FastQCFastQC Report
Fri 27 May 2016
ERR765346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765346_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11559442
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT566330.48992849308816117No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA497040.42998615331086054No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG375840.3251368015860973No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG268520.23229494987733837No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214510.1855712412415755No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC186400.16125345842818364No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT185340.16033645914742253No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG178380.1543154072661985No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA170990.14792236511070345No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC164560.14235981286986No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC157180.13597542165097587No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA154540.13369157438568402No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC153760.1330168013300296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128310.11100016765515151No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC122990.10639786937812397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA75900.026.6230831
TATTCGC8900.025.3567399
TCTACAC120250.025.1678223
TACACTC117000.024.3086555
CTACACT159150.023.3014454
CTATTGA73350.023.0110559
GTATAAG47650.022.5841771
AGGGCTA75700.022.3562035
ATCTACA117850.022.209392
TAGTACT58750.021.680734
TTTAGGG83500.021.49752
GTATATA21300.021.4004941
GTACTAG59450.021.3424171
ATTTAGG82500.021.246461
GTACAAA40950.020.8857061
CGTTAAC19450.020.2951091
TAGGCAT81300.020.1224165
GTGATCG41550.020.0283158
TGATCGC42100.019.8783389
GCTATTG87050.019.8756458
Produced by FastQC (version 0.11.5)