FastQCFastQC Report
Fri 27 May 2016
ERR765334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765334_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14390310
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT370980.25779847689174173No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA355780.24723581354397506No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196430.13650157640801344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT59850.018.8463064
GTACAAG70400.018.7633111
GTACTAG48200.018.3352411
GTATAAG63200.018.0000671
GTACATG1143150.017.937351
TACATGG1140350.017.488472
CATGGGG335000.017.3962674
GTACATA35600.017.0339571
TCTAACG24300.017.0213242
CTAGTAC68100.016.4255943
ACATGGG1072950.016.1723633
GTATCAA846350.016.118451
TAGGCAT72800.015.9443575
ATTTAGG64900.015.4280391
ATAAGGT54550.015.4223153
GTATAAT42850.015.1392411
GTATAAA185700.015.0872541
CTAGGCA78800.014.9701694
CTAACGC27450.014.8959483
GTACAAA71100.014.8760881
Produced by FastQC (version 0.11.5)