FastQCFastQC Report
Fri 27 May 2016
ERR765338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765338_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9241244
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123680.13383479540200433No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT94090.1018152967284491No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA296550.029.2339741
CAACGCA338050.024.1151075
ATCAACG337350.024.0824893
AACGCAG356250.023.144656
GTACTAG37600.022.994261
TATCAAC380650.022.7133622
ACGCAGA372200.021.5974257
TCAACGC379400.021.5490074
CGCAGAG372450.021.4063288
GTACATG495300.020.4915521
TACATGG487750.020.1825582
TAGGCAT54000.020.0996675
GCAGAGT383050.020.0535229
CTATTGA39200.019.7754449
CATGGGG230000.019.4688554
ACATGGG487800.018.870343
GTACAAG39500.018.0815621
ATTTAGG44100.017.9002911
TTTAGGG46950.017.7144282
AGAGTAC359400.017.41886310-11
Produced by FastQC (version 0.11.5)