FastQCFastQC Report
Fri 27 May 2016
ERR765348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765348_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13240566
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT412040.3111951558566303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA407110.30747175007473243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195840.14790908485332122No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG163740.12366540826124804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG48950.024.2960761
TAGTACT62500.020.8288214
GTATAAG62900.020.4770891
ACGTTAA13200.019.9331388
CATGGGG324150.019.6307814
CGTTAAC16850.019.528391
CTAACGC26400.018.8705653
TCTAACG26650.018.518382
CTAGTAC70400.018.4922643
GTACATG1187250.017.4489441
TAGGCAT78600.017.338085
TACATGG1176200.017.0311182
GTACAAG56050.017.0250631
GGCTATT49550.016.973617
GTATTAG46400.016.8173831
GGGCTAT54050.016.7786756
GTGATCG44650.016.731598
CTAGGCA84500.016.6295074
CTGTGCG81250.016.595769
CTATTGA46300.016.3374219
Produced by FastQC (version 0.11.5)