FastQCFastQC Report
Fri 27 May 2016
ERR765347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765347_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6349249
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT254590.40097655643998215No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA240430.37867470625266075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195360.30768993309287446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127860.20137814724229589No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102630.16164116417547966No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG98980.15589245279244837No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC72320.11390323485501985No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG70990.11180849892640846No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC70780.11147775114820667No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG69400.10930426574859484No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT66670.10500454463197144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6100.030.493182
GTATCAA511450.023.6167661
GTATAAG29200.022.1488321
AGTACTT302250.022.1179512-13
GTACTAG26350.021.0128651
GGGCTAT39650.020.5244186
TAGTACT31950.020.3769874
GTGATCG19100.020.2086738
TAGGCAT36000.019.8930345
CAACGCA608400.019.7355795
ATCAACG615750.019.5377643
TAACGCA5250.019.4870534
CTAACGC10300.019.4139563
AGGGCTA39800.019.3958055
ATTTAGG39700.019.2208331
TACTTTT354400.019.17784714-15
TATCAAC631700.019.161782
CTATTGA38350.019.1596789
AACGCAG633250.019.1517476
GGCTATT39850.019.0210847
Produced by FastQC (version 0.11.5)