##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765339_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12660572
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.860972790170933	33.0	33.0	33.0	27.0	33.0
2	31.777021843878774	33.0	33.0	33.0	27.0	33.0
3	31.78667180282218	33.0	33.0	33.0	27.0	33.0
4	35.558464025164106	37.0	37.0	37.0	33.0	37.0
5	35.59790094791926	37.0	37.0	37.0	33.0	37.0
6	35.647054888199364	37.0	37.0	37.0	33.0	37.0
7	35.46830901479017	37.0	37.0	37.0	33.0	37.0
8	35.45777599937823	37.0	37.0	37.0	33.0	37.0
9	35.37044392623019	37.0	37.0	37.0	33.0	37.0
10-11	35.314759988727204	37.0	37.0	37.0	33.0	37.0
12-13	35.26559463506072	37.0	37.0	37.0	33.0	37.0
14-15	36.887272549771055	40.0	37.0	40.0	33.0	40.0
16-17	36.90516273672311	40.0	37.0	40.0	33.0	40.0
18-19	36.84051779019147	40.0	37.0	40.0	33.0	40.0
20-21	36.55317919285163	40.0	37.0	40.0	33.0	40.0
22-23	36.580619619713865	40.0	37.0	40.0	33.0	40.0
24-25	36.461375165355875	37.0	37.0	40.0	33.0	40.0
26-27	36.43888289565432	37.0	37.0	40.0	33.0	40.0
28-29	36.37033425503998	37.0	37.0	40.0	33.0	40.0
30-31	36.49406405176639	37.0	37.0	40.0	33.0	40.0
32-33	36.30309444154656	37.0	37.0	40.0	33.0	40.0
34-35	36.144740932716154	37.0	37.0	40.0	27.0	40.0
36-37	36.25512543983005	37.0	37.0	40.0	33.0	40.0
38-39	36.19432095959014	37.0	37.0	40.0	33.0	40.0
40-41	36.24901248537586	37.0	37.0	40.0	33.0	40.0
42-43	36.183293100817245	37.0	37.0	40.0	33.0	40.0
44-45	36.1125253661525	37.0	37.0	40.0	30.0	40.0
46-47	35.95014834242876	37.0	37.0	40.0	27.0	40.0
48-49	35.77085300727329	37.0	37.0	40.0	27.0	40.0
50-51	35.585252230309976	37.0	35.0	40.0	27.0	40.0
52-53	35.44120040547931	37.0	33.0	40.0	27.0	40.0
54-55	35.30706136342023	37.0	33.0	40.0	27.0	40.0
56-57	35.15217938020494	37.0	33.0	40.0	27.0	40.0
58-59	34.971030376826576	37.0	33.0	40.0	27.0	40.0
60-61	34.748466854420165	37.0	33.0	38.5	27.0	40.0
62-63	34.526892031418484	37.0	33.0	37.0	27.0	40.0
64-65	34.32758436980572	37.0	33.0	37.0	27.0	40.0
66-67	34.0669945639107	37.0	33.0	37.0	27.0	40.0
68-69	33.85098508977319	37.0	33.0	37.0	27.0	40.0
70-71	33.554389762168725	37.0	33.0	37.0	27.0	38.5
72-73	33.34148982368254	37.0	33.0	37.0	27.0	37.0
74-75	33.1401457216941	37.0	33.0	37.0	27.0	37.0
76-77	32.882882305791554	37.0	33.0	37.0	27.0	37.0
78-79	32.79080175050542	35.0	33.0	37.0	27.0	37.0
80-81	32.63125109987132	33.0	33.0	37.0	27.0	37.0
82-83	32.48467399419236	33.0	33.0	37.0	22.0	37.0
84-85	32.3391683645889	33.0	33.0	37.0	22.0	37.0
86-87	32.184875256820945	33.0	33.0	37.0	22.0	37.0
88-89	32.06021714500735	33.0	33.0	37.0	22.0	37.0
90-91	31.948041447100493	33.0	33.0	37.0	22.0	37.0
92-93	31.83794871195393	33.0	33.0	37.0	22.0	37.0
94-95	31.712031099384767	33.0	33.0	37.0	22.0	37.0
96-97	31.588156759426035	33.0	33.0	37.0	22.0	37.0
98-99	31.48515268504456	33.0	33.0	37.0	22.0	37.0
100	31.276202686576877	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	21.0
7	316.0
8	1494.0
9	2634.0
10	3995.0
11	6730.0
12	11787.0
13	18928.0
14	26162.0
15	31178.0
16	35615.0
17	40785.0
18	47842.0
19	55683.0
20	63716.0
21	74828.0
22	86872.0
23	96987.0
24	104932.0
25	119006.0
26	137262.0
27	164545.0
28	201341.0
29	250366.0
30	313749.0
31	396475.0
32	519518.0
33	693514.0
34	937335.0
35	1331105.0
36	2163004.0
37	3370284.0
38	1351442.0
39	1121.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.46342662268754	18.231780578774824	12.646190139392493	25.658602659145142
2	16.015545794366094	20.574030400113365	37.388351096656706	26.02207270886383
3	18.15149368470876	25.205941309215135	29.234858246404738	27.40770675967137
4	13.3797501071132	17.135774039804403	36.31108296186012	33.17339289122228
5	14.622211811660293	36.80023479064961	33.56051093036585	15.017042467324243
6	32.36289697425245	36.22747821932851	17.391362043740962	14.018262762678082
7	28.153894784361434	31.328925337506686	22.12379536388561	18.393384514246268
8	27.101330947992775	32.90438173621997	20.1806573188869	19.813629996900364
9	27.237469952632722	14.450894902675874	19.234690899445965	39.07694424524544
10-11	25.501231523154043	24.751089832926972	27.319818824494984	22.427859819423997
12-13	26.452282272386483	22.6411782138584	26.868823482763492	24.037716030991625
14-15	24.234549113816282	23.570842863140705	24.2785639787953	27.916044044247716
16-17	24.190867954209207	26.184813824533393	25.115779858992365	24.508538362265035
18-19	24.49927886298618	25.604267952626873	26.47676889860208	23.419684285784868
20-21	25.326962367618172	25.00054404370486	25.88074949040581	23.79174409827116
22-23	24.87865908753024	24.73553123865922	25.638642410017503	24.747167263793035
24-25	24.559416724878623	25.26277543762462	25.677339113394893	24.500468724101868
26-27	24.75108425917078	25.21360959417031	25.50487592205335	24.530430224605556
28-29	24.45857773023402	25.06150934342719	25.99611082462915	24.483802101709642
30-31	24.845173620461008	25.052520078836217	25.712556905311835	24.38974939539094
32-33	24.42745214530553	25.1027052832553	25.586246896678094	24.88359567476108
34-35	24.731117836710183	25.1388287448241	25.863296930808865	24.266756487656856
36-37	24.91845163819943	25.093185851641227	25.380541571756886	24.607820938402455
38-39	24.896065675903817	24.834033024761062	25.55937251721228	24.710528782122836
40-41	24.735001067953025	24.979318534551936	25.721595274487637	24.564085123007406
42-43	25.077045748555616	24.956935144699337	25.39651356035204	24.569505546393007
44-45	24.86216739990636	25.157955041574393	25.19753220558632	24.782345352932925
46-47	24.627308489761354	25.057824356511908	25.58289796156401	24.731969192162722
48-49	25.157199529222275	25.17966210828433	25.17600329593403	24.487135066559368
50-51	24.491377045894996	25.464365494781088	25.136794705763165	24.907462753560747
52-53	24.54512054201934	25.295758066587	24.933422879774028	25.225698511619637
54-55	24.69826270473186	25.18838939878269	24.994193846097083	25.11915405038837
56-57	24.844235834122536	24.88117737330725	24.89235226791306	25.382234524657154
58-59	24.5457467820595	24.91654791966069	25.398173451553397	25.13953184672642
60-61	24.62010137213623	25.064482877873694	25.20915236769688	25.106263382293196
62-63	24.303228819763035	25.101190458320843	25.121233413605225	25.474347308310897
64-65	24.230888513552348	24.903235317668194	25.288745543460156	25.577130625319306
66-67	24.76098998120439	24.847422931277904	25.140777469916355	25.250809617601348
68-69	24.634441751177995	24.59726492998385	25.223369055105916	25.544924263732234
70-71	24.176242696726153	24.736676044685026	25.284831679785874	25.80224957880295
72-73	24.709076561618836	24.719821364492	25.22415949964613	25.34694257424303
74-75	24.789200632111722	24.660656291108257	25.1813276289593	25.368815447820726
76-77	24.408811702713308	24.768133877482914	25.352744944737047	25.470309475066728
78-79	24.498349300719273	24.79190965764354	25.31249202539265	25.397249016244537
80-81	24.17340585478337	24.76155138577364	25.511122993397628	25.553919766045365
82-83	24.338719229507568	24.793359634979335	25.203954158784498	25.663966976728602
84-85	24.35807600884436	24.84715700413665	24.98004678162607	25.81472020539292
86-87	24.146119640707955	24.62941005764579	25.244914207635293	25.979556094010963
88-89	24.22439225458282	24.260491425143346	25.44771089660456	26.06740542366928
90-91	24.423736046887335	24.479852089094972	25.23842799707367	25.857983866944025
92-93	24.18272393093681	24.511487157809906	25.261519255877896	26.04426965537539
94-95	23.698577936786464	24.568054629501678	25.599847859849657	26.133519573862202
96-97	24.2360317736417	24.378437250115365	25.30885498799013	26.076675988252802
98-99	23.982294116522304	24.401356219538037	25.293540211172918	26.322809452766744
100	23.937572785629815	24.3950169865402	25.280534327318943	26.386875900511043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	544.0
1	615.5
2	932.5
3	1196.5
4	1041.0
5	932.5
6	1233.5
7	2390.5
8	3997.0
9	4079.0
10	2743.5
11	1884.5
12	1784.0
13	1906.5
14	2111.0
15	2489.0
16	2986.5
17	3515.5
18	4065.0
19	4830.5
20	5911.5
21	7367.5
22	9432.5
23	12294.5
24	15969.5
25	20486.5
26	26690.0
27	33692.5
28	41149.0
29	52467.5
30	65547.5
31	78443.0
32	94014.5
33	110396.5
34	127671.5
35	144245.5
36	161658.5
37	184935.0
38	204922.0
39	223382.5
40	240330.5
41	255931.0
42	276297.5
43	309273.0
44	350660.0
45	391519.5
46	440427.0
47	486837.0
48	532806.0
49	569968.5
50	605150.0
51	612022.0
52	590574.0
53	588005.5
54	578023.5
55	551405.0
56	519283.0
57	475117.5
58	439438.5
59	406006.5
60	352984.5
61	293692.0
62	240139.0
63	191616.5
64	147893.0
65	115403.5
66	88811.0
67	66722.5
68	51664.0
69	41143.0
70	34632.5
71	30001.0
72	25168.5
73	23802.0
74	19584.0
75	14832.0
76	11256.0
77	7785.5
78	5668.5
79	3822.5
80	2651.0
81	2015.5
82	1493.0
83	1017.5
84	686.0
85	436.5
86	277.0
87	182.5
88	124.0
89	73.0
90	54.0
91	47.0
92	37.0
93	28.5
94	20.0
95	15.5
96	8.5
97	3.5
98	6.0
99	5.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016926565403206112
2	0.005410498040688841
3	0.009004332505671939
4	0.008435637821103186
5	0.019643662229479046
6	0.0039018774191245074
7	0.024635537794026996
8	0.008088102180533392
9	0.0011452879064231852
10-11	2.4090538721315276E-4
12-13	9.478244742812567E-4
14-15	6.042381023543011E-4
16-17	9.715200861382883E-4
18-19	0.0028158285423439005
20-21	0.005872562471900954
22-23	0.012716645029940195
24-25	0.022163295623610052
26-27	0.027072236546658395
28-29	0.032869762914345416
30-31	0.03522747629412004
32-33	0.03615555442518711
34-35	0.04559035721292845
36-37	0.04226902228430121
38-39	0.046964702700636275
40-41	0.04360782435422349
42-43	0.050566435702905047
44-45	0.043813186323651096
46-47	0.04636441386692481
48-49	0.048911692141555684
50-51	0.04903411946948369
52-53	0.03315016098798696
54-55	0.029560275791646697
56-57	0.02159855020768414
58-59	0.02139713750689937
60-61	0.02175652095339768
62-63	0.021784165833897553
64-65	0.025200283209952917
66-67	0.026554882354446543
68-69	0.027581692201584573
70-71	0.027577742932941733
72-73	0.025986187669877793
74-75	0.021191775537471765
76-77	0.025812419849592892
78-79	0.025346406149737943
80-81	0.024291951422100045
82-83	0.0261915496393054
84-85	0.027376330232156968
86-87	0.02995915192457339
88-89	0.029789333372931333
90-91	0.029726145074645913
92-93	0.031965380395135384
94-95	0.030180310968572353
96-97	0.03243534336363318
98-99	0.033284436121843466
100	0.0415305090480904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2660572E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.33360810369182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.35327146514831	28.779348881916277
2	14.459777426649664	11.66429991986194
3	5.512774557495846	6.670502656981217
4	2.6417823235248514	4.262104517292471
5	1.5120844464814835	3.049391074203596
6	0.921630819574645	2.230361777580482
7	0.6186881407609807	1.7467747505498583
8	0.4572616528402471	1.4754409845203924
9	0.33652157056824783	1.2215816231164718
>10	1.8983905647538226	14.079135100660523
>50	0.15513250982243032	4.328457039856002
>100	0.10895534739359448	8.823418655746393
>500	0.014721502042941608	4.092194435372782
>1k	0.008667384896139206	6.3501149080870425
>5k	2.8023721503708576E-4	0.7412250770565483
>10k+	6.005083179366124E-5	0.48564859719834164
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	24635	0.19458046603265633	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	23545	0.18597106039126826	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.579707457135428E-5
2	0.0	0.0	0.0	0.0	1.579707457135428E-5
3	0.0	0.0	0.0	0.0	1.579707457135428E-5
4	0.0	0.0	0.0	0.0	2.369561185703142E-5
5	0.0	2.369561185703142E-5	0.0	1.579707457135428E-5	2.369561185703142E-5
6	0.0	2.369561185703142E-5	0.0	1.579707457135428E-5	4.739122371406284E-5
7	0.0	3.159414914270856E-5	0.0	3.159414914270856E-5	4.739122371406284E-5
8	0.0	3.94926864283857E-5	0.0	3.159414914270856E-5	4.739122371406284E-5
9	0.0	3.94926864283857E-5	0.0	6.318829828541712E-5	6.318829828541712E-5
10-11	7.89853728567714E-6	3.94926864283857E-5	0.0	7.89853728567714E-5	6.318829828541712E-5
12-13	1.184780592851571E-5	4.344195507122427E-5	0.0	7.89853728567714E-5	9.478244742812568E-5
14-15	1.579707457135428E-5	5.134049235690141E-5	0.0	7.89853728567714E-5	1.2242732792799567E-4
16-17	1.579707457135428E-5	5.528976099973998E-5	0.0	8.293464149960997E-5	1.5007220842786566E-4
18-19	1.579707457135428E-5	6.318829828541712E-5	0.0	8.688391014244854E-5	1.698185516420585E-4
20-21	2.369561185703142E-5	9.478244742812568E-5	0.0	1.5007220842786566E-4	1.7376782028489707E-4
22-23	2.369561185703142E-5	9.478244742812568E-5	0.0	6.042381023543012E-4	1.816663575705742E-4
24-25	4.344195507122427E-5	9.478244742812568E-5	0.0	0.001552062576635558	2.0536196942760564E-4
26-27	5.528976099973998E-5	9.478244742812568E-5	0.0	0.002780285124558353	2.1326050671328277E-4
28-29	6.318829828541712E-5	9.478244742812568E-5	0.0	0.008858209565886913	2.1326050671328277E-4
30-31	7.108683557109427E-5	9.478244742812568E-5	0.0	0.028948139152006717	2.1720977535612135E-4
32-33	7.89853728567714E-5	1.0268098471380282E-4	0.0	0.06841318069989255	2.2905758128463706E-4
34-35	8.293464149960997E-5	1.0268098471380282E-4	0.0	0.11881374711979838	2.527531931416685E-4
36-37	8.688391014244854E-5	1.0268098471380282E-4	0.0	0.19637343399650503	2.6460099907018415E-4
38-39	8.688391014244854E-5	1.0268098471380282E-4	0.0	0.3209017728424909	2.6855026771302275E-4
40-41	8.688391014244854E-5	1.0663025335664139E-4	0.0	0.47261687702577737	2.6855026771302275E-4
42-43	8.688391014244854E-5	1.1057952199947996E-4	0.0	0.6096446511263471	2.7249953635586136E-4
44-45	8.688391014244854E-5	1.1057952199947996E-4	0.0	0.7685474242395999	2.9224587957005417E-4
46-47	8.688391014244854E-5	1.1057952199947996E-4	0.0	0.947769184520257	3.198907600699242E-4
48-49	9.08331787852871E-5	1.1057952199947996E-4	0.0	1.1363823056335844	3.3963710328411705E-4
50-51	9.478244742812568E-5	1.1057952199947996E-4	0.0	1.3417047823747614	3.4753564056979415E-4
52-53	9.478244742812568E-5	1.1452879064231854E-4	0.0	1.545435703852875	3.988761329266956E-4
54-55	9.478244742812568E-5	1.3822440249934995E-4	0.0	1.7540558199108225	4.5021662528359695E-4
56-57	9.478244742812568E-5	1.5007220842786566E-4	0.0	1.989554658351929	4.6206443121211266E-4
58-59	9.478244742812568E-5	1.7376782028489707E-4	0.0	2.251225300089127	4.6601369985495127E-4
60-61	9.478244742812568E-5	1.7771708892773565E-4	0.0	2.51916343116251	4.7786150578346697E-4
62-63	9.478244742812568E-5	1.8166635757057423E-4	0.0	2.7887247116480993	5.05506386283337E-4
64-65	9.478244742812568E-5	1.856156262134128E-4	0.0	3.0700074214656334	5.173541922118526E-4
66-67	9.478244742812568E-5	2.0536196942760564E-4	0.0	3.376048096405123	5.213034608546912E-4
68-69	9.478244742812568E-5	2.0536196942760564E-4	0.0	3.7095322391436976	5.213034608546912E-4
70-71	9.478244742812568E-5	2.0931123807044422E-4	0.0	4.0871494589659925	5.213034608546912E-4
72-73	9.478244742812568E-5	2.1326050671328277E-4	0.0	4.458826978749459	5.607961472830769E-4
74-75	9.478244742812568E-5	2.1326050671328277E-4	0.0	4.83340326171677	5.607961472830769E-4
76-77	9.478244742812568E-5	2.1326050671328277E-4	0.0	5.235853482765234	5.686946845687541E-4
78-79	9.478244742812568E-5	2.1326050671328277E-4	0.0	5.659029465651315	5.765932218544312E-4
80-81	1.0268098471380282E-4	2.1720977535612135E-4	0.0	6.120138173851861	5.923902964257855E-4
82-83	1.0268098471380282E-4	2.2115904399895993E-4	0.0	6.611115200798194	6.239844455684941E-4
84-85	1.0268098471380282E-4	2.2115904399895993E-4	0.0	7.106914284757434	6.239844455684941E-4
86-87	1.0268098471380282E-4	2.2115904399895993E-4	0.0	7.616026353311683	6.358322514970098E-4
88	1.0268098471380282E-4	2.2115904399895993E-4	0.0	8.011833904502893	6.713756692825569E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	3995	0.0	21.166283	1
GTATAAG	4730	0.0	19.168365	1
CATGGGG	25375	0.0	19.086187	4
GTATATA	3525	0.0	17.324997	1
TCTAACG	2235	0.0	17.02523	2
GTACATG	77135	0.0	16.58993	1
TACATGG	76560	0.0	16.24806	2
TAGTACT	4970	0.0	16.162418	4
ACATGGG	69715	0.0	15.983513	3
CTAACGC	2255	0.0	15.832548	3
CGTTAAC	1220	0.0	15.787485	1
GTGATCG	3275	0.0	15.632198	8
CTAGTAC	5225	0.0	15.554052	3
GTATAAA	11915	0.0	15.258289	1
GTATTAG	4320	0.0	15.224155	1
TGATCGC	3335	0.0	15.210125	9
GTATTAC	2685	0.0	14.8718	1
GTATCAA	54145	0.0	14.87104	1
GTACATA	3120	0.0	14.605151	1
TACGGGC	3120	0.0	14.604401	4
>>END_MODULE