##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765348_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13240566
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75314975205743	33.0	33.0	33.0	27.0	33.0
2	31.63427681263777	33.0	33.0	33.0	27.0	33.0
3	31.609585194469783	33.0	33.0	33.0	27.0	33.0
4	35.21564418016571	37.0	37.0	37.0	33.0	37.0
5	35.428014784262245	37.0	37.0	37.0	33.0	37.0
6	35.44170324742915	37.0	37.0	37.0	33.0	37.0
7	35.29055041906819	37.0	37.0	37.0	33.0	37.0
8	35.26803839050385	37.0	37.0	37.0	33.0	37.0
9	34.96818776478287	37.0	37.0	37.0	33.0	37.0
10-11	34.97938449156932	37.0	37.0	37.0	33.0	37.0
12-13	34.956999610137515	37.0	37.0	37.0	33.0	37.0
14-15	36.267080840803935	37.0	37.0	40.0	30.0	40.0
16-17	36.51239992308486	37.0	37.0	40.0	33.0	40.0
18-19	36.32813695426616	37.0	37.0	40.0	33.0	40.0
20-21	36.32187003939258	37.0	37.0	40.0	33.0	40.0
22-23	36.505038644118386	37.0	37.0	40.0	33.0	40.0
24-25	36.51900692915997	37.0	37.0	40.0	33.0	40.0
26-27	36.18569036248148	37.0	37.0	40.0	30.0	40.0
28-29	36.0697897280222	37.0	37.0	40.0	27.0	40.0
30-31	35.87744942323462	37.0	37.0	40.0	27.0	40.0
32-33	35.84100751433134	37.0	37.0	40.0	27.0	40.0
34-35	35.79098790036619	37.0	37.0	40.0	27.0	40.0
36-37	35.561615115245075	37.0	37.0	40.0	27.0	40.0
38-39	35.425668925331436	37.0	35.0	40.0	27.0	40.0
40-41	35.56856262791183	37.0	37.0	40.0	27.0	40.0
42-43	35.494331473443054	37.0	33.0	40.0	27.0	40.0
44-45	35.44183975972024	37.0	33.0	40.0	27.0	40.0
46-47	35.40920433461832	37.0	33.0	40.0	27.0	40.0
48-49	35.243690715336484	37.0	33.0	40.0	27.0	40.0
50-51	35.097809602701275	37.0	33.0	40.0	27.0	40.0
52-53	34.980602528623024	37.0	33.0	40.0	27.0	40.0
54-55	34.85246049904514	37.0	33.0	40.0	27.0	40.0
56-57	34.63883454831161	37.0	33.0	40.0	27.0	40.0
58-59	34.44015659904569	37.0	33.0	38.5	27.0	40.0
60-61	34.16120647712492	37.0	33.0	37.0	27.0	40.0
62-63	33.927915581554444	37.0	33.0	37.0	27.0	40.0
64-65	33.72216697533928	37.0	33.0	37.0	27.0	40.0
66-67	33.49973917278159	37.0	33.0	37.0	27.0	40.0
68-69	33.289962037876634	37.0	33.0	37.0	27.0	40.0
70-71	33.06937195887245	37.0	33.0	37.0	27.0	37.0
72-73	32.836914826752874	37.0	33.0	37.0	22.0	37.0
74-75	32.61484516598459	35.0	33.0	37.0	22.0	37.0
76-77	32.41877371405422	33.0	33.0	37.0	22.0	37.0
78-79	32.23372082432125	33.0	33.0	37.0	22.0	37.0
80-81	32.102492106455266	33.0	33.0	37.0	22.0	37.0
82-83	31.967185730579796	33.0	33.0	37.0	22.0	37.0
84-85	31.811494689879574	33.0	33.0	37.0	22.0	37.0
86-87	31.60749816133238	33.0	33.0	37.0	22.0	37.0
88-89	31.476443945070024	33.0	33.0	37.0	22.0	37.0
90-91	31.38624557288563	33.0	33.0	37.0	22.0	37.0
92-93	31.29602125770152	33.0	33.0	37.0	22.0	37.0
94-95	31.164931771043626	33.0	27.0	37.0	22.0	37.0
96-97	31.06505205291073	33.0	27.0	37.0	18.5	37.0
98-99	30.936924788562663	33.0	27.0	37.0	15.0	37.0
100	30.843622092892403	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	10.0
7	194.0
8	1265.0
9	2653.0
10	4546.0
11	7754.0
12	13323.0
13	22100.0
14	31277.0
15	38146.0
16	43589.0
17	51499.0
18	60781.0
19	70749.0
20	83279.0
21	97362.0
22	112691.0
23	120090.0
24	127457.0
25	142111.0
26	166383.0
27	200822.0
28	247058.0
29	309805.0
30	387378.0
31	480341.0
32	618434.0
33	818788.0
34	1085645.0
35	1479483.0
36	2222403.0
37	3092114.0
38	1099997.0
39	1039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33214246907569	18.75056577386606	12.87317461893477	25.04411713812348
2	15.729804788136276	21.061701671018145	37.411967235815204	25.79652630503037
3	17.8773342172782	26.40988249556492	29.343255453318374	26.369527833838507
4	12.645385686231316	17.406237167185342	36.656071048807526	33.29230609777581
5	14.11961716416129	37.44556394289732	33.32241313723008	15.112405755711311
6	31.703994389132177	36.347600936998006	17.484419628125895	14.463985045743923
7	27.318184404447038	31.70657915029091	22.428718460521406	18.546517984740653
8	27.182484413577075	32.60472571641955	20.283324551682632	19.929465318320737
9	27.055881642305014	14.315353903674177	19.791879169797646	38.836885284223165
10-11	25.33348111497852	25.191146243029305	26.853149731632815	22.622222910359355
12-13	26.52186442239061	22.891575728626208	26.50911554310056	24.077444305882622
14-15	24.33815732897874	23.691943116865676	24.487848396433638	27.482051157721948
16-17	23.812112520187355	26.289109393686665	25.378289664994476	24.5204884211315
18-19	24.307320971397363	25.454061587170884	27.15775813238701	23.080859309044737
20-21	25.466444875026177	24.90698445018839	26.13036958551082	23.49620108927461
22-23	24.879887017539673	24.702804264127018	25.841221450622935	24.576087267710374
24-25	24.31890290477283	25.34483851406667	25.961838241276553	24.374420339883947
26-27	24.51394370180912	25.128995030877217	25.874676964713306	24.48238430260036
28-29	24.105407570468696	25.062849732922636	26.552999673133034	24.278743023475638
30-31	24.634394158995594	25.010214353135357	26.282230463385016	24.073161024484037
32-33	24.161324475951282	25.170711222654035	25.81656430579199	24.851399995602687
34-35	24.425188041281736	25.207411362329847	26.38992323599657	23.977477360391852
36-37	24.686135119590386	25.05433453754365	25.867006491129818	24.39252385173615
38-39	24.8090088409249	24.722836734216575	25.968521847426896	24.49963257743163
40-41	24.332880991198653	24.949945081556656	26.242201310901788	24.474972616342903
42-43	24.994199175434655	24.933952213901083	25.728675570017685	24.34317304064658
44-45	24.598128685648984	25.190927060106596	25.55847036482321	24.652473889421206
46-47	24.30244064016204	25.220571812219404	26.010343499729473	24.466644047889083
48-49	25.00646537325949	25.330591803463538	25.47082352002278	24.192119303254195
50-51	24.09068992501889	25.69613604697565	25.293261612491985	24.919912415513473
52-53	24.085879094716926	25.5199087135654	25.013788165367956	25.38042402634972
54-55	24.304595437989583	25.411734664515095	25.00198631992016	25.28168357757516
56-57	24.5991636611305	24.899766369504146	24.964234912616273	25.536835056749084
58-59	24.187145776094464	24.937147701994007	25.71372704157813	25.161979480333397
60-61	24.38225450483008	25.160850374523264	25.365849163849948	25.09104595679671
62-63	23.915842206686538	25.16091744373412	25.311742734499532	25.61149761507981
64-65	23.897657017145793	24.995901789931246	25.440407947215732	25.666033245707226
66-67	24.622656293958354	25.0297703676934	25.166298289756856	25.181275048591388
68-69	24.468068353979803	24.513625891726303	25.388036818677563	25.63026893561633
70-71	23.839177092456183	24.806318673434152	25.41620583534055	25.938298398769117
72-73	24.600574093138007	24.937154160959288	25.260479476867044	25.201792269035668
74-75	24.913848969554188	24.68743097674414	25.1762359970471	25.222484056654572
76-77	24.276734024782584	24.963495656051986	25.521928001844334	25.23784231732109
78-79	24.37482933601509	25.061907292547836	25.388849546407176	25.17441382502989
80-81	23.925380489048102	24.925032429712086	25.685430867587648	25.464156213652167
82-83	24.09717542252277	24.984560662191065	25.3229648150765	25.59529910020967
84-85	24.16789170481505	25.178940914491733	24.87755660627878	25.77561077441444
86-87	23.998762091370722	24.68896223944393	25.292229328804655	26.020046340380688
88-89	24.111148610489426	24.25937122870206	25.488867461751674	26.14061269905684
90-91	24.351563525828563	24.567030835473208	25.25044166153483	25.830963977163396
92-93	24.032146124018798	24.640018108705252	25.174967358484768	26.15286840879118
94-95	23.395762788864282	24.749544397878246	25.771865031048314	26.082827782209154
96-97	24.18395679662206	24.49589173506474	25.257240019664266	26.062911448648933
98-99	23.925380586861962	24.619058153492748	25.10512640735322	26.350434852292064
100	23.721586199338574	24.74064357296734	25.22499510391742	26.312775123776667
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1493.0
1	1517.5
2	2086.5
3	2675.0
4	2277.5
5	2002.5
6	2548.0
7	4745.0
8	7823.5
9	7693.5
10	4927.5
11	3150.5
12	2648.5
13	2657.5
14	2932.5
15	3423.5
16	3936.5
17	4463.0
18	5378.0
19	6650.0
20	8628.0
21	11659.0
22	15191.0
23	18169.5
24	21574.0
25	26942.0
26	34347.0
27	41466.0
28	47912.5
29	58701.0
30	72779.0
31	88206.5
32	104526.5
33	120622.0
34	136578.0
35	149946.5
36	164624.0
37	186713.0
38	207032.5
39	224651.0
40	236736.0
41	247729.0
42	265222.5
43	297367.5
44	342429.5
45	391966.0
46	453098.0
47	511667.0
48	569943.0
49	618972.0
50	679573.0
51	693250.5
52	646959.5
53	632082.5
54	620128.5
55	584118.5
56	545112.5
57	501175.5
58	467604.5
59	431420.5
60	366048.5
61	294706.0
62	234548.0
63	183178.0
64	135743.5
65	100982.0
66	76195.5
67	55268.0
68	41794.0
69	34829.0
70	30489.0
71	26963.5
72	23428.0
73	23465.0
74	18964.5
75	13371.0
76	9858.0
77	6586.0
78	4536.0
79	2802.5
80	1733.5
81	1182.5
82	852.0
83	644.5
84	454.5
85	288.0
86	197.0
87	124.0
88	72.5
89	45.0
90	30.5
91	18.5
92	16.0
93	14.5
94	11.5
95	7.5
96	3.0
97	1.5
98	0.5
99	0.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04894050601764305
2	0.024976273672892835
3	0.022189383746888162
4	0.022793587524883755
5	0.01584524407793443
6	0.02566355547036282
7	0.04772454591442692
8	0.006329034574503839
9	0.0042671891820938775
10-11	0.0023488421869578687
12-13	0.0014085500574522267
14-15	0.00224310652580864
16-17	0.006374349857853508
18-19	0.004905379422601723
20-21	0.008405985061363691
22-23	0.01816387607599252
24-25	0.02479501253949416
26-27	0.027355326048750482
28-29	0.04398603503807919
30-31	0.04164096912473379
32-33	0.03929590321138839
34-35	0.04711278958920638
36-37	0.04448450315492555
38-39	0.05411777714034279
40-41	0.051746277311710095
42-43	0.05689711451912252
44-45	0.057153901124770645
46-47	0.061912005876485945
48-49	0.06448364820657969
50-51	0.03347288930095587
52-53	3.3608835151004873E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	6.117563252205382E-4
64-65	2.22800143135875E-4
66-67	3.8517990847219074E-4
68-69	0.0012574991129533284
70-71	1.5105094449889831E-5
72-73	2.3790523758576484E-4
74-75	7.930174586192161E-5
76-77	1.3972212366148093E-4
78-79	1.1328820837417374E-5
80-81	5.6644104187086864E-5
82-83	2.7944424732296186E-4
84-85	0.0012235126504410764
86-87	0.003727182055510316
88-89	0.0026169576134434133
90-91	0.0038253651694346
92-93	0.00396508729309608
94-95	0.002983256153853242
96-97	0.0023035269036081993
98-99	0.004176558615394539
100	0.002937940870503572
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3240566E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.235914609067304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.03309104545166	22.187835663138316
2	13.433801728550781	8.39234167336306
3	5.50962959990703	5.16294959130857
4	2.80993185635085	3.510831660890925
5	1.7148513767726505	2.6782475586056007
6	1.0671965394897267	2.000091598715594
7	0.7325991608009131	1.6018383380616186
8	0.5271474027058606	1.3172745005849502
9	0.39601017152944623	1.1132765911994291
>10	2.3183160269458085	13.625986298990325
>50	0.21827441959395016	4.75932300907993
>100	0.18767441393650633	12.144046416287127
>500	0.02959157906750912	6.489546414356146
>1k	0.02078661545882105	11.8218083301176
>5k	8.171634891607388E-4	1.624568895213123
>10k+	2.8089994939900395E-4	1.5700334600876393
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	41204	0.3111951558566303	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	40711	0.30747175007473243	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19584	0.14790908485332122	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	16374	0.12366540826124804	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5105094449889831E-5	7.5525472249449155E-6
2	0.0	0.0	0.0	1.5105094449889831E-5	7.5525472249449155E-6
3	0.0	0.0	0.0	1.5105094449889831E-5	7.5525472249449155E-6
4	0.0	2.2657641674834747E-5	0.0	1.5105094449889831E-5	7.5525472249449155E-6
5	0.0	2.2657641674834747E-5	0.0	3.0210188899779662E-5	7.5525472249449155E-6
6	0.0	2.2657641674834747E-5	0.0	3.0210188899779662E-5	7.5525472249449155E-6
7	0.0	2.2657641674834747E-5	0.0	3.776273612472458E-5	7.5525472249449155E-6
8	0.0	2.2657641674834747E-5	0.0	3.776273612472458E-5	7.5525472249449155E-6
9	0.0	3.0210188899779662E-5	0.0	4.5315283349669495E-5	1.5105094449889831E-5
10-11	0.0	3.0210188899779662E-5	0.0	6.797292502450424E-5	1.5105094449889831E-5
12-13	7.5525472249449155E-6	3.0210188899779662E-5	0.0	9.81831139242839E-5	1.888136806236229E-5
14-15	1.888136806236229E-5	3.776273612472458E-5	0.0	9.81831139242839E-5	3.776273612472458E-5
16-17	3.0210188899779662E-5	3.776273612472458E-5	0.0	1.0195938753675637E-4	4.5315283349669495E-5
18-19	4.5315283349669495E-5	4.5315283349669495E-5	0.0	1.246170292115911E-4	5.286783057461441E-5
20-21	5.286783057461441E-5	5.286783057461441E-5	0.0	3.058781626102691E-4	5.286783057461441E-5
22-23	5.286783057461441E-5	6.419665141203179E-5	0.0	0.001072461705942178	6.419665141203179E-5
24-25	5.6644104187086864E-5	8.307801947439407E-5	0.0	0.003209832570601589	7.552547224944916E-5
26-27	7.552547224944916E-5	8.307801947439407E-5	0.0	0.005687068060383521	8.307801947439407E-5
28-29	7.930174586192161E-5	8.307801947439407E-5	0.0	0.01510509444988983	8.685429308686652E-5
30-31	8.685429308686652E-5	8.307801947439407E-5	0.0	0.045874171844315415	9.063056669933899E-5
32-33	9.81831139242839E-5	8.307801947439407E-5	0.0	0.10553929492138026	9.063056669933899E-5
34-35	1.0195938753675637E-4	1.0195938753675637E-4	0.0	0.18341360935778728	9.81831139242839E-5
36-37	1.0573566114922882E-4	1.2084075559911865E-4	0.0	0.2981216966102507	9.81831139242839E-5
38-39	1.0573566114922882E-4	1.2084075559911865E-4	0.0	0.4796962607187638	9.81831139242839E-5
40-41	1.0573566114922882E-4	1.3594585004900848E-4	0.0	0.6994489510493735	9.81831139242839E-5
42-43	1.1328820837417373E-4	1.3972212366148093E-4	0.0	0.8978959056584137	9.81831139242839E-5
44-45	1.3594585004900848E-4	1.5105094449889832E-4	0.0	1.126262276099073	9.81831139242839E-5
46-47	1.4349839727395339E-4	1.5860349172384323E-4	0.0	1.3843705775115658	9.81831139242839E-5
48-49	1.4727467088642587E-4	1.5860349172384323E-4	0.0	1.6549368055716047	1.0573566114922882E-4
50-51	1.5105094449889832E-4	1.963662278485678E-4	0.0	1.9457060974583715	1.0573566114922882E-4
52-53	1.5105094449889832E-4	1.963662278485678E-4	0.0	2.2378914919498154	1.0951193476170127E-4
54-55	1.5105094449889832E-4	1.963662278485678E-4	0.0	2.530311015405233	1.1328820837417373E-4
56-57	1.5105094449889832E-4	1.963662278485678E-4	0.0	2.86247204235831	1.1328820837417373E-4
58-59	1.5105094449889832E-4	1.963662278485678E-4	0.0	3.220685580963835	1.1706448198664618E-4
60-61	1.5105094449889832E-4	2.0391877507351273E-4	0.0	3.5847863301312044	1.2084075559911865E-4
62-63	1.5105094449889832E-4	2.1147132229845764E-4	0.0	3.959249174091198	1.2084075559911865E-4
64-65	1.5105094449889832E-4	2.2657641674834745E-4	0.0	4.349081451653955	1.2084075559911865E-4
66-67	1.5105094449889832E-4	2.2657641674834745E-4	0.0	4.773995311076581	1.2084075559911865E-4
68-69	1.5105094449889832E-4	2.303526903608199E-4	0.0	5.234700691798221	1.2084075559911865E-4
70-71	1.5105094449889832E-4	2.341289639732924E-4	0.0	5.753402082660212	1.2839330282406357E-4
72-73	1.5105094449889832E-4	2.341289639732924E-4	0.0	6.253222105459843	1.5105094449889832E-4
74-75	1.5860349172384323E-4	2.341289639732924E-4	0.0	6.748706966152353	1.5105094449889832E-4
76-77	1.6615603894878814E-4	2.341289639732924E-4	0.0	7.285727060308449	1.5105094449889832E-4
78-79	1.6615603894878814E-4	2.341289639732924E-4	0.0	7.838939815714827	1.5105094449889832E-4
80-81	1.6615603894878814E-4	2.416815111982373E-4	0.0	8.435757202524425	1.5105094449889832E-4
82-83	1.6615603894878814E-4	2.4923405842318223E-4	0.0	9.069816199700224	1.5105094449889832E-4
84-85	1.6615603894878814E-4	2.4923405842318223E-4	0.0	9.697874698105807	1.5482721811137077E-4
86-87	1.6615603894878814E-4	2.5678660564812714E-4	0.0	10.333844489729518	1.5860349172384323E-4
88	1.6615603894878814E-4	2.7944424732296186E-4	0.0	10.834038363616783	1.5860349172384323E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	4895	0.0	24.296076	1
TAGTACT	6250	0.0	20.828821	4
GTATAAG	6290	0.0	20.477089	1
ACGTTAA	1320	0.0	19.933138	8
CATGGGG	32415	0.0	19.630781	4
CGTTAAC	1685	0.0	19.52839	1
CTAACGC	2640	0.0	18.870565	3
TCTAACG	2665	0.0	18.51838	2
CTAGTAC	7040	0.0	18.492264	3
GTACATG	118725	0.0	17.448944	1
TAGGCAT	7860	0.0	17.33808	5
TACATGG	117620	0.0	17.031118	2
GTACAAG	5605	0.0	17.025063	1
GGCTATT	4955	0.0	16.97361	7
GTATTAG	4640	0.0	16.817383	1
GGGCTAT	5405	0.0	16.778675	6
GTGATCG	4465	0.0	16.73159	8
CTAGGCA	8450	0.0	16.629507	4
CTGTGCG	8125	0.0	16.59576	9
CTATTGA	4630	0.0	16.337421	9
>>END_MODULE