##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765342_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12390110
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.731019498616234	33.0	33.0	33.0	27.0	33.0
2	31.588953286129016	33.0	33.0	33.0	27.0	33.0
3	31.623259922631842	33.0	33.0	33.0	27.0	33.0
4	35.3453008084674	37.0	37.0	37.0	33.0	37.0
5	35.419384977211664	37.0	37.0	37.0	33.0	37.0
6	35.43090319617824	37.0	37.0	37.0	33.0	37.0
7	35.242867416027785	37.0	37.0	37.0	33.0	37.0
8	35.212856383034534	37.0	37.0	37.0	33.0	37.0
9	35.073321705779854	37.0	37.0	37.0	33.0	37.0
10-11	35.05808939549367	37.0	37.0	37.0	33.0	37.0
12-13	35.01094251786304	37.0	37.0	37.0	33.0	37.0
14-15	36.56023368638374	37.0	37.0	40.0	33.0	40.0
16-17	36.600827393784236	37.0	37.0	40.0	33.0	40.0
18-19	36.551127754313725	38.5	37.0	40.0	33.0	40.0
20-21	36.27333361043607	37.0	37.0	40.0	27.0	40.0
22-23	36.332084783750915	37.0	37.0	40.0	33.0	40.0
24-25	36.237605073724126	37.0	37.0	40.0	27.0	40.0
26-27	36.206474518789584	37.0	37.0	40.0	27.0	40.0
28-29	36.1370940613118	37.0	37.0	40.0	27.0	40.0
30-31	36.23454368040316	37.0	37.0	40.0	33.0	40.0
32-33	35.99402063419937	37.0	37.0	40.0	27.0	40.0
34-35	35.81976975991335	37.0	37.0	40.0	27.0	40.0
36-37	35.91839063575706	37.0	37.0	40.0	27.0	40.0
38-39	35.834834476852905	37.0	37.0	40.0	27.0	40.0
40-41	35.852270520600705	37.0	37.0	40.0	27.0	40.0
42-43	35.736229944689754	37.0	37.0	40.0	27.0	40.0
44-45	35.63889727371266	37.0	37.0	40.0	27.0	40.0
46-47	35.42636433413424	37.0	33.0	40.0	27.0	40.0
48-49	35.182419728315566	37.0	33.0	40.0	27.0	40.0
50-51	34.99040113445321	37.0	33.0	40.0	27.0	40.0
52-53	34.849933939246704	37.0	33.0	40.0	27.0	40.0
54-55	34.71534715995257	37.0	33.0	40.0	27.0	40.0
56-57	34.55005867583097	37.0	33.0	40.0	27.0	40.0
58-59	34.36826848187789	37.0	33.0	40.0	27.0	40.0
60-61	34.12382767384632	37.0	33.0	37.0	27.0	40.0
62-63	33.87050219893125	37.0	33.0	37.0	27.0	40.0
64-65	33.68166412566151	37.0	33.0	37.0	27.0	40.0
66-67	33.40831675424997	37.0	33.0	37.0	22.0	40.0
68-69	33.20322975340816	37.0	33.0	37.0	22.0	40.0
70-71	32.88939496905193	37.0	33.0	37.0	22.0	37.0
72-73	32.65608969573313	37.0	33.0	37.0	22.0	37.0
74-75	32.45193541461698	37.0	33.0	37.0	22.0	37.0
76-77	32.17299313726835	33.0	33.0	37.0	22.0	37.0
78-79	32.08664571985236	33.0	33.0	37.0	22.0	37.0
80-81	31.933754220099743	33.0	33.0	37.0	22.0	37.0
82-83	31.787953698554734	33.0	33.0	37.0	22.0	37.0
84-85	31.62033303174871	33.0	33.0	37.0	22.0	37.0
86-87	31.442708983213222	33.0	33.0	37.0	22.0	37.0
88-89	31.294610176987938	33.0	33.0	37.0	22.0	37.0
90-91	31.165966202075687	33.0	33.0	37.0	15.0	37.0
92-93	31.024933757650253	33.0	27.0	37.0	15.0	37.0
94-95	30.887878517624138	33.0	27.0	37.0	15.0	37.0
96-97	30.758887330298116	33.0	27.0	37.0	15.0	37.0
98-99	30.63391249149523	33.0	27.0	37.0	15.0	37.0
100	30.40573126469418	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	3.0
6	23.0
7	343.0
8	1635.0
9	3233.0
10	4972.0
11	9073.0
12	17618.0
13	32343.0
14	45401.0
15	50900.0
16	56770.0
17	62229.0
18	69629.0
19	78386.0
20	88650.0
21	101405.0
22	115467.0
23	117360.0
24	117880.0
25	126648.0
26	143786.0
27	170301.0
28	207572.0
29	257485.0
30	321711.0
31	405188.0
32	527779.0
33	697532.0
34	935864.0
35	1320090.0
36	2104113.0
37	3068820.0
38	1128943.0
39	957.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.8591047330364	18.349885069533435	12.665946879723602	25.12506331770656
2	15.669433046444622	21.060132499208798	37.16543158777328	26.1050028665733
3	18.277487468410207	26.553853771092186	28.898228259494736	26.270430501002874
4	13.013245177441016	17.600296134575277	36.051131644442854	33.33532704354086
5	13.964108833362717	37.524540527065945	33.289531721537344	15.221818918033994
6	31.71356384290305	36.26042535757545	17.5892196235511	14.436791175970406
7	27.2985272676003	31.825764029731545	22.362369943862426	18.51333875880573
8	27.35354457636226	32.699965316316884	20.159646644541322	19.786843462779533
9	27.5182145771662	14.564502698520146	19.318222632088826	38.59906009222483
10-11	25.228637980787255	25.43495261704416	26.643183588388	22.69322581378059
12-13	26.65928140646067	23.33655192502305	26.379721153225695	23.624445515290585
14-15	24.713192578436885	23.784639641193863	24.30907158628819	27.19309619408106
16-17	23.78540691683067	26.296393238650158	25.717355482894526	24.200844361624647
18-19	24.421320052201395	25.432413373455887	27.354742932984617	22.791523641358104
20-21	25.134503094846476	24.85649317241821	26.639498612755226	23.369505119980087
22-23	24.402531984083744	24.86442531042886	25.882622356851027	24.85042034863637
24-25	24.09505874177619	25.466276042391954	25.984086508891206	24.45457870694065
26-27	24.53245115821813	24.940308469313017	25.965782547278625	24.561457825190228
28-29	24.03242122238065	24.8395285854864	26.865750027783196	24.26230016434976
30-31	24.74112035782985	24.960771530243363	26.321203154425138	23.97690495750165
32-33	23.74488078002363	25.202265907811572	26.161406127529897	24.891447184634895
34-35	23.94808145075374	25.39126283033795	26.70494780820628	23.955707910702024
36-37	24.70547811315115	25.15326522944911	25.943227007914395	24.198029649485346
38-39	24.88961306211989	24.69153307578871	25.91817797096361	24.50067589112779
40-41	24.178168242437085	25.230854769946216	26.241824799834085	24.349152187782614
42-43	25.086316287911288	24.964804272429646	25.74700960972865	24.201869829930416
44-45	24.71340216407628	25.149699921904116	25.52325128851745	24.61364662550215
46-47	24.13326041944863	25.161447126633774	26.431210533997312	24.274081919920288
48-49	24.996207844748902	25.363387793810272	25.62668469297996	24.01371966846086
50-51	23.853685465409065	25.82960666370237	25.39325898041484	24.923448890473722
52-53	23.698904338583457	25.80472266947147	24.814168694678767	25.68220429726631
54-55	24.09146762363408	25.653202920957348	24.83349412861739	25.421835326791186
56-57	24.541778729859708	24.807396348341	24.782625121938853	25.868199799860434
58-59	24.032038277697364	24.78715417396913	25.784581392176065	25.396226156157443
60-61	24.28265112861845	25.200380421484464	25.31260007719188	25.204368372705204
62-63	23.427024500029727	25.22717701900045	25.354000341397725	25.991798139572094
64-65	23.29874086203128	25.04360370949913	25.653298791318775	26.004356637150817
66-67	24.3605291221815	24.796740107598765	25.285495496306453	25.557235273913285
68-69	24.424228063596427	24.37692673784336	25.245837317831565	25.953007880728645
70-71	23.49579526504314	24.680632449231872	25.534105162131194	26.28946712359379
72-73	24.385902217845494	24.519831850677924	25.46173915089848	25.632526780578104
74-75	24.580755445049	24.26784070910764	25.444766456237787	25.706637389605568
76-77	23.846757218517244	24.596826506046078	26.00166596847658	25.5547503069601
78-79	24.0863364342499	24.644437638824456	25.758968917086598	25.510257009839044
80-81	23.392917308318665	24.690280428916786	25.981568618285756	25.9352336444788
82-83	23.536484333133064	24.770677679326415	25.30702654473172	26.385811442808805
84-85	23.67855242751602	24.87592570316303	24.952323209163502	26.493198660157447
86-87	23.669550930775642	24.245059511915503	25.34350192856148	26.741887628747374
88-89	23.730218554178435	23.84326374928736	25.70389546137105	26.72262223516315
90-91	24.06092946732311	24.14069147064882	25.40020773886407	26.398171323164
92-93	23.43184230652085	24.32930032894186	25.43892251912503	26.799934845412267
94-95	22.795911926658242	24.420452735335527	25.97827804333815	26.80535729466808
96-97	23.852085762429297	24.068085991399958	25.385753188927072	26.694075057243673
98-99	23.69993476779592	24.035655158162157	25.298259003782047	26.966151070259876
100	23.33783702274935	24.24694547553623	25.400243091030106	27.01497441068431
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	732.0
1	720.5
2	945.5
3	1239.0
4	1089.5
5	971.5
6	1269.0
7	2307.5
8	3863.5
9	3984.0
10	2702.0
11	1932.5
12	1908.5
13	2069.5
14	2305.5
15	2785.5
16	3350.0
17	3916.5
18	4770.0
19	5690.5
20	6953.0
21	8763.0
22	11247.5
23	14148.5
24	17447.0
25	21562.5
26	27659.0
27	34989.0
28	42624.0
29	54699.5
30	69503.0
31	83642.5
32	100151.0
33	116439.0
34	132700.0
35	147929.0
36	162722.0
37	184416.0
38	200140.5
39	209573.5
40	217757.5
41	224853.5
42	241173.0
43	275966.0
44	318421.0
45	361517.5
46	418347.0
47	475471.0
48	542003.0
49	612815.5
50	721042.0
51	735214.5
52	626977.0
53	576308.5
54	551108.0
55	517686.5
56	486759.5
57	444801.0
58	410191.5
59	377257.5
60	324186.5
61	265542.5
62	216663.0
63	171435.5
64	128016.5
65	98437.0
66	74755.5
67	53590.5
68	40239.0
69	32482.5
70	28050.5
71	24632.0
72	21131.5
73	21386.5
74	17518.5
75	12731.5
76	9349.5
77	6153.0
78	4315.5
79	2671.0
80	1664.5
81	1156.5
82	838.5
83	607.5
84	436.5
85	283.5
86	203.0
87	132.0
88	89.5
89	67.5
90	45.5
91	37.0
92	28.0
93	17.5
94	14.0
95	11.0
96	7.0
97	3.5
98	1.5
99	2.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017635033103015226
2	0.0060774278840139435
3	0.009838492152208497
4	0.009265454463277565
5	0.019676984304416993
6	0.003930554288864263
7	0.023107139484637343
8	0.008062882411859136
9	0.0013801330254533657
10-11	4.317960050395033E-4
12-13	9.321951136834136E-4
14-15	0.0010694013208922277
16-17	0.001275210631705449
18-19	0.0032525942061854174
20-21	0.0064890465056403855
22-23	0.013264611855746237
24-25	0.02226776033465401
26-27	0.026654323488653448
28-29	0.03248962277171066
30-31	0.034987582838247606
32-33	0.035786607221404816
34-35	0.04516505503179552
36-37	0.04202545417272325
38-39	0.04615778229571812
40-41	0.04294957833304143
42-43	0.0496323277194472
44-45	0.04305046525010674
46-47	0.04549999959645233
48-49	0.048357117087741756
50-51	0.04893822573003791
52-53	0.03399889105100762
54-55	0.030346784653243596
56-57	0.022227405567827888
58-59	0.02211441222071475
60-61	0.022574456562532534
62-63	0.022586562992580373
64-65	0.026331485354044476
66-67	0.027792327913150085
68-69	0.028772948747024844
70-71	0.02903929020807725
72-73	0.027291928804506177
74-75	0.02187228361975802
76-77	0.0268036361259101
78-79	0.02654133014154031
80-81	0.025330687136756656
82-83	0.02740895762830193
84-85	0.02889401304750321
86-87	0.03175920149215786
88-89	0.03158567599480553
90-91	0.03136776025394448
92-93	0.03375676245005089
94-95	0.031743059585427405
96-97	0.034249090605329574
98-99	0.035161108335599926
100	0.04397862488710754
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.239011E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.17531899149809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57856643108487	24.462203407364623
2	13.657888679489751	9.335254047438656
3	5.389636956136013	5.5257768667294505
4	2.7244974497199914	3.724422777428149
5	1.5577345722195446	2.661803795484387
6	0.9968241520839249	2.0440070025538644
7	0.7086915033952851	1.6953830736568754
8	0.48916595089333975	1.33739219292475
9	0.3808348926621424	1.1713638545839484
>10	2.1514939922297613	13.65747586170073
>50	0.18152877130008668	4.349397453925399
>100	0.14421667538065736	9.99523078444744
>500	0.02282820839978929	5.456348125200074
>1k	0.014889789394137239	9.550456098428711
>5k	8.340238931956969E-4	1.8492935213502981
>10k+	3.679517175863369E-4	3.1841911367826916
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	75643	0.6105111254056663	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	73883	0.5963062474828714	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	31113	0.2511115720522255	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	22734	0.1834850538050106	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	22333	0.18024860150555566	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	21614	0.17444558603595933	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	20410	0.1647281581842292	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	19092	0.1540906416488635	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17276	0.13943379033761605	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	14226	0.11481738257368174	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	14039	0.1133081142943848	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	13089	0.10564070859742165	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	8.07095336522436E-6	0.0	0.0	0.0	0.0
5	8.07095336522436E-6	0.0	0.0	1.614190673044872E-5	0.0
6	8.07095336522436E-6	0.0	0.0	1.614190673044872E-5	0.0
7	8.07095336522436E-6	0.0	0.0	1.614190673044872E-5	0.0
8	8.07095336522436E-6	0.0	0.0	1.614190673044872E-5	0.0
9	8.07095336522436E-6	0.0	0.0	1.614190673044872E-5	0.0
10-11	8.07095336522436E-6	0.0	0.0	5.649667355657052E-5	0.0
12-13	8.07095336522436E-6	0.0	0.0	5.649667355657052E-5	4.03547668261218E-6
14-15	8.07095336522436E-6	0.0	0.0	9.685144038269232E-5	8.07095336522436E-6
16-17	2.01773834130609E-5	0.0	0.0	1.654545439870994E-4	8.07095336522436E-6
18-19	2.824833677828526E-5	0.0	0.0	2.259866942262821E-4	8.07095336522436E-6
20-21	4.03547668261218E-5	8.07095336522436E-6	0.0	4.317960050395033E-4	1.210643004783654E-5
22-23	4.439024350873398E-5	8.07095336522436E-6	0.0	0.0016706873466014426	1.614190673044872E-5
24-25	4.842572019134616E-5	8.07095336522436E-6	0.0	0.004588336988130048	2.824833677828526E-5
26-27	7.263858028701925E-5	8.07095336522436E-6	0.0	0.007582660686628287	3.228381346089744E-5
28-29	7.263858028701925E-5	8.07095336522436E-6	0.0	0.020088602926043433	3.228381346089744E-5
30-31	7.263858028701925E-5	8.07095336522436E-6	8.07095336522436E-6	0.057622571551019325	3.228381346089744E-5
32-33	7.263858028701925E-5	8.07095336522436E-6	8.07095336522436E-6	0.12685117404123128	3.228381346089744E-5
34-35	7.263858028701925E-5	8.07095336522436E-6	8.07095336522436E-6	0.21654771426565222	3.228381346089744E-5
36-37	7.263858028701925E-5	8.07095336522436E-6	8.07095336522436E-6	0.3525109946562218	3.228381346089744E-5
38-39	8.07095336522436E-5	8.07095336522436E-6	8.07095336522436E-6	0.5677270016166119	3.228381346089744E-5
40-41	8.07095336522436E-5	8.07095336522436E-6	8.07095336522436E-6	0.8453597264269648	3.228381346089744E-5
42-43	8.878048701746797E-5	8.07095336522436E-6	8.07095336522436E-6	1.0845343584520233	3.228381346089744E-5
44-45	9.281596370008014E-5	8.07095336522436E-6	8.07095336522436E-6	1.3494432252821	3.228381346089744E-5
46-47	9.685144038269232E-5	8.07095336522436E-6	8.07095336522436E-6	1.6459781228738082	4.439024350873398E-5
48-49	9.685144038269232E-5	8.07095336522436E-6	8.07095336522436E-6	1.953223175581169	4.842572019134616E-5
50-51	1.0895787043052886E-4	8.07095336522436E-6	8.07095336522436E-6	2.2924614874282794	4.842572019134616E-5
52-53	1.1299334711314104E-4	8.07095336522436E-6	8.07095336522436E-6	2.642486628448012	6.456762692179488E-5
54-55	1.1299334711314104E-4	8.07095336522436E-6	8.07095336522436E-6	2.9844932772993946	8.07095336522436E-5
56-57	1.1299334711314104E-4	8.07095336522436E-6	8.07095336522436E-6	3.373271100902252	8.07095336522436E-5
58-59	1.1299334711314104E-4	8.07095336522436E-6	8.07095336522436E-6	3.7951035140123857	8.07095336522436E-5
60-61	1.1299334711314104E-4	8.07095336522436E-6	8.07095336522436E-6	4.211556636704597	8.474501033485578E-5
62-63	1.1299334711314104E-4	8.07095336522436E-6	8.07095336522436E-6	4.63341729815151	9.685144038269232E-5
64-65	1.1299334711314104E-4	8.07095336522436E-6	8.07095336522436E-6	5.078699866264303	9.685144038269232E-5
66-67	1.1299334711314104E-4	8.07095336522436E-6	8.07095336522436E-6	5.549143631493183	1.0088691706530451E-4
68-69	1.1299334711314104E-4	8.07095336522436E-6	8.07095336522436E-6	6.071439236616947	1.0492239374791669E-4
70-71	1.1299334711314104E-4	8.07095336522436E-6	8.07095336522436E-6	6.684952756674477	1.0492239374791669E-4
72-73	1.2913525384358976E-4	8.07095336522436E-6	8.07095336522436E-6	7.249834747229847	1.1299334711314104E-4
74-75	1.2913525384358976E-4	8.07095336522436E-6	8.07095336522436E-6	7.802182547209024	1.1299334711314104E-4
76-77	1.3720620720881414E-4	8.07095336522436E-6	8.07095336522436E-6	8.397379038604178	1.1299334711314104E-4
78-79	1.3720620720881414E-4	8.07095336522436E-6	8.07095336522436E-6	9.010133889045376	1.1299334711314104E-4
80-81	1.452771605740385E-4	8.07095336522436E-6	8.07095336522436E-6	9.668897209145037	1.210643004783654E-4
82-83	1.4931263725665068E-4	1.210643004783654E-5	8.07095336522436E-6	10.385238710552207	1.210643004783654E-4
84-85	1.5334811393926285E-4	1.614190673044872E-5	8.07095336522436E-6	11.072996930616435	1.210643004783654E-4
86-87	1.6141906730448723E-4	1.614190673044872E-5	8.07095336522436E-6	11.768329740413927	1.210643004783654E-4
88	1.6949002066971158E-4	1.614190673044872E-5	8.07095336522436E-6	12.314636431799233	1.210643004783654E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	4295	0.0	23.62376	1
TAGTACT	5115	0.0	20.386267	4
TACATGA	4525	0.0	20.24257	2
GTATAAG	4750	0.0	20.174137	1
GTACATA	3180	0.0	18.760094	1
CTAGTAC	5825	0.0	18.546963	3
CATGGGG	30620	0.0	18.438673	4
GTACAAG	6765	0.0	18.053606	1
TAGGCAT	6830	0.0	16.985098	5
GTGATCG	3485	0.0	16.441183	8
GTATATA	2925	0.0	16.380709	1
GTATTAG	4715	0.0	16.338816	1
CTAGGCA	7500	0.0	16.095417	4
TGATCGC	3565	0.0	16.072237	9
CTATTGA	5225	0.0	15.819852	9
ATTTAGG	5470	0.0	15.801157	1
TACTAGG	6515	0.0	15.645699	2
GTACAAA	5065	0.0	15.580746	1
CTGTGCG	6875	0.0	15.370424	9
ATAAGGT	4480	0.0	15.203053	3
>>END_MODULE