FastQCFastQC Report
Fri 27 May 2016
ERR765329_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765329_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7346311
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA210830.2869875778468949No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT195720.2664194314670315No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG79390.10806784520829571No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT34300.023.9951064
GTACTAG27150.022.6084751
GTATAAG31150.022.2431031
GTGATCG24150.020.4094948
CTAGTAC41050.020.3893263
TAGGCAT40450.019.6571755
GTACAAG36150.019.5525071
GTACATA19550.019.5044751
TGATCGC25050.019.4905959
ACGTTAA7800.019.0765088
CTAGGCA44750.018.9113224
CTGTGCG43750.018.0681789
ATAAGGT29850.017.9141853
TAAGGTG30250.017.6773014
CGTTAAC9550.017.5293671
CTATTGA32400.017.3653629
CATGGGG178000.017.2934444
GTACATG590300.016.8580821
GTACTGT50000.016.8326346
GTATAGA16650.016.7573051
Produced by FastQC (version 0.11.5)