##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765338_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9241244
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.945051445454745	33.0	33.0	33.0	27.0	33.0
2	31.85701741021014	33.0	33.0	33.0	27.0	33.0
3	31.87170233791035	33.0	33.0	33.0	27.0	33.0
4	35.644683443051605	37.0	37.0	37.0	33.0	37.0
5	35.692955840144464	37.0	37.0	37.0	33.0	37.0
6	35.66376669634521	37.0	37.0	37.0	33.0	37.0
7	35.605781321216064	37.0	37.0	37.0	33.0	37.0
8	35.601121017906245	37.0	37.0	37.0	33.0	37.0
9	35.5780006458005	37.0	37.0	37.0	33.0	37.0
10-11	35.556237937230094	37.0	37.0	37.0	33.0	37.0
12-13	35.50912117459511	37.0	37.0	37.0	33.0	37.0
14-15	37.11451883534295	40.0	37.0	40.0	33.0	40.0
16-17	37.16287049665608	40.0	37.0	40.0	33.0	40.0
18-19	37.134701507719086	40.0	37.0	40.0	33.0	40.0
20-21	37.22288043687625	40.0	37.0	40.0	33.0	40.0
22-23	37.20913245013334	40.0	37.0	40.0	33.0	40.0
24-25	37.18960412689027	40.0	37.0	40.0	33.0	40.0
26-27	37.14631753041041	40.0	37.0	40.0	33.0	40.0
28-29	37.10612250904748	40.0	37.0	40.0	33.0	40.0
30-31	37.0339010094312	40.0	37.0	40.0	33.0	40.0
32-33	36.933405448443956	40.0	37.0	40.0	33.0	40.0
34-35	36.8250136561701	37.0	37.0	40.0	33.0	40.0
36-37	36.74583140538222	37.0	37.0	40.0	33.0	40.0
38-39	36.647603720884334	37.0	37.0	40.0	33.0	40.0
40-41	36.546288573270004	37.0	37.0	40.0	33.0	40.0
42-43	36.40981722807016	37.0	37.0	40.0	33.0	40.0
44-45	36.25714779308933	37.0	37.0	40.0	33.0	40.0
46-47	36.07919480321048	37.0	37.0	40.0	27.0	40.0
48-49	35.8313939119019	37.0	37.0	40.0	27.0	40.0
50-51	35.725937871567936	37.0	37.0	40.0	27.0	40.0
52-53	35.56828523302707	37.0	35.0	40.0	27.0	40.0
54-55	35.40854505086112	37.0	33.0	40.0	27.0	40.0
56-57	35.241345375146466	37.0	33.0	40.0	27.0	40.0
58-59	35.06300288143025	37.0	33.0	40.0	27.0	40.0
60-61	34.872986526489285	37.0	33.0	40.0	27.0	40.0
62-63	34.65544692900653	37.0	33.0	37.0	27.0	40.0
64-65	34.44403188575045	37.0	33.0	37.0	27.0	40.0
66-67	34.24495089622133	37.0	33.0	37.0	27.0	40.0
68-69	34.04334503017127	37.0	33.0	37.0	27.0	40.0
70-71	33.85936931218351	37.0	33.0	37.0	27.0	40.0
72-73	33.65704725467697	37.0	33.0	37.0	27.0	37.0
74-75	33.44325141723344	37.0	33.0	37.0	27.0	37.0
76-77	33.26320531088672	37.0	33.0	37.0	27.0	37.0
78-79	33.108537551870725	37.0	33.0	37.0	27.0	37.0
80-81	32.95698631050105	37.0	33.0	37.0	27.0	37.0
82-83	32.834985798448784	37.0	33.0	37.0	27.0	37.0
84-85	32.7066290534045	37.0	33.0	37.0	27.0	37.0
86-87	32.59912328902905	33.0	33.0	37.0	24.5	37.0
88-89	32.498004868175755	33.0	33.0	37.0	22.0	37.0
90-91	32.38717639097074	33.0	33.0	37.0	22.0	37.0
92-93	32.300616399696835	33.0	33.0	37.0	22.0	37.0
94-95	32.201461188558596	33.0	33.0	37.0	22.0	37.0
96-97	32.12347552991783	33.0	33.0	37.0	22.0	37.0
98-99	32.030316589411555	33.0	33.0	37.0	22.0	37.0
100	31.981153078524926	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	2.0
6	12.0
7	162.0
8	1074.0
9	2298.0
10	3171.0
11	4363.0
12	6860.0
13	11237.0
14	17236.0
15	20758.0
16	23599.0
17	27551.0
18	33794.0
19	40176.0
20	47199.0
21	55913.0
22	66043.0
23	67930.0
24	66913.0
25	72979.0
26	83158.0
27	99389.0
28	123370.0
29	156197.0
30	201516.0
31	261977.0
32	346892.0
33	461874.0
34	613151.0
35	852753.0
36	1442426.0
37	2669213.0
38	1358475.0
39	1582.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.56958693206649	19.368221157769486	13.045713913403553	25.01647799676046
2	16.10480422686776	21.048814937749277	37.80650288679554	25.039877948587424
3	17.973175676194828	26.33762607212507	29.489820134810962	26.199378116869138
4	13.068291447166445	17.460113913405856	36.81148730548252	32.660107333945184
5	14.06889094416153	37.494076776666574	33.53558269848252	14.901449580689377
6	31.136381638662503	37.483795471691906	17.539489272223523	13.84033361742207
7	26.90506819211786	32.157358901031074	22.52999704368806	18.40757586316301
8	26.564778508174875	33.010977742823364	20.256223079923004	20.16802066907875
9	26.886639937220576	14.735948969640884	20.06246128767945	38.314949805459094
10-11	25.370653561360353	25.57755752364076	27.18736784787849	21.8644210671204
12-13	26.063758299207336	23.627700989174187	26.957236493268656	23.351304218349824
14-15	23.941890290960828	24.30484467242722	24.802028817765226	26.951236218846724
16-17	23.514004118508293	26.801630955253696	25.389483628226174	24.29488129801184
18-19	23.75343100202844	26.1366254360274	27.28354996507452	22.826393596869636
20-21	25.357413578453414	25.334137342775847	26.033555220461444	23.274893858309298
22-23	24.797026798560537	24.970729038087395	26.08609620787174	24.146147955480327
24-25	24.105565332187517	25.544231760990105	26.392783173638122	23.957419733184253
26-27	24.19605770259347	25.65438428366335	26.099235574338103	24.050322439405075
28-29	23.853112108779438	25.591361468749028	26.54320697025	24.012319452221533
30-31	24.24000722125048	25.361005701995488	26.40163097223769	23.997356104516346
32-33	24.020031271467342	25.626802445115977	25.825508026063705	24.527658257352975
34-35	24.38924981624748	25.475812551924328	26.344393909984614	23.790543721843576
36-37	24.34029626995356	25.383497588046083	26.014340446702544	24.261865695297814
38-39	24.37282857866471	25.113081670335756	26.266286336469918	24.24780341452961
40-41	24.206244937308544	25.193646679759286	26.529518004442153	24.07059037849002
42-43	24.47717251306179	25.246785872976968	26.125126418622717	24.15091519533852
44-45	24.16056424321408	25.565279232174497	25.87934775179745	24.394808772813974
46-47	24.011179495549264	25.455101165700572	26.000850253809855	24.532869084940312
48-49	24.582455159495524	25.49323432094104	25.660311239472534	24.263999280090903
50-51	24.020485204664013	25.70584213600654	25.529749892522744	24.743922766806694
52-53	24.011326815916835	25.65063354931956	25.380733296759256	24.957306338004347
54-55	24.107486849024625	25.627723962459605	25.45445758548303	24.810331603032747
56-57	24.19083195040371	25.268423988365424	25.334760238206606	25.20598382302426
58-59	23.901750616600644	25.21393653285638	25.81740915063606	25.066903699906913
60-61	23.982426684097266	25.401570539619268	25.541055731249873	25.074947045033596
62-63	23.866618539261086	25.290958848362415	25.374638841522117	25.46778377085438
64-65	23.715033824632243	25.212495982920778	25.563012016958233	25.509458175488746
66-67	24.14905994043187	25.224717094668307	25.405856594919392	25.220366369980436
68-69	24.09789330191962	24.955628996346054	25.502528231407855	25.443949470326473
70-71	23.67386425856766	24.98734771877733	25.580063738386162	25.75872428426885
72-73	24.201964276583084	25.098547963417843	25.508199276051446	25.19128848394762
74-75	24.286516462415896	25.19713388092624	25.482370385311953	25.033979271345906
76-77	23.9094066406373	25.31755822058529	25.489392505174724	25.28364263360269
78-79	23.94213428520876	25.416951412256967	25.478878505481994	25.16203579705228
80-81	23.763210657173925	25.222475344723698	25.784420644292233	25.22989335381014
82-83	23.948798327147554	25.121354357754544	25.721280460597118	25.20856685450078
84-85	23.98166693701954	25.215206477993906	25.322997737584718	25.48012884740184
86-87	23.59664574477456	25.100735436863264	25.68578876095954	25.61683005740264
88-89	23.77398890267746	24.665815329437425	25.69915014887761	25.861045619007513
90-91	23.925286536962705	24.83458132612501	25.5362779993556	25.703854137556682
92-93	23.838337252673224	24.829281064310305	25.49978279193816	25.832598891078312
94-95	23.239719423393865	24.890595900646794	25.95442361570268	25.91526106025666
96-97	23.677942629773945	24.813075907756826	25.57961198956728	25.929369472901953
98-99	23.370174265345607	24.94440584866704	25.57787076835175	26.107549117635603
100	23.40862089693852	24.957924185512546	25.393588633854332	26.239866283694603
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	451.0
1	458.5
2	619.5
3	760.0
4	638.5
5	587.5
6	779.0
7	1468.5
8	2311.5
9	2284.5
10	1537.0
11	1108.5
12	1174.0
13	1332.5
14	1616.0
15	1968.5
16	2253.0
17	2649.5
18	3292.5
19	4091.0
20	5000.5
21	6181.5
22	7850.5
23	9933.5
24	12344.0
25	15854.5
26	21026.0
27	26758.5
28	33439.0
29	44262.0
30	56462.5
31	67968.5
32	81993.5
33	96612.5
34	113579.0
35	128217.5
36	141488.0
37	161911.5
38	177070.5
39	185440.0
40	192942.0
41	201618.0
42	214083.0
43	238018.5
44	270968.5
45	302653.5
46	340238.0
47	377933.0
48	405219.5
49	419778.0
50	428928.5
51	428772.0
52	420401.0
53	418772.5
54	408073.0
55	382340.0
56	358955.0
57	328256.0
58	303431.5
59	278241.0
60	231527.0
61	183798.5
62	147510.5
63	116264.0
64	87202.5
65	67117.5
66	51779.5
67	38161.0
68	29601.5
69	24306.0
70	20989.5
71	18507.5
72	15959.0
73	16278.5
74	13369.0
75	9932.5
76	7621.0
77	5233.0
78	3795.0
79	2457.5
80	1691.0
81	1222.5
82	889.5
83	617.0
84	420.0
85	282.0
86	203.0
87	126.5
88	56.0
89	39.5
90	32.5
91	18.0
92	13.5
93	12.0
94	7.0
95	4.5
96	6.0
97	6.0
98	3.5
99	4.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01785473903729844
2	0.0059299375711754825
3	0.006503453431161432
4	0.003949684696129655
5	0.04649806887470994
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	3.6791583470796786E-4
18-19	0.0015041265007178688
20-21	6.384421837579443E-4
22-23	0.004658463730640593
24-25	0.007277158789444365
26-27	0.005578253317410513
28-29	0.02201543428568708
30-31	0.019986486667812255
32-33	0.012893285795721876
34-35	0.013358591116087833
36-37	0.013255791103448843
38-39	0.020603286743646202
40-41	0.029265540440226445
42-43	0.017362381082027485
44-45	0.016669833628459543
46-47	0.01785473903729844
48-49	0.012860822633835879
50-51	0.009917495956172135
52-53	0.02662520327349868
54-55	0.024785624099958836
56-57	0.012963622646474868
58-59	0.01591777037810061
60-61	0.016810507329965533
62-63	0.020332760394596226
64-65	0.010934675028600046
66-67	0.015192759762646673
68-69	0.007504400922646344
70-71	0.00882456950601023
72-73	0.010242127575032105
74-75	0.0034194530519917016
76-77	0.007087790345109381
78-79	0.0058325480855174906
80-81	0.0023806318716397923
82-83	2.4347371414497874E-4
84-85	0.002542947681069778
86-87	0.002229137116171805
88-89	0.0010388211803519093
90-91	7.737053582829325E-4
92-93	0.0011308001390289012
94-95	0.0029649687855877412
96-97	0.0013688633261928805
98-99	0.0017746528497678451
100	0.0020235370908938234
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9241244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.62315133511811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.01185820433038	27.105474836104943
2	13.6884992904762	10.026319621315332
3	4.836743580404803	5.314103763429783
4	2.228397195703758	3.2644371093204607
5	1.2473040755966254	2.2840102960742406
6	0.764500332169018	1.6799046816464405
7	0.5089024560820309	1.304632816473386
8	0.34611229382509257	1.0140578332560983
9	0.27578419219200934	0.9090077585833128
>10	1.6948560710618281	11.722308217852305
>50	0.18597763636034173	4.775000882154518
>100	0.16823516791427293	12.828397907962364
>500	0.02693661450632862	6.901055905602019
>1k	0.01562503169495824	10.123531490858042
>5k	2.380957179544524E-4	0.6129485315685894
>10k+	2.976196474430655E-5	0.1348083477983518
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12368	0.13383479540200433	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	9409	0.1018152967284491	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.164210792399811E-5
2	0.0	4.328421584799622E-5	0.0	0.0	2.164210792399811E-5
3	0.0	4.328421584799622E-5	0.0	0.0	2.164210792399811E-5
4	0.0	1.2985264754398866E-4	0.0	0.0	2.164210792399811E-5
5	0.0	1.2985264754398866E-4	0.0	0.0	2.164210792399811E-5
6	0.0	1.2985264754398866E-4	0.0	0.0	2.164210792399811E-5
7	0.0	1.2985264754398866E-4	0.0	0.0	2.164210792399811E-5
8	0.0	1.514947554679868E-4	0.0	0.0	2.164210792399811E-5
9	0.0	2.705263490499764E-4	0.0	0.0	2.164210792399811E-5
10-11	0.0	2.705263490499764E-4	0.0	0.0	2.164210792399811E-5
12-13	5.410526980999528E-6	2.759368760309759E-4	0.0	0.0	3.2463161885997165E-5
14-15	1.0821053961999056E-5	4.112000505559641E-4	0.0	0.0	7.57473777339934E-5
16-17	1.0821053961999056E-5	4.2202110451796315E-4	0.0	0.0	8.656843169599245E-5
18-19	1.0821053961999056E-5	4.3825268546096173E-4	0.0	0.0	1.1903159358198961E-4
20-21	2.164210792399811E-5	4.5989479338495986E-4	0.0	7.033685075299386E-5	1.2985264754398866E-4
22-23	3.2463161885997165E-5	4.869474282899575E-4	0.0	3.138105648979726E-4	1.569052824489863E-4
24-25	6.492632377199433E-5	5.789263869669495E-4	0.0	0.0011470317199719	1.731368633919849E-4
26-27	1.6231580942998583E-4	6.276211297959452E-4	0.0	0.0019910739290078263	1.785473903729844E-4
28-29	1.731368633919849E-4	6.276211297959452E-4	0.0	0.010177201251260112	1.8395791735398395E-4
30-31	1.8936844433498347E-4	7.358316694159358E-4	0.0	0.04522659503417505	1.8395791735398395E-4
32-33	2.0560002527798206E-4	7.68294831301933E-4	0.0	0.11519552995246093	2.1642107923998112E-4
34-35	2.0560002527798206E-4	8.765053709219236E-4	0.0	0.2072177728453009	2.2724213320198016E-4
36-37	2.1642107923998112E-4	0.0010117685454469117	0.0	0.35316673815776317	2.2724213320198016E-4
38-39	2.2724213320198016E-4	0.0010712843422379065	0.0	0.5921821780703983	2.2724213320198016E-4
40-41	2.2724213320198016E-4	0.0011470317199719	0.0	0.8717819808675109	2.3806318716397922E-4
42-43	2.2724213320198016E-4	0.0012011369897818951	0.0	1.1204173377523632	2.3806318716397922E-4
44-45	2.3806318716397922E-4	0.0013850949071358791	0.0	1.4005798353554997	2.3806318716397922E-4
46-47	2.3806318716397922E-4	0.0015149475546798678	0.0	1.7151262319228882	2.488842411259783E-4
48-49	2.3806318716397922E-4	0.0015257686086418668	0.0	2.051076673227111	2.8134740301197545E-4
50-51	2.3806318716397922E-4	0.0017097265259958508	0.0	2.39983383189536	2.8134740301197545E-4
52-53	2.488842411259783E-4	0.001731368633919849	0.0	2.726867724734895	3.029895109359736E-4
54-55	2.705263490499764E-4	0.0017367791609008486	0.0	3.056839533725113	3.3545267282197074E-4
56-57	2.705263490499764E-4	0.001742189687881848	0.0	3.436902001505425	3.462737267839698E-4
58-59	2.8134740301197545E-4	0.0017476002148628473	0.0	3.8529931684522127	3.462737267839698E-4
60-61	2.86757929992975E-4	0.0017746528497678451	0.0	4.287085158664786	3.679158347079679E-4
62-63	2.921684569739745E-4	0.00183416864655884	0.0	4.723676812342581	3.7873688866996695E-4
64-65	3.029895109359736E-4	0.0020343581448558226	0.0	5.152520591383585	3.89557942631966E-4
66-67	3.0840003791697307E-4	0.0020560002527798206	0.0	5.614952921922633	3.9496846961296553E-4
68-69	3.138105648979726E-4	0.0021101055225898157	0.0	6.1335032383086086	4.003789965939651E-4
70-71	3.138105648979726E-4	0.0021479792114568125	0.0	6.7036375189314334	4.2202110451796315E-4
72-73	3.2463161885997165E-4	0.002153389738437812	0.0	7.269264830579086	4.5989479338495986E-4
74-75	3.2463161885997165E-4	0.0021858529003238093	0.0	7.820689508901616	4.653053203659594E-4
76-77	3.300421458409712E-4	0.002261600278057803	0.0	8.414727497726496	4.653053203659594E-4
78-79	3.3545267282197074E-4	0.0023048844939057987	0.0	9.04677984911988	4.653053203659594E-4
80-81	3.3545267282197074E-4	0.002315705547867798	0.0	9.728847111925624	4.7612637432795844E-4
82-83	3.3545267282197074E-4	0.0023319371288107963	0.0	10.449713263712114	5.085895362139557E-4
84-85	3.462737267839698E-4	0.0023589897637157943	0.0	11.145896591411287	5.085895362139557E-4
86-87	3.462737267839698E-4	0.0023806318716397923	0.0	11.854859583839579	5.085895362139557E-4
88	3.462737267839698E-4	0.0024996634652217817	0.0	12.403546535509722	5.194105901759546E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	29655	0.0	29.233974	1
CAACGCA	33805	0.0	24.115107	5
ATCAACG	33735	0.0	24.082489	3
AACGCAG	35625	0.0	23.14465	6
GTACTAG	3760	0.0	22.99426	1
TATCAAC	38065	0.0	22.713362	2
ACGCAGA	37220	0.0	21.597425	7
TCAACGC	37940	0.0	21.549007	4
CGCAGAG	37245	0.0	21.406328	8
GTACATG	49530	0.0	20.491552	1
TACATGG	48775	0.0	20.182558	2
TAGGCAT	5400	0.0	20.099667	5
GCAGAGT	38305	0.0	20.053522	9
CTATTGA	3920	0.0	19.775444	9
CATGGGG	23000	0.0	19.468855	4
ACATGGG	48780	0.0	18.87034	3
GTACAAG	3950	0.0	18.081562	1
ATTTAGG	4410	0.0	17.900291	1
TTTAGGG	4695	0.0	17.714428	2
AGAGTAC	35940	0.0	17.418863	10-11
>>END_MODULE