FastQCFastQC Report
Fri 27 May 2016
ERR765344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14784226
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225290.15238538696581072No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT150370.10170975470748352No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA149960.10143243210703085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA472800.026.7652781
CAACGCA539850.022.1602525
ATCAACG537200.022.1130963
AACGCAG561100.021.6204266
GTGATCG41800.021.5810818
TATCAAC585800.021.5379032
GTATAAG63200.020.744361
TGATCGC44450.020.4001719
TCAACGC594600.020.0961254
GTACTAG60450.019.9778941
ACGCAGA593550.019.8684837
GTACATG778900.019.8665731
CGCAGAG592450.019.7705548
TACATGG768050.019.2598862
AATACGG29750.018.7953055
CATGGGG345900.018.5019474
GCAGAGT627750.018.0899839
CGTTAAC18250.018.023871
ACATGGG766300.017.8873543
ACGTTAA14900.017.6583468
Produced by FastQC (version 0.11.5)