058038463747	1.2652301928034202
>10	2.171766458009855	14.963715643735597
>50	0.18448379748686755	4.749123244696455
>100	0.12980206766561508	9.535101224471077
>500	0.012725336581404786	3.2299263972589554
>1k	0.006225450133880791	4.105834114768447
>5k	3.8908808985298594E-4	1.1187230639537347
>10k+	5.119580129644552E-4	6.087444621995395
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	129236	0.9778976486902168	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	99450	0.7525141691343129	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	56384	0.4266441318498652	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	53825	0.40728079591407124	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	45860	0.34701156155354035	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	45238	0.3423050375394474	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	36781	0.2783129578172867	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	35928	0.27185851250535537	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	33501	0.2534939887397548	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	29772	0.22527754493179247	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	28772	0.21771078606669128	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	27907	0.21116553964837878	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	17828	0.13490017704702392	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17427	0.13186590674211834	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	17093	0.12933860928117455	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	14959	0.11319114586304863	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	14232	0.10769011216812006	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	14124	0.10687290221068914	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	13322	0.100804361600878	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1023711346914856E-4	0.0	0.0	3.026703546040474E-5	7.566758865101185E-6
2	4.3887201417586874E-4	0.0	0.0	5.2967312055708296E-5	7.566758865101185E-6
3	4.540055319060711E-4	0.0	0.0	5.2967312055708296E-5	7.566758865101185E-6
4	5.296731205570829E-4	0.0	0.0	5.2967312055708296E-5	7.566758865101185E-6
5	0.0020505916524424213	0.0	0.0	1.8916897162752963E-4	7.566758865101185E-6
6	0.004767058085013746	0.0	0.0	4.464387730409699E-4	2.2700276595303556E-5
7	0.005720469702016496	0.0	0.0	4.994060850966782E-4	2.2700276595303556E-5
8	0.006885750567242078	0.0	0.0	6.280409858033984E-4	2.2700276595303556E-5
9	0.007763494595593816	7.566758865101185E-6	0.0	6.734415389940055E-4	3.026703546040474E-5
10-11	0.017078174758533377	7.566758865101185E-6	0.0	0.0013393163191229096	3.026703546040474E-5
12-13	0.028590998371784825	1.513351773020237E-5	0.0	0.002451629872292784	3.026703546040474E-5
14-15	0.04134098705948032	2.6483656027854148E-5	0.0	0.003582860322625411	3.026703546040474E-5
16-17	0.047182524903338435	3.026703546040474E-5	0.0	0.004093616546019741	3.7833794325505926E-5
18-19	0.050088160307537294	3.026703546040474E-5	0.0	0.004369803244595934	4.540055319060711E-5
20-21	0.05866508148112949	3.026703546040474E-5	0.0	0.005232413755217469	4.540055319060711E-5
22-23	0.06113562825058502	3.7833794325505926E-5	0.0	0.0064317450353360076	4.540055319060711E-5
24-25	0.07165720645250823	4.161717375805652E-5	0.0	0.008667722279973408	6.053407092080948E-5
26-27	0.09845488297326407	4.540055319060711E-5	0.0	0.013207777599034118	6.431745035336007E-5
28-29	0.1047617764873259	4.540055319060711E-5	0.0	0.0229953801910425	7.945096808356244E-5
30-31	0.10808358362910533	4.540055319060711E-5	0.0	0.04205604577223239	9.458448581376481E-5
32-33	0.11115190434890385	4.540055319060711E-5	0.0	0.06823703144548249	1.1728476240906837E-4
34-35	0.11347111594105737	4.9183932623157704E-5	0.0	0.10344894382423084	1.2106814184161896E-4
36-37	0.1210265246678609	5.675069148825889E-5	0.0	0.15742641818843017	1.399850390043719E-4
38-39	0.12868786801877585	6.810082978591067E-5	0.0	0.24956305750933475	1.513351773020237E-4
40-41	0.13440833772079236	7.188420921846126E-5	0.0	0.39091389648885744	1.589019361671249E-4
42-43	0.14696915743686031	7.566758865101185E-5	0.0	0.501520994199474	1.6646869503222607E-4
44-45	0.1602790862805733	7.945096808356244E-5	0.0	0.6196645837397314	1.6646869503222607E-4
46-47	0.16412678316347723	9.080110638121422E-5	0.0	0.7539253696626542	1.8538559219497902E-4
48-49	0.166688131039314	9.080110638121422E-5	0.0	0.8748194760503758	2.04302489357732E-4
50-51	0.17148923953922068	9.080110638121422E-5	0.0	1.0143694264200045	2.04302489357732E-4
52-53	0.172858822893804	9.080110638121422E-5	0.0	1.185533295328026	2.2321938652048498E-4
54-55	0.17834472307100238	9.836786524631541E-5	0.0	1.326399861740182	2.4213628368323793E-4
56-57	0.1890025029324974	9.836786524631541E-5	0.0	1.5011730746268568	2.459196631157885E-4
58-59	0.19235079373030467	9.836786524631541E-5	0.0	1.6975493840733953	2.610531808459909E-4
60-61	0.19542289782953576	9.836786524631541E-5	0.0	1.8661973056587704	2.6483656027854145E-4
62-63	0.19847608503160408	9.836786524631541E-5	0.0	2.049672291240311	2.7618669857619325E-4
64-65	0.20052289330461395	1.0593462411141659E-4	0.0	2.241281542601836	2.799700780087438E-4
66-67	0.20390145113788163	1.0593462411141659E-4	0.0	2.4559845420196496	2.8375345744129446E-4
68-69	0.2074578278044792	1.0593462411141659E-4	0.0	2.72707881187963	2.8753683687384504E-4
70-71	0.21183141442850767	1.0593462411141659E-4	0.0	2.998131464565852	2.913202163063956E-4
72-73	0.2241046973077018	1.0593462411141659E-4	0.0	3.2371994275292915	3.3293739006445215E-4
74-75	0.23712708931454093	1.0971800354396718E-4	0.0	3.5074954043290036	3.367207694970027E-4
76-77	0.2410807208215563	1.1350138297651778E-4	0.0	3.789417706124943	3.4428752836210394E-4
78-79	0.24357775124703968	1.1350138297651778E-4	0.0	4.063485712218908	3.480709077946545E-4
80-81	0.2492755206724609	1.1350138297651778E-4	0.0	4.354026552362198	3.5563766665975573E-4
82-83	0.25108775942065265	1.1350138297651778E-4	0.0	4.6922493234939235	3.632044255248569E-4
84-85	0.2581702457183873	1.2863490070672015E-4	0.0	5.051526601167793	3.632044255248569E-4
86-87	0.27066674798410195	1.3620165957182133E-4	0.0	5.379496414037306	3.632044255248569E-4
88	0.27353076621454275	1.513351773020237E-4	0.0	5.639316213188286	3.632044255248569E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	7590	0.0	20.837084	1
GTACTAG	3375	0.0	19.52512	1
GTATAAG	4620	0.0	18.746288	1
TCTACAC	11115	0.0	18.589731	3
TACACTC	9165	0.0	18.179651	5
CTACACT	12315	0.0	17.733322	4
GTATATA	3905	0.0	17.236713	1
CATGGGG	25510	0.0	16.272888	4
GTATTAG	3930	0.0	15.809598	1
CTAACGC	1405	0.0	15.074892	3
GTATAAT	4385	0.0	15.027886	1
ATCTACA	11365	0.0	14.660493	2
GTATTAT	4215	0.0	14.405607	1
TAGGCAT	5775	0.0	14.26267	5
GTATAAA	9700	0.0	14.169371	1
GTGATCG	2805	0.0	14.095035	8
TCTAACG	1510	0.0	14.026532	2
GTCCTAC	4025	0.0	13.916196	1
TAGTACT	4675	0.0	13.893255	4
CTAGGCA	6410	0.0	13.804045	4
>>END_MODULE