##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765336_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6553955 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47961131866178 34.0 31.0 34.0 31.0 34.0 2 32.588288445678984 34.0 31.0 34.0 31.0 34.0 3 32.63995389043715 34.0 31.0 34.0 31.0 34.0 4 36.1436415111181 37.0 35.0 37.0 35.0 37.0 5 36.078947597290494 37.0 35.0 37.0 35.0 37.0 6 36.164154773720604 37.0 37.0 37.0 35.0 37.0 7 36.10959031607632 37.0 36.0 37.0 35.0 37.0 8 36.14473138738365 37.0 36.0 37.0 35.0 37.0 9 37.86486815976002 39.0 38.0 39.0 35.0 39.0 10-11 37.816250874471976 39.0 38.0 39.0 35.0 39.0 12-13 37.78804767502981 39.0 38.0 39.0 35.0 39.0 14-15 39.10832817741348 40.5 38.5 41.0 36.0 41.0 16-17 39.05182588223447 40.0 38.5 41.0 36.0 41.0 18-19 39.07604972875157 40.0 38.5 41.0 35.5 41.0 20-21 39.0875520201161 40.0 39.0 41.0 35.5 41.0 22-23 39.053880900921655 40.0 39.0 41.0 35.5 41.0 24-25 39.003750636066314 40.0 39.0 41.0 35.0 41.0 26-27 38.83257063864491 40.0 38.0 41.0 35.0 41.0 28-29 38.682505830448946 40.0 38.0 41.0 35.0 41.0 30-31 38.46626960362102 40.0 38.0 41.0 34.0 41.0 32-33 38.106799634724375 40.0 37.0 41.0 34.0 41.0 34-35 38.10234377562861 40.0 38.0 41.0 34.0 41.0 36-37 38.037867669216524 40.0 38.0 41.0 33.5 41.0 38-39 37.86213355142048 40.0 37.0 41.0 33.0 41.0 40-41 37.594182978064396 40.0 37.0 41.0 33.0 41.0 42-43 37.32778902204852 40.0 36.5 41.0 32.5 41.0 44-45 36.93550787272723 40.0 36.0 41.0 31.5 41.0 46-47 36.58486394856236 39.0 35.5 41.0 31.0 41.0 48-49 36.325929457861704 39.0 35.0 41.0 30.5 41.0 50-51 36.11441053531799 39.0 35.0 41.0 30.0 41.0 52-53 36.00720244493592 39.0 35.0 41.0 30.0 41.0 54-55 35.983455791197834 39.0 35.0 41.0 30.0 41.0 56-57 35.798475119221905 39.0 35.0 41.0 29.0 41.0 58-59 35.69941767985895 39.0 35.0 41.0 29.5 41.0 60-61 35.300364283856084 38.0 35.0 40.0 29.0 41.0 62-63 34.888922719182546 37.0 35.0 40.0 28.0 41.0 64-65 34.75718058790456 37.0 35.0 39.5 28.0 41.0 66-67 34.372121795160325 36.5 35.0 39.0 28.5 41.0 68-69 34.025096907134696 36.0 34.5 39.0 28.0 41.0 70-71 33.591952950546656 35.0 34.0 38.5 27.0 40.5 72-73 33.16113514664046 35.0 34.0 37.0 26.5 39.0 74-75 32.785501502527865 35.0 34.0 37.0 26.0 39.0 76-77 31.3175343742824 34.0 31.5 35.0 24.0 37.0 78-79 32.08179801356586 35.0 33.0 36.0 26.0 37.0 80-81 32.04916474098464 35.0 34.0 35.0 26.0 37.0 82-83 31.908599235118338 35.0 33.5 35.0 25.5 36.0 84-85 31.72146772445035 35.0 33.0 35.0 25.0 36.0 86-87 31.52424696233038 35.0 33.0 35.0 25.0 36.0 88-89 31.36776480766194 35.0 33.0 35.0 24.0 35.5 90-91 31.228224331720313 35.0 33.0 35.0 24.0 35.0 92-93 31.043740153846038 35.0 33.0 35.0 21.5 35.0 94-95 30.986100377558284 35.0 33.0 35.0 20.0 35.0 96-97 30.893014828450912 35.0 33.0 35.0 19.0 35.0 98-99 30.739376910582997 35.0 33.0 35.0 10.0 35.0 100 30.378215901696 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.47092618561762833 1101 2 0.37570527742273185 1101 3 0.38802696922827806 1101 4 0.2915856017479186 1101 5 0.32830611687739264 1101 6 0.36896612538874507 1101 7 0.28492676506976267 1101 8 0.3160234759089704 1101 9 0.3805611928028938 1101 10-11 0.3966674513372581 1101 12-13 0.46448866156172386 1101 14-15 0.6054041034837567 1101 16-17 0.5944205723114706 1101 18-19 0.570391242290107 1101 20-21 0.5727079613155936 1101 22-23 0.54149280415497 1101 24-25 0.591670537560951 1101 26-27 0.6545338065134558 1101 28-29 0.6455707800722976 1101 30-31 0.7163956210810412 1101 32-33 0.70387964064615 1101 34-35 0.6969254667305691 1101 36-37 0.7787747792483586 1101 38-39 0.7666862050216778 1101 40-41 0.760652619260135 1101 42-43 0.8723101841707717 1101 44-45 0.9147616973291832 1101 46-47 0.9212463194935481 1101 48-49 0.9974371792600252 1101 50-51 1.0121905168485696 1101 52-53 1.0218537704503277 1101 54-55 1.1741305474712007 1101 56-57 1.1454693041731119 1101 58-59 1.1213956008289259 1101 60-61 1.1641827210857087 1101 62-63 1.2169914072995667 1101 64-65 1.1618017853759781 1101 66-67 1.1165337508562132 1101 68-69 1.1220961418366358 1101 70-71 1.1612815763456936 1101 72-73 1.1822498386580733 1101 74-75 1.2120796837063992 1101 76-77 1.2534690266681743 1101 78-79 1.272831358172887 1101 80-81 1.2124831627198631 1101 82-83 1.1608044036361385 1101 84-85 1.2006024551100225 1101 86-87 1.2484094272647503 1101 88-89 1.2847700180784614 1101 90-91 1.2844018045744328 1101 92-93 1.3196367862836773 1101 94-95 1.3152414409729438 1101 96-97 1.408227527061225 1101 98-99 1.4118964393619073 1101 100 1.4743408159797404 1102 1 0.4097641506239853 1102 2 0.36566989224635194 1102 3 0.391164342521634 1102 4 0.261878869528978 1102 5 0.2724054979863908 1102 6 0.3219470459339817 1102 7 0.31470380869905057 1102 8 0.33504387769153254 1102 9 0.349503484739742 1102 10-11 0.37929786179311265 1102 12-13 0.3696085584717892 1102 14-15 0.507102226230046 1102 16-17 0.5089927286344178 1102 18-19 0.465421523524995 1102 20-21 0.49073030679094387 1102 22-23 0.48886474936442426 1102 24-25 0.5000522779637677 1102 26-27 0.55479454316621 1102 28-29 0.6648271965711317 1102 30-31 0.6541100018619659 1102 32-33 0.6359625650845118 1102 34-35 0.6708405968066771 1102 36-37 0.7103835408890902 1102 38-39 0.6337799809380371 1102 40-41 0.7193021238712518 1102 42-43 0.7348606095709371 1102 44-45 0.7349230729516663 1102 46-47 0.772538869818554 1102 48-49 0.7771965643632583 1102 50-51 0.7643007017485743 1102 52-53 0.8192670563309221 1102 54-55 0.8983800421863677 1102 56-57 0.9293671306864226 1102 58-59 0.8646633818890166 1102 60-61 0.9977174990597746 1102 62-63 0.9411466754026065 1102 64-65 1.0530630738352684 1102 66-67 1.0654432248257564 1102 68-69 1.013419154463648 1102 70-71 0.9737534437142088 1102 72-73 0.9886364883104122 1102 74-75 0.9883660759565842 1102 76-77 0.980171209979094 1102 78-79 0.9665351345159152 1102 80-81 0.9991892685541259 1102 82-83 0.9943991488890482 1102 84-85 1.0006892776904124 1102 86-87 1.0294671711827768 1102 88-89 1.016701222612241 1102 90-91 1.0101885412106988 1102 92-93 1.0808035003385363 1102 94-95 1.1310742009270633 1102 96-97 1.1729389795744858 1102 98-99 1.1938700602130972 1102 100 1.2598291289038812 1103 1 0.3987917207277718 1103 2 0.3243234080991826 1103 3 0.3325236498171691 1103 4 0.23746439858931012 1103 5 0.2965044792994149 1103 6 0.2571287763960939 1103 7 0.264877574077623 1103 8 0.24770173131746986 1103 9 0.3053729712044557 1103 10-11 0.3132780331210867 1103 12-13 0.3260844660192248 1103 14-15 0.46033690725506915 1103 16-17 0.4583396353093505 1103 18-19 0.4338882968391786 1103 20-21 0.4562969392848615 1103 22-23 0.4619355518926227 1103 24-25 0.494447507484729 1103 26-27 0.5007793408718371 1103 28-29 0.5540754386614637 1103 30-31 0.601894560246798 1103 32-33 0.6268864712498612 1103 34-35 0.569395096526442 1103 36-37 0.6962315728538826 1103 38-39 0.6581704963616346 1103 40-41 0.6432922925676294 1103 42-43 0.6512309153109541 1103 44-45 0.6708555267665659 1103 46-47 0.7580362238305227 1103 48-49 0.7130442280391946 1103 50-51 0.7472072106179795 1103 52-53 0.8238681894552897 1103 54-55 0.898181400569598 1103 56-57 0.9355714586831425 1103 58-59 0.8433384694245873 1103 60-61 0.9348894290206076 1103 62-63 0.9924631239195065 1103 64-65 0.9278772721072528 1103 66-67 0.9225520200323771 1103 68-69 0.8856691750543462 1103 70-71 0.7944479628394419 1103 72-73 0.8438483113201656 1103 74-75 0.9465166431549079 1103 76-77 0.8775419456126521 1103 78-79 0.9506685307485228 1103 80-81 0.8924298288085311 1103 82-83 0.8778430916422231 1103 84-85 0.9581040539750276 1103 86-87 0.9302562802786376 1103 88-89 0.9871824522684634 1103 90-91 1.0620547612880706 1103 92-93 0.9948672464522552 1103 94-95 1.0078950712538948 1103 96-97 1.0500205481903855 1103 98-99 1.0064841544267402 1103 100 1.0772651956324637 1104 1 0.3224035849148805 1104 2 0.1831761995672423 1104 3 0.2254289084343526 1104 4 0.2129712010396858 1104 5 0.2208622193484473 1104 6 0.19971671316447726 1104 7 0.21901528555639516 1104 8 0.19464902636330805 1104 9 0.29301263958544865 1104 10-11 0.28891870610213743 1104 12-13 0.27709960378864906 1104 14-15 0.3944849292293142 1104 16-17 0.41871615233838355 1104 18-19 0.30051183078555255 1104 20-21 0.3539004704372033 1104 22-23 0.3730933991342624 1104 24-25 0.3651697218656125 1104 26-27 0.4248043281941136 1104 28-29 0.4867504038984052 1104 30-31 0.4555147692507404 1104 32-33 0.433374214672412 1104 34-35 0.5249471348330133 1104 36-37 0.532035790298572 1104 38-39 0.4988612887381194 1104 40-41 0.496279907710111 1104 42-43 0.5490263856659965 1104 44-45 0.6050800418931601 1104 46-47 0.5712543728032315 1104 48-49 0.5872141045128245 1104 50-51 0.549755150935404 1104 52-53 0.5545282559300944 1104 54-55 0.4866559353529709 1104 56-57 0.49390817234255735 1104 58-59 0.5621678855470904 1104 60-61 0.6047118615136711 1104 62-63 0.5651975921126606 1104 64-65 0.5254164107528752 1104 66-67 0.5833891671028866 1104 68-69 0.5789172372077829 1104 70-71 0.566960453615188 1104 72-73 0.5274826720081265 1104 74-75 0.4903245269810128 1104 76-77 0.3749095893157808 1104 78-79 0.4774905164855099 1104 80-81 0.499727209418495 1104 82-83 0.5365235526040841 1104 84-85 0.506727038974077 1104 86-87 0.5262993391006354 1104 88-89 0.5438247449206237 1104 90-91 0.54720727510853 1104 92-93 0.5236959376180472 1104 94-95 0.6010881965963897 1104 96-97 0.6657050347659457 1104 98-99 0.6384465341025596 1104 100 0.7077737545700025 1105 1 0.15400525442931468 1105 2 0.09397374802732372 1105 3 0.1413737309931804 1105 4 0.13902196691676494 1105 5 0.13526978813018786 1105 6 0.08239267024796959 1105 7 0.13290416396124272 1105 8 0.09214108829774403 1105 9 0.09918268268204145 1105 10-11 0.1354636728374814 1105 12-13 0.16368892340383212 1105 14-15 0.22379414363984296 1105 16-17 0.19888314691585407 1105 18-19 0.18053405575160042 1105 20-21 0.2384969298631603 1105 22-23 0.271285126939361 1105 24-25 0.2325825675940436 1105 26-27 0.262591542206728 1105 28-29 0.2913717087012557 1105 30-31 0.19922807473726323 1105 32-33 0.22157662361718167 1105 34-35 0.3249240849228059 1105 36-37 0.319225755526908 1105 38-39 0.31011668120395797 1105 40-41 0.303412924832827 1105 42-43 0.3626161813763886 1105 44-45 0.3563590018812093 1105 46-47 0.34338972726772 1105 48-49 0.3431705248159389 1105 50-51 0.3097578953574569 1105 52-53 0.3084705993430248 1105 54-55 0.39366380898662356 1105 56-57 0.4580526755994683 1105 58-59 0.4196387793066876 1105 60-61 0.4261312668960784 1105 62-63 0.3179427690200498 1105 64-65 0.2586933639160023 1105 66-67 0.30192156449304974 1105 68-69 0.25824262699389067 1105 70-71 0.27700253115884976 1105 72-73 0.22479958158400137 1105 74-75 0.3161901926435533 1105 76-77 0.24587729733515573 1105 78-79 0.2746461036489478 1105 80-81 0.28378656549836023 1105 82-83 0.3123162403638027 1105 84-85 0.3177036488418068 1105 86-87 0.2761406047482957 1105 88-89 0.30830593862020805 1105 90-91 0.34296282959770963 1105 92-93 0.35785207636179095 1105 94-95 0.4152163088060483 1105 96-97 0.4278750368849167 1105 98-99 0.35908086763125624 1105 100 0.40661322529014754 1106 1 0.17778208285453445 1106 2 0.0378591317502881 1106 3 0.1126355807937287 1106 4 0.08442373773188905 1106 5 0.11872640375563748 1106 6 0.07409135355931795 1106 7 0.0744347847199478 1106 8 0.09000901814851403 1106 9 0.12254794657601309 1106 10-11 0.14096511408312296 1106 12-13 0.13368784569774306 1106 14-15 0.20395205986284992 1106 16-17 0.1564658025028436 1106 18-19 0.1117801571374244 1106 20-21 0.12177557499292391 1106 22-23 0.18535438122634673 1106 24-25 0.18122443443923686 1106 26-27 0.1893790940558091 1106 28-29 0.1535175556093762 1106 30-31 0.12838653030995317 1106 32-33 0.10767766675721191 1106 34-35 0.10444190829423405 1106 36-37 0.133044877707988 1106 38-39 0.11430600287489057 1106 40-41 0.18671687370684964 1106 42-43 0.2587614948945074 1106 44-45 0.1945312272598514 1106 46-47 0.15518671028664954 1106 48-49 0.16901572947083565 1106 50-51 0.1430644267529786 1106 52-53 0.24928640795037182 1106 54-55 0.25741310375959614 1106 56-57 0.27694809142516164 1106 58-59 0.2575668570763412 1106 60-61 0.23045559981090946 1106 62-63 0.13498110357180337 1106 64-65 0.2155188192344113 1106 66-67 0.25790027557643214 1106 68-69 0.21391831394461036 1106 70-71 0.24327234207854787 1106 72-73 0.23249999021836487 1106 74-75 0.25147041609125864 1106 76-77 0.07822779913894706 1106 78-79 0.19179193565791053 1106 80-81 0.2105407183915844 1106 82-83 0.22672082513407688 1106 84-85 0.21964764189680963 1106 86-87 0.2720717179739829 1106 88-89 0.2402504879106111 1106 90-91 0.30241960116772404 1106 92-93 0.3629406618154398 1106 94-95 0.4044692697662704 1106 96-97 0.44835450683231315 1106 98-99 0.4037887347863496 1106 100 0.47168169190807774 1107 1 0.17152175670916847 1107 2 0.1307265135809601 1107 3 0.13716202083453766 1107 4 0.11401008360657272 1107 5 0.12247591086313037 1107 6 0.1324430623542625 1107 7 0.06935548484300824 1107 8 0.10817466144040822 1107 9 0.09913517398091187 1107 10-11 0.17802366545596016 1107 12-13 0.1982762884942133 1107 14-15 0.22817863725132526 1107 16-17 0.19297644850801277 1107 18-19 0.18798198942984357 1107 20-21 0.22186325262343587 1107 22-23 0.18308379998403268 1107 24-25 0.19412595498897645 1107 26-27 0.223753498043763 1107 28-29 0.21463303379646703 1107 30-31 0.2680212752016544 1107 32-33 0.26449164316422014 1107 34-35 0.2935113121005415 1107 36-37 0.31687100736842666 1107 38-39 0.29583399756111106 1107 40-41 0.27461982988879896 1107 42-43 0.38044815272000676 1107 44-45 0.2586812227755715 1107 46-47 0.38687070403288004 1107 48-49 0.3456240940175448 1107 50-51 0.3347197423858219 1107 52-53 0.35485833071174966 1107 54-55 0.4041029994442482 1107 56-57 0.3916632411812273 1107 58-59 0.43591720876128903 1107 60-61 0.38785424748794384 1107 62-63 0.4071522080370116 1107 64-65 0.35673245209249416 1107 66-67 0.36571988514879905 1107 68-69 0.2782905013353414 1107 70-71 0.2612596666050351 1107 72-73 0.26145800457487667 1107 74-75 0.24695992930644906 1107 76-77 0.20954469912699025 1107 78-79 0.31482229320081245 1107 80-81 0.30520248675367156 1107 82-83 0.30684332878961484 1107 84-85 0.2852104579724113 1107 86-87 0.3275499273002289 1107 88-89 0.35460322922548215 1107 90-91 0.35048133921834435 1107 92-93 0.3506350114130292 1107 94-95 0.3858082387562334 1107 96-97 0.4400333625762052 1107 98-99 0.4595692428459479 1107 100 0.5539856824642193 1108 1 0.15884796035606286 1108 2 0.0858141574622735 1108 3 0.04756546195390854 1108 4 0.09399673363796524 1108 5 0.1323082124693329 1108 6 0.07966098216557782 1108 7 0.09742838392763531 1108 8 0.08451365064428984 1108 9 0.12514717705452938 1108 10-11 0.1524960227853498 1108 12-13 0.1312560128859488 1108 14-15 0.1109103549933792 1108 16-17 0.1283913262458114 1108 18-19 0.15761918948953024 1108 20-21 0.17423703412431735 1108 22-23 0.1259307369877547 1108 24-25 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-0.873538605746873 2316 72-73 -0.8242013334937823 2316 74-75 -0.8266132183736588 2316 76-77 -0.8514370652947569 2316 78-79 -0.914792862299521 2316 80-81 -0.9026961946271577 2316 82-83 -0.913199223905206 2316 84-85 -0.8881248238511006 2316 86-87 -0.9105988135568062 2316 88-89 -0.8123654775410749 2316 90-91 -0.846107445921163 2316 92-93 -0.9210215597154594 2316 94-95 -0.9674949261943802 2316 96-97 -0.9818385614982184 2316 98-99 -0.9819313229503983 2316 100 -0.9866878871225921 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24.0 3 32.0 4 64.0 5 143.0 6 317.0 7 750.0 8 1699.0 9 2928.0 10 5169.0 11 9565.0 12 16470.0 13 22827.0 14 25227.0 15 30800.0 16 39657.0 17 51145.0 18 50835.0 19 33859.0 20 25228.0 21 22735.0 22 24498.0 23 27873.0 24 30891.0 25 33456.0 26 40392.0 27 50385.0 28 63121.0 29 79462.0 30 99040.0 31 123295.0 32 155688.0 33 204807.0 34 290619.0 35 473843.0 36 907262.0 37 1833996.0 38 1525300.0 39 250290.0 40 263.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.36364683614703 17.97609229846711 13.595699085514015 23.06456177987185 2 13.506871346212243 23.494258903329694 35.42554437248555 27.57332537797252 3 20.459924593818794 29.169637657182694 29.14830146844259 21.222136280555926 4 13.826114515654773 19.571825545964238 33.28450444091147 33.31755549746952 5 12.846227964641196 38.02240021483211 32.586415378195305 16.544956442331387 6 30.855338494084872 31.900188512127407 18.805713496659653 18.43875949712807 7 23.967833163334202 33.51625087447198 22.503068757719575 20.012847204474244 8 28.978502293653218 30.46711489474676 22.04446017709917 18.509922634500846 9 29.33239547723474 14.909882658638942 19.55602380547318 36.20169805865313 10-11 24.921720888535717 27.049444903582216 24.42563848694944 23.603195720932632 12-13 28.032663395556444 24.68071124218066 25.22774842987625 22.058876932386646 14-15 27.659341215168514 22.8164381663797 24.73138065775949 24.79283996069229 16-17 23.705028100713985 26.15434626512414 28.669403652101046 21.47122198206083 18-19 26.016023912278925 25.295390340641642 29.013961798639144 19.674623948440292 20-21 24.383223367373024 25.518119683928507 28.406556195399276 21.69210075329919 22-23 22.48053830221425 27.62716517014509 24.045599378177812 25.846697149462848 24-25 23.17533350320648 28.664697867379928 23.177843455280893 24.982125174132694 26-27 24.82561202675467 25.17656987321025 24.25748647260167 25.74033162743341 28-29 23.916356107723637 24.00944177514772 28.45001949679724 23.624182620331403 30-31 26.787750997244807 24.54320061606941 26.696254558609983 21.972793828075805 32-33 20.95377518463705 26.459208118438088 28.302759674186472 24.28425702273839 34-35 20.56388657451681 27.44106959363678 28.450788532273418 23.544255299572992 36-37 24.37746711404685 27.21315102438161 25.789560416924157 22.619821444647386 38-39 25.61202488588274 24.679128528716443 25.483158267448548 24.225688317952272 40-41 23.23709686352733 26.952431769158196 25.481542186160862 24.328929181153608 42-43 27.00853781440801 25.127815453207575 25.234868095306755 22.62877863707766 44-45 26.70575768603137 24.14429651804603 26.654994289690087 22.494951506232518 46-47 23.986232766163013 25.005103892049558 29.543867685146164 21.464795656641268 48-49 25.037389642954466 26.295950505603184 28.247573907293216 20.419085944149135 50-51 22.687574937052112 27.53235739568625 26.24966546299464 23.530402204267 52-53 21.815208823081516 28.48734530874773 23.31353690174681 26.383908966423935 54-55 23.406212674669387 27.264996628273696 23.023252016664674 26.305538680392242 56-57 24.981113571306913 24.39841192224316 23.77222188646349 26.848252619986436 58-59 24.284188406771438 24.281174149947145 27.346551243777757 24.088086199503664 60-61 24.911336477267522 25.391648734032323 25.781969690032508 23.91504509866764 62-63 20.488984150088424 26.60700439394953 27.41024623161854 25.493765224343505 64-65 20.49878813291902 26.839529389073725 26.7454960110011 25.916186467006145 66-67 24.051479247146222 26.328902513937415 24.550041791642343 25.069576447274024 68-69 24.967402795253697 24.67429306349945 24.58938126458158 25.76892287666527 70-71 22.847131871607672 25.958770163243717 25.18795174141734 26.006146223731267 72-73 25.25081241678621 24.612330181317937 25.303643539375475 24.833213862520374 74-75 25.565643759139007 23.623844706734687 26.145832872393488 24.664678661732818 76-77 22.864109067589112 25.65650795307391 27.9803066305293 23.499076348807677 78-79 23.415843075982938 25.40732978860971 27.597195398598313 23.57963173680904 80-81 21.664628457168227 26.04868358113536 26.682804505066027 25.603883456630385 82-83 21.508391800719192 27.33807938595036 23.835094316447503 27.318434496882947 84-85 22.22922223744628 26.553298970362015 23.86178633539387 27.355692456797836 86-87 22.6855034498848 24.019179682615636 25.05844407948868 28.236872788010892 88-89 22.786482998391733 23.329849685384556 27.044058216159144 26.839609100064564 90-91 23.633240589784883 24.31474927581453 26.13373075482364 25.91827937957694 92-93 20.577875504108007 25.450857298315217 27.053162062361924 26.918105135214848 94-95 19.722526284566342 26.009521737312717 27.425018836268052 26.842933141852892 96-97 22.20864770496313 25.237974918015055 25.514235925313404 27.039141451708414 98-99 22.80125512000006 24.115934576908142 25.40950464261656 27.67330566047524 100 22.489526853002424 25.273195945500575 25.912356970745986 26.324920230751015 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 873.0 1 521.0 2 217.5 3 258.0 4 225.0 5 291.0 6 495.5 7 939.5 8 1387.5 9 1388.0 10 1155.5 11 1067.0 12 1219.0 13 1418.5 14 1627.0 15 2029.0 16 2414.0 17 2821.0 18 3335.0 19 3863.0 20 4472.0 21 5301.5 22 6283.5 23 7576.0 24 9185.5 25 11214.0 26 14524.5 27 18441.0 28 22591.5 29 29314.0 30 37470.0 31 44977.5 32 54169.0 33 64321.5 34 74721.0 35 82849.5 36 89154.5 37 97154.5 38 99476.0 39 97969.5 40 95104.0 41 91899.5 42 92704.5 43 102245.5 44 119132.0 45 135038.5 46 163418.0 47 213425.0 48 394645.5 49 609422.5 50 849190.5 51 796498.5 52 423281.5 53 248271.5 54 193184.0 55 158953.5 56 142639.5 57 126865.0 58 120987.5 59 114556.5 60 95596.0 61 75393.5 62 61253.5 63 47196.5 64 33286.5 65 28345.0 66 22944.5 67 16122.5 68 12076.5 69 10169.0 70 9281.5 71 8462.0 72 7880.5 73 9511.5 74 7741.5 75 5569.0 76 4215.5 77 2683.0 78 1928.5 79 1153.5 80 827.5 81 699.0 82 546.0 83 500.5 84 394.5 85 207.5 86 121.5 87 51.0 88 25.0 89 15.5 90 6.5 91 4.5 92 3.5 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.06216093946327065 3 0.02604534208733505 4 0.00698814685178644 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.340286895469987E-5 12-13 1.983535132603138E-4 14-15 3.738200826828991E-4 16-17 4.4248091419608464E-4 18-19 0.0 20-21 0.0028150940920406075 22-23 0.004775742280806018 24-25 6.866083151318555E-4 26-27 5.18770726988513E-4 28-29 0.004798629224643746 30-31 0.00225817845865588 32-33 0.0012740398736335542 34-35 0.0015486831996862963 36-37 0.0037382008268289912 38-39 0.004264600535096747 40-41 9.765096037430833E-4 42-43 0.0025938536349425652 44-45 3.0515925116971355E-4 46-47 0.002029309020278595 48-49 1.5257962558485676E-5 50-51 0.001846213469576767 52-53 0.006362570386888527 54-55 0.014220421104508652 56-57 0.02488573693289014 58-59 0.02684638512165555 60-61 0.025251928034293797 62-63 0.007804447848665424 64-65 0.006576181862707327 66-67 1.983535132603138E-4 68-69 0.0014571354243353822 70-71 1.8309555070182814E-4 72-73 8.086720155997409E-4 74-75 0.004821516168481474 76-77 0.00112908922932794 78-79 0.0012587819110750685 80-81 0.0 82-83 0.0013350717238674967 84-85 1.5257962558485676E-5 86-87 6.179474836186699E-4 88-89 2.2886943837728518E-5 90-91 6.103185023394271E-4 92-93 7.934140530412552E-4 94-95 0.001663117918874939 96-97 1.2206370046788541E-4 98-99 0.0 100 7.781560904827695E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6553955.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.467364421364696 #Duplication Level Percentage of deduplicated Percentage of total 1 79.977476238961 19.56838056639695 2 11.45715212720618 5.606526326547347 3 3.149787740859792 2.312010135166907 4 1.3485127728066526 1.3197821375650136 5 0.744976785598819 0.9113809249351589 6 0.48551304557397323 0.712753477043103 7 0.3536029487327408 0.6056212544979175 8 0.2583868377446366 0.5057635936625644 9 0.20543623732316188 0.4523834955545789 >10 1.5100054439785355 7.48596878045525 >50 0.2198584086303319 3.7804518033610366 >100 0.22766477323730339 11.817084926862776 >500 0.036384267156044287 6.140857547563283 >1k 0.02058417881913297 9.181291210444911 >5k 0.0015107654179180162 2.5367776575491003 >10k+ 0.003147427953995867 27.062966162394236 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 192507 2.937264598246402 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 156940 2.3945846439287424 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 92255 1.4076233358330963 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 81542 1.2441647829440392 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 79533 1.2135115361640414 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 78191 1.1930353504105535 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 75495 1.1518998833528762 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 72437 1.105241033849027 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 61212 0.9339704041300254 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 60418 0.9218555818585876 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 53578 0.8174911179585456 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 51187 0.7810093294812064 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 34913 0.5327012468044104 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 33774 0.5153224274502953 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 31674 0.48328070607747536 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 30654 0.46771758426781995 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 29325 0.44743975202759256 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 27363 0.4175036294878436 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 26847 0.40963052080766504 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 23869 0.36419230830849464 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 22678 0.3460200749013382 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 21978 0.33533950111039823 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 21552 0.3288396090604833 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 21515 0.32827506444581933 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 20591 0.3141767070417786 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 19960 0.3045489326673741 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 18999 0.28988603064866936 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 18986 0.2896876771354091 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 16312 0.24888788525401837 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 16074 0.2452564901650988 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 16026 0.24452410796229146 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 15952 0.24339501873296351 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 15925 0.24298305374388443 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 15919 0.2428915059685335 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 15441 0.23559819986557737 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 15031 0.22934243521659822 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 14468 0.2207522022961708 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14162 0.21608326575327413 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 13758 0.20991904887964594 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 13527 0.20639445952863578 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 12498 0.190694016055954 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 12389 0.18903089813707907 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 11848 0.18077634039293833 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 11746 0.17922002821197275 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 11695 0.17844187212148999 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 11498 0.17543605349746833 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 11427 0.17435273815581584 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 10565 0.1612003744304012 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10486 0.1599949953882808 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA 10404 0.158743842458485 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 9342 0.1425398862213732 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9302 0.14192956771903378 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 8542 0.13033351617458466 No Hit GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA 8513 0.12989103526038856 No Hit ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT 8163 0.12455074836491858 No Hit GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG 8097 0.12354372283605852 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT 7662 0.11690650912311726 No Hit TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 7127 0.10874349915432743 No Hit TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC 7003 0.1068515117970752 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 6921 0.10560035886727936 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA 6892 0.10515787795308329 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 6860 0.10466962315121175 No Hit TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA 6829 0.1041966263118987 No Hit GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC 6622 0.10103822806229217 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.004546872842428732 0.0 0.0 3.0515925116971356E-5 0.0 2 0.00674401945085067 0.0 0.0 3.0515925116971356E-5 0.0 3 0.007079694627137354 0.0 0.0 3.0515925116971356E-5 0.0 4 0.008285073669257723 0.0 0.0 4.5773887675457035E-5 0.0 5 0.031645014346299294 0.0 0.0 2.4412740093577085E-4 0.0 6 0.0720175832760524 0.0 0.0 5.6454461466397E-4 0.0 7 0.08214887041488689 0.0 0.0 7.781560904827696E-4 0.0 8 0.09702538390941043 0.0 0.0 8.391879407167122E-4 0.0 9 0.1067447060591658 0.0 0.0 9.00219790950655E-4 0.0 10-11 0.2090188290886953 0.0 0.0 0.0018156975444597956 7.628981279242839E-6 12-13 0.3550756756797995 0.0 0.0 0.0036313950889195912 3.0515925116971356E-5 14-15 0.5220511889385875 0.0 0.0 0.005813283734783043 3.8144906396214194E-5 16-17 0.5941526910087116 0.0 0.0 0.007049178702020383 6.103185023394271E-5 18-19 0.6251263550024375 0.0 0.0 0.007796818867386181 6.103185023394271E-5 20-21 0.7224492691817384 0.0 0.0 0.009902417700457206 6.866083151318555E-5 22-23 0.7498907148431748 0.0 0.0 0.0124657554102828 7.628981279242839E-5 24-25 0.8699556222158986 0.0 0.0 0.021803628496076033 1.0680573790939975E-4 26-27 1.1050045354293705 0.0 0.0 0.05247213323863224 1.2206370046788542E-4 28-29 1.1673333124807845 0.0 0.0 0.08717636907790792 1.2206370046788542E-4 30-31 1.1952553839628133 0.0 0.0 0.15573039485318407 1.2206370046788542E-4 32-33 1.2211557754058426 0.0 0.0 0.2746585840153007 1.2206370046788542E-4 34-35 1.2423567143808585 0.0 0.0 0.43387542331309875 1.2206370046788542E-4 36-37 1.2773966253964208 0.0 0.0 0.6580225222785325 1.2206370046788542E-4 38-39 1.3129553071389717 0.0 0.0 1.0076892502313488 1.2206370046788542E-4 40-41 1.3480638789860473 0.0 0.0 1.5553890742307508 1.2206370046788542E-4 42-43 1.420058575318262 0.0 0.0 1.9277825374144315 1.2206370046788542E-4 44-45 1.5010936144663796 0.0 0.0 2.3043337953952996 1.2206370046788542E-4 46-47 1.528603720959329 0.0 0.0 2.7311524110250986 1.2206370046788542E-4 48-49 1.546943791954629 0.0 0.0 3.0956651365473213 1.373216630263711E-4 50-51 1.5831509371059154 0.0 0.0 3.5152362199618397 1.373216630263711E-4 52-53 1.594228217923376 0.0 0.0 4.07030563987699 1.6020860686409962E-4 54-55 1.6338302597439256 0.0 0.0 4.537870644519225 1.8309555070182814E-4 56-57 1.7074880739950151 0.0 0.0 5.014788780209813 1.8309555070182814E-4 58-59 1.7321525704708072 0.0 0.0 5.514059831048581 1.8309555070182814E-4 60-61 1.7531246400074458 0.0 0.0 5.933524108725189 1.8309555070182814E-4 62-63 1.77122058360181 0.0 0.0 6.369764516234854 1.8309555070182814E-4 64-65 1.7855859553506241 0.0 0.0 6.8617956638396205 1.8309555070182814E-4 66-67 1.8066037987749382 0.0 0.0 7.365560184651863 1.9835351326031382E-4 68-69 1.8297959018638363 0.0 0.0 8.014199059956926 2.2124045709804232E-4 70-71 1.859876975047891 0.0 0.0 8.844277997026223 2.2886943837728516E-4 72-73 1.9304908257685627 0.0 0.0 9.473058634061417 2.2886943837728516E-4 74-75 2.000936838901091 7.628981279242839E-6 0.0 10.081088442017073 2.2886943837728516E-4 76-77 2.0235567683940463 1.5257962558485678E-5 0.0 10.729857010003883 2.2886943837728516E-4 78-79 2.0401574316576783 1.5257962558485678E-5 0.0 11.315160082728674 2.2886943837728516E-4 80-81 2.0699180876280048 1.5257962558485678E-5 0.0 11.999273720982217 2.36498419656528E-4 82-83 2.081033513351861 1.5257962558485678E-5 0.0 12.869954096419644 2.4412740093577085E-4 84-85 2.120666071097528 1.5257962558485678E-5 0.0 13.644455904869655 2.4412740093577085E-4 86-87 2.1871221270210124 1.5257962558485678E-5 0.0 14.390036245290059 2.5175638221501366E-4 88 2.2045162043376862 1.5257962558485678E-5 0.0 14.997707491125588 2.593853634942565E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 2675 0.0 38.308243 3 CGATCTC 2500 0.0 35.33715 5 CCGATCT 3340 0.0 30.954445 4 TATCACG 295 0.0 30.295135 2 GATCTAC 2795 0.0 28.945957 1 TCTCGCG 1380 0.0 28.943665 8 CTCGCGC 1605 0.0 25.471699 9 TCTACAC 3900 0.0 23.623865 3 AGTACTC 3105 0.0 23.457611 5 ATCTACA 3590 0.0 23.322058 2 TACACTC 3930 0.0 22.359535 5 CGATCTG 715 0.0 21.688103 5 TCACGCA 445 0.0 21.121113 4 ATCACGC 475 0.0 20.781898 3 ATCTCGC 3250 0.0 20.386818 7 CGTTAAC 440 0.0 20.31151 1 TATTCGC 315 3.7289283E-10 19.393066 9 AAGTACT 4005 0.0 19.243681 4 CGCGCCA 1830 0.0 18.506432 1 AAAGTAC 3995 0.0 18.473207 3 >>END_MODULE