FastQCFastQC Report
Fri 27 May 2016
ERR765329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765329_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7346311
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT217320.2958219438300393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207550.28252275189547515No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA205120.2792149692546368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128380.1747543767205064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107560.14641362174838501No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG79550.10828564159616984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA527950.025.9812031
TATCACG5600.025.741572
GTACTAG28650.023.061541
AGTACTT314950.022.82154312-13
CAACGCA625650.021.5614385
ATCAACG633000.021.325773
AACGCAG654300.020.9219256
TATCAAC657000.020.8794022
TCAACGC657250.020.5601564
TACTTTT358950.020.09561714-15
GAGTACT299900.019.70224812-13
ACGCAGA695450.019.4566387
CGCAGAG699150.019.333728
GCAGAGT731300.018.4083299
CATGGGG157350.018.1452544
TAGGCAT43950.017.8808965
TATTCGC5250.017.714639
ATACGGG24200.017.4858783
GTACTTT340250.017.37284514-15
CTATTGA34900.017.3212559
Produced by FastQC (version 0.11.5)