##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765329_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7346311 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39057371788371 33.0 33.0 33.0 27.0 33.0 2 31.285967746260674 33.0 33.0 33.0 27.0 33.0 3 31.328826263957517 33.0 33.0 33.0 27.0 33.0 4 34.94502506087749 37.0 37.0 37.0 33.0 37.0 5 35.084700879121506 37.0 37.0 37.0 33.0 37.0 6 35.00993437386465 37.0 37.0 37.0 33.0 37.0 7 34.88272100105754 37.0 37.0 37.0 33.0 37.0 8 34.80796987767057 37.0 37.0 37.0 33.0 37.0 9 34.750538467538334 37.0 37.0 37.0 33.0 37.0 10-11 34.734569228011175 37.0 37.0 37.0 30.0 37.0 12-13 34.67831105979586 37.0 37.0 37.0 27.0 37.0 14-15 36.136210949958425 37.0 37.0 40.0 27.0 40.0 16-17 36.19703344984987 37.0 37.0 40.0 27.0 40.0 18-19 36.21888523369076 37.0 37.0 40.0 27.0 40.0 20-21 36.236971930537656 37.0 37.0 40.0 27.0 40.0 22-23 36.29245072799123 37.0 37.0 40.0 30.0 40.0 24-25 36.257731057125135 37.0 37.0 40.0 27.0 40.0 26-27 36.2698874305757 37.0 37.0 40.0 30.0 40.0 28-29 36.20219120862158 37.0 37.0 40.0 27.0 40.0 30-31 36.165002611514815 37.0 37.0 40.0 27.0 40.0 32-33 36.034391138627264 37.0 37.0 40.0 27.0 40.0 34-35 35.98044045508011 37.0 37.0 40.0 27.0 40.0 36-37 35.8628336725739 37.0 37.0 40.0 27.0 40.0 38-39 35.65459616397945 37.0 37.0 40.0 27.0 40.0 40-41 35.5726616392908 37.0 35.0 40.0 27.0 40.0 42-43 35.43923684962425 37.0 33.0 40.0 27.0 40.0 44-45 35.29789944912487 37.0 33.0 40.0 27.0 40.0 46-47 35.17542117397426 37.0 33.0 40.0 27.0 40.0 48-49 35.011652447058125 37.0 33.0 40.0 27.0 40.0 50-51 34.84294695664259 37.0 33.0 40.0 27.0 40.0 52-53 34.65308166234726 37.0 33.0 40.0 27.0 40.0 54-55 34.52407453210189 37.0 33.0 40.0 27.0 40.0 56-57 34.32830715987929 37.0 33.0 40.0 27.0 40.0 58-59 34.09527026557956 37.0 33.0 37.0 27.0 40.0 60-61 33.86132134890559 37.0 33.0 37.0 27.0 40.0 62-63 33.678151455880375 37.0 33.0 37.0 27.0 40.0 64-65 33.37435775588591 37.0 33.0 37.0 22.0 40.0 66-67 33.159662312145514 37.0 33.0 37.0 22.0 40.0 68-69 32.865576886140545 37.0 33.0 37.0 22.0 38.5 70-71 32.73273926192343 37.0 33.0 37.0 22.0 37.0 72-73 32.472157045896914 33.0 33.0 37.0 22.0 37.0 74-75 32.19871320449134 33.0 33.0 37.0 22.0 37.0 76-77 32.01620180523258 33.0 33.0 37.0 22.0 37.0 78-79 31.786316424665387 33.0 33.0 37.0 22.0 37.0 80-81 31.5412292645928 33.0 33.0 37.0 22.0 37.0 82-83 31.36985570036444 33.0 27.0 37.0 22.0 37.0 84-85 31.194455080924293 33.0 27.0 37.0 22.0 37.0 86-87 30.994047760842143 33.0 27.0 37.0 18.5 37.0 88-89 30.824643552389766 33.0 27.0 37.0 15.0 37.0 90-91 30.63271980998354 33.0 27.0 37.0 15.0 37.0 92-93 30.485346386778343 33.0 27.0 37.0 15.0 37.0 94-95 30.25217643249789 33.0 27.0 37.0 15.0 37.0 96-97 30.17330916156422 33.0 27.0 37.0 15.0 37.0 98-99 29.00944685026267 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3293900254751563 1101 2 0.33362182306412436 1101 3 0.3822490843351538 1101 4 0.4615201887163636 1101 5 0.380372512410851 1101 6 0.36537859965801545 1101 7 0.2650912442345472 1101 8 0.35755212745295495 1101 9 0.30974840291133887 1101 10-11 0.35005215516557797 1101 12-13 0.3674611478674876 1101 14-15 0.4995659336710432 1101 16-17 0.4433618156381627 1101 18-19 0.3723186419550899 1101 20-21 0.46188483903104327 1101 22-23 0.3554434053727533 1101 24-25 0.4610841895344464 1101 26-27 0.42415507851953294 1101 28-29 0.3268662583835962 1101 30-31 0.34242245809461735 1101 32-33 0.4020103426064807 1101 34-35 0.4046856425027414 1101 36-37 0.4466267987344281 1101 38-39 0.36083814687425075 1101 40-41 0.36760364487418684 1101 42-43 0.3848693609819094 1101 44-45 0.39596601618544014 1101 46-47 0.3734495517350851 1101 48-49 0.39749981916446586 1101 50-51 0.43803918286999277 1101 52-53 0.342381858095834 1101 54-55 0.4326517747035794 1101 56-57 0.4560125788105083 1101 58-59 0.39103823569584506 1101 60-61 0.48042238683486005 1101 62-63 0.47421668386946436 1101 64-65 0.31398950744257803 1101 66-67 0.3610411714364261 1101 68-69 0.3825900182360442 1101 70-71 0.38035795288128327 1101 72-73 0.4533461504435934 1101 74-75 0.43180439193770326 1101 76-77 0.3481378456200588 1101 78-79 0.39938654778626415 1101 80-81 0.3703007494642776 1101 82-83 0.3576187777036246 1101 84-85 0.3054220429475869 1101 86-87 0.3348928020153181 1101 88-89 0.38060792584869674 1101 90-91 0.30922563736940134 1101 92-93 0.41888468371859844 1101 94-95 0.3843054441220133 1101 96-97 0.35559017308995067 1101 98-99 0.3220404474538796 1102 1 0.1823760413772817 1102 2 0.2608408233997892 1102 3 0.2484716494438608 1102 4 0.3465935698499223 1102 5 0.2956212250078778 1102 6 0.25298784674689045 1102 7 0.1753423161365859 1102 8 0.25493787388295175 1102 9 0.24240585200674758 1102 10-11 0.2033574665672191 1102 12-13 0.24727932723187251 1102 14-15 0.26949470630870564 1102 16-17 0.2593735414645124 1102 18-19 0.2898923874158541 1102 20-21 0.31285498945825907 1102 22-23 0.23652702902255385 1102 24-25 0.3404635790272863 1102 26-27 0.31304464013949485 1102 28-29 0.15010917047048622 1102 30-31 0.2557901155703348 1102 32-33 0.3483026307187842 1102 34-35 0.2665988203262373 1102 36-37 0.3904141995380641 1102 38-39 0.3929016528522524 1102 40-41 0.3500542415474399 1102 42-43 0.33168859562609754 1102 44-45 0.24573875896325603 1102 46-47 0.27325358888916185 1102 48-49 0.2890742587351198 1102 50-51 0.2660217288417783 1102 52-53 0.25657089544367295 1102 54-55 0.31061888706901897 1102 56-57 0.29056768669036614 1102 58-59 0.2926734922187251 1102 60-61 0.3248725474297913 1102 62-63 0.34696664297938185 1102 64-65 0.2521667521036832 1102 66-67 0.21166388309320183 1102 68-69 0.2651123536297959 1102 70-71 0.32804140757240674 1102 72-73 0.2401124985205101 1102 74-75 0.24094440514387117 1102 76-77 0.2817589262548097 1102 78-79 0.2947568744772653 1102 80-81 0.2835092173077136 1102 82-83 0.19609800845256942 1102 84-85 0.0672713443370121 1102 86-87 0.23746763271924465 1102 88-89 0.3282821934271887 1102 90-91 0.28003040491130804 1102 92-93 0.36153395975059865 1102 94-95 0.33046986793218736 1102 96-97 0.2835388431315806 1102 98-99 0.16746018273379093 1103 1 0.025843670262016616 1103 2 0.11483587463195022 1103 3 0.11691781791099842 1103 4 0.13698558663133298 1103 5 0.10832713008289829 1103 6 0.0986772805510654 1103 7 -0.005075639634888773 1103 8 -0.026788642355249692 1103 9 0.08909525572943977 1103 10-11 0.04135573562552253 1103 12-13 0.05588359453668801 1103 14-15 0.06230653872390235 1103 16-17 0.016979817255219132 1103 18-19 0.05099407002448686 1103 20-21 -0.015208656947038435 1103 22-23 0.04847932823255263 1103 24-25 0.12453872482718253 1103 26-27 0.20241219613948402 1103 28-29 0.1282872156788173 1103 30-31 0.15547933985823192 1103 32-33 0.18027058121586492 1103 34-35 0.11745078289126099 1103 36-37 0.11038506510053736 1103 38-39 0.12576853680285893 1103 40-41 0.07136584097307974 1103 42-43 0.08820819630300036 1103 44-45 0.011925905804886838 1103 46-47 0.005196030942379082 1103 48-49 0.06771455327088916 1103 50-51 0.07034549521134892 1103 52-53 0.0023100001718887597 1103 54-55 0.06604876799745085 1103 56-57 0.036903345216465766 1103 58-59 0.03360391878947411 1103 60-61 0.13560827387030372 1103 62-63 0.1119005679205074 1103 64-65 0.04387945518710268 1103 66-67 0.065541713863567 1103 68-69 0.062053424178486694 1103 70-71 0.017030776162208383 1103 72-73 0.06778765197455527 1103 74-75 0.08593715680522251 1103 76-77 -0.003582560537196855 1103 78-79 0.040530572904053486 1103 80-81 0.04201272788094457 1103 82-83 0.04799424827604781 1103 84-85 0.06407358603138036 1103 86-87 0.10571332962881996 1103 88-89 0.16571047119938598 1103 90-91 0.06631117568793954 1103 92-93 0.100655808462502 1103 94-95 -0.009063213219448585 1103 96-97 -0.07667850891842676 1103 98-99 0.0417571538858148 1104 1 0.2054155148863508 1104 2 0.09814705454465056 1104 3 0.10979364802754432 1104 4 0.1589913980048152 1104 5 0.1928818323769903 1104 6 0.11374585141314952 1104 7 0.051186313450493515 1104 8 0.07510237624727978 1104 9 0.08582919096031816 1104 10-11 0.11679031478208657 1104 12-13 0.06969646332698431 1104 14-15 0.14347962492394117 1104 16-17 0.22326138794797146 1104 18-19 0.15195782290570747 1104 20-21 0.13536186129473293 1104 22-23 0.22182789531744618 1104 24-25 0.08907638739697177 1104 26-27 0.1765389234496979 1104 28-29 0.12068430187768087 1104 30-31 0.1319937926231063 1104 32-33 0.14922884441147488 1104 34-35 0.14301039773077662 1104 36-37 0.1848853258980796 1104 38-39 0.11799028445119575 1104 40-41 0.14061696829605808 1104 42-43 0.10639932150377973 1104 44-45 0.06602905418834837 1104 46-47 0.044826185266749974 1104 48-49 0.04313117517081366 1104 50-51 0.12478431102812948 1104 52-53 0.07478670955567424 1104 54-55 0.17956804103540236 1104 56-57 0.17414907045416328 1104 58-59 0.15063324379502774 1104 60-61 0.13763822903045053 1104 62-63 0.05868782823038998 1104 64-65 0.05764265540107516 1104 66-67 0.06654957188899857 1104 68-69 0.013601713787707581 1104 70-71 0.05092064304749755 1104 72-73 0.07644109651769782 1104 74-75 -0.03256277902752913 1104 76-77 -0.14467450401103576 1104 78-79 -0.15030270382703037 1104 80-81 -0.0865153693491898 1104 82-83 0.03394880060810479 1104 84-85 0.029642251769494976 1104 86-87 0.03860624067426954 1104 88-89 0.12163225192333016 1104 90-91 0.10266761643654476 1104 92-93 0.0605609059954908 1104 94-95 0.0447425523195939 1104 96-97 -0.1219902584603858 1104 98-99 0.04903133527978554 1105 1 0.017647700531515653 1105 2 0.05060446748132463 1105 3 0.005163294735307744 1105 4 0.0025902880079726742 1105 5 -0.008269608327260869 1105 6 -0.03338859531025662 1105 7 -0.05770977513167708 1105 8 -0.12544985892814964 1105 9 -0.12362164791534269 1105 10-11 -0.07901797431554058 1105 12-13 -0.11692798738113908 1105 14-15 -0.018318749581524685 1105 16-17 -0.07211689652271502 1105 18-19 -0.028075600612574192 1105 20-21 0.026271306233311975 1105 22-23 0.03259221311036242 1105 24-25 0.024754599454482218 1105 26-27 0.04763493778352057 1105 28-29 0.02127426060155102 1105 30-31 0.01555590260961992 1105 32-33 0.021733385279098627 1105 34-35 -0.04167096718271068 1105 36-37 0.015234711726705541 1105 38-39 -0.09880147671165673 1105 40-41 -0.10588872146975348 1105 42-43 -0.03822610160924711 1105 44-45 -0.12861405934026493 1105 46-47 -0.1988523974041243 1105 48-49 -0.14582461757707676 1105 50-51 -0.008403290467796865 1105 52-53 -0.07807881069085454 1105 54-55 0.007657534478546779 1105 56-57 -0.017021174181422793 1105 58-59 -0.08466057411729366 1105 60-61 0.021299243899761677 1105 62-63 0.017970769683032017 1105 64-65 -0.10388535196916848 1105 66-67 -0.08766063826256953 1105 68-69 -0.11717965202645075 1105 70-71 -0.18535581102788257 1105 72-73 -0.04328090769271853 1105 74-75 0.0016852017201642866 1105 76-77 -0.16011229382260694 1105 78-79 -0.1243310807713982 1105 80-81 -0.1085442991361667 1105 82-83 -0.0670309297154894 1105 84-85 -0.09784977143126738 1105 86-87 -0.09870624305540332 1105 88-89 -0.05757755904790329 1105 90-91 -0.01661805265438332 1105 92-93 -0.05986083998915248 1105 94-95 -0.05083719858575364 1105 96-97 -0.18165368845529883 1105 98-99 -0.07532510425463812 1106 1 -0.044834671696218464 1106 2 0.01948539641698943 1106 3 0.024259510237573778 1106 4 0.010988506387683117 1106 5 -0.028349838305452124 1106 6 -0.024477415593878504 1106 7 -0.07350944682266913 1106 8 -0.09038883906138295 1106 9 -0.04927847453100043 1106 10-11 -0.1395565530926106 1106 12-13 -0.06435994304733583 1106 14-15 -0.10659419538114179 1106 16-17 -0.07779297278288055 1106 18-19 -0.0013744145293372867 1106 20-21 -0.037086778562688494 1106 22-23 -0.013922487432125763 1106 24-25 0.005852197645999979 1106 26-27 0.011416067237874472 1106 28-29 -0.02571950126482392 1106 30-31 -0.031452016164607244 1106 32-33 0.06926555497710041 1106 34-35 -0.1406454257770804 1106 36-37 -0.07039408764906341 1106 38-39 -0.0737233738635581 1106 40-41 0.08774851098539216 1106 42-43 0.02649172605980965 1106 44-45 -0.03722631266590781 1106 46-47 -0.0504470871323619 1106 48-49 -0.11550301325753054 1106 50-51 -0.08547545003971635 1106 52-53 -0.07754554372311162 1106 54-55 -0.07136127023903072 1106 56-57 -0.05051125386106037 1106 58-59 0.008338763070653954 1106 60-61 0.016016154651211423 1106 62-63 -0.013310722991334956 1106 64-65 -0.12695024407837963 1106 66-67 -0.09079358853434627 1106 68-69 -0.017447478534514005 1106 70-71 -0.1550379646954596 1106 72-73 0.02047519559871347 1106 74-75 -0.10175011260209743 1106 76-77 -0.1255180143946859 1106 78-79 -0.03839845477718029 1106 80-81 -0.108159484379307 1106 82-83 -0.0010969439705910133 1106 84-85 -0.1732747919626938 1106 86-87 -0.12063380106411614 1106 88-89 -0.06040788588215307 1106 90-91 -0.09600326502346945 1106 92-93 -0.05933508118279818 1106 94-95 -0.0345149244850802 1106 96-97 -0.1494805996620805 1106 98-99 0.23070913239879332 1107 1 0.01780821175618641 1107 2 0.07172975298424333 1107 3 0.027762450038373743 1107 4 -0.04998970185008034 1107 5 0.0569646443383931 1107 6 -0.15993875853974515 1107 7 -0.17046926853770117 1107 8 -0.08132415562899098 1107 9 -0.12945605189435128 1107 10-11 -0.00858046462025186 1107 12-13 -0.07206016487175049 1107 14-15 -0.11839106837307156 1107 16-17 -0.06324570112914785 1107 18-19 -0.06710176422242853 1107 20-21 -0.11934944313832574 1107 22-23 -0.16593064614785646 1107 24-25 0.01314022675320814 1107 26-27 0.06536416540475187 1107 28-29 -0.014494093397722452 1107 30-31 -0.006462633307563692 1107 32-33 -0.11923251522437539 1107 34-35 -0.056548022434753875 1107 36-37 -0.06481411202025811 1107 38-39 -0.08650472891504535 1107 40-41 -0.17063147662203448 1107 42-43 -0.149642913171931 1107 44-45 -0.07520603551024863 1107 46-47 -0.21937470715659657 1107 48-49 -0.1199202621524762 1107 50-51 -0.12693367616116547 1107 52-53 -0.20191650547691609 1107 54-55 -0.1265474728862941 1107 56-57 -0.03560547047312923 1107 58-59 -0.014407488861138518 1107 60-61 0.0751396750051967 1107 62-63 -0.133709582005622 1107 64-65 -0.1677682744902924 1107 66-67 0.034431026008107324 1107 68-69 -0.106259284089262 1107 70-71 -0.029390175500275006 1107 72-73 -0.06984358488169562 1107 74-75 -0.1860419854935884 1107 76-77 -0.21689415150198954 1107 78-79 -0.07058392832770721 1107 80-81 -0.2679741202970831 1107 82-83 -0.22917301692935155 1107 84-85 -0.25530595062145167 1107 86-87 -0.21342132675756886 1107 88-89 -0.1637913004029521 1107 90-91 -0.09704594599920568 1107 92-93 -0.09780806769719774 1107 94-95 -0.05787598164725338 1107 96-97 -0.19099171307557583 1107 98-99 0.14260302495294042 1108 1 -0.06441140760463071 1108 2 0.0044890887896826825 1108 3 -0.0775110902974312 1108 4 -0.1272695785723741 1108 5 -0.04592493357576899 1108 6 -0.18900052651343202 1108 7 -0.15331646685417155 1108 8 -0.2616021139848712 1108 9 -0.21240970316745944 1108 10-11 -0.25540208587165836 1108 12-13 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68-69 -0.22364411991290467 2214 70-71 -0.17138213266323987 2214 72-73 -0.19457154928090858 2214 74-75 -0.15147643435228986 2214 76-77 -0.15479977827751767 2214 78-79 -0.06815549900729678 2214 80-81 -0.011856502867377827 2214 82-83 -0.011163795114441655 2214 84-85 -0.03938092498035317 2214 86-87 -0.08446748433566142 2214 88-89 -0.046431926039485205 2214 90-91 0.08050135755465604 2214 92-93 0.0636239135077723 2214 94-95 -0.030447679815239326 2214 96-97 0.02170298850104757 2214 98-99 -0.0644619413353631 2215 1 -0.12229393355416462 2215 2 -0.13547107920192403 2215 3 -0.1200665844151878 2215 4 -0.1303303469904833 2215 5 -0.11852664982717442 2215 6 -0.0827310690045664 2215 7 -0.10141605475681814 2215 8 -0.06051491205023041 2215 9 -0.08217112995512821 2215 10-11 -0.23031262824385124 2215 12-13 -0.08039377091031952 2215 14-15 -0.15581876490242053 2215 16-17 -0.060936448371521124 2215 18-19 -0.14211564938497645 2215 20-21 -0.09108916079205187 2215 22-23 -0.2185002107837306 2215 24-25 -0.12406092868843643 2215 26-27 -0.11159487681491953 2215 28-29 -0.04445867896424005 2215 30-31 -0.16411018232979302 2215 32-33 -0.1961041074303509 2215 34-35 -0.19295105896102172 2215 36-37 -0.1136878032774149 2215 38-39 -0.14486401580548147 2215 40-41 -0.09359844196460188 2215 42-43 -0.02698776964137295 2215 44-45 -0.04347774069030663 2215 46-47 0.03642931074365663 2215 48-49 0.062067897005064765 2215 50-51 0.041972869710953375 2215 52-53 0.03120106385169663 2215 54-55 0.09850428789827248 2215 56-57 0.09602748108427761 2215 58-59 -0.034524127647259206 2215 60-61 -0.03616766193636067 2215 62-63 0.010522864680311272 2215 64-65 -0.05946972200209899 2215 66-67 -0.21222982186991857 2215 68-69 0.018918734469821175 2215 70-71 -0.0977667477219839 2215 72-73 -0.0933018272863535 2215 74-75 0.006110862352969093 2215 76-77 0.025363366696382172 2215 78-79 0.12747733981664666 2215 80-81 0.14989843563210314 2215 82-83 0.05702995752274731 2215 84-85 0.07704044630001405 2215 86-87 0.06655048502268102 2215 88-89 -0.051588882155513716 2215 90-91 0.16240567075137946 2215 92-93 0.07100503624751653 2215 94-95 0.03704649290934725 2215 96-97 0.12779633999488027 2215 98-99 0.10816542734204404 2216 1 -0.03155963380836013 2216 2 -0.013173733100227736 2216 3 0.040720630295218996 2216 4 0.043239150927647074 2216 5 -0.025196268374116926 2216 6 0.027826089599081172 2216 7 0.08148630748987529 2216 8 0.08213382454029272 2216 9 0.05851119858657228 2216 10-11 0.03367413413103293 2216 12-13 0.00234236459772319 2216 14-15 -0.06629264919532574 2216 16-17 -0.06249284849044301 2216 18-19 -0.10494447685204022 2216 20-21 -0.09970549459538347 2216 22-23 -0.2396728145365472 2216 24-25 -0.16106128443335166 2216 26-27 -0.24189917455603904 2216 28-29 -0.053381969904748416 2216 30-31 -0.15228082040846402 2216 32-33 -0.11162316733673805 2216 34-35 -0.2365691521759743 2216 36-37 -0.2631428588401832 2216 38-39 -0.15884064852723867 2216 40-41 -0.21375387017758385 2216 42-43 -0.14456152362322428 2216 44-45 -0.11885725987716 2216 46-47 -0.015510966855835306 2216 48-49 0.02976837948169475 2216 50-51 -0.02097234093626099 2216 52-53 0.06252614865816497 2216 54-55 0.06193483993636306 2216 56-57 0.17140355290586484 2216 58-59 0.00632588254354971 2216 60-61 0.0739471105420364 2216 62-63 0.10484867204939263 2216 64-65 0.16031247983364239 2216 66-67 0.0281626769207719 2216 68-69 -0.03906381523114533 2216 70-71 -0.04883809936341521 2216 72-73 -0.02121249153537974 2216 74-75 0.026436795127608548 2216 76-77 -0.04765750444477135 2216 78-79 -0.033377206347211086 2216 80-81 0.06663911174830517 2216 82-83 0.04159523414049815 2216 84-85 0.05940287904420316 2216 86-87 -0.001449908575665404 2216 88-89 -0.07879376354748047 2216 90-91 0.11994503927646605 2216 92-93 0.2453795277530908 2216 94-95 0.19383061484062836 2216 96-97 0.18390196507784395 2216 98-99 0.20277318798893162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 8.0 7 198.0 8 1386.0 9 2464.0 10 3819.0 11 7089.0 12 14009.0 13 24571.0 14 34491.0 15 37738.0 16 39726.0 17 41568.0 18 43057.0 19 45745.0 20 49259.0 21 53787.0 22 59761.0 23 67440.0 24 76729.0 25 86718.0 26 95444.0 27 107960.0 28 127856.0 29 157340.0 30 196202.0 31 249423.0 32 327751.0 33 437564.0 34 596939.0 35 861705.0 36 1329806.0 37 1647818.0 38 520516.0 39 424.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.12138370541667 18.594138787358126 12.309670945047609 24.974806562177594 2 16.08623921397273 20.531001278192726 37.62830591140314 25.754453596431404 3 18.09005401629282 26.228924430277985 28.687957560143868 26.993063993285325 4 12.945912581158082 17.3165688193707 36.39302773868408 33.34449086078713 5 14.467492595943732 37.46830211789291 33.05426356167061 15.009941724492743 6 31.80853628440179 36.70802937692129 17.51940531785273 13.964029020824192 7 28.055196683069912 31.83641422204968 21.8241373119107 18.28425178296971 8 27.129534810056366 33.129730010069 19.815401226547582 19.92533395332705 9 27.112138323574918 15.4526401073954 19.23580420159179 38.19941736743789 10-11 25.613386092693325 25.71493365853964 26.398834734875777 22.272845513891255 12-13 26.37301225063845 23.784189098446827 25.927924913606297 23.914873737308426 14-15 24.473119910115432 24.45926261493694 23.410423272306332 27.6571942026413 16-17 24.21702187901137 26.764899657106767 24.490417638242874 24.527660825638993 18-19 24.725947485751693 26.459913009400225 25.5350950973897 23.279044407458382 20-21 26.05153311833579 25.65770823908219 24.371425531991644 23.91933311059038 22-23 25.384298323335347 25.554900956412002 24.141470460480097 24.919330259772558 24-25 24.743832262814106 26.207703327866387 24.232000466106033 24.816463943213474 26-27 24.886146922740803 25.890937082698663 24.18817065982786 25.034745334732676 28-29 24.476051689685185 25.97835710377178 24.784976292410597 24.760614914132436 30-31 25.050836999194214 25.89306673648892 24.476198714899468 24.5798975494174 32-33 24.463101130104928 26.20604442312459 24.073286326507638 25.257568120262842 34-35 24.899570266986583 26.196984320894362 24.683577343741014 24.219868068378045 36-37 24.900490872221447 26.026818085975396 24.365732678619242 24.70695836318392 38-39 24.92614487943162 25.71919624665912 24.519273863873263 24.835385010035992 40-41 24.744466984858114 25.965712586902463 24.75081711079207 24.539003317447357 42-43 25.27891209615275 25.785982923946456 24.54817118414943 24.386933795751364 44-45 24.668408402530197 26.14583700581149 24.46424470730956 24.72150988434876 46-47 24.318593372918734 25.992671696039004 25.01188011234482 24.67685481869744 48-49 24.903131468820277 26.027201187210373 24.700764208144594 24.368903135824755 50-51 24.093146886920525 26.266339663539974 24.606506585414095 25.03400686412541 52-53 24.140388284677847 26.094852232637578 24.410993490474333 25.353765992210242 54-55 24.41836453697645 25.899822373433413 24.458772573064223 25.223040516525913 56-57 24.720747596991195 25.46637353087829 24.324732508601937 25.48814636352858 58-59 24.392086041552012 25.519638359987756 24.998744267701163 25.08953133075907 60-61 24.52976058323695 25.72711664398635 24.686083940633605 25.057038832143096 62-63 24.09835358181814 25.789460860015318 24.50840292495104 25.6037826332155 64-65 24.046449980132888 25.636499734356466 24.683238975317 25.63381131019365 66-67 24.874966096913315 25.469884019965132 24.443967258381168 25.211182624740385 68-69 24.715248238197375 25.26097111870162 24.560007056602966 25.46377358649804 70-71 24.239342712281033 25.46375316808668 24.652679419643412 25.644224699988875 72-73 24.95647135004222 25.43825737843116 24.649868485012412 24.95540278651421 74-75 25.106927817240514 25.38458418109443 24.691637748524396 24.816850253140657 76-77 24.702731752031735 25.569581794182138 24.837962890490207 24.889723563295917 78-79 24.692590607721346 25.557548543752095 24.838970198784125 24.910890649742434 80-81 24.41561485757954 25.455245496685343 25.19431181173789 24.93482783399723 82-83 24.80091708614024 25.373435728490122 24.876832739588618 24.948814445781018 84-85 24.919459576377857 25.60295228448673 24.427273770467924 25.050314368667486 86-87 24.644164942104958 25.214260599639736 24.7604546009555 25.381119857299804 88-89 24.829836362767654 24.758058840688886 24.938006300032765 25.4740984965107 90-91 24.98853506201956 24.993333388689916 24.766233011371284 25.25189853791924 92-93 24.930424263279896 25.156748740966723 24.677276799198943 25.235550196554435 94-95 24.237858974388644 25.255226738971437 25.174471922029984 25.332442364609935 96-97 24.81550944412781 25.161608322871164 24.629000868599217 25.393881364401803 98-99 24.6620786950076 25.399972857125164 24.531325994774793 25.406622453092442 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 895.0 1 833.0 2 815.5 3 837.5 4 770.0 5 703.0 6 633.5 7 562.0 8 547.5 9 552.0 10 537.5 11 526.0 12 519.5 13 549.0 14 625.5 15 768.5 16 908.0 17 1059.0 18 1378.0 19 1800.5 20 2356.0 21 3345.5 22 5017.5 23 7003.5 24 9077.0 25 12289.5 26 16966.5 27 22057.5 28 28905.0 29 38355.0 30 47038.0 31 57998.5 32 70089.5 33 79908.0 34 89645.5 35 101699.5 36 110723.0 37 123155.5 38 137996.0 39 135954.0 40 135254.0 41 144778.5 42 156978.0 43 177995.0 44 202101.0 45 227798.0 46 263265.0 47 297989.5 48 321286.0 49 340582.0 50 361737.0 51 351301.5 52 331910.5 53 331654.0 54 316387.5 55 306528.5 56 290736.5 57 265533.5 58 252132.0 59 227526.0 60 191051.5 61 151393.0 62 123334.0 63 100072.0 64 75582.5 65 58277.0 66 43204.5 67 32705.0 68 25602.0 69 21403.5 70 18947.5 71 16881.5 72 15846.5 73 14041.5 74 10654.0 75 8122.0 76 6147.0 77 4436.0 78 3025.5 79 2055.0 80 1468.5 81 1017.5 82 712.5 83 521.0 84 373.0 85 283.0 86 206.5 87 156.5 88 108.0 89 71.5 90 51.0 91 31.5 92 21.5 93 17.5 94 14.0 95 11.0 96 10.0 97 6.0 98 7.0 99 10.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0020690656848042508 2 1.3612274242133229E-5 3 1.2251046817919904E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.3612274242133229E-5 18-19 0.0 20-21 3.4711299317439733E-4 22-23 0.0 24-25 0.004669010065051698 26-27 1.3612274242133229E-5 28-29 0.009086193056623929 30-31 5.10460284079996E-4 32-33 2.7224548484266457E-5 34-35 7.486750833173276E-5 36-37 0.0 38-39 3.403068560533307E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.9057183938986518E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.7642959847466295E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7346311.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.93561127967534 #Duplication Level Percentage of deduplicated Percentage of total 1 74.24037893626078 28.905945355180467 2 13.835223135156294 10.773657399160331 3 4.891207169634596 5.713264231357609 4 2.20336301466339 3.431571433877894 5 1.1672977607820025 2.272472593072175 6 0.7301364393077708 1.7056985149208177 7 0.4844648929625936 1.3204055725728745 8 0.35446615516250723 1.1041085143366773 9 0.2570802673561195 0.9008619621707591 >10 1.51571087371958 11.039768797892044 >50 0.145478208875051 3.9481449368651598 >100 0.13682560545152245 11.473649607812856 >500 0.024269126270952946 6.522212117753531 >1k 0.01367756620611654 9.44881241222935 >5k 3.5070682572578045E-4 0.8842831502750683 >10k+ 7.014136514515609E-5 0.5551434005224432 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 21083 0.2869875778468949 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 19572 0.2664194314670315 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 7939 0.10806784520829571 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.3612274242133229E-5 2.7224548484266457E-5 2 0.0 0.0 0.0 1.3612274242133229E-5 2.7224548484266457E-5 3 0.0 0.0 0.0 1.3612274242133229E-5 2.7224548484266457E-5 4 0.0 0.0 0.0 1.3612274242133229E-5 2.7224548484266457E-5 5 1.3612274242133229E-5 4.083682272639968E-5 0.0 2.7224548484266457E-5 4.083682272639968E-5 6 1.3612274242133229E-5 4.083682272639968E-5 0.0 2.7224548484266457E-5 4.083682272639968E-5 7 1.3612274242133229E-5 4.083682272639968E-5 0.0 2.7224548484266457E-5 4.083682272639968E-5 8 1.3612274242133229E-5 4.083682272639968E-5 0.0 2.7224548484266457E-5 4.083682272639968E-5 9 1.3612274242133229E-5 4.083682272639968E-5 0.0 2.7224548484266457E-5 4.083682272639968E-5 10-11 1.3612274242133229E-5 4.083682272639968E-5 0.0 4.7642959847466295E-5 4.083682272639968E-5 12-13 1.3612274242133229E-5 5.4449096968532914E-5 0.0 5.4449096968532914E-5 2.1099025075306505E-4 14-15 2.0418411363199845E-5 8.167364545279936E-5 0.0 6.125523408959953E-5 2.7905162196373116E-4 16-17 2.7224548484266457E-5 8.167364545279936E-5 0.0 8.167364545279936E-5 3.2669458181119746E-4 18-19 4.083682272639968E-5 8.167364545279936E-5 0.0 1.2251046817919907E-4 3.5391913029546394E-4 20-21 8.167364545279936E-5 8.167364545279936E-5 0.0 2.5863321060053135E-4 4.0156209014293023E-4 22-23 8.847978257386598E-5 9.52859196949326E-5 0.0 0.001129818762097058 4.423989128693299E-4 24-25 1.0889819393706583E-4 9.52859196949326E-5 0.0 0.0033418133264437075 4.628173242325298E-4 26-27 1.4292887954239888E-4 9.52859196949326E-5 0.0 0.005900920883964754 4.8323573559572966E-4 28-29 1.5654115378453212E-4 9.52859196949326E-5 0.0 0.01690644460872947 5.172664212010627E-4 30-31 1.7695956514773197E-4 9.52859196949326E-5 0.0 0.04997746487999215 5.240725583221293E-4 32-33 2.1099025075306505E-4 9.52859196949326E-5 0.0 0.11055208525748503 5.308786954431959E-4 34-35 2.1779638787413166E-4 1.0889819393706583E-4 0.0 0.18789022136416494 5.581032439274623E-4 36-37 2.1779638787413166E-4 1.1570433105813245E-4 0.0 0.3078075513002376 5.581032439274623E-4 38-39 2.1779638787413166E-4 1.2931660530026567E-4 0.0 0.4934313290030874 5.717155181695956E-4 40-41 2.1779638787413166E-4 1.3612274242133228E-4 0.0 0.7250577875072264 5.717155181695956E-4 42-43 2.2460252499519826E-4 1.4292887954239888E-4 0.0 0.9312360993156974 5.921339295327955E-4 44-45 2.5863321060053135E-4 1.4973501666346552E-4 0.0 1.1625494755122672 6.670014378645282E-4 46-47 2.5863321060053135E-4 1.6334729090559873E-4 0.0 1.4219926164302055 7.486750833173275E-4 48-49 2.5863321060053135E-4 1.6334729090559873E-4 0.0 1.6929585474941096 7.827057689226606E-4 50-51 2.5863321060053135E-4 1.6334729090559873E-4 0.0 1.9913872418415175 8.439610030122602E-4 52-53 2.5863321060053135E-4 1.6334729090559873E-4 0.0 2.2911567452017754 9.120223742229263E-4 54-55 2.5863321060053135E-4 1.7015342802666536E-4 0.0 2.584467224434141 9.392469227071928E-4 56-57 2.5863321060053135E-4 1.905718393898652E-4 0.0 2.916654358902039 9.732776083125258E-4 58-59 2.5863321060053135E-4 2.0418411363199842E-4 0.0 3.2789654562677786 9.800837454335925E-4 60-61 2.5863321060053135E-4 2.1099025075306505E-4 0.0 3.6454351034144894 0.0010005021567967925 62-63 2.5863321060053135E-4 2.1779638787413166E-4 0.0 4.013265977985413 0.0010481451166442586 64-65 2.5863321060053135E-4 2.2460252499519826E-4 0.0 4.397873980559766 0.001075369665128525 66-67 2.6543934772159795E-4 2.4502093635839813E-4 0.0 4.815478135897051 0.001075369665128525 68-69 2.7224548484266456E-4 2.4502093635839813E-4 0.0 5.2674464775586 0.001075369665128525 70-71 2.7224548484266456E-4 2.7905162196373116E-4 0.0 5.778219843946166 0.0010821758022495917 72-73 2.7224548484266456E-4 3.1308230756906425E-4 0.0 6.266281130760731 0.001143431036339191 74-75 2.7905162196373116E-4 3.1308230756906425E-4 0.0 6.750823644683706 0.0012114924075498574 76-77 2.9947003332693104E-4 3.2669458181119746E-4 0.0 7.266994277808276 0.0012251046817919906 78-79 3.1308230756906425E-4 3.403068560533307E-4 0.0 7.801561899571091 0.0012251046817919906 80-81 3.1308230756906425E-4 3.5391913029546394E-4 0.0 8.38925822770095 0.0012319108189130573 82-83 3.1308230756906425E-4 3.5391913029546394E-4 0.0 9.01083550641948 0.0012863599158815901 84-85 3.1308230756906425E-4 3.5391913029546394E-4 0.0 9.617847651698927 0.0012931660530026566 86-87 3.1308230756906425E-4 4.219805015061301E-4 0.0 10.236852210585695 0.0013271967386079898 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACT 3430 0.0 23.995106 4 GTACTAG 2715 0.0 22.608475 1 GTATAAG 3115 0.0 22.243103 1 GTGATCG 2415 0.0 20.409494 8 CTAGTAC 4105 0.0 20.389326 3 TAGGCAT 4045 0.0 19.657175 5 GTACAAG 3615 0.0 19.552507 1 GTACATA 1955 0.0 19.504475 1 TGATCGC 2505 0.0 19.490595 9 ACGTTAA 780 0.0 19.076508 8 CTAGGCA 4475 0.0 18.911322 4 CTGTGCG 4375 0.0 18.068178 9 ATAAGGT 2985 0.0 17.914185 3 TAAGGTG 3025 0.0 17.677301 4 CGTTAAC 955 0.0 17.529367 1 CTATTGA 3240 0.0 17.365362 9 CATGGGG 17800 0.0 17.293444 4 GTACATG 59030 0.0 16.858082 1 GTACTGT 5000 0.0 16.832634 6 GTATAGA 1665 0.0 16.757305 1 >>END_MODULE