##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765346_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11559442
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.760224412216438	33.0	33.0	33.0	27.0	33.0
2	31.738615497184032	33.0	33.0	33.0	27.0	33.0
3	31.722952630412436	33.0	33.0	33.0	27.0	33.0
4	35.3930856697062	37.0	37.0	37.0	33.0	37.0
5	35.48861969288829	37.0	37.0	37.0	33.0	37.0
6	35.48381807703175	37.0	37.0	37.0	33.0	37.0
7	35.41540275040958	37.0	37.0	37.0	33.0	37.0
8	35.40272238054398	37.0	37.0	37.0	33.0	37.0
9	35.38594060163112	37.0	37.0	37.0	33.0	37.0
10-11	35.363915576547726	37.0	37.0	37.0	33.0	37.0
12-13	35.34000386869885	37.0	37.0	37.0	33.0	37.0
14-15	36.85729384688292	40.0	37.0	40.0	33.0	40.0
16-17	36.90712367430885	40.0	37.0	40.0	33.0	40.0
18-19	36.959779459942794	40.0	37.0	40.0	33.0	40.0
20-21	36.972755302548336	40.0	37.0	40.0	33.0	40.0
22-23	36.96313329830281	40.0	37.0	40.0	33.0	40.0
24-25	36.93713649845728	37.0	37.0	40.0	33.0	40.0
26-27	36.92026098664624	37.0	37.0	40.0	33.0	40.0
28-29	36.88782577048269	37.0	37.0	40.0	33.0	40.0
30-31	36.78279673880452	37.0	37.0	40.0	33.0	40.0
32-33	36.65934376417131	37.0	37.0	40.0	33.0	40.0
34-35	36.54241917559688	37.0	37.0	40.0	33.0	40.0
36-37	36.466908134492996	37.0	37.0	40.0	33.0	40.0
38-39	36.35409213524321	37.0	37.0	40.0	33.0	40.0
40-41	36.1817093333744	37.0	37.0	40.0	33.0	40.0
42-43	36.05463140002779	37.0	37.0	40.0	30.0	40.0
44-45	35.873981590114816	37.0	37.0	40.0	27.0	40.0
46-47	35.62824805903261	37.0	37.0	40.0	27.0	40.0
48-49	35.36264773853271	37.0	35.0	40.0	27.0	40.0
50-51	35.12986522186797	37.0	33.0	40.0	27.0	40.0
52-53	34.944661558923	37.0	33.0	40.0	27.0	40.0
54-55	34.80954733801164	37.0	33.0	40.0	27.0	40.0
56-57	34.63334934333335	37.0	33.0	40.0	27.0	40.0
58-59	34.44428822775355	37.0	33.0	38.5	27.0	40.0
60-61	34.16323024069847	37.0	33.0	37.0	27.0	40.0
62-63	33.93682584332358	37.0	33.0	37.0	27.0	40.0
64-65	33.711338142446664	37.0	33.0	37.0	27.0	40.0
66-67	33.485049840641096	37.0	33.0	37.0	27.0	40.0
68-69	33.24300809675761	37.0	33.0	37.0	24.5	40.0
70-71	33.02442860131138	37.0	33.0	37.0	22.0	37.0
72-73	32.764607322741014	37.0	33.0	37.0	22.0	37.0
74-75	32.57710043443274	37.0	33.0	37.0	22.0	37.0
76-77	32.376446285209965	33.0	33.0	37.0	22.0	37.0
78-79	32.1758640685251	33.0	33.0	37.0	22.0	37.0
80-81	32.01006925766832	33.0	33.0	37.0	22.0	37.0
82-83	31.833003228010487	33.0	33.0	37.0	22.0	37.0
84-85	31.678480155010945	33.0	33.0	37.0	22.0	37.0
86-87	31.54253678508011	33.0	33.0	37.0	22.0	37.0
88-89	31.431933176359205	33.0	33.0	37.0	22.0	37.0
90-91	31.29976036040494	33.0	33.0	37.0	22.0	37.0
92-93	31.129825254540833	33.0	33.0	37.0	18.5	37.0
94-95	31.04377884330403	33.0	27.0	37.0	15.0	37.0
96-97	30.869231533840473	33.0	27.0	37.0	15.0	37.0
98-99	30.7438926117714	33.0	27.0	37.0	15.0	37.0
100	30.652313580534425	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	237.0
8	1445.0
9	2037.0
10	2579.0
11	6362.0
12	17041.0
13	32788.0
14	39056.0
15	38651.0
16	40565.0
17	44936.0
18	53006.0
19	63191.0
20	76535.0
21	97490.0
22	114950.0
23	108318.0
24	100439.0
25	99017.0
26	106460.0
27	125027.0
28	153341.0
29	195582.0
30	253630.0
31	336599.0
32	455413.0
33	632011.0
34	903077.0
35	1388083.0
36	2274502.0
37	2913612.0
38	882959.0
39	499.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8068290027979	19.80127863174203	12.903278002679789	24.488614362780282
2	15.72216165318239	21.507715184515057	37.49220297374472	25.277920188557836
3	18.069337254520608	27.026897485154528	29.454291440236908	25.449473820087963
4	12.799522708545947	18.068632448551533	36.518229439732785	32.61361540316974
5	13.46705961827031	37.88383552788221	33.4107435533973	15.238361300450167
6	30.493957155180386	38.03057165501458	17.127278021904722	14.348193167900309
7	25.746865197998538	33.21004503594527	22.14491529859511	18.898174467461086
8	26.52491859911312	33.23107989031482	20.04174404455236	20.2022574660197
9	26.783353344327697	14.791369162097359	19.925517227536858	38.49976026603808
10-11	24.711264158049666	26.393535858045357	26.497305489808976	22.397894494096004
12-13	26.16243054924877	24.441632466535808	26.463162961999227	22.932774022216194
14-15	24.212919535002918	24.24789935087913	24.893940125422066	26.64524098869589
16-17	22.866780949464253	27.09204350359394	25.94761937513004	24.093556171811773
18-19	23.41351691067355	26.61116698321114	28.04510395440652	21.930212151708787
20-21	24.76239844945199	25.575698218952965	26.816265528232407	22.845637803362635
22-23	24.438306410817447	25.5460375519105	26.06250727489921	23.953148762372845
24-25	23.827434710677593	26.133187116245573	26.133745119686765	23.905633053390073
26-27	24.03206760844796	25.715582880244426	26.273157234774132	23.979192276533485
28-29	23.474397623910214	25.482455618633526	27.419129702830897	23.624017054625366
30-31	24.352241531868373	25.352300244068825	26.915407660613887	23.38005056344892
32-33	23.375905034229852	26.199392485504898	26.443874433621506	23.980828046643747
34-35	23.51081726557833	26.114894487699857	27.049111895423366	23.32517635129845
36-37	23.970358164464976	25.819246178189292	26.5029963764207	23.707399280925028
38-39	24.20626842493441	25.029313354197804	26.76004922688272	24.00436899398506
40-41	23.576224869187048	25.22877774205371	27.143255021490393	24.05174236726885
42-43	24.433122063871537	25.24591630393388	26.649150178380264	23.67181145381431
44-45	23.928650927532576	25.538011459640845	26.777867741338547	23.75546987148803
46-47	23.438167695352817	25.47936930321622	26.98120609135861	24.10125691007235
48-49	24.26305174320309	25.463453907692518	26.68740795988046	23.58608638922393
50-51	23.47509508999592	25.858154553297634	25.894205869192295	24.77254448751415
52-53	22.920294883086743	26.22838028631511	25.383609932242717	25.467714898355425
54-55	23.330053309792966	25.91625733435286	25.29182842340072	25.46186093245345
56-57	23.846875004190892	25.02400219003626	25.54858301530495	25.5805397904679
58-59	23.202239168605438	24.961173731055524	26.633658984730218	25.20292811560882
60-61	23.471170706942416	25.467688060650335	25.83208374270188	25.22905748970537
62-63	22.915470849340775	25.340566755548664	25.696400460785874	26.047561934324687
64-65	22.299927624056224	25.359875764795614	25.983816010496607	26.35638060065155
66-67	23.56128785300755	25.00986594173085	25.626789053663025	25.80205715159858
68-69	23.186970913766928	24.563508386197526	25.97018743623086	26.279333263804684
70-71	22.785731335128034	24.824681786812672	25.99547843862173	26.39410843943756
72-73	23.628322125208427	24.577392298935628	26.14911092399059	25.645174651865354
74-75	23.468363469253678	24.67367628237348	26.24795896280273	25.610001285570107
76-77	22.788255525143423	24.85750007873163	26.358073002734322	25.996171393390625
78-79	22.810911472807117	24.879034188876012	26.362049231195932	25.94800510712094
80-81	22.701127908259892	24.778017180874322	26.58300521208933	25.937849698776454
82-83	22.893464813696262	24.409468198693304	26.59715922549247	26.09990776211796
84-85	23.16034471466667	24.60496145280643	25.89142088427415	26.343272948252743
86-87	22.697521980145805	24.341780842538515	26.42805631042226	26.53264086689342
88-89	22.952900599075086	23.626383180531658	26.66808053311791	26.75263568727535
90-91	22.993255958797906	23.857844350731668	26.455134821780753	26.693764868689673
92-93	22.999427220743726	23.982503631019082	26.429934632573048	26.58813451566414
94-95	22.05572521167508	24.079517325059726	26.921465314994812	26.94329214827038
96-97	22.60105426261093	24.065910575145214	26.29325726452479	27.03977789771907
98-99	22.44080063618466	24.172824111066546	26.236133205662682	27.15024204708611
100	22.53026932889686	24.04591161831793	26.191744248719367	27.232074804065842
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	743.0
1	683.5
2	630.0
3	572.5
4	445.5
5	394.5
6	486.5
7	964.0
8	1513.5
9	1475.5
10	1057.0
11	897.0
12	1039.0
13	1297.0
14	1663.0
15	2206.5
16	2908.0
17	3728.5
18	4764.0
19	6050.5
20	7582.5
21	9432.5
22	11734.0
23	14122.0
24	16960.5
25	20665.5
26	25917.5
27	31858.0
28	39433.5
29	53741.5
30	70979.5
31	86675.5
32	107575.5
33	129080.5
34	151832.0
35	172233.0
36	189400.0
37	220320.5
38	238471.5
39	242050.0
40	242552.5
41	241608.0
42	252053.0
43	279682.0
44	318161.5
45	362496.0
46	413906.0
47	468826.0
48	536571.0
49	584667.0
50	628665.0
51	644341.5
52	603213.5
53	565673.0
54	521847.5
55	465659.0
56	424347.5
57	367933.0
58	314434.0
59	285708.5
60	241675.0
61	192337.0
62	152340.0
63	121556.0
64	95025.0
65	70874.5
66	54905.0
67	42976.0
68	33710.0
69	27467.0
70	23613.0
71	20491.5
72	17296.5
73	15961.5
74	13083.5
75	10027.5
76	8079.0
77	6291.5
78	4765.5
79	3324.0
80	2307.5
81	1697.0
82	1265.0
83	943.5
84	657.0
85	455.0
86	322.5
87	166.5
88	88.0
89	60.5
90	37.0
91	21.5
92	17.0
93	13.5
94	7.5
95	6.0
96	4.0
97	3.0
98	3.5
99	3.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022120444914209527
2	0.0047407132628028235
3	0.008979672202170313
4	0.007664729837305295
5	0.00956793589171519
6	0.004887779185189043
7	0.00775989014002579
8	0.007846399506135332
9	0.0068861455423194304
10-11	0.026597304610378254
12-13	0.028734085953283903
14-15	0.01166146255156607
16-17	0.0034344218345487613
18-19	0.011228915721018367
20-21	0.0015052629703060062
22-23	3.0278278138339204E-5
24-25	0.003231124824191341
26-27	0.001526890311833391
28-29	9.905322419542398E-4
30-31	0.026562700863934433
32-33	0.020342677440658468
34-35	0.007240833943368547
36-37	0.008486568815345931
38-39	0.00620272155005406
40-41	0.014862309097619071
42-43	0.003702600869488337
44-45	0.00381506304543074
46-47	0.012933150233376317
48-49	0.017146156362910944
50-51	0.007941559808855825
52-53	0.021238049379892213
54-55	0.027012549567704044
56-57	0.01420916338349204
58-59	0.010584420943502289
60-61	0.008564427244844517
62-63	0.015537082153273488
64-65	0.00902725235353056
66-67	0.017345127904962888
68-69	0.005687990821702293
70-71	0.005216514776405297
72-73	0.010679581246222786
74-75	0.0031835446728310933
76-77	0.010294614567035329
78-79	0.0024871442756492917
80-81	0.007210555665230208
82-83	0.0017301873221908116
84-85	0.008763398786896462
86-87	0.003235450292496818
88-89	0.001064065203147349
90-91	0.0010943434812856882
92-93	1.2976404916431087E-4
94-95	0.002383333036317843
96-97	7.180277387091868E-4
98-99	0.004619600150249467
100	0.006159466866999289
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1559442E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.476831776585012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.83857500695683	21.894121655566074
2	14.23805930849649	8.678618767401757
3	5.498991589934741	5.027755248818906
4	2.5723709345355688	3.1359086495526927
5	1.478122544561929	2.252424606789585
6	0.9088423456446744	1.6619181167789803
7	0.5703801458287022	1.216836582718777
8	0.40799911045882875	0.994761620356004
9	0.30287881495276664	0.8307708022806201
>10	1.7713706821952175	10.185653437067113
>50	0.17899168814267513	3.815160911933013
>100	0.17133013379591994	11.242568851593862
>500	0.03233859777194011	6.877907235293755
>1k	0.027681592012000517	15.664209501369974
>5k	0.0015506285339282086	3.140957991675317
>10k+	5.168761779760695E-4	3.380426020803473
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	56633	0.48992849308816117	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	49704	0.42998615331086054	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	37584	0.3251368015860973	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	26852	0.23229494987733837	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21451	0.1855712412415755	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	18640	0.16125345842818364	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	18534	0.16033645914742253	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	17838	0.1543154072661985	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	17099	0.14792236511070345	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	16456	0.14235981286986	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	15718	0.13597542165097587	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	15454	0.13369157438568402	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	15376	0.1330168013300296	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12831	0.11100016765515151	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	12299	0.10639786937812397	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.488202458215543E-4	0.0	0.0	1.7301873221908115E-5	0.0
2	0.0012716876818102466	0.0	0.0	2.5952809832862175E-5	0.0
3	0.001340895174697879	0.0	0.0	2.5952809832862175E-5	0.0
4	0.0015139139069169601	0.0	0.0	3.460374644381623E-5	0.0
5	0.004870477311967135	0.0	0.0	1.903206054409893E-4	0.0
6	0.010268661757202468	0.0	0.0	2.7682997155052983E-4	4.3254683054770293E-5
7	0.011869085030228968	0.0	0.0	3.0278278138339205E-4	4.3254683054770293E-5
8	0.014767148794898577	0.0	0.0	3.2008465460530013E-4	5.190561966572435E-5
9	0.016886628264582322	0.0	0.0	3.4603746443816235E-4	6.055655627667841E-5
10-11	0.03263565836482418	0.0	0.0	5.925891578503529E-4	6.055655627667841E-5
12-13	0.06692797109064608	4.325468305477029E-6	0.0	0.0013668479845307412	8.218389780406356E-5
14-15	0.10935648969907025	8.650936610954057E-6	0.0	0.0022146397724042387	9.516030272049464E-5
16-17	0.12792139966617766	1.2976404916431086E-5	0.0	0.0025996064515916946	1.3408951746978788E-4
18-19	0.13918924460194532	1.7301873221908115E-5	0.0	0.0028461581450038853	1.47065922386219E-4
20-21	0.18541552438257833	1.7301873221908115E-5	0.0	0.0037804592989869237	1.47065922386219E-4
22-23	0.19855629709461753	1.7301873221908115E-5	0.0	0.005125679941990279	1.643677956081271E-4
24-25	0.2606786728978786	1.7301873221908115E-5	0.0	0.010735812334193986	1.8599513713551225E-4
26-27	0.424250582337798	1.7301873221908115E-5	0.0	0.024758980580550517	1.903206054409893E-4
28-29	0.4553809777323161	1.7301873221908115E-5	0.0	0.05009757391403495	1.903206054409893E-4
30-31	0.46439525368093026	1.7301873221908115E-5	0.0	0.13031770910741194	1.903206054409893E-4
32-33	0.4712943756281661	1.7301873221908115E-5	0.0	0.26860725630181803	2.2492435188480552E-4
34-35	0.4758534192221389	1.7301873221908115E-5	0.0	0.4555539964645352	2.2492435188480552E-4
36-37	0.48523968544502405	1.7301873221908115E-5	0.0	0.7558928882553327	2.2492435188480552E-4
38-39	0.49403768797836434	2.5952809832862175E-5	0.0	1.2070824871996417	2.4222622510671363E-4
40-41	0.502385841807935	2.5952809832862175E-5	0.0	1.8108054004682925	2.4222622510671363E-4
42-43	0.5226982409704551	2.5952809832862175E-5	0.0	2.2684529235926787	2.508771617176677E-4
44-45	0.5477946080788328	3.8929214749293265E-5	0.0	2.7377965130150743	2.638535666340988E-4
46-47	0.5574101241219083	4.3254683054770293E-5	0.0	3.2952628682249543	2.8980637646696094E-4
48-49	0.5651267595788794	4.758015136024732E-5	0.0	3.850397796018181	3.114337179943461E-4
50-51	0.5800193469546366	5.190561966572435E-5	0.0	4.468818650588844	3.3306105952173124E-4
52-53	0.5845697396119987	5.190561966572435E-5	0.0	5.122180638131148	3.979430841038867E-4
54-55	0.5984069127212196	5.190561966572435E-5	0.0	5.698268134396107	4.584996403805651E-4
56-57	0.6280450215503481	5.190561966572435E-5	0.0	6.323216120639733	4.7147604529699616E-4
58-59	0.6356924495144316	5.190561966572435E-5	0.0	6.988858112701288	4.758015136024732E-4
60-61	0.6405456249531768	5.190561966572435E-5	0.0	7.638543452183938	5.017543234353354E-4
62-63	0.6445985887554088	5.190561966572435E-5	0.0	8.311651202540745	5.190561966572435E-4
64-65	0.6477864588965454	5.190561966572435E-5	0.0	9.00586723822828	5.320326015736745E-4
66-67	0.6516015219419761	5.190561966572435E-5	0.0	9.698361737530238	5.536599431010597E-4
68-69	0.6551094767377179	6.055655627667841E-5	0.0	10.46988254277326	5.623108797120137E-4
70-71	0.659145138666728	6.055655627667841E-5	0.0	11.357230738300343	6.055655627667841E-4
72-73	0.6722037274809631	6.055655627667841E-5	0.0	12.166348514054572	7.223532070146639E-4
74-75	0.6853055709782532	6.055655627667841E-5	0.0	12.93862627625105	7.266786753201409E-4
76-77	0.6892157943264043	6.055655627667841E-5	0.0	13.783840085014486	7.310041436256179E-4
78-79	0.6919494902954658	6.055655627667841E-5	0.0	14.633102532111845	7.353296119310949E-4
80-81	0.6981824901236582	6.055655627667841E-5	0.0	15.567053323162138	7.526314851530031E-4
82-83	0.70028466772012	6.488202458215544E-5	0.0	16.588006583708797	8.304899146515896E-4
84-85	0.7080748361382841	6.920749288763246E-5	0.0	17.549514933333285	8.304899146515896E-4
86-87	0.7225002729370501	6.920749288763246E-5	0.0	18.490542190531343	8.391408512625437E-4
88	0.7252772235891664	6.920749288763246E-5	0.0	19.20998435737642	8.564427244844517E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACAAA	7590	0.0	26.623083	1
TATTCGC	890	0.0	25.356739	9
TCTACAC	12025	0.0	25.167822	3
TACACTC	11700	0.0	24.308655	5
CTACACT	15915	0.0	23.301445	4
CTATTGA	7335	0.0	23.011055	9
GTATAAG	4765	0.0	22.584177	1
AGGGCTA	7570	0.0	22.356203	5
ATCTACA	11785	0.0	22.20939	2
TAGTACT	5875	0.0	21.68073	4
TTTAGGG	8350	0.0	21.4975	2
GTATATA	2130	0.0	21.400494	1
GTACTAG	5945	0.0	21.342417	1
ATTTAGG	8250	0.0	21.24646	1
GTACAAA	4095	0.0	20.885706	1
CGTTAAC	1945	0.0	20.295109	1
TAGGCAT	8130	0.0	20.122416	5
GTGATCG	4155	0.0	20.028315	8
TGATCGC	4210	0.0	19.878338	9
GCTATTG	8705	0.0	19.875645	8
>>END_MODULE