FastQCFastQC Report
Fri 27 May 2016
ERR765341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765341_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12310902
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1012750.8226448395089165No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA995400.8085516398392254No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG526020.4272798207637426No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG414620.33679091913817527No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG391470.3179864481091637No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC346780.2816852899974348No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC306790.24920188626308618No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT282880.22978007622837057No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC268560.21814810969984166No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT252610.2051921134617106No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA211180.17153901476918587No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC199860.16234391273685714No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG198940.16159660762468908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197980.160816810985905No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA132460.10759569038889272No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC132190.10737637258423469No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT131650.10693773697491867No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA131340.10668592764364462No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC126620.10285192750295633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC111700.022.6781143
TACACTC108450.022.1527085
CTACACT143000.021.2674544
GTATAAG48050.020.1489411
ATCTACA113650.019.7241082
TAGTACT53000.019.3342744
CTATTGA72300.018.9250859
AGGGCTA75150.018.7058585
ATTTAGG76450.018.6885011
GTACATA27000.018.4510271
GTACTAG53500.018.4477711
GAACAAA82200.018.3531931
TTTAGGG78350.018.2340452
TATTCGC11350.018.2280399
GTGATCG38950.018.1078538
GTACAAA49150.017.7855721
GGGCTAT87050.017.3376876
TGATCGC41000.017.3171429
TAGGCAT71150.017.3125825
GTCCTAC44050.017.284131
Produced by FastQC (version 0.11.5)