FastQCFastQC Report
Fri 27 May 2016
ERR765347_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765347_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6349249
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA250070.39385760426154337No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT226410.3565933545841406No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG102290.16110566777267674No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG74920.11799820734704214No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC69360.10924126617179448No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG68490.10787102537638703No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC63970.1007520731979483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG26500.026.1451841
TAGGCAT36600.021.3426695
GTATAAG27900.019.1665941
CGTTAAC7800.019.076851
CTAGGCA41400.018.8681564
CTAACGC12050.018.522753
GTACAAG38250.018.4783611
TCTAACG12000.018.212432
TACATGA26200.017.9255052
GTACATA16900.017.8845461
TACGGGC18250.017.3247344
CATGGGG162400.017.2930454
TAGTACT30600.017.1702864
GTATATA19200.016.7108751
CGGGCGT19100.016.5532156
ATACGGG19400.016.5385973
TACTAGG42550.016.3924162
ATTTAGG35450.015.871591
GTCCTAT21450.015.8251141
GTACATG601750.015.6171411
Produced by FastQC (version 0.11.5)