##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765349_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7979865
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5240859337846	34.0	31.0	34.0	31.0	34.0
2	32.66668120325344	34.0	31.0	34.0	31.0	34.0
3	32.718330573261575	34.0	31.0	34.0	31.0	34.0
4	36.207143103298115	37.0	35.0	37.0	35.0	37.0
5	36.14515947324924	37.0	35.0	37.0	35.0	37.0
6	36.19192881082575	37.0	37.0	37.0	35.0	37.0
7	36.15213340075302	37.0	37.0	37.0	35.0	37.0
8	36.156274573567345	37.0	37.0	37.0	35.0	37.0
9	37.90173455315347	39.0	38.0	39.0	35.0	39.0
10-11	37.85368442949849	39.0	38.0	39.0	35.0	39.0
12-13	37.819757477100175	39.0	38.0	39.0	35.0	39.0
14-15	39.22315433907717	41.0	39.0	41.0	36.0	41.0
16-17	39.19998778174819	40.0	39.0	41.0	36.0	41.0
18-19	39.215046996409086	41.0	39.0	41.0	36.0	41.0
20-21	39.18390618137023	40.5	39.0	41.0	36.0	41.0
22-23	39.113436380189384	40.0	39.0	41.0	35.5	41.0
24-25	39.0539505367572	40.0	39.0	41.0	35.0	41.0
26-27	38.930900522753205	40.0	38.0	41.0	35.0	41.0
28-29	38.812421262765724	40.0	38.0	41.0	35.0	41.0
30-31	38.67192565287759	40.0	38.0	41.0	35.0	41.0
32-33	38.46147553874658	40.0	38.0	41.0	34.0	41.0
34-35	38.3581687158868	40.0	38.0	41.0	34.0	41.0
36-37	38.24976550355175	40.0	38.0	41.0	34.0	41.0
38-39	38.10277460834237	40.0	38.0	41.0	33.5	41.0
40-41	37.9188592789477	40.0	37.0	41.0	33.0	41.0
42-43	37.70709504484098	40.0	37.0	41.0	33.0	41.0
44-45	37.45363367676019	40.0	37.0	41.0	33.0	41.0
46-47	37.16922335152286	40.0	36.0	41.0	32.0	41.0
48-49	36.904885045047756	39.5	36.0	41.0	31.5	41.0
50-51	36.64298788262709	39.0	35.5	41.0	31.0	41.0
52-53	36.51432731004847	39.0	35.0	41.0	31.0	41.0
54-55	36.51530852965558	39.0	35.0	41.0	31.0	41.0
56-57	36.3535203916357	39.0	35.0	41.0	31.0	41.0
58-59	36.22149553908494	39.0	35.0	41.0	31.0	41.0
60-61	35.928154548980466	38.0	35.0	40.5	31.0	41.0
62-63	35.60739824545904	37.0	35.0	40.0	30.0	41.0
64-65	35.363763046116695	37.0	35.0	40.0	30.0	41.0
66-67	35.02500819249448	36.5	35.0	39.0	30.0	41.0
68-69	34.69246077471235	36.0	35.0	39.0	30.0	41.0
70-71	34.32552693560605	35.0	34.0	38.5	29.5	40.5
72-73	33.96542360052457	35.0	34.0	37.0	29.0	39.5
74-75	33.6282811175377	35.0	34.0	37.0	29.0	39.0
76-77	32.18555370297618	34.5	32.0	35.5	26.5	37.5
78-79	32.969896232580375	35.0	34.0	36.0	29.0	37.0
80-81	32.93058867537233	35.0	34.0	35.5	29.0	37.0
82-83	32.770487595968106	35.0	34.0	35.0	29.0	37.0
84-85	32.59427352467742	35.0	34.0	35.0	29.0	36.0
86-87	32.43139783693083	35.0	34.0	35.0	29.0	36.0
88-89	32.29052064163993	35.0	34.0	35.0	29.0	36.0
90-91	32.168715072247466	35.0	34.0	35.0	28.0	35.5
92-93	32.02788919361418	35.0	34.0	35.0	27.0	35.0
94-95	31.928364514938536	35.0	34.0	35.0	27.0	35.0
96-97	31.818164718325434	35.0	33.0	35.0	27.0	35.0
98-99	31.691003857834687	35.0	33.0	35.0	27.0	35.0
100	31.359321868227095	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.42143559983019685
1101	2	0.36273690443995577
1101	3	0.412987979041624
1101	4	0.29261152198476026
1101	5	0.33200615388833654
1101	6	0.33896060888373825
1101	7	0.3178948724235866
1101	8	0.31638501415732634
1101	9	0.4003824120321653
1101	10-11	0.3997671743817932
1101	12-13	0.3817407993793367
1101	14-15	0.4815905356237735
1101	16-17	0.49221994490095256
1101	18-19	0.5034356118407786
1101	20-21	0.48027524969928237
1101	22-23	0.5019155902065577
1101	24-25	0.5098104039735745
1101	26-27	0.5138222268628709
1101	28-29	0.5393906947822558
1101	30-31	0.5757886417607097
1101	32-33	0.644147187753596
1101	34-35	0.6040725068589197
1101	36-37	0.6589269437057723
1101	38-39	0.6076489442435005
1101	40-41	0.6381607868876955
1101	42-43	0.6847467231440234
1101	44-45	0.729424450119609
1101	46-47	0.7581346572171199
1101	48-49	0.7508695341416569
1101	50-51	0.7608145471776098
1101	52-53	0.7670535348706196
1101	54-55	0.8861981456973425
1101	56-57	0.8790067624329154
1101	58-59	0.9046992286924365
1101	60-61	0.9734885673974674
1101	62-63	0.944914455122742
1101	64-65	0.9165822933710714
1101	66-67	0.8961842475361479
1101	68-69	0.930763675615701
1101	70-71	0.9269143685882568
1101	72-73	0.8722435309860685
1101	74-75	0.8208078924664335
1101	76-77	0.8350786230844349
1101	78-79	0.8236802752708812
1101	80-81	0.8204545946821966
1101	82-83	0.8222642955276953
1101	84-85	0.8332910660315704
1101	86-87	0.8426300911357529
1101	88-89	0.8393082109466548
1101	90-91	0.8438985424935623
1101	92-93	0.9270528010709356
1101	94-95	0.9604408514845382
1101	96-97	0.9914943300414656
1101	98-99	1.0339816662263566
1101	100	1.0624350547426467
1102	1	0.4009599366087713
1102	2	0.3366332420115725
1102	3	0.3936105703659365
1102	4	0.2580708261384572
1102	5	0.28280286774880636
1102	6	0.28092353308179696
1102	7	0.2927267707228438
1102	8	0.3041665373615672
1102	9	0.3525582458468932
1102	10-11	0.35776123658010306
1102	12-13	0.40572001030243854
1102	14-15	0.42155918950467
1102	16-17	0.5273334546055111
1102	18-19	0.47812622215284506
1102	20-21	0.5122144481114859
1102	22-23	0.4911342975723372
1102	24-25	0.48461730900521616
1102	26-27	0.584868631069341
1102	28-29	0.5737222383172806
1102	30-31	0.5732463607900513
1102	32-33	0.5882479747683078
1102	34-35	0.6107615914412889
1102	36-37	0.6163917639591645
1102	38-39	0.5777016878782675
1102	40-41	0.6478103739107723
1102	42-43	0.6572837310578805
1102	44-45	0.6645241327502447
1102	46-47	0.7117113731849045
1102	48-49	0.7469120925447115
1102	50-51	0.7534585557366142
1102	52-53	0.772495937683459
1102	54-55	0.8111194388803611
1102	56-57	0.7681131901572869
1102	58-59	0.775291501425059
1102	60-61	0.7904772896606076
1102	62-63	0.7494840417134228
1102	64-65	0.7628074606174948
1102	66-67	0.8056414434174357
1102	68-69	0.7896351511818693
1102	70-71	0.7907240458056677
1102	72-73	0.8864860704541115
1102	74-75	0.8379227927184658
1102	76-77	0.8671754548907913
1102	78-79	0.8283562733118188
1102	80-81	0.8164007555725519
1102	82-83	0.8002014410233116
1102	84-85	0.8272533981837853
1102	86-87	0.8435606983497976
1102	88-89	0.88788136739322
1102	90-91	0.8570614298194243
1102	92-93	0.8707425058522134
1102	94-95	0.938946178055911
1102	96-97	0.8872342608372001
1102	98-99	0.9077758331255517
1102	100	0.8878187352555109
1103	1	0.3617195707814247
1103	2	0.2690790230666522
1103	3	0.29079161106531615
1103	4	0.2517942943358946
1103	5	0.27866668145916407
1103	6	0.23917702231270255
1103	7	0.24124050111609563
1103	8	0.24621315724220239
1103	9	0.2999347867944451
1103	10-11	0.3405463245290363
1103	12-13	0.3438384748663097
1103	14-15	0.45099661930294843
1103	16-17	0.4460837469309098
1103	18-19	0.4183239493097588
1103	20-21	0.42144348392981357
1103	22-23	0.4555180686433218
1103	24-25	0.47153544183647966
1103	26-27	0.5281697196018769
1103	28-29	0.5494251980116331
1103	30-31	0.5319515145828788
1103	32-33	0.5407635090274496
1103	34-35	0.5787174916673976
1103	36-37	0.6300778284669164
1103	38-39	0.6224129890561514
1103	40-41	0.6605023575279887
1103	42-43	0.6457533700543294
1103	44-45	0.6049812005327411
1103	46-47	0.6111838959932641
1103	48-49	0.6068761827687226
1103	50-51	0.7031478705537921
1103	52-53	0.68195883382365
1103	54-55	0.6940604802499095
1103	56-57	0.6667480043500333
1103	58-59	0.6670417469783985
1103	60-61	0.7470134762294904
1103	62-63	0.7393976809360581
1103	64-65	0.7138517691204385
1103	66-67	0.678244113334415
1103	68-69	0.670489600007997
1103	70-71	0.5273826634887229
1103	72-73	0.6047461636212716
1103	74-75	0.6173680760350138
1103	76-77	0.5945114228177104
1103	78-79	0.6267306299341442
1103	80-81	0.5904644591242274
1103	82-83	0.5704420518804412
1103	84-85	0.5952918825175786
1103	86-87	0.5732373637668857
1103	88-89	0.5977587896800429
1103	90-91	0.6502971421754182
1103	92-93	0.6696159971913502
1103	94-95	0.7004959110711972
1103	96-97	0.7375629567552515
1103	98-99	0.7337104541566468
1103	100	0.7815609820877505
1104	1	0.29324462421568853
1104	2	0.22690973904541778
1104	3	0.23576739796322954
1104	4	0.21535524897208091
1104	5	0.21827186759963269
1104	6	0.18707656934469696
1104	7	0.20383854511291588
1104	8	0.17114900953856704
1104	9	0.26541864030895823
1104	10-11	0.2600863399227791
1104	12-13	0.2973159608762401
1104	14-15	0.3681128587126423
1104	16-17	0.3520714754468912
1104	18-19	0.2747411915370961
1104	20-21	0.3351848176427339
1104	22-23	0.3524613534968708
1104	24-25	0.37964622321693753
1104	26-27	0.39668805366642346
1104	28-29	0.37308044430280773
1104	30-31	0.41330857261213083
1104	32-33	0.3794054232873876
1104	34-35	0.43229779340605745
1104	36-37	0.41902158407046386
1104	38-39	0.4922663628923303
1104	40-41	0.5034546094424712
1104	42-43	0.5545742135556893
1104	44-45	0.4811425553510702
1104	46-47	0.5405818478208246
1104	48-49	0.585585592707254
1104	50-51	0.5898443367750517
1104	52-53	0.5417065876443772
1104	54-55	0.5491427131472832
1104	56-57	0.48016833470223474
1104	58-59	0.546023776285935
1104	60-61	0.6223611454791964
1104	62-63	0.6422108606858856
1104	64-65	0.5668000205934831
1104	66-67	0.5901219500161474
1104	68-69	0.5476819736307164
1104	70-71	0.5051519128632762
1104	72-73	0.517300840413732
1104	74-75	0.4811679206688453
1104	76-77	0.4429784878319154
1104	78-79	0.49605094981847486
1104	80-81	0.4729365651167683
1104	82-83	0.4464581491924733
1104	84-85	0.47456688246582956
1104	86-87	0.5210684373338097
1104	88-89	0.5594895910618973
1104	90-91	0.5671817915001469
1104	92-93	0.610351513013903
1104	94-95	0.6230375563931752
1104	96-97	0.6191663487854093
1104	98-99	0.6447083236049842
1104	100	0.6864412527637249
1105	1	0.22021128359396158
1105	2	0.11160093424123829
1105	3	0.18592397429691943
1105	4	0.13384465122424416
1105	5	0.1716618913200918
1105	6	0.18589244326593501
1105	7	0.1450089903240439
1105	8	0.13765718475435307
1105	9	0.15574356310420256
1105	10-11	0.19813667620641695
1105	12-13	0.22064650579893907
1105	14-15	0.241571909822774
1105	16-17	0.30226383711323734
1105	18-19	0.2759789408972111
1105	20-21	0.29239353032431126
1105	22-23	0.32548105483635226
1105	24-25	0.32464071971730846
1105	26-27	0.292245952109468
1105	28-29	0.3134040611705231
1105	30-31	0.2769104790620531
1105	32-33	0.2882854605654188
1105	34-35	0.3185077494911397
1105	36-37	0.3401396944181343
1105	38-39	0.28356309275495306
1105	40-41	0.2960628662750793
1105	42-43	0.30201678429811807
1105	44-45	0.3233344365236377
1105	46-47	0.36958927796482755
1105	48-49	0.23877421176039348
1105	50-51	0.29046922309915857
1105	52-53	0.24745503258745316
1105	54-55	0.3421086591535172
1105	56-57	0.3577527671121956
1105	58-59	0.30531891612706374
1105	60-61	0.3914317000745129
1105	62-63	0.3456651958655357
1105	64-65	0.3117193423603837
1105	66-67	0.30405812284449496
1105	68-69	0.27175737876190453
1105	70-71	0.25084485077926644
1105	72-73	0.2502902041082038
1105	74-75	0.28753105986474026
1105	76-77	0.23584889684914145
1105	78-79	0.29809892872051336
1105	80-81	0.27606591038087913
1105	82-83	0.33120196451302064
1105	84-85	0.32777920519802706
1105	86-87	0.36445779568784076
1105	88-89	0.39624585894863174
1105	90-91	0.40904166430864564
1105	92-93	0.38183885430813547
1105	94-95	0.4323086095318054
1105	96-97	0.4595389233442013
1105	98-99	0.48554634459802415
1105	100	0.5226597739824577
1106	1	0.21530413209158183
1106	2	0.12206329233777069
1106	3	0.14286930803009312
1106	4	0.11125488259240512
1106	5	0.16247717096649694
1106	6	0.13529433468175256
1106	7	0.13826874458317207
1106	8	0.1409769896421551
1106	9	0.1785397705466707
1106	10-11	0.19859156427760638
1106	12-13	0.16147639116849888
1106	14-15	0.27880983418236127
1106	16-17	0.24878802959403856
1106	18-19	0.259412521835543
1106	20-21	0.26476466901012685
1106	22-23	0.288654843017234
1106	24-25	0.2792003394349365
1106	26-27	0.2859823088443818
1106	28-29	0.3157480180145811
1106	30-31	0.27419806963442284
1106	32-33	0.284437502597477
1106	34-35	0.2681186293871818
1106	36-37	0.2775129707739481
1106	38-39	0.2746656576887858
1106	40-41	0.3287890574266825
1106	42-43	0.3492396706726808
1106	44-45	0.3471351124731257
1106	46-47	0.3308196607056928
1106	48-49	0.3596930518937427
1106	50-51	0.3137014915824139
1106	52-53	0.32400898021892743
1106	54-55	0.3520900414999488
1106	56-57	0.4085082629165626
1106	58-59	0.34180141222319094
1106	60-61	0.33713910336943087
1106	62-63	0.27231864815193063
1106	64-65	0.3433621603295052
1106	66-67	0.31065722495554837
1106	68-69	0.30411398601957984
1106	70-71	0.3229423980984265
1106	72-73	0.2841466080677222
1106	74-75	0.35092043865041234
1106	76-77	0.21902374542241176
1106	78-79	0.3427622845314673
1106	80-81	0.31712538421012226
1106	82-83	0.3122889038558725
1106	84-85	0.29915516225280925
1106	86-87	0.35042740295092045
1106	88-89	0.3480038308029876
1106	90-91	0.41199973693404246
1106	92-93	0.404899805257223
1106	94-95	0.39755963547832707
1106	96-97	0.41814228526085273
1106	98-99	0.4653702181506212
1106	100	0.5238613268832069
1107	1	0.152262320463592
1107	2	0.037790848738190164
1107	3	0.09759138578913706
1107	4	0.08247565121619971
1107	5	0.12126967302883429
1107	6	0.13908163606979684
1107	7	0.08884216375751919
1107	8	0.11642061612614185
1107	9	0.13670617419072784
1107	10-11	0.15134836998758772
1107	12-13	0.18115164044219512
1107	14-15	0.18143673511847425
1107	16-17	0.1983918144295913
1107	18-19	0.15087344056156127
1107	20-21	0.21076934065030883
1107	22-23	0.18880682824014627
1107	24-25	0.2035802935255333
1107	26-27	0.27074545399699446
1107	28-29	0.23085048474819558
1107	30-31	0.3269811028828684
1107	32-33	0.334802558549562
1107	34-35	0.2967650418185812
1107	36-37	0.25973708845265264
1107	38-39	0.28885901517237045
1107	40-41	0.34794692927489734
1107	42-43	0.34652881919677014
1107	44-45	0.35662499805829384
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2316	56-57	-0.6583646615750425
2316	58-59	-0.6883955805957456
2316	60-61	-0.5791099340759445
2316	62-63	-0.5704506302629824
2316	64-65	-0.6210077398856626
2316	66-67	-0.5539510767271167
2316	68-69	-0.5427377271772471
2316	70-71	-0.5072590753621355
2316	72-73	-0.5039929267577108
2316	74-75	-0.5457451367867989
2316	76-77	-0.574991809359986
2316	78-79	-0.6190423073554001
2316	80-81	-0.6209156767656978
2316	82-83	-0.6010605317963496
2316	84-85	-0.5944107867759101
2316	86-87	-0.5758561598748173
2316	88-89	-0.5635433215894636
2316	90-91	-0.5887025456739998
2316	92-93	-0.6176304086805651
2316	94-95	-0.5776543887920944
2316	96-97	-0.5661341844898722
2316	98-99	-0.5783241341604146
2316	100	-0.6438956501337962
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28.0
3	44.0
4	65.0
5	130.0
6	333.0
7	710.0
8	1609.0
9	2709.0
10	4426.0
11	7659.0
12	11896.0
13	16487.0
14	19451.0
15	25377.0
16	33368.0
17	44274.0
18	45443.0
19	29743.0
20	21857.0
21	19427.0
22	20495.0
23	23544.0
24	27643.0
25	33012.0
26	41758.0
27	53868.0
28	69792.0
29	89070.0
30	113163.0
31	143272.0
32	183134.0
33	243288.0
34	350551.0
35	574003.0
36	1112486.0
37	2151768.0
38	2069292.0
39	394290.0
40	400.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.5963640988914	18.581780518843363	13.289498005292069	24.532357376973167
2	15.903557561655557	21.9741766176104	36.22142682664417	25.900838994089874
3	19.37140434004312	26.12861590351382	29.369377477407127	25.130602279035934
4	14.236596176435828	18.052026810842893	34.761180448081404	32.950196564639874
5	14.542288622677201	36.69242524779555	33.08088545357597	15.684400675951284
6	32.24553297580849	34.256657224151034	17.763094989702207	15.734714810338271
7	26.806869539772915	31.713330989935294	22.113970599753255	19.365828870538536
8	27.459612411989426	31.732278678899956	21.067348883721717	19.7407600253889
9	27.762537336157944	14.657027405852105	19.712526465046714	37.86790879294324
10-11	25.538433969038284	25.288266516120906	26.381563324145613	22.791736190695193
12-13	27.13469497930538	23.20753567860657	26.404042330609638	23.25372701147841
14-15	25.334750155141112	23.41179613806406	24.600530437232262	26.652923269562567
16-17	23.954225841903902	26.226680267624747	26.336864319164693	23.482229571306657
18-19	24.8729270482646	25.427836185198622	27.454311570433838	22.24492519610294
20-21	24.98911447363021	25.124283605792623	26.733561977948234	23.153039942628936
22-23	24.097877623394872	25.4927251161715	25.352083022509813	25.057314237923812
24-25	24.135138423843294	26.11743046789501	25.089472760167137	24.657958348094557
26-27	24.710516834642362	25.165871432017617	25.414279135194068	24.70933259814595
28-29	24.22096325268391	24.716203935520145	26.94006550699243	24.12276730480352
30-31	25.31396955389787	24.84885036044701	26.183234279591588	23.653945806063533
32-33	23.43980867109332	25.410423976691547	26.50917166561202	24.640595686603113
34-35	23.492011177052394	25.787674226329987	26.77501185031973	23.94530274629789
36-37	24.741986133740244	25.58697504471764	25.789149037040232	23.881889784501887
38-39	25.007011703466723	24.76279889657468	25.748948933744852	24.48124046621374
40-41	24.23313923255745	25.5003719083066	25.89289479377871	24.37359406535724
42-43	25.596199282637844	24.95577657746081	25.626921175692946	23.8211029642084
44-45	25.3467675174521	24.7342240610779	25.850612079788043	24.068396341681954
46-47	24.441176984760883	25.028830987347828	26.914141022047772	23.61585100584352
48-49	25.025840039379116	25.539965342767744	26.246224246428262	23.187970371424875
50-51	23.84842715493731	26.06040745125695	25.594858972314533	24.49630642149121
52-53	23.70463347221794	26.129098518338605	24.61589921704774	25.550368792395712
54-55	24.32585864498061	25.766731568594153	24.508870800461978	25.39853898596326
56-57	24.715691816214946	24.745668637607682	24.809376433856297	25.729263112321078
58-59	24.331215891736274	24.761299736807814	26.04974622477188	24.85773814668403
60-61	24.646321623294845	25.1534539789734	25.475917605845922	24.724306791885834
62-63	23.118780940597937	25.538352774920696	25.912155866232723	25.43071041824864
64-65	23.09645245779109	25.511232252357008	25.852997863596787	25.539317426255113
66-67	24.37018576657991	25.386595467360113	25.074753883310883	25.16846488274909
68-69	24.527380095673454	24.71789990848708	25.109973717840067	25.644746277999396
70-71	23.648608005718405	25.145077987720565	25.356347729016328	25.849966277544706
72-73	24.586328321859657	24.760154984057532	25.28498092399316	25.368535770089647
74-75	24.704676975785457	24.355028400800784	25.46150948803992	25.478785135373837
76-77	23.78178643794521	25.024383507362796	26.174546939207215	25.019283115484775
78-79	24.00104288857327	25.023667693243134	25.936358107033175	25.03893131115042
80-81	23.1534618693424	25.090618700942933	25.751569732069402	26.004349697645264
82-83	23.105660255351488	25.44995166512819	24.81359055789495	26.630797521625375
84-85	23.442692757671058	25.238136890553527	24.592060716824246	26.727109634951173
86-87	23.54431222061553	24.33778259882837	25.081615259694484	27.036289920861613
88-89	23.4597488840499	24.00188248928443	25.82888207492876	26.709486551736905
90-91	23.88618331204211	24.393939507863216	25.347370251988327	26.372506928106343
92-93	22.76957417507107	24.65514928425153	25.652950793776807	26.922325746900594
94-95	22.32446443923918	24.83984604267042	26.035886993109493	26.799802524980908
96-97	23.395380842228548	24.479868238174312	25.31764682883256	26.80710409076458
98-99	23.387895659888983	24.11651700874639	25.279368761250975	27.216218570113654
100	23.135737886359646	24.511961819623185	25.582689604233487	26.769610689783686
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	724.0
1	484.5
2	324.5
3	416.5
4	366.0
5	446.5
6	741.5
7	1547.5
8	2461.5
9	2539.5
10	2037.5
11	1750.5
12	1882.0
13	2107.5
14	2379.5
15	2678.0
16	3052.5
17	3506.0
18	3977.5
19	4472.5
20	5184.5
21	6132.0
22	7384.5
23	8781.5
24	10571.5
25	13177.0
26	16885.5
27	21380.5
28	26323.5
29	33325.5
30	41380.0
31	50071.0
32	59226.5
33	68826.0
34	80052.5
35	89615.5
36	99053.5
37	110815.0
38	121823.5
39	132584.5
40	142816.0
41	152094.0
42	162011.0
43	180346.0
44	203680.0
45	225412.0
46	255778.0
47	291020.0
48	365253.0
49	460535.0
50	579334.0
51	560202.5
52	418947.5
53	362771.0
54	332768.0
55	305473.0
56	281346.0
57	256307.0
58	240717.0
59	221423.5
60	188929.5
61	156022.0
62	127738.0
63	100911.5
64	76359.0
65	60537.5
66	47406.0
67	35558.0
68	27395.5
69	22171.0
70	18565.0
71	15585.5
72	13379.0
73	13183.0
74	10396.0
75	7850.0
76	6115.0
77	4231.0
78	3028.5
79	1853.5
80	1241.0
81	944.0
82	721.5
83	519.0
84	338.0
85	203.0
86	129.0
87	79.5
88	43.0
89	26.0
90	18.0
91	9.0
92	4.0
93	5.0
94	5.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.06362263020740326
3	0.02528864836685834
4	0.0069675364182226135
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.253154032054427E-4
12-13	1.629100241670755E-4
14-15	4.1354083057796096E-4
16-17	4.3860391121904946E-4
18-19	0.0
20-21	0.0029135831245265427
22-23	0.004893566495172538
24-25	7.769554998737448E-4
26-27	5.012616128217708E-4
28-29	0.005156728841903967
30-31	0.002380992660903411
32-33	0.0012343567215736106
34-35	0.001535113689266673
36-37	0.004411102192831583
38-39	0.00437350757186995
40-41	9.461312942010924E-4
42-43	0.0026754838584362017
44-45	2.882254273725182E-4
46-47	0.002124096084332254
48-49	1.2531540320544269E-5
50-51	0.0017230867940748371
52-53	0.007255761845595132
54-55	0.014536586771831351
56-57	0.025495418782147318
58-59	0.028001726846256174
60-61	0.025890162302244463
62-63	0.008320942772841395
64-65	0.0070928518214280565
66-67	1.5037848384653123E-4
68-69	0.0014724559876639518
70-71	2.3809926609034114E-4
72-73	9.586628345216366E-4
74-75	0.005319638866071043
76-77	0.001077712467566807
78-79	0.0013220775038174206
80-81	0.0
82-83	0.001491253298144768
84-85	1.2531540320544269E-5
86-87	5.764508547450364E-4
88-89	6.892347176299349E-5
90-91	6.391085563477578E-4
92-93	8.960051329189153E-4
94-95	0.0015225821489461288
96-97	1.5037848384653123E-4
98-99	0.0
100	7.393608789121119E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7979865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.950032639490324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32158617441463	33.63914682567763
2	12.037263318168938	10.34001704810991
3	3.7304352481735434	4.806669470056766
4	1.6593391849408925	2.8507468861278644
5	0.954307841145761	2.049377646266599
6	0.601345808841093	1.5496693270407393
7	0.4170068964627044	1.2537321869776001
8	0.3116944789024847	1.0709830433928516
9	0.23985003562696133	0.9271410172884816
>10	1.4799924123245902	11.963464724670258
>50	0.13114520531438517	3.903596106041629
>100	0.09640797768792657	8.429973930540037
>500	0.011874858741026525	3.457748825082229
>1k	0.006492921340325258	5.026665639965227
>5k	6.434426540449131E-4	2.0398798942936636
>10k+	6.141952606792352E-4	6.691187428468518
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	80280	1.006032056933294	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	71909	0.9011305329100179	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	35706	0.44745117868535367	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	32474	0.40694924036935465	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	28392	0.3557954927808929	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	26956	0.3378002008805913	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	26317	0.32979254661576357	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	26206	0.32840154564018315	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	26086	0.32689776080171784	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	24136	0.3024612571766565	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	22462	0.2814834586800654	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	19907	0.2494653731610748	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16748	0.20987823728847543	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	15883	0.19903845491120462	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	12421	0.15565426232148039	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	12113	0.15179454790275274	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11597	0.14532827309735188	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	11480	0.14386208287984822	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	11047	0.13843592592105256	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	10351	0.12971397385795375	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	10192	0.1277214589469872	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	9953	0.1247264208103771	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	9907	0.12414996995563207	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9724	0.1218566980769725	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	9164	0.11483903549746768	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	8993	0.11269614210265462	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	8924	0.11183146582053706	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0018922625884021849	0.0	0.0	0.0	2.506308064108854E-5
2	0.0031830112414182447	1.253154032054427E-5	0.0	0.0	2.506308064108854E-5
3	0.0033584528059058642	1.253154032054427E-5	0.0	0.0	2.506308064108854E-5
4	0.0038973090396892678	1.253154032054427E-5	0.0	2.506308064108854E-5	2.506308064108854E-5
5	0.015952650828052854	1.253154032054427E-5	0.0	8.77207822438099E-5	2.506308064108854E-5
6	0.033835158865469527	1.253154032054427E-5	0.0	1.754415644876198E-4	2.506308064108854E-5
7	0.03809588257445458	1.253154032054427E-5	0.0	2.2556772576979686E-4	2.506308064108854E-5
8	0.04365988647677624	1.253154032054427E-5	0.0	2.6316234673142965E-4	2.506308064108854E-5
9	0.047519600895503875	2.506308064108854E-5	0.0	3.6341466929578383E-4	2.506308064108854E-5
10-11	0.08307784655504824	2.506308064108854E-5	0.0	7.393608789121119E-4	2.506308064108854E-5
12-13	0.14383075402904685	2.506308064108854E-5	0.0	0.001622834471510483	3.759462096163281E-5
14-15	0.2138444697999277	4.386039112190494E-5	0.0	0.002838393882603277	4.386039112190494E-5
16-17	0.24495401864567884	5.012616128217708E-5	0.0	0.003483768209111307	8.145501208353776E-5
18-19	0.2596911100626389	5.012616128217708E-5	0.0	0.0038910432695289958	1.0025232256435416E-4
20-21	0.3117408627840195	5.012616128217708E-5	0.0	0.005037679208858796	1.3158117336571482E-4
22-23	0.32626491801553037	5.012616128217708E-5	0.0	0.006040202434502338	1.3784694352598698E-4
24-25	0.3930705594643518	5.012616128217708E-5	0.0	0.01106535010304059	1.3784694352598698E-4
26-27	0.5311931968773907	5.012616128217708E-5	0.0	0.028910263519495633	1.3784694352598698E-4
28-29	0.5640446298276975	5.012616128217708E-5	0.0	0.04671758231498904	1.3784694352598698E-4
30-31	0.575059853769456	6.265770160272135E-5	0.0	0.0888862154936205	1.3784694352598698E-4
32-33	0.5853169195218215	6.265770160272135E-5	0.0	0.1659677200052883	1.3784694352598698E-4
34-35	0.5937318488470669	7.518924192326561E-5	0.0	0.27247954695975435	1.5037848384653123E-4
36-37	0.6052170055508458	7.518924192326561E-5	0.0	0.4279583677167471	1.5037848384653123E-4
38-39	0.6167961488070287	7.518924192326561E-5	0.0	0.65965401670329	1.5037848384653123E-4
40-41	0.6275231473214147	7.518924192326561E-5	0.0	0.9635689325571297	1.5037848384653123E-4
42-43	0.6499044783339067	7.518924192326561E-5	0.0	1.2015428832442656	1.5037848384653123E-4
44-45	0.675330973644291	7.518924192326561E-5	0.0	1.4630448008832229	1.5037848384653123E-4
46-47	0.685851201743388	7.518924192326561E-5	0.0	1.7668719959548187	1.5037848384653123E-4
48-49	0.6927811435406488	7.518924192326561E-5	0.0	2.047139895223791	1.6917579432734764E-4
50-51	0.7046297149137235	7.518924192326561E-5	0.0	2.346562253872716	2.0050464512870832E-4
52-53	0.7085834158848552	7.518924192326561E-5	0.0	2.681022799257882	2.0677041528898045E-4
54-55	0.7207264784554626	7.518924192326561E-5	0.0	2.999054495282815	2.3809926609034114E-4
56-57	0.7441479273145599	7.518924192326561E-5	0.0	3.347669415460036	2.4436503625061327E-4
58-59	0.751704446127848	7.518924192326561E-5	0.0	3.705288247357568	2.506308064108854E-4
60-61	0.7576882566309079	7.518924192326561E-5	0.0	4.036509389569874	2.568965765711575E-4
62-63	0.7624063815615929	7.518924192326561E-5	0.0	4.3774863359217235	3.0702273785333464E-4
64-65	0.7658713524602234	7.518924192326561E-5	0.0	4.750023966570863	3.1328850801360677E-4
66-67	0.7708526397376396	8.145501208353776E-5	0.0	5.149103399618916	3.1328850801360677E-4
68-69	0.7763915805593202	9.398655240408204E-5	0.0	5.607782838431477	3.1328850801360677E-4
70-71	0.7838478470500441	1.0025232256435416E-4	0.0	6.127666320169577	3.1328850801360677E-4
72-73	0.8017679497084225	1.0025232256435416E-4	0.0	6.585105136490404	3.25820048334151E-4
74-75	0.8187419210725997	1.1278386288489843E-4	0.0	7.032449797083034	3.25820048334151E-4
76-77	0.8241116860999529	1.1278386288489843E-4	0.0	7.518924192326562	3.446173588149674E-4
78-79	0.8283912071194187	1.1278386288489843E-4	0.0	8.012597957484243	3.6341466929578383E-4
80-81	0.8360354467149507	1.1904963304517057E-4	0.0	8.549806293715495	3.759462096163281E-4
82-83	0.8394816203031004	1.253154032054427E-4	0.0	9.145818882900901	3.822119797766002E-4
84-85	0.8500958349546013	1.253154032054427E-4	0.0	9.713622974824762	3.8847774993687234E-4
86-87	0.8682978972701919	1.253154032054427E-4	0.0	10.290217942283483	4.1980660073823303E-4
88	0.8729095041081522	1.253154032054427E-4	0.0	10.756034093308596	4.511354515395937E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACTT	20190	0.0	18.934612	12-13
TCCGATC	1600	0.0	18.214895	3
GTATAAG	2130	0.0	17.446682	1
GAGTACT	19050	0.0	17.218513	12-13
TACTTTT	23320	0.0	16.494854	14-15
CATGGGG	16705	0.0	15.894343	4
TAGTACT	2320	0.0	15.799643	4
GTACTTT	21330	0.0	15.477963	14-15
GTACTAG	1565	0.0	15.329261	1
CTAGTAC	2450	0.0	15.157095	3
ATTGCGT	1150	0.0	15.119084	6
GTATTAG	2975	0.0	14.704876	1
TCTACAC	4020	0.0	14.61635	3
GATCTAC	2650	0.0	14.555708	1
TCTCGCG	920	0.0	14.302195	8
GTATATA	1720	0.0	14.221332	1
TATACCG	1135	0.0	14.076822	5
GTATTAC	1105	0.0	14.048082	1
ACTTTTT	23925	0.0	13.967134	16-17
TACACTC	3540	0.0	13.805486	5
>>END_MODULE