##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765344_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14784226
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95217652922784	33.0	33.0	33.0	27.0	33.0
2	31.864870910387868	33.0	33.0	33.0	27.0	33.0
3	31.887782424321706	33.0	33.0	33.0	27.0	33.0
4	35.656337436941236	37.0	37.0	37.0	33.0	37.0
5	35.69788408267027	37.0	37.0	37.0	33.0	37.0
6	35.69712455694333	37.0	37.0	37.0	33.0	37.0
7	35.62693339509285	37.0	37.0	37.0	33.0	37.0
8	35.617230756618575	37.0	37.0	37.0	33.0	37.0
9	35.58914913773639	37.0	37.0	37.0	33.0	37.0
10-11	35.571300316972966	37.0	37.0	37.0	33.0	37.0
12-13	35.52372116064784	37.0	37.0	37.0	33.0	37.0
14-15	37.132674209661026	40.0	37.0	40.0	33.0	40.0
16-17	37.18068734203604	40.0	37.0	40.0	33.0	40.0
18-19	37.16322078680345	40.0	37.0	40.0	33.0	40.0
20-21	37.257563297530766	40.0	37.0	40.0	33.0	40.0
22-23	37.247347510786156	40.0	37.0	40.0	33.0	40.0
24-25	37.228099394584476	40.0	37.0	40.0	33.0	40.0
26-27	37.1873223190717	40.0	37.0	40.0	33.0	40.0
28-29	37.14820444438553	40.0	37.0	40.0	33.0	40.0
30-31	37.07061394353685	40.0	37.0	40.0	33.0	40.0
32-33	36.97133140416008	40.0	37.0	40.0	33.0	40.0
34-35	36.86305941886981	38.5	37.0	40.0	33.0	40.0
36-37	36.78342308890571	37.0	37.0	40.0	33.0	40.0
38-39	36.68915711245215	37.0	37.0	40.0	33.0	40.0
40-41	36.59891498547168	37.0	37.0	40.0	33.0	40.0
42-43	36.46708782725589	37.0	37.0	40.0	33.0	40.0
44-45	36.319812616500855	37.0	37.0	40.0	33.0	40.0
46-47	36.151024443214	37.0	37.0	40.0	30.0	40.0
48-49	35.90694802013984	37.0	37.0	40.0	27.0	40.0
50-51	35.80922961404946	37.0	37.0	40.0	27.0	40.0
52-53	35.65561328675577	37.0	37.0	40.0	27.0	40.0
54-55	35.50821459980387	37.0	35.0	40.0	27.0	40.0
56-57	35.34963849308039	37.0	33.0	40.0	27.0	40.0
58-59	35.17496448579723	37.0	33.0	40.0	27.0	40.0
60-61	34.994537454987494	37.0	33.0	40.0	27.0	40.0
62-63	34.77531735513242	37.0	33.0	38.5	27.0	40.0
64-65	34.566373850075074	37.0	33.0	37.0	27.0	40.0
66-67	34.369395800632375	37.0	33.0	37.0	27.0	40.0
68-69	34.162544593135955	37.0	33.0	37.0	27.0	40.0
70-71	33.964225384541606	37.0	33.0	37.0	27.0	40.0
72-73	33.74490774829876	37.0	33.0	37.0	27.0	38.5
74-75	33.51945793442281	37.0	33.0	37.0	27.0	37.0
76-77	33.32013275500523	37.0	33.0	37.0	27.0	37.0
78-79	33.14425675716808	37.0	33.0	37.0	27.0	37.0
80-81	32.968970780073306	37.0	33.0	37.0	27.0	37.0
82-83	32.82221020566109	37.0	33.0	37.0	27.0	37.0
84-85	32.67279717585486	37.0	33.0	37.0	24.5	37.0
86-87	32.54666236839182	33.0	33.0	37.0	22.0	37.0
88-89	32.41874725129337	33.0	33.0	37.0	22.0	37.0
90-91	32.28193636920864	33.0	33.0	37.0	22.0	37.0
92-93	32.17517112495439	33.0	33.0	37.0	22.0	37.0
94-95	32.0563886469268	33.0	33.0	37.0	22.0	37.0
96-97	31.956694756965973	33.0	33.0	37.0	22.0	37.0
98-99	31.842163769682635	33.0	33.0	37.0	22.0	37.0
100	31.77373269320964	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	3.0
6	12.0
7	192.0
8	1017.0
9	2028.0
10	3146.0
11	4986.0
12	9233.0
13	16752.0
14	28111.0
15	34965.0
16	38360.0
17	45191.0
18	54399.0
19	64581.0
20	75565.0
21	89737.0
22	105656.0
23	108045.0
24	105370.0
25	114377.0
26	131238.0
27	156859.0
28	195168.0
29	247620.0
30	321414.0
31	419666.0
32	557313.0
33	743742.0
34	989613.0
35	1361039.0
36	2264865.0
37	4202557.0
38	2287857.0
39	3548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.21617523405883	19.681920424699626	13.207635116709254	24.894269224532287
2	15.688324377203363	21.061366508297137	38.3540626124364	24.896246502063097
3	17.854140914040602	26.52313606680844	29.701841580239087	25.920881438911874
4	12.84536751085928	17.500733241376025	37.49753782416168	32.156361423603016
5	13.811270382407429	37.715931999644596	33.846687713512786	14.626109904435191
6	30.730705821190774	37.97991859702361	17.64220866212408	13.647166919661535
7	26.58438798216423	32.355998886921775	22.794504088343885	18.265109042570103
8	26.44567257021098	33.517405645719975	20.30651452433154	19.730407259737508
9	26.712477203744044	15.018926252886015	20.204148664935182	38.06444787843476
10-11	24.964624458527624	25.981255291957794	27.59620287189874	21.457917377615846
12-13	25.884263403440936	23.865716744319247	27.34866539513127	22.901354457108543
14-15	23.634950520913304	24.350168889463674	25.216666736560985	26.79821385306204
16-17	23.132305849380476	27.0821887393427	25.77191249628615	24.013592914990678
18-19	23.366662621751605	26.549905932050567	27.617374574150936	22.46605687204689
20-21	24.880670277675577	25.63856007465838	26.575144725177314	22.905624922488734
22-23	24.37343267273094	25.417757210441057	26.446709908941212	23.762100207886792
24-25	23.6881088854143	25.89818361850425	26.817415608064298	23.596291888017156
26-27	23.727900718557994	25.962626055425915	26.560873653961504	23.748599572054584
28-29	23.484505805418028	26.004859610500493	26.82713371607643	23.68350086800505
30-31	23.96555232760705	25.68971183760474	26.712800577052736	23.631935257735474
32-33	23.703381071488646	25.9591571442966	26.339425072775178	23.99803671143957
34-35	23.937829056796446	25.79319906584273	26.780497564402584	23.488474312958243
36-37	23.911745654846765	25.749855823951318	26.54806516588279	23.790333355319124
38-39	23.932757561445786	25.51339705075386	26.70254602450775	23.851299363292604
40-41	23.77135693326409	25.517618384215	26.925861773187332	23.785162909333575
42-43	23.962363847553164	25.60657264077998	26.559832998216	23.87123051345086
44-45	23.653067186739403	25.91011629622241	26.450430169820226	23.986386347217962
46-47	23.545645756498754	25.667036980147067	26.47767543484153	24.309641828512646
48-49	24.018851621865565	25.711770154575248	26.262486865610914	24.006891357948273
50-51	23.544531123480173	25.941224650711415	25.9483917822664	24.56585244354201
52-53	23.372619837402798	25.97902074363815	25.919113163487275	24.729246255471782
54-55	23.496785029561828	25.942199034705443	25.917321599374848	24.64369433635788
56-57	23.64335226348136	25.614838014187008	25.808792572834516	24.93301714949712
58-59	23.32174325886772	25.51054495629486	26.283639025860072	24.884072758977346
60-61	23.49171088023267	25.697483922157694	26.02229308810492	24.788512109504712
62-63	23.375617801895444	25.532430138618324	25.88644994819445	25.205502111291782
64-65	23.112644066936692	25.494825851295015	26.041183483988807	25.35134659777949
66-67	23.55708864799208	25.57363813129216	25.85065687008874	25.01861635062702
68-69	23.419493573537707	25.395881055934456	25.939943652676885	25.244681717850952
70-71	23.1576040395144	25.438692440927785	25.918897190110375	25.48480632944744
72-73	23.596272225970203	25.531049236194885	25.974393284935974	24.898285252898933
74-75	23.61314365558758	25.561956882226223	25.937237401548092	24.887662060638103
76-77	23.40107675052118	25.715960493758345	25.849067822538625	25.033894933181845
78-79	23.482659247901687	25.679063420605242	25.766468812039545	25.071808519453526
80-81	23.218186015011273	25.68366067450121	26.045246894634445	25.052906415853073
82-83	23.561324418273255	25.514771803797387	26.03417777895471	24.889725998974647
84-85	23.698693687385074	25.55064505964289	25.527220874675844	25.223440378296193
86-87	23.277062729470664	25.51842162500076	25.83513802880751	25.369377616721067
88-89	23.539534950417536	25.139349426253606	25.808198263291445	25.512917360037413
90-91	23.603372280413975	25.183398633689436	25.777576691153186	25.435652394743403
92-93	23.573501362886233	25.246221168114374	25.654884116453484	25.525393352545912
94-95	23.18631904891632	25.276584605378837	25.908257147577572	25.628839198127267
96-97	23.49093920940366	25.224992779370716	25.672025827869664	25.61204218335596
98-99	23.380810127354586	25.383081575676663	25.499565931576047	25.736542365392705
100	23.514771035705802	25.272189697724528	25.454286450414738	25.758752816154928
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	864.0
1	827.5
2	1141.5
3	1496.5
4	1212.0
5	1010.0
6	1335.0
7	2627.5
8	4421.5
9	4294.0
10	2699.0
11	1961.0
12	2105.5
13	2419.0
14	2875.5
15	3527.5
16	4320.0
17	5312.0
18	6490.0
19	7871.5
20	9861.0
21	12425.5
22	15570.5
23	19825.5
24	25425.0
25	32685.0
26	42511.5
27	54201.0
28	67261.5
29	85983.5
30	106650.0
31	126888.0
32	152591.0
33	177978.0
34	204044.5
35	227210.5
36	251667.5
37	288720.5
38	308228.5
39	314003.0
40	330399.5
41	346778.0
42	364943.5
43	405099.5
44	457451.5
45	505328.0
46	554342.5
47	606366.0
48	653460.0
49	675842.5
50	684682.0
51	680172.5
52	657270.0
53	642007.0
54	620831.5
55	578880.5
56	534118.0
57	481968.5
58	432661.0
59	390109.5
60	329861.0
61	262833.5
62	209972.5
63	164907.5
64	123464.5
65	95022.5
66	78014.0
67	62714.5
68	49819.5
69	40360.5
70	33462.0
71	28011.5
72	23773.5
73	22505.5
74	18180.5
75	13763.0
76	10882.0
77	8189.5
78	6262.0
79	4268.5
80	3059.5
81	2481.5
82	2079.5
83	1853.5
84	1388.0
85	832.0
86	560.5
87	316.5
88	157.0
89	105.0
90	85.0
91	71.0
92	50.5
93	30.0
94	16.5
95	11.0
96	8.5
97	5.5
98	7.0
99	7.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017444267965059514
2	0.005877886336423699
3	0.0063851837762761475
4	0.0037472370890434172
5	0.04542003078145585
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	3.61872173761413E-4
18-19	0.0015692400806102396
20-21	5.715551155670916E-4
22-23	0.0047280121394248165
24-25	0.006868807335602149
26-27	0.005401026742962398
28-29	0.021685274562225982
30-31	0.01966961273454559
32-33	0.012753457637890547
34-35	0.012976668511425623
36-37	0.013196497402028353
38-39	0.020460996740715408
40-41	0.029724247992421113
42-43	0.01750176167490946
44-45	0.01655480645385156
46-47	0.01762351306047405
48-49	0.013145767658043105
50-51	0.009902446025919788
52-53	0.026781922841276916
54-55	0.02545618553179585
56-57	0.012834625228266939
58-59	0.01572622063542589
60-61	0.01670361370287494
62-63	0.02023102190131563
64-65	0.010829109349383593
66-67	0.014836759124217934
68-69	0.007389632707183995
70-71	0.00852936095538583
72-73	0.009895682060055089
74-75	0.0032805234443791646
76-77	0.007054816396881379
78-79	0.005499104248000538
80-81	0.002289602445200716
82-83	1.995369930086296E-4
84-85	0.002438409694224101
86-87	0.0022828384793360163
88-89	0.001136346265269484
90-91	7.135983987257771E-4
92-93	0.0010822345383518894
94-95	0.003101278348964633
96-97	0.0013257373094810645
98-99	0.0018025969029423657
100	0.002211816837756674
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.4784226E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.75242685267337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.91661349972227	23.936078097886853
2	14.646298920847675	9.886962659766004
3	5.438412825933434	5.506788933058767
4	2.69903385548722	3.643957711208856
5	1.4833261760730458	2.5032929128280594
6	0.9303048216914398	1.88400272668978
7	0.6374196855136391	1.506012291682722
8	0.4533813414363763	1.224217645055858
9	0.34732150282649304	1.0550649256660616
>10	2.0277755809826097	12.928694789852502
>50	0.19966193326824222	4.700236304670078
>100	0.17600982270226626	12.345855368101741
>500	0.025635062647975954	6.014643170740043
>1k	0.018080937700474486	10.863198769532767
>5k	5.430248752178417E-4	1.1873345949716936
>10k+	1.8100829173928055E-4	0.8136590982882363
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22529	0.15238538696581072	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	15037	0.10170975470748352	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	14996	0.10143243210703085	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.0291897594097925E-5	0.0
2	0.0	0.0	0.0	2.0291897594097925E-5	0.0
3	0.0	0.0	0.0	2.0291897594097925E-5	0.0
4	0.0	1.3527931729398617E-5	0.0	2.0291897594097925E-5	0.0
5	0.0	2.0291897594097925E-5	0.0	2.0291897594097925E-5	0.0
6	1.3527931729398617E-5	2.0291897594097925E-5	0.0	2.0291897594097925E-5	0.0
7	1.3527931729398617E-5	2.0291897594097925E-5	0.0	2.0291897594097925E-5	0.0
8	1.3527931729398617E-5	2.7055863458797234E-5	0.0	2.0291897594097925E-5	0.0
9	1.3527931729398617E-5	3.381982932349654E-5	0.0	2.0291897594097925E-5	0.0
10-11	1.3527931729398617E-5	3.381982932349654E-5	0.0	2.7055863458797234E-5	0.0
12-13	3.043784639114689E-5	3.381982932349654E-5	0.0	4.734776105289516E-5	0.0
14-15	5.072974398524481E-5	4.058379518819585E-5	0.0	4.734776105289516E-5	0.0
16-17	1.0145948797048962E-4	4.058379518819585E-5	0.0	7.102164157934274E-5	0.0
18-19	1.0484147090283929E-4	4.3965778120545506E-5	0.0	8.454957330874136E-5	0.0
20-21	1.251333684969372E-4	4.734776105289516E-5	0.0	1.488072490233848E-4	0.0
22-23	1.3866130022633584E-4	4.734776105289516E-5	0.0	5.444992521082944E-4	0.0
24-25	1.555712148880841E-4	5.411172691759447E-5	0.0	0.00215432312790673	3.3819829323496542E-6
26-27	1.927730271439303E-4	6.0875692782293774E-5	0.0	0.0037573830378404656	6.7639658646993085E-6
28-29	1.9615501007627995E-4	6.0875692782293774E-5	0.0	0.015519919676552564	6.7639658646993085E-6
30-31	2.0291897594097925E-4	7.44036245116924E-5	0.0	0.06292179245636531	6.7639658646993085E-6
32-33	2.063009588733289E-4	7.44036245116924E-5	0.0	0.15434355508364117	1.3527931729398617E-5
34-35	2.2321087353507717E-4	9.469552210579032E-5	0.0	0.2726081162449762	1.3527931729398617E-5
36-37	2.2321087353507717E-4	1.1498741969988825E-4	6.7639658646993085E-6	0.46724799796756356	1.3527931729398617E-5
38-39	2.299748393997765E-4	1.2175138556458755E-4	6.7639658646993085E-6	0.7810385203797615	1.3527931729398617E-5
40-41	2.3335682233212616E-4	1.3527931729398617E-4	6.7639658646993085E-6	1.1461776896538243	1.3527931729398617E-5
42-43	2.435027711291751E-4	1.4204328315868547E-4	6.7639658646993085E-6	1.4788701146749244	1.3527931729398617E-5
44-45	2.4688475406152476E-4	1.5218923195573446E-4	6.7639658646993085E-6	1.8580343671694413	1.3527931729398617E-5
46-47	2.502667369938744E-4	1.555712148880841E-4	6.7639658646993085E-6	2.2828046595066933	1.3527931729398617E-5
48-49	2.502667369938744E-4	1.555712148880841E-4	6.7639658646993085E-6	2.7366464771304226	2.0291897594097925E-5
50-51	2.7055863458797235E-4	1.9615501007627995E-4	6.7639658646993085E-6	3.2078547771117676	2.0291897594097925E-5
52-53	2.7055863458797235E-4	1.9615501007627995E-4	1.0145948797048962E-5	3.652737721947703	2.367388052644758E-5
54-55	2.7055863458797235E-4	1.9615501007627995E-4	2.0291897594097925E-5	4.102500868155019	2.7055863458797234E-5
56-57	2.7055863458797235E-4	2.0291897594097925E-4	2.0291897594097925E-5	4.609395852038517	2.7055863458797234E-5
58-59	2.7055863458797235E-4	2.0291897594097925E-4	2.0291897594097925E-5	5.166780459119064	2.7055863458797234E-5
60-61	2.7055863458797235E-4	2.0291897594097925E-4	2.0291897594097925E-5	5.744135675415135	2.7055863458797234E-5
62-63	2.773226004526717E-4	2.1306492473802824E-4	2.0291897594097925E-5	6.3213995781720325	2.7055863458797234E-5
64-65	2.773226004526717E-4	2.435027711291751E-4	2.0291897594097925E-5	6.891216354511897	2.7055863458797234E-5
66-67	2.9423251511441993E-4	2.435027711291751E-4	2.0291897594097925E-5	7.500433232013634	2.7055863458797234E-5
68-69	3.0099648097911926E-4	2.4688475406152476E-4	2.0291897594097925E-5	8.171685822443461	2.7055863458797234E-5
70-71	3.0776044684381853E-4	2.502667369938744E-4	2.7055863458797234E-5	8.902549244038884	3.381982932349654E-5
72-73	3.2805234443791646E-4	2.5703070285857375E-4	2.7055863458797234E-5	9.623993843167712	3.381982932349654E-5
74-75	3.314343273702661E-4	2.671766516556227E-4	2.7055863458797234E-5	10.324321340866948	3.381982932349654E-5
76-77	3.3819829323496545E-4	2.773226004526716E-4	2.7055863458797234E-5	11.056909573757867	3.381982932349654E-5
78-79	3.3819829323496545E-4	2.8408656631737095E-4	2.7055863458797234E-5	11.819458793446476	3.381982932349654E-5
80-81	3.449622590996647E-4	2.8408656631737095E-4	2.7055863458797234E-5	12.636403826618992	3.72018122558462E-5
82-83	3.449622590996647E-4	2.874685492497206E-4	2.7055863458797234E-5	13.48517331918492	4.058379518819585E-5
84-85	3.449622590996647E-4	2.9085053218207027E-4	3.381982932349654E-5	14.299470259721408	4.058379518819585E-5
86-87	3.5172622496436405E-4	2.9423251511441993E-4	3.381982932349654E-5	15.115427753877679	4.058379518819585E-5
88	3.5172622496436405E-4	3.179063956408675E-4	3.381982932349654E-5	15.738389010016487	4.058379518819585E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	47280	0.0	26.765278	1
CAACGCA	53985	0.0	22.160252	5
ATCAACG	53720	0.0	22.113096	3
AACGCAG	56110	0.0	21.620426	6
GTGATCG	4180	0.0	21.581081	8
TATCAAC	58580	0.0	21.537903	2
GTATAAG	6320	0.0	20.74436	1
TGATCGC	4445	0.0	20.400171	9
TCAACGC	59460	0.0	20.096125	4
GTACTAG	6045	0.0	19.977894	1
ACGCAGA	59355	0.0	19.868483	7
GTACATG	77890	0.0	19.866573	1
CGCAGAG	59245	0.0	19.770554	8
TACATGG	76805	0.0	19.259886	2
AATACGG	2975	0.0	18.795305	5
CATGGGG	34590	0.0	18.501947	4
GCAGAGT	62775	0.0	18.089983	9
CGTTAAC	1825	0.0	18.02387	1
ACATGGG	76630	0.0	17.887354	3
ACGTTAA	1490	0.0	17.658346	8
>>END_MODULE