FastQCFastQC Report
Fri 27 May 2016
ERR765339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765339_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12660572
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA246350.19458046603265633No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT235450.18597106039126826No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG39950.021.1662831
GTATAAG47300.019.1683651
CATGGGG253750.019.0861874
GTATATA35250.017.3249971
TCTAACG22350.017.025232
GTACATG771350.016.589931
TACATGG765600.016.248062
TAGTACT49700.016.1624184
ACATGGG697150.015.9835133
CTAACGC22550.015.8325483
CGTTAAC12200.015.7874851
GTGATCG32750.015.6321988
CTAGTAC52250.015.5540523
GTATAAA119150.015.2582891
GTATTAG43200.015.2241551
TGATCGC33350.015.2101259
GTATTAC26850.014.87181
GTATCAA541450.014.871041
GTACATA31200.014.6051511
TACGGGC31200.014.6044014
Produced by FastQC (version 0.11.5)