FastQCFastQC Report
Fri 27 May 2016
ERR765342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765342_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12390110
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA756430.6105111254056663No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT738830.5963062474828714No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG311130.2511115720522255No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT227340.1834850538050106No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC223330.18024860150555566No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC216140.17444558603595933No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG204100.1647281581842292No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG190920.1540906416488635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172760.13943379033761605No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC142260.11481738257368174No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA140390.1133081142943848No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC130890.10564070859742165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG42950.023.623761
TAGTACT51150.020.3862674
TACATGA45250.020.242572
GTATAAG47500.020.1741371
GTACATA31800.018.7600941
CTAGTAC58250.018.5469633
CATGGGG306200.018.4386734
GTACAAG67650.018.0536061
TAGGCAT68300.016.9850985
GTGATCG34850.016.4411838
GTATATA29250.016.3807091
GTATTAG47150.016.3388161
CTAGGCA75000.016.0954174
TGATCGC35650.016.0722379
CTATTGA52250.015.8198529
ATTTAGG54700.015.8011571
TACTAGG65150.015.6456992
GTACAAA50650.015.5807461
CTGTGCG68750.015.3704249
ATAAGGT44800.015.2030533
Produced by FastQC (version 0.11.5)