##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765347_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6349249 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.326394507444896 33.0 33.0 33.0 33.0 33.0 2 32.1051970083391 33.0 33.0 33.0 27.0 33.0 3 32.17021020911292 33.0 33.0 33.0 27.0 33.0 4 36.137186145952064 37.0 37.0 37.0 33.0 37.0 5 36.09324583112113 37.0 37.0 37.0 33.0 37.0 6 36.113302061393405 37.0 37.0 37.0 33.0 37.0 7 36.02865929498119 37.0 37.0 37.0 33.0 37.0 8 35.99117533427969 37.0 37.0 37.0 33.0 37.0 9 35.7276718868641 37.0 37.0 37.0 33.0 37.0 10-11 35.90193903247455 37.0 37.0 37.0 33.0 37.0 12-13 35.833781680321565 37.0 37.0 37.0 33.0 37.0 14-15 37.21671909543947 37.0 37.0 40.0 33.0 40.0 16-17 37.36257807813176 37.0 37.0 40.0 33.0 40.0 18-19 37.4423417635692 38.5 37.0 40.0 33.0 40.0 20-21 37.474826550352645 40.0 37.0 40.0 33.0 40.0 22-23 37.40522288541527 37.0 37.0 40.0 33.0 40.0 24-25 37.349818301345564 37.0 37.0 40.0 33.0 40.0 26-27 37.21782828173852 37.0 37.0 40.0 33.0 40.0 28-29 37.1616965250536 37.0 37.0 40.0 33.0 40.0 30-31 37.05450345387305 37.0 37.0 40.0 33.0 40.0 32-33 36.876250403787914 37.0 37.0 40.0 33.0 40.0 34-35 36.78469808004065 37.0 37.0 40.0 33.0 40.0 36-37 36.63757556208616 37.0 37.0 40.0 33.0 40.0 38-39 36.48657290019654 37.0 37.0 40.0 33.0 40.0 40-41 36.34704986369254 37.0 37.0 40.0 33.0 40.0 42-43 36.16626934933564 37.0 37.0 40.0 33.0 40.0 44-45 35.9414331521728 37.0 37.0 40.0 30.0 40.0 46-47 35.67064262245818 37.0 37.0 40.0 27.0 40.0 48-49 35.40131746289994 37.0 37.0 40.0 27.0 40.0 50-51 35.10008632517011 37.0 33.0 40.0 27.0 40.0 52-53 34.95553182746495 37.0 33.0 40.0 27.0 40.0 54-55 34.84249499428988 37.0 33.0 40.0 27.0 40.0 56-57 34.66669491147693 37.0 33.0 40.0 27.0 40.0 58-59 34.47088584807432 37.0 33.0 40.0 27.0 40.0 60-61 34.28899945489616 37.0 33.0 37.0 27.0 40.0 62-63 33.96858872600524 37.0 33.0 37.0 27.0 40.0 64-65 33.82259405797441 37.0 33.0 37.0 27.0 40.0 66-67 33.635885440939546 37.0 33.0 37.0 27.0 40.0 68-69 33.39722296290475 37.0 33.0 37.0 27.0 40.0 70-71 33.19165187882851 37.0 33.0 37.0 27.0 38.5 72-73 32.99192148551742 37.0 33.0 37.0 24.5 37.0 74-75 32.783686936833 37.0 33.0 37.0 22.0 37.0 76-77 32.60868875988325 37.0 33.0 37.0 22.0 37.0 78-79 32.414212610026794 33.0 33.0 37.0 22.0 37.0 80-81 32.28194421103976 33.0 33.0 37.0 22.0 37.0 82-83 32.142330533894636 33.0 33.0 37.0 22.0 37.0 84-85 31.99521337090418 33.0 33.0 37.0 22.0 37.0 86-87 31.89913271632598 33.0 33.0 37.0 22.0 37.0 88-89 31.7980621802673 33.0 33.0 37.0 22.0 37.0 90-91 31.729734099261186 33.0 33.0 37.0 22.0 37.0 92-93 31.628863586858856 33.0 33.0 37.0 22.0 37.0 94-95 31.571347414473742 33.0 33.0 37.0 22.0 37.0 96-97 31.52579179049365 33.0 33.0 37.0 22.0 37.0 98-99 30.722768629801728 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15371594455407944 1101 2 0.33394910120960475 1101 3 0.26955270213872495 1101 4 0.19191245037585247 1101 5 0.11913955541677979 1101 6 0.17013933296932038 1101 7 0.19044400298906794 1101 8 0.14512554982889014 1101 9 0.3740431580264456 1101 10-11 0.34482282797888075 1101 12-13 0.3025442753566949 1101 14-15 0.5924998155335146 1101 16-17 0.4472552585524596 1101 18-19 0.3532344942605121 1101 20-21 0.3938355416709882 1101 22-23 0.2563554375566497 1101 24-25 0.43988936323766126 1101 26-27 0.2502108681546815 1101 28-29 0.25748371886864874 1101 30-31 0.2780601035659913 1101 32-33 0.3668659503267975 1101 34-35 0.42245048571904675 1101 36-37 0.43373914465298924 1101 38-39 0.2522945342455998 1101 40-41 0.2275299644769575 1101 42-43 0.3456761521201699 1101 44-45 0.41416413882688374 1101 46-47 0.4935681467382267 1101 48-49 0.45768134043980524 1101 50-51 0.4348979735393428 1101 52-53 0.4556782358536111 1101 54-55 0.4629699399465679 1101 56-57 0.5307068659570717 1101 58-59 0.5056642249025813 1101 60-61 0.4331782828710118 1101 62-63 0.4176273539765418 1101 64-65 0.37721365728558 1101 66-67 0.3232710120667619 1101 68-69 0.2882757116081436 1101 70-71 0.2996478791847963 1101 72-73 0.35441086779374587 1101 74-75 0.24905417738222013 1101 76-77 0.24958463265791409 1101 78-79 0.5198558997967169 1101 80-81 0.4611532304877386 1101 82-83 0.3912887603523387 1101 84-85 0.44718881859089876 1101 86-87 0.2854383301871586 1101 88-89 0.36991119201612577 1101 90-91 0.22321347280830395 1101 92-93 0.3288119425082101 1101 94-95 0.42003603262881484 1101 96-97 0.32798752438381484 1101 98-99 0.39001831721159874 1102 1 0.11298867849697558 1102 2 0.33391120680895625 1102 3 0.1990425304270289 1102 4 0.19031920231211785 1102 5 0.10184965246183708 1102 6 0.19427705192494216 1102 7 0.187531939146929 1102 8 0.1566101212401989 1102 9 0.3983439351206144 1102 10-11 0.31642879742597785 1102 12-13 0.2662655119744244 1102 14-15 0.549451182947351 1102 16-17 0.39990585966209835 1102 18-19 0.30502541471032174 1102 20-21 0.3857336821262791 1102 22-23 0.25527597584704154 1102 24-25 0.3711654291255755 1102 26-27 0.24345175655826523 1102 28-29 0.22974260063855212 1102 30-31 0.2047947327036752 1102 32-33 0.32670584864204244 1102 34-35 0.36850096554934453 1102 36-37 0.3710132510810098 1102 38-39 0.28119310414199106 1102 40-41 0.2898718103831328 1102 42-43 0.24661910982561608 1102 44-45 0.3563073088790887 1102 46-47 0.43875215901289977 1102 48-49 0.4287832776723519 1102 50-51 0.3405556550321549 1102 52-53 0.4289064891936434 1102 54-55 0.3474969936569181 1102 56-57 0.5308187040728853 1102 58-59 0.39257562624010234 1102 60-61 0.2604588309155389 1102 62-63 0.3712519123967155 1102 64-65 0.32408600969486656 1102 66-67 0.34954934384993663 1102 68-69 0.27642266246842695 1102 70-71 0.3255801763925632 1102 72-73 0.4208223478981097 1102 74-75 0.33074772907591665 1102 76-77 0.30677331483481396 1102 78-79 0.47895038331286344 1102 80-81 0.43345367525060396 1102 82-83 0.39914891361318183 1102 84-85 0.4828315991249177 1102 86-87 0.3239802373804075 1102 88-89 0.3436250698692618 1102 90-91 0.33699389245210654 1102 92-93 0.316447847668627 1102 94-95 0.38010874628655245 1102 96-97 0.26794589665098556 1102 98-99 0.2919083220239287 1103 1 0.07264026358477338 1103 2 0.2845175428346991 1103 3 0.19622739849272364 1103 4 0.2281243830104458 1103 5 0.10124282441627486 1103 6 0.16397917447680044 1103 7 0.1309231343712014 1103 8 0.09053080007615222 1103 9 0.38563172183705063 1103 10-11 0.25338497900616375 1103 12-13 0.2824858644011954 1103 14-15 0.42001549182982956 1103 16-17 0.3644611251122001 1103 18-19 0.2545453980140877 1103 20-21 0.34905252881019777 1103 22-23 0.22733382980799632 1103 24-25 0.2763726857149962 1103 26-27 0.1593927390515475 1103 28-29 0.12060237012456554 1103 30-31 0.17630207055746894 1103 32-33 0.22317840718294235 1103 34-35 0.32575722514980754 1103 36-37 0.3208839580053464 1103 38-39 0.13960336648587912 1103 40-41 0.04937917418961035 1103 42-43 0.12245910930656123 1103 44-45 0.22001300749585795 1103 46-47 0.26403795494146465 1103 48-49 0.1610071856181463 1103 50-51 0.18702576969440088 1103 52-53 0.22486369212046498 1103 54-55 0.15581504283619552 1103 56-57 0.22804303645335722 1103 58-59 0.2674250979135522 1103 60-61 0.1289078060274491 1103 62-63 0.20391553466823353 1103 64-65 0.029273731475512932 1103 66-67 0.09036354473135333 1103 68-69 0.02487453563112041 1103 70-71 0.012095692048255557 1103 72-73 0.07577822020869007 1103 74-75 0.1169198311197519 1103 76-77 0.08756473682075239 1103 78-79 0.25172597523703644 1103 80-81 0.12612565645220997 1103 82-83 0.08282844326385685 1103 84-85 0.21382264598628709 1103 86-87 0.1988458646056479 1103 88-89 0.14085948054576747 1103 90-91 0.021018684844072766 1103 92-93 0.017323037380112538 1103 94-95 0.1784289852243539 1103 96-97 0.15113888358434124 1103 98-99 0.08754539306780629 1104 1 0.10310245447048061 1104 2 0.3195674217547406 1104 3 0.21443700721779635 1104 4 0.14798599361599685 1104 5 0.03186621140633861 1104 6 0.1147497830824662 1104 7 0.08445624874578073 1104 8 0.060807546591405526 1104 9 0.3616322210027789 1104 10-11 0.28322437458532335 1104 12-13 0.2112855429556788 1104 14-15 0.4281603865155432 1104 16-17 0.28920397407906506 1104 18-19 0.1722419095556802 1104 20-21 0.2649839892679893 1104 22-23 0.1685225972882094 1104 24-25 0.27102147314032266 1104 26-27 0.14420091525705203 1104 28-29 0.11685760517891453 1104 30-31 0.18070681004996914 1104 32-33 0.18160302293829034 1104 34-35 0.28584619283420665 1104 36-37 0.214832224426452 1104 38-39 -0.0038111691340105835 1104 40-41 0.043340108016806766 1104 42-43 0.10007004727553692 1104 44-45 0.11044704500429958 1104 46-47 0.20412500234391473 1104 48-49 0.3325106426635358 1104 50-51 0.2675341818202597 1104 52-53 0.3739943584314034 1104 54-55 0.21282843962668352 1104 56-57 0.26358137700701434 1104 58-59 0.21751927241285784 1104 60-61 0.2696343557272485 1104 62-63 0.2707129454611845 1104 64-65 0.15281551132405724 1104 66-67 0.22959608280618937 1104 68-69 0.16709089600295357 1104 70-71 0.20155661995861607 1104 72-73 0.2409406642273666 1104 74-75 0.20354298573955987 1104 76-77 0.16054741878225087 1104 78-79 0.2884442671865912 1104 80-81 0.2273861574898035 1104 82-83 0.15253628608260072 1104 84-85 0.24406123429397653 1104 86-87 0.22782565162109947 1104 88-89 0.1249639406560803 1104 90-91 0.17616995142331504 1104 92-93 0.17685968464314783 1104 94-95 0.2697230831275341 1104 96-97 0.16379307361802375 1104 98-99 0.09321621155974213 1105 1 0.10913725987547451 1105 2 0.2745979365442963 1105 3 0.17982286757469979 1105 4 0.14447148223195683 1105 5 -0.027991877963216893 1105 6 0.10808419820161674 1105 7 0.09646124743920836 1105 8 -0.04162411123833465 1105 9 0.2820972343635404 1105 10-11 0.2162446011702741 1105 12-13 0.2299430186576501 1105 14-15 0.4453285803266027 1105 16-17 0.2882780778124925 1105 18-19 0.1597882928787655 1105 20-21 0.23600258895947235 1105 22-23 0.13503263682255806 1105 24-25 0.28927639807562144 1105 26-27 0.10328159400038572 1105 28-29 0.1389087553841719 1105 30-31 0.22473446723907387 1105 32-33 0.21046575696227876 1105 34-35 0.2543581332207836 1105 36-37 0.12538549465092785 1105 38-39 0.1056727558097279 1105 40-41 0.0546696298112721 1105 42-43 0.1437113594114905 1105 44-45 0.2458429364660546 1105 46-47 0.3544893253286361 1105 48-49 0.3327792486771415 1105 50-51 0.2985537930517168 1105 52-53 0.2749494418413079 1105 54-55 0.31364338260112135 1105 56-57 0.3683083244322276 1105 58-59 0.2719332535813521 1105 60-61 0.19262429933389313 1105 62-63 0.2183664888014647 1105 64-65 0.170761410793844 1105 66-67 0.1659388599892182 1105 68-69 0.15215563456201409 1105 70-71 0.19125307366801536 1105 72-73 0.21646991037719232 1105 74-75 0.14521272214909686 1105 76-77 0.22080148390857346 1105 78-79 0.3583435768105474 1105 80-81 0.2692824577823032 1105 82-83 0.2148784858448849 1105 84-85 0.17946136157943116 1105 86-87 0.19845135232327138 1105 88-89 0.15415300169408752 1105 90-91 0.09317549803423475 1105 92-93 0.10830456414344525 1105 94-95 0.24257750144989387 1105 96-97 0.12528678099636537 1105 98-99 0.08537928444962972 1106 1 0.06653802777204731 1106 2 0.24953667846332195 1106 3 0.20626671538720842 1106 4 0.109304054565726 1106 5 -0.09981993020573299 1106 6 0.07719926426320711 1106 7 0.12808966120918797 1106 8 0.005011534411529794 1106 9 0.3102421041531045 1106 10-11 0.17918899253805165 1106 12-13 0.1890184873493297 1106 14-15 0.35241950674571854 1106 16-17 0.25812980431570054 1106 18-19 0.15032647248086306 1106 20-21 0.2301183286437265 1106 22-23 0.08927228482227889 1106 24-25 0.2322147784006532 1106 26-27 0.025668121014810197 1106 28-29 0.13007585738294836 1106 30-31 0.09184148076541732 1106 32-33 0.09660103070589798 1106 34-35 0.18438636882172688 1106 36-37 0.08489041858312874 1106 38-39 0.02490843359388606 1106 40-41 0.026773626173380194 1106 42-43 0.07828771229529252 1106 44-45 0.09114923352399984 1106 46-47 0.18492835432734012 1106 48-49 0.2840015245304457 1106 50-51 0.12682679252546336 1106 52-53 0.13586361765649002 1106 54-55 0.13938904962727605 1106 56-57 0.23303437290512363 1106 58-59 0.1563378576342629 1106 60-61 0.18543944829265513 1106 62-63 0.24865327076128096 1106 64-65 0.0032297488652659467 1106 66-67 0.11551458582179919 1106 68-69 0.10753474440874555 1106 70-71 0.08730274244525305 1106 72-73 0.11850313644096389 1106 74-75 0.013119059657675791 1106 76-77 0.08405932604068767 1106 78-79 0.19338793943710186 1106 80-81 0.18953667798673024 1106 82-83 0.07042854615490768 1106 84-85 0.06472703835897065 1106 86-87 0.11238075467194264 1106 88-89 -0.017291035730725213 1106 90-91 -0.019433254602873262 1106 92-93 -0.09060044973816872 1106 94-95 0.0655789461626064 1106 96-97 0.11751995323685804 1106 98-99 -0.045964037238626076 1107 1 0.0956333415201911 1107 2 0.24585273505933714 1107 3 0.17016425161399695 1107 4 0.13866677410094752 1107 5 -0.05507181370487757 1107 6 0.08531738948513379 1107 7 0.06975244441500195 1107 8 -0.009681772879091 1107 9 0.31329716213594594 1107 10-11 0.2014354041954718 1107 12-13 0.16411581529715136 1107 14-15 0.28791271493999204 1107 16-17 0.2150953010247889 1107 18-19 0.1649868673376247 1107 20-21 0.22515226538681787 1107 22-23 0.11566222745931753 1107 24-25 0.20050921976088887 1107 26-27 0.0797197674949146 1107 28-29 0.06383112399141311 1107 30-31 0.0373534814435601 1107 32-33 -0.005825011856828155 1107 34-35 0.12562762525708848 1107 36-37 0.05037044447066563 1107 38-39 0.07300356922281992 1107 40-41 0.03301369982460045 1107 42-43 0.0538855155853355 1107 44-45 -0.005759374816960872 1107 46-47 0.01813996324228384 1107 48-49 0.13668616183996107 1107 50-51 0.11548743764053171 1107 52-53 0.06989306856896604 1107 54-55 0.11538038864292588 1107 56-57 0.23959729792947826 1107 58-59 0.13856122274677318 1107 60-61 0.15948609579706385 1107 62-63 0.16531882315118906 1107 64-65 0.04735978223909143 1107 66-67 0.07905492412613313 1107 68-69 0.06020888275504177 1107 70-71 0.055669591159983156 1107 72-73 0.1003977486404608 1107 74-75 0.09195833943209664 1107 76-77 0.22467477988726614 1107 78-79 0.21076791243363147 1107 80-81 0.0961564063640239 1107 82-83 0.06289077341090632 1107 84-85 0.015894922146014068 1107 86-87 0.17075343229219797 1107 88-89 0.09578840056409632 1107 90-91 0.05489329715957325 1107 92-93 0.05543734306280612 1107 94-95 0.13005576598702362 1107 96-97 0.03925704343269132 1107 98-99 0.06951188852313805 1108 1 0.07512190924459361 1108 2 0.27155273473508856 1108 3 0.15249799526504404 1108 4 0.11622415609089387 1108 5 -0.026962998285171125 1108 6 0.05116998367853398 1108 7 0.112361252385206 1108 8 0.010101414208008919 1108 9 0.27910368434466903 1108 10-11 0.19450776815865112 1108 12-13 0.16231215353275275 1108 14-15 0.23822138081968092 1108 16-17 0.21567410470218817 1108 18-19 -0.007436652859979631 1108 20-21 0.22229609990659327 1108 22-23 0.08429714882520756 1108 24-25 0.1035211967940981 1108 26-27 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-0.08247814834430756 2215 94-95 -0.18074699108828796 2215 96-97 -0.12456635404874561 2215 98-99 0.05268791061300604 2216 1 -0.22915553517106702 2216 2 -0.009374583882809873 2216 3 -0.1486705291184265 2216 4 -0.2755039754423976 2216 5 -0.2069394387312542 2216 6 -0.32485072992265174 2216 7 -0.2584021417793636 2216 8 -0.2401864794955344 2216 9 0.10845990990294752 2216 10-11 -0.2017852344253228 2216 12-13 -0.11257124049440392 2216 14-15 -0.46395130421791464 2216 16-17 -0.608484709712684 2216 18-19 -0.6082544937041021 2216 20-21 -0.8215308117556575 2216 22-23 -0.7328226051894831 2216 24-25 -0.5967620320143183 2216 26-27 -0.6806829307863396 2216 28-29 -0.7395591878703485 2216 30-31 -0.38855991523460887 2216 32-33 -0.47560829858097975 2216 34-35 -0.4801902722300966 2216 36-37 -0.46771858924135756 2216 38-39 -0.4997961861587754 2216 40-41 -0.5275703777054019 2216 42-43 -0.5743951134221277 2216 44-45 -0.421121948085478 2216 46-47 -0.7362697932506848 2216 48-49 -0.922339930828187 2216 50-51 -0.5849251642949298 2216 52-53 -0.48304897751810216 2216 54-55 -0.48310040845259294 2216 56-57 -0.5659130729779349 2216 58-59 -0.4356342534154223 2216 60-61 -0.48067424399303604 2216 62-63 -0.5555133590333128 2216 64-65 -0.514100271648033 2216 66-67 -0.5952260767328923 2216 68-69 -0.479849209469954 2216 70-71 -0.5348581175295095 2216 72-73 -0.42142939775419563 2216 74-75 -0.49776240376468905 2216 76-77 -0.4790795214609105 2216 78-79 -0.45693696563865416 2216 80-81 -0.3428586608241666 2216 82-83 -0.3098599572564993 2216 84-85 -0.3542936676783768 2216 86-87 -0.49809945907431086 2216 88-89 -0.33407914154472707 2216 90-91 -0.3082828365667005 2216 92-93 -0.3658805455403602 2216 94-95 -0.42079653125313143 2216 96-97 -0.4744848284113772 2216 98-99 -0.4372975705350406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 12.0 9 32.0 10 91.0 11 176.0 12 301.0 13 515.0 14 1346.0 15 3639.0 16 7508.0 17 14022.0 18 22880.0 19 32777.0 20 42118.0 21 53619.0 22 63147.0 23 61511.0 24 51936.0 25 48169.0 26 54958.0 27 70429.0 28 92781.0 29 122517.0 30 162803.0 31 214339.0 32 284654.0 33 376920.0 34 500260.0 35 702128.0 36 1123175.0 37 1637649.0 38 602202.0 39 635.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69372418682541 19.11011294172449 13.016419105495745 25.179743765954356 2 15.542355336102606 21.257416460273227 37.69916625661 25.50106194701417 3 17.992348386399716 26.499968736460012 29.959055000048036 25.548627877092233 4 12.98088955087444 17.64403947616482 37.12245337991942 32.252617593041315 5 13.583767004200443 37.51461822972195 33.85119732850031 15.050417437577304 6 30.763213098116015 37.568411634194845 17.55380833229253 14.11456693539661 7 26.390695970499817 32.244648146576075 23.03029854396953 18.334357338954575 8 27.04697831192319 32.707285538809394 20.37111790701546 19.874618242251955 9 27.074618980963972 13.74031120997593 20.241201670635558 38.943868138424534 10-11 24.97105563193379 25.372481060358474 27.658302580352416 21.998160727355316 12-13 26.482061106754518 23.306598937921635 27.599468850567998 22.611871104755853 14-15 24.051158952379595 23.634673053529298 26.05605746160326 26.258110532487844 16-17 22.80730119291144 26.204092400923773 26.99783626064888 23.990770145515903 18-19 23.129018880815668 25.06955547026113 29.657350026751196 22.144075622172007 20-21 24.008863095462157 24.453451108942176 29.01090349425578 22.52678230133989 22-23 23.562022847111525 24.2558214365195 28.6291575586341 23.552998157734876 24-25 23.17675681013613 24.849033326618628 28.74520277910033 23.22900708414491 26-27 23.485690984870807 24.527451986841278 28.625708331804283 23.36114869648363 28-29 23.067688792800535 24.449852258117456 29.17445827057657 23.30800067850544 30-31 23.664751532031584 24.381600091601385 28.802973391026242 23.150674985340785 32-33 23.21376906150633 24.677808351822396 28.466303652605212 23.642118934066062 34-35 23.331302646974468 24.686927540564245 28.976568724899593 23.005201087561694 36-37 23.830038796714383 24.464594159088737 28.385892567766675 23.319474476430205 38-39 23.888683527768404 24.16977188955733 28.476186711215767 23.465357871458497 40-41 23.538177507292595 24.38667943248091 28.527539241255145 23.547603818971346 42-43 24.113552642210127 24.385498190415905 27.91599447430712 23.584954693066848 44-45 23.88465087357698 24.773761277286162 27.538939284035507 23.80264856510135 46-47 23.758494902310492 24.742642791297047 27.571851411088144 23.92701089530431 48-49 24.59390079047144 24.922207335072226 26.74699795204126 23.736893922415074 50-51 23.79875163188591 25.434338769829314 26.33616983677912 24.430739761505652 52-53 23.610639706988323 25.59953285710798 25.966521951069694 24.823305484834 54-55 23.843378957101855 25.584317137349633 25.89794478055594 24.674359124992577 56-57 24.218102094245992 25.01578746555167 25.90491768034704 24.861192759855307 58-59 23.71182009084854 24.97112650645769 26.718199270496402 24.598854132197367 60-61 23.970362479090046 25.36521248418514 26.27102039942047 24.39340463730435 62-63 23.57207127961118 25.270870617926622 26.149021718946603 25.00803638351559 64-65 23.2636962261206 25.116883902332386 26.425928483825412 25.1934913877216 66-67 24.043276614289343 24.897976122845396 26.324451915494258 24.734295347371006 68-69 23.93382821392689 24.61229794707662 26.371803681789395 25.08207015720709 70-71 23.476071354152968 24.79213624453103 26.436941733573722 25.29485066774228 72-73 24.19719245535968 24.752911722315503 26.375599696909035 24.67429612541578 74-75 24.19308960792056 24.79583018401074 26.23527601453337 24.77580419353533 76-77 23.71896266786828 24.87471352911187 26.555061866371915 24.85126193664794 78-79 23.81157693269653 25.026018976843112 26.362846287066805 24.799557803393554 80-81 23.363516985441052 25.096959256597795 26.484585626113578 25.05493813184758 82-83 23.566464317275948 24.92510531560504 26.21936074644419 25.289069620674827 84-85 23.76142438263171 25.113450425396767 25.67770613500904 25.447419056962485 86-87 23.389301632366287 24.884887960765123 26.117931427795632 25.607878979072957 88-89 23.600940835679936 24.379804603662574 26.301433445120832 25.717821115536655 90-91 23.687860368558137 24.6145161877135 26.200622154280467 25.4970012894479 92-93 23.381658178436787 24.805683691006593 26.020477592081125 25.792180538475495 94-95 22.65830574069212 24.822572610134447 26.5703550532294 25.948766595944033 96-97 23.4578195081059 24.609305140424453 26.092578906190628 25.840296445279016 98-99 23.21218619713922 24.708591520036464 26.010832147235053 26.068390135589265 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 129.0 1 135.5 2 414.0 3 718.5 4 524.5 5 435.0 6 925.0 7 2338.0 8 4109.0 9 4040.0 10 2418.0 11 1454.0 12 1449.0 13 1704.0 14 2069.0 15 2640.0 16 3286.0 17 4060.0 18 4965.0 19 6002.0 20 7371.5 21 9052.5 22 11133.5 23 13320.5 24 15923.5 25 19391.5 26 24031.5 27 28684.0 28 34588.0 29 44095.0 30 51929.5 31 61358.0 32 73935.5 33 85521.5 34 95652.5 35 106932.0 36 115538.0 37 126218.0 38 138225.0 39 134336.0 40 130785.5 41 136726.0 42 145423.5 43 160969.5 44 180466.0 45 203677.0 46 229360.0 47 253869.5 48 271786.0 49 293951.0 50 324380.5 51 308728.5 52 275540.0 53 268612.5 54 255461.0 55 242926.0 56 222860.5 57 200630.0 58 189785.0 59 168250.0 60 139407.0 61 111225.0 62 90584.5 63 72251.5 64 52544.5 65 38911.0 66 28009.5 67 20940.0 68 16379.0 69 13599.5 70 12057.5 71 10488.0 72 10130.0 73 8610.5 74 5762.0 75 4321.5 76 3244.0 77 2136.5 78 1198.0 79 784.0 80 533.5 81 355.5 82 243.0 83 149.5 84 89.5 85 53.0 86 27.5 87 14.0 88 8.5 89 7.0 90 5.5 91 1.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.053707139222292276 2 0.008394693608645684 3 0.0 4 0.0 5 0.002456983495213371 6 0.0 7 0.0 8 0.0 9 0.004535969529624684 10-11 0.0 12-13 0.0 14-15 0.0027483565379149565 16-17 0.004512344688324556 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 5.906210325032141E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.2049851880119996E-4 54-55 0.0 56-57 0.0010473679643056997 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0017009885736092567 70-71 2.8349809560154277E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 5.827460854031713E-4 80-81 9.686184933052713E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.149978840017142E-4 92-93 0.0010237431230055711 94-95 5.039966144027428E-4 96-97 2.598732543014142E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6349249.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.112650007534626 #Duplication Level Percentage of deduplicated Percentage of total 1 71.98898981787451 26.7170218350675 2 14.894444200810424 11.05544589362862 3 5.200523126781122 5.790155839809521 4 2.359514374288621 3.502713246428826 5 1.3233648529008488 2.455678830899087 6 0.8172639450686714 1.8198498454266365 7 0.5454471858593171 1.4170093354474067 8 0.3924097433959583 1.1650692372960536 9 0.29576494511658885 0.9878958803348684 >10 1.7950628191575349 12.592642915034421 >50 0.18937053665085699 4.9090445705543315 >100 0.1654428673488435 12.886438645969777 >500 0.02041686214947472 5.177778016616884 >1k 0.011342683886496402 6.987204087536973 >5k 4.2802580703529223E-4 1.0732339886839843 >10k+ 2.1401290351764611E-4 1.4628178312652387 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 25459 0.40097655643998215 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 24043 0.37867470625266075 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19536 0.30768993309287446 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12786 0.20137814724229589 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10263 0.16164116417547966 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 9898 0.15589245279244837 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 7232 0.11390323485501985 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 7099 0.11180849892640846 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 7078 0.11147775114820667 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 6940 0.10930426574859484 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 6667 0.10500454463197144 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.574989420008571E-5 0.0 2 0.0 0.0 0.0 1.574989420008571E-5 0.0 3 0.0 0.0 0.0 1.574989420008571E-5 0.0 4 0.0 0.0 0.0 1.574989420008571E-5 0.0 5 0.0 0.0 0.0 4.7249682600257135E-5 0.0 6 0.0 0.0 0.0 4.7249682600257135E-5 0.0 7 0.0 0.0 0.0 4.7249682600257135E-5 0.0 8 0.0 0.0 0.0 4.7249682600257135E-5 0.0 9 0.0 0.0 0.0 4.7249682600257135E-5 0.0 10-11 0.0 0.0 0.0 1.0237431230055713E-4 0.0 12-13 0.0 0.0 0.0 1.1024925940059998E-4 0.0 14-15 0.0 1.574989420008571E-5 0.0 1.3387410070072854E-4 0.0 16-17 2.3624841300128564E-5 1.574989420008571E-5 0.0 1.4962399490081425E-4 1.574989420008571E-5 18-19 3.149978840017142E-5 1.574989420008571E-5 0.0 1.7324883620094283E-4 1.574989420008571E-5 20-21 3.149978840017142E-5 1.574989420008571E-5 0.0 4.094972492022285E-4 3.149978840017142E-5 22-23 3.149978840017142E-5 1.574989420008571E-5 0.0 0.0014411153193078425 4.7249682600257135E-5 24-25 3.9374735500214274E-5 2.3624841300128564E-5 0.0 0.003740599872520356 4.7249682600257135E-5 26-27 8.662441810047142E-5 3.149978840017142E-5 0.0 0.00598495979603257 4.7249682600257135E-5 28-29 9.449936520051427E-5 3.149978840017142E-5 0.0 0.014285154039477742 6.299957680034284E-5 30-31 9.449936520051427E-5 3.149978840017142E-5 0.0 0.03806749428160716 6.299957680034284E-5 32-33 1.1024925940059998E-4 3.149978840017142E-5 0.0 0.07845022301062693 7.874947100042856E-5 34-35 1.1024925940059998E-4 3.149978840017142E-5 0.0 0.13282673273642284 7.874947100042856E-5 36-37 1.1024925940059998E-4 3.149978840017142E-5 0.0 0.21535617834487197 7.874947100042856E-5 38-39 1.1024925940059998E-4 3.149978840017142E-5 0.0 0.3406544616536539 7.874947100042856E-5 40-41 1.1024925940059998E-4 3.149978840017142E-5 0.0 0.48954608647416414 7.874947100042856E-5 42-43 1.1812420650064283E-4 3.149978840017142E-5 0.0 0.6263890422316089 7.874947100042856E-5 44-45 1.2599915360068568E-4 3.149978840017142E-5 0.0 0.7831477392050619 7.874947100042856E-5 46-47 1.2599915360068568E-4 3.149978840017142E-5 0.0 0.9474191357119559 7.874947100042856E-5 48-49 1.2599915360068568E-4 3.149978840017142E-5 0.0 1.1198804772028943 9.449936520051427E-5 50-51 1.2599915360068568E-4 4.7249682600257135E-5 0.0 1.3151240406542568 1.1024925940059998E-4 52-53 1.4174904780077139E-4 4.7249682600257135E-5 0.0 1.504414144097987 1.4962399490081425E-4 54-55 1.4174904780077139E-4 4.7249682600257135E-5 0.0 1.687790162269585 1.7324883620094283E-4 56-57 1.4962399490081425E-4 4.7249682600257135E-5 0.0 1.8954446423506148 1.7324883620094283E-4 58-59 1.5749894200085712E-4 4.7249682600257135E-5 0.0 2.120967377401642 1.8899873040102854E-4 60-61 1.5749894200085712E-4 4.7249682600257135E-5 0.0 2.347584730099576 1.8899873040102854E-4 62-63 1.5749894200085712E-4 4.7249682600257135E-5 0.0 2.5737768356541064 1.8899873040102854E-4 64-65 1.5749894200085712E-4 6.299957680034284E-5 0.0 2.8146557175502176 1.8899873040102854E-4 66-67 1.5749894200085712E-4 6.299957680034284E-5 0.0 3.076277210107841 1.8899873040102854E-4 68-69 1.5749894200085712E-4 6.299957680034284E-5 0.0 3.352427980065044 1.8899873040102854E-4 70-71 1.5749894200085712E-4 6.299957680034284E-5 0.0 3.670567967959675 1.8899873040102854E-4 72-73 1.6537388910089996E-4 6.299957680034284E-5 0.0 3.976226164700739 1.8899873040102854E-4 74-75 1.7324883620094283E-4 6.299957680034284E-5 0.0 4.277939012944681 1.8899873040102854E-4 76-77 1.7324883620094283E-4 6.299957680034284E-5 0.0 4.60307982881125 1.8899873040102854E-4 78-79 1.7324883620094283E-4 6.299957680034284E-5 0.0 4.93927707040628 1.8899873040102854E-4 80-81 1.7324883620094283E-4 6.299957680034284E-5 0.0 5.303753247037562 1.8899873040102854E-4 82-83 1.7324883620094283E-4 6.299957680034284E-5 0.0 5.696563483334801 1.8899873040102854E-4 84-85 1.7324883620094283E-4 6.299957680034284E-5 0.0 6.085105498303815 1.8899873040102854E-4 86-87 1.7324883620094283E-4 6.299957680034284E-5 0.0 6.481585459949672 1.8899873040102854E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 610 0.0 30.49318 2 GTATCAA 51145 0.0 23.616766 1 GTATAAG 2920 0.0 22.148832 1 AGTACTT 30225 0.0 22.11795 12-13 GTACTAG 2635 0.0 21.012865 1 GGGCTAT 3965 0.0 20.524418 6 TAGTACT 3195 0.0 20.376987 4 GTGATCG 1910 0.0 20.208673 8 TAGGCAT 3600 0.0 19.893034 5 CAACGCA 60840 0.0 19.735579 5 ATCAACG 61575 0.0 19.537764 3 TAACGCA 525 0.0 19.487053 4 CTAACGC 1030 0.0 19.413956 3 AGGGCTA 3980 0.0 19.395805 5 ATTTAGG 3970 0.0 19.220833 1 TACTTTT 35440 0.0 19.177847 14-15 TATCAAC 63170 0.0 19.16178 2 CTATTGA 3835 0.0 19.159678 9 AACGCAG 63325 0.0 19.151747 6 GGCTATT 3985 0.0 19.021084 7 >>END_MODULE