##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765329_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7346311 Sequences flagged as poor quality 0 Sequence length 99 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.361404519901214 33.0 33.0 33.0 33.0 33.0 2 32.3163756611992 33.0 33.0 33.0 33.0 33.0 3 32.37153096295542 33.0 33.0 33.0 33.0 33.0 4 36.24062920831966 37.0 37.0 37.0 33.0 37.0 5 36.17217171992855 37.0 37.0 37.0 33.0 37.0 6 36.17663627363448 37.0 37.0 37.0 33.0 37.0 7 36.06703214715522 37.0 37.0 37.0 33.0 37.0 8 36.08351824473535 37.0 37.0 37.0 33.0 37.0 9 36.11192393025561 37.0 37.0 37.0 33.0 37.0 10-11 36.066706813800835 37.0 37.0 37.0 33.0 37.0 12-13 36.037098892219504 37.0 37.0 37.0 33.0 37.0 14-15 37.523797182014206 38.5 37.0 40.0 33.0 40.0 16-17 37.52125134642408 40.0 37.0 40.0 33.0 40.0 18-19 37.56833946997344 40.0 37.0 40.0 33.0 40.0 20-21 37.550554080816894 40.0 37.0 40.0 33.0 40.0 22-23 37.523258067892854 40.0 37.0 40.0 33.0 40.0 24-25 37.49962736399262 40.0 37.0 40.0 33.0 40.0 26-27 37.367660925327016 40.0 37.0 40.0 33.0 40.0 28-29 37.270859483079334 37.0 37.0 40.0 33.0 40.0 30-31 37.11628720864118 37.0 37.0 40.0 33.0 40.0 32-33 36.998696624741314 37.0 37.0 40.0 33.0 40.0 34-35 36.904444625336446 37.0 37.0 40.0 33.0 40.0 36-37 36.75789610595032 37.0 37.0 40.0 33.0 40.0 38-39 36.643925774446515 37.0 37.0 40.0 33.0 40.0 40-41 36.47761529562253 37.0 37.0 40.0 33.0 40.0 42-43 36.320736829682275 37.0 37.0 40.0 33.0 40.0 44-45 36.03246683947903 37.0 37.0 40.0 33.0 40.0 46-47 35.795985903673284 37.0 37.0 40.0 27.0 40.0 48-49 35.52582425383298 37.0 37.0 40.0 27.0 40.0 50-51 35.324411326991196 37.0 35.0 40.0 27.0 40.0 52-53 35.16423977966629 37.0 33.0 40.0 27.0 40.0 54-55 34.9690000191933 37.0 33.0 40.0 27.0 40.0 56-57 34.759212480930906 37.0 33.0 40.0 27.0 40.0 58-59 34.541302158321365 37.0 33.0 38.5 27.0 40.0 60-61 34.371287643553345 37.0 33.0 37.0 27.0 40.0 62-63 34.15384374552071 37.0 33.0 37.0 27.0 40.0 64-65 33.936923035248576 37.0 33.0 37.0 27.0 40.0 66-67 33.71027152267308 37.0 33.0 37.0 27.0 40.0 68-69 33.5031109491553 37.0 33.0 37.0 27.0 40.0 70-71 33.32160576920852 37.0 33.0 37.0 27.0 37.0 72-73 33.11379970164617 37.0 33.0 37.0 27.0 37.0 74-75 32.88056202630136 37.0 33.0 37.0 27.0 37.0 76-77 32.70452632620645 37.0 33.0 37.0 22.0 37.0 78-79 32.544864014060934 33.0 33.0 37.0 22.0 37.0 80-81 32.3831761955082 33.0 33.0 37.0 22.0 37.0 82-83 32.286511692739396 33.0 33.0 37.0 22.0 37.0 84-85 32.16289366186648 33.0 33.0 37.0 22.0 37.0 86-87 32.05425532624469 33.0 33.0 37.0 22.0 37.0 88-89 31.940539612330596 33.0 33.0 37.0 22.0 37.0 90-91 31.839999014471346 33.0 33.0 37.0 22.0 37.0 92-93 31.75987744052763 33.0 33.0 37.0 22.0 37.0 94-95 31.6610621303672 33.0 33.0 37.0 22.0 37.0 96-97 31.60610046321208 33.0 33.0 37.0 22.0 37.0 98-99 30.802645368539395 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.02904020952660602 1101 2 0.05035855005095158 1101 3 0.0496238824434343 1101 4 0.13002122211533873 1101 5 0.06355679582758711 1101 6 0.07512756914454144 1101 7 0.08019816936441515 1101 8 0.07680878870338148 1101 9 0.08663368141036898 1101 10-11 0.05838384202615288 1101 12-13 0.03742199035886529 1101 14-15 0.08982750700059938 1101 16-17 0.15346337306365854 1101 18-19 0.15470128472122724 1101 20-21 0.18157632153469194 1101 22-23 0.12647813022221044 1101 24-25 0.15857126923874176 1101 26-27 0.15924285646593006 1101 28-29 0.12974714129821763 1101 30-31 0.14258768216487994 1101 32-33 0.20645430781110008 1101 34-35 0.250178170350857 1101 36-37 0.22509129578325116 1101 38-39 0.15227467409029316 1101 40-41 0.051743870386076196 1101 42-43 0.1601277525134961 1101 44-45 0.25831955783509386 1101 46-47 0.26476227419254883 1101 48-49 0.1953876046336589 1101 50-51 0.2488273006683812 1101 52-53 0.2876538299483755 1101 54-55 0.19628360503988773 1101 56-57 0.2358304840904566 1101 58-59 0.24130672096637085 1101 60-61 0.14741493132088124 1101 62-63 0.19134813570446596 1101 64-65 0.18648246482278097 1101 66-67 0.3382594152346172 1101 68-69 0.2720097534649213 1101 70-71 0.17545698175281643 1101 72-73 0.20506785439446418 1101 74-75 0.28217477880333064 1101 76-77 0.32174779434365064 1101 78-79 0.2733602534634372 1101 80-81 0.20614375888930425 1101 82-83 0.18031872775455327 1101 84-85 0.1850144832074534 1101 86-87 0.16963983296685115 1101 88-89 0.21497850389264528 1101 90-91 0.26077617877450265 1101 92-93 0.24951178816411002 1101 94-95 0.311281398170717 1101 96-97 0.3005334657439285 1101 98-99 0.34132120123255305 1102 1 -0.0064163132027985625 1102 2 0.07160524618819153 1102 3 0.07442678358997767 1102 4 0.1002208040893322 1102 5 0.11731559400467262 1102 6 0.0814762849483941 1102 7 0.10492834126618789 1102 8 0.1300357991612131 1102 9 0.09753875934543998 1102 10-11 0.07587229096542814 1102 12-13 0.05665227623283897 1102 14-15 0.1514295487682844 1102 16-17 0.06584703134466707 1102 18-19 0.15800056011556762 1102 20-21 0.123261520291436 1102 22-23 0.1700960894673358 1102 24-25 0.18228350410790028 1102 26-27 0.16325190440990411 1102 28-29 0.08234742228519565 1102 30-31 0.08842421767746345 1102 32-33 0.1457827521642514 1102 34-35 0.18500700961366334 1102 36-37 0.16104456581960847 1102 38-39 0.18765232570829937 1102 40-41 0.10583864201512938 1102 42-43 0.11731882873173305 1102 44-45 0.2145482282877893 1102 46-47 0.2078470094582201 1102 48-49 0.2133443610678185 1102 50-51 0.22286323267929475 1102 52-53 0.21497981928711596 1102 54-55 0.07072724922326046 1102 56-57 0.11903109336194007 1102 58-59 0.18198469273421125 1102 60-61 0.18407156026219695 1102 62-63 0.24729653731402124 1102 64-65 0.15313838430108007 1102 66-67 0.21935934539027357 1102 68-69 0.26057795186594035 1102 70-71 0.18397360850754296 1102 72-73 0.09580094264732963 1102 74-75 0.24392546111978675 1102 76-77 0.32956623768275506 1102 78-79 0.2436094978481833 1102 80-81 0.26006646407052614 1102 82-83 0.28792131109138097 1102 84-85 0.23535300106864554 1102 86-87 0.2213764386952306 1102 88-89 0.2884515581036666 1102 90-91 0.18389678587672975 1102 92-93 0.22626810842091416 1102 94-95 0.2585393112524592 1102 96-97 0.32117226475305927 1102 98-99 0.26054386006962105 1103 1 -0.03586539811372802 1103 2 0.04839466230834688 1103 3 0.061450080356074466 1103 4 0.06713519293547421 1103 5 0.015846020607860112 1103 6 0.03917227408209101 1103 7 0.027246656645090184 1103 8 0.07314113679093026 1103 9 0.05119457908555347 1103 10-11 0.09807161018349575 1103 12-13 0.04081900026512386 1103 14-15 0.10131662160623733 1103 16-17 0.061695045614477806 1103 18-19 0.1162445145697859 1103 20-21 0.08606825101786342 1103 22-23 0.057030901284996105 1103 24-25 0.11016909411551978 1103 26-27 0.11573926958144654 1103 28-29 0.09714540489648016 1103 30-31 0.1694480192610186 1103 32-33 0.09282597000336068 1103 34-35 0.135269630637886 1103 36-37 0.09474938106947661 1103 38-39 0.053313399409461226 1103 40-41 -8.266349035181975E-4 1103 42-43 0.026148569274532463 1103 44-45 0.021017971551714254 1103 46-47 0.03645555627882402 1103 48-49 0.059735423025855994 1103 50-51 0.1714485437886566 1103 52-53 0.023607839607279857 1103 54-55 0.03739148342548049 1103 56-57 0.040721784263730854 1103 58-59 0.10214597566784533 1103 60-61 0.022066780631718075 1103 62-63 0.07075513932318955 1103 64-65 0.09427440682115673 1103 66-67 0.18333013603283632 1103 68-69 0.10523094500128138 1103 70-71 -0.060700332200809726 1103 72-73 0.03994465475510367 1103 74-75 0.12941884315430485 1103 76-77 0.08726641470940422 1103 78-79 0.1133743093471935 1103 80-81 0.16210129761009284 1103 82-83 0.032020103689006874 1103 84-85 0.025482575206552838 1103 86-87 0.034443143113747965 1103 88-89 0.13435620014584515 1103 90-91 0.07207692624777096 1103 92-93 0.09078807250801546 1103 94-95 0.05187701815686907 1103 96-97 0.06473753276077332 1103 98-99 0.0827880501485545 1104 1 -0.01738740616377754 1104 2 0.021572260314741243 1104 3 -0.028290204617213988 1104 4 -0.05659025672715501 1104 5 -0.027692446798077697 1104 6 0.027874751450262636 1104 7 0.08847773848176388 1104 8 0.10448567062682201 1104 9 -0.016905650227776903 1104 10-11 -4.6681399005166213E-4 1104 12-13 -0.05467287907878671 1104 14-15 0.04378919252329183 1104 16-17 0.09575051362467946 1104 18-19 0.06761471189585677 1104 20-21 0.08172315701030186 1104 22-23 0.06841545646746994 1104 24-25 0.050956490173476254 1104 26-27 -0.005987460927805444 1104 28-29 -0.014534670812267336 1104 30-31 -0.07687384969268152 1104 32-33 0.028609627068320265 1104 34-35 -0.036328758898172 1104 36-37 0.1042552052180099 1104 38-39 0.029036764411344507 1104 40-41 -0.00472298405104965 1104 42-43 0.06531912197568346 1104 44-45 0.02986244564904439 1104 46-47 0.02848947421837522 1104 48-49 0.058302468174076694 1104 50-51 -0.05926843344578003 1104 52-53 0.03945704702624653 1104 54-55 -0.07779862802858162 1104 56-57 0.084696509427026 1104 58-59 0.07341199251634833 1104 60-61 0.06506068626049455 1104 62-63 -0.049054944620152696 1104 64-65 -0.046583436935968336 1104 66-67 0.044938493026506876 1104 68-69 0.05708210464229069 1104 70-71 -0.11671914164645614 1104 72-73 0.06523737247636063 1104 74-75 0.10923435620923527 1104 76-77 0.03244770545907727 1104 78-79 0.008582912744365956 1104 80-81 0.05016496660101666 1104 82-83 0.08354517028837449 1104 84-85 0.11763903546408017 1104 86-87 0.09445688046780276 1104 88-89 0.029578117982158147 1104 90-91 0.028685891009285314 1104 92-93 0.0772686202138324 1104 94-95 -0.012944290227714816 1104 96-97 0.08930838725803625 1104 98-99 0.09957142992601575 1105 1 -0.018361285333476474 1105 2 0.09701623141965854 1105 3 0.04778450363755127 1105 4 0.10573173281146353 1105 5 0.06347676423838777 1105 6 0.07000858598974702 1105 7 0.05013518259236349 1105 8 0.06766198180451255 1105 9 0.016248471679332965 1105 10-11 0.03274146109961151 1105 12-13 -0.015109387243548156 1105 14-15 0.028142326473982848 1105 16-17 0.04803376148346672 1105 18-19 0.06495184877729798 1105 20-21 0.07296988959239314 1105 22-23 -0.029040180177148045 1105 24-25 0.02937449332087283 1105 26-27 0.044421442325884186 1105 28-29 -0.010841173217400524 1105 30-31 0.0350552320019446 1105 32-33 0.014807517942841741 1105 34-35 0.07430653543291754 1105 36-37 0.07211178293891862 1105 38-39 0.03840279579398498 1105 40-41 -0.03527220253490526 1105 42-43 -0.040991655646784864 1105 44-45 0.010731262972058175 1105 46-47 -0.02721326515444389 1105 48-49 0.010506657381135653 1105 50-51 0.01769264043777241 1105 52-53 -0.06669449752128287 1105 54-55 -0.05205475930556247 1105 56-57 -0.07856758597692703 1105 58-59 4.994369841568869E-4 1105 60-61 -0.08061642194859786 1105 62-63 -0.02427594430594837 1105 64-65 -0.021891797096550647 1105 66-67 0.04519677196618943 1105 68-69 -0.018531012582947426 1105 70-71 -0.038102033310721595 1105 72-73 0.02455174082056999 1105 74-75 0.05156962334773141 1105 76-77 -0.03472488663078366 1105 78-79 -0.011946162399027571 1105 80-81 -0.07536551828135885 1105 82-83 -0.05308998376969498 1105 84-85 -0.13120098515892664 1105 86-87 0.013529359870382507 1105 88-89 0.03251424843612938 1105 90-91 0.036676542729548345 1105 92-93 -0.004328894965535568 1105 94-95 0.0060898646404936585 1105 96-97 -0.03436281150314002 1105 98-99 0.11934258608486559 1106 1 -0.03502207516455513 1106 2 0.04149171376472793 1106 3 0.02394036438020919 1106 4 0.01905870522421793 1106 5 0.03912611706925162 1106 6 0.032834592935756746 1106 7 0.01831870412038228 1106 8 0.025717845212916757 1106 9 0.03244706815786458 1106 10-11 0.015922288908697624 1106 12-13 4.989786827067633E-4 1106 14-15 0.015437306400080786 1106 16-17 0.06713956336869842 1106 18-19 -0.01026995918608975 1106 20-21 -0.04020925251337104 1106 22-23 -0.0108711093206324 1106 24-25 0.021794547340249437 1106 26-27 4.6352465639643015E-4 1106 28-29 -0.015637185144576904 1106 30-31 0.020558136465034238 1106 32-33 -0.01661373122583143 1106 34-35 0.041890060329841106 1106 36-37 0.09169550547002814 1106 38-39 -0.03738069452008119 1106 40-41 -0.026382125361635644 1106 42-43 -0.0637335093072906 1106 44-45 -0.0015362133322298632 1106 46-47 0.03680397527169532 1106 48-49 -0.04362324859292954 1106 50-51 -0.06966830974138816 1106 52-53 -0.07822136128523027 1106 54-55 -0.0760651797272871 1106 56-57 0.03972468435450338 1106 58-59 0.02171611820931929 1106 60-61 -0.10478745139612045 1106 62-63 -0.0940207868241032 1106 64-65 -0.045759570264628735 1106 66-67 -0.03016376900644957 1106 68-69 -0.08097460957134217 1106 70-71 -0.10961250475043727 1106 72-73 -0.052224436247406913 1106 74-75 -0.012208848170622844 1106 76-77 0.006570993274635839 1106 78-79 -0.10251140991537966 1106 80-81 -0.10240276685600236 1106 82-83 -0.15289398872455706 1106 84-85 -0.09161045012375268 1106 86-87 -0.02587964329173076 1106 88-89 -0.003883528860587404 1106 90-91 -0.14300793198338724 1106 92-93 -0.03411183362896608 1106 94-95 -0.09526697028492848 1106 96-97 -0.06131686871198383 1106 98-99 -0.015322476319756362 1107 1 -0.003962291980165844 1107 2 0.020618648821454144 1107 3 0.05343926270662536 1107 4 -0.017743340495925963 1107 5 0.013754823857894394 1107 6 -0.032468147355558585 1107 7 0.02687934800156455 1107 8 0.05005962966586708 1107 9 -0.04525626920884207 1107 10-11 -0.06387707181597335 1107 12-13 -0.07657671702863666 1107 14-15 -0.056984132084203054 1107 16-17 -0.01328766032899864 1107 18-19 -0.05934076533372945 1107 20-21 -0.038824956262963894 1107 22-23 0.016199270187584602 1107 24-25 -0.014157295094264555 1107 26-27 -0.042990269494723066 1107 28-29 -0.07934530108035887 1107 30-31 -0.10975114475559877 1107 32-33 -0.0039286646250928925 1107 34-35 -0.12208463045273987 1107 36-37 -0.02690554430228076 1107 38-39 -0.09397720206004578 1107 40-41 -0.18297405544161194 1107 42-43 -0.09012903781114545 1107 44-45 -0.22022223535373797 1107 46-47 -0.0825914981011664 1107 48-49 -0.21671407535088605 1107 50-51 -0.157242712926994 1107 52-53 -0.1851077613966936 1107 54-55 -0.18347489692237673 1107 56-57 -0.10950125937610267 1107 58-59 -0.1273317334798847 1107 60-61 -0.10608612823391894 1107 62-63 -0.10921712215755264 1107 64-65 -0.14971514438984457 1107 66-67 -0.13129880966266683 1107 68-69 -0.20046460085356443 1107 70-71 -0.1177584374719487 1107 72-73 -0.20909104445364335 1107 74-75 -0.09102511579491335 1107 76-77 -0.05946444588159494 1107 78-79 -0.09677825956240582 1107 80-81 -0.2377691874153811 1107 82-83 -0.1986926970352556 1107 84-85 -0.05618045186560039 1107 86-87 -0.14060855722760834 1107 88-89 -0.12024363798943227 1107 90-91 -0.09150852306946788 1107 92-93 6.894316487482399E-4 1107 94-95 -0.09128958644786067 1107 96-97 -0.12427094770658087 1107 98-99 -0.09399276586005101 1108 1 -0.07059606852012479 1108 2 -0.0015110247812017974 1108 3 -0.013056759041234045 1108 4 -8.358713688636499E-4 1108 5 0.04631238342648203 1108 6 -0.02229098270103691 1108 7 -0.08166735245163181 1108 8 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-0.21193404338352906 2214 42-43 -0.09482315317296042 2214 44-45 -0.18585032169559668 2214 46-47 -0.18917309070122457 2214 48-49 -0.20924842753282036 2214 50-51 -0.12527712094304633 2214 52-53 -0.14475911222602633 2214 54-55 -0.08706621161339712 2214 56-57 -0.18135449960659145 2214 58-59 -0.11302550763640085 2214 60-61 -0.12979289902074242 2214 62-63 -0.18131816070697226 2214 64-65 -0.1496220706197846 2214 66-67 -0.08377730135676131 2214 68-69 -0.13414248798612505 2214 70-71 -0.24509527072865467 2214 72-73 -0.148224232330449 2214 74-75 -0.21999995758326207 2214 76-77 -0.12689363607590565 2214 78-79 -0.10174274448351639 2214 80-81 -0.13107358687175008 2214 82-83 -0.06790001564549186 2214 84-85 -0.07403818559832587 2214 86-87 -0.1617118991445814 2214 88-89 -0.12814608566804964 2214 90-91 -0.22229598902499959 2214 92-93 -0.09267290403827388 2214 94-95 -0.155706660551008 2214 96-97 -0.20656705914256435 2214 98-99 -0.2625511308863224 2215 1 -0.013795073688768866 2215 2 -0.09126947901393834 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-0.12066081695371622 2215 62-63 -0.14926365840384648 2215 64-65 -0.11799303532287553 2215 66-67 -0.13524301109221426 2215 68-69 -0.10312254194079884 2215 70-71 -0.23289474125136422 2215 72-73 -0.16326382228317726 2215 74-75 -0.2029022261486304 2215 76-77 -0.15709414230764196 2215 78-79 -0.139341271290931 2215 80-81 -0.14454521515401098 2215 82-83 -0.11389483204931139 2215 84-85 -0.1302258391011435 2215 86-87 -0.13055916014183566 2215 88-89 -0.03266737221618854 2215 90-91 -0.20569934733445194 2215 92-93 -0.09444757736352827 2215 94-95 -0.07701630353021827 2215 96-97 -0.059334965403877504 2215 98-99 -0.12938016161016108 2216 1 -0.030780303515051344 2216 2 -0.08835967097328279 2216 3 -0.10656605647665884 2216 4 -0.16682099384041038 2216 5 -0.08688091608075155 2216 6 -0.16036431695287945 2216 7 -0.11312013409241928 2216 8 -0.16864934053503333 2216 9 -0.07762490756798712 2216 10-11 -0.08273480948984258 2216 12-13 -0.0077743863691353 2216 14-15 -0.22180621419551727 2216 16-17 -0.2379415528048483 2216 18-19 -0.14188934855854995 2216 20-21 -0.21172407119318137 2216 22-23 -0.2658790626166265 2216 24-25 -0.2564370026710634 2216 26-27 -0.29175664689874026 2216 28-29 -0.16270451712176737 2216 30-31 -0.23067199951209005 2216 32-33 -0.18689561150593192 2216 34-35 -0.23021831696958373 2216 36-37 -0.1949720397256698 2216 38-39 -0.2397867070229509 2216 40-41 -0.16813262054246536 2216 42-43 -0.1645620612297023 2216 44-45 -0.2513225191305182 2216 46-47 -0.24191736099508887 2216 48-49 -0.06899245656625652 2216 50-51 -0.16087844407360308 2216 52-53 -0.14986418483940156 2216 54-55 -0.09913203036339979 2216 56-57 -0.18535932455340287 2216 58-59 -0.22531174890166739 2216 60-61 -0.2390716723367774 2216 62-63 -0.11003032733227514 2216 64-65 -0.20554265517279902 2216 66-67 -0.21044532733541388 2216 68-69 -0.04951760214640899 2216 70-71 -0.13438112219508014 2216 72-73 -0.13736914569754077 2216 74-75 -0.13455947473784846 2216 76-77 -0.15469068495113447 2216 78-79 -0.03182100329385662 2216 80-81 -0.1965350627115825 2216 82-83 -0.13279262858098662 2216 84-85 -0.11964091166447588 2216 86-87 -0.1850442821373619 2216 88-89 -0.1600456873037004 2216 90-91 -0.15556755240369213 2216 92-93 -0.018120245171029836 2216 94-95 -0.15129214438155358 2216 96-97 -0.12844099438543566 2216 98-99 -0.1878974069985766 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 23.0 10 58.0 11 109.0 12 229.0 13 515.0 14 1270.0 15 3341.0 16 7323.0 17 13948.0 18 23182.0 19 32480.0 20 42423.0 21 54519.0 22 66801.0 23 65216.0 24 56741.0 25 55356.0 26 65001.0 27 83142.0 28 108859.0 29 141815.0 30 185386.0 31 243057.0 32 317440.0 33 416074.0 34 553079.0 35 792033.0 36 1331103.0 37 1988835.0 38 696246.0 39 704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.791125024039204 18.78885430632788 12.991278330172598 25.428742339460314 2 16.159130752836358 20.9287627490859 37.05145344377607 25.860653054301675 3 18.054217960551902 25.961465557338915 29.557869248933237 26.426447233175942 4 13.161490168330744 17.27081796564289 36.3169487379448 33.25074312808156 5 14.169442232719012 36.94848684628766 33.32885921206368 15.553211708929643 6 31.682786094952963 36.90325661410196 17.15716908799532 14.256788202949751 7 27.211821007850062 31.682105481240857 22.414978075390493 18.69109543551859 8 27.463185264005297 32.04948715076179 20.033592370374738 20.453735214858177 9 27.403318481888395 13.811326528375941 19.875812499634172 38.909542490101494 10-11 25.73279976848244 24.787903751964762 26.990662388238125 22.488634091314673 12-13 26.966112651642437 22.843233835322245 26.86632106917336 23.324332443861962 14-15 24.436943973112648 23.21820586275499 25.300097124978134 27.04475303915423 16-17 23.514812718678648 25.669334910615927 26.167628702413943 24.64822366829148 18-19 23.9599921293225 24.637834150722657 28.589351212920818 22.812822507034028 20-21 24.934021172030096 23.989334216862485 27.738277902224468 23.33836670888295 22-23 24.322350360609562 23.775443212246255 27.569803402006805 24.332403025137378 24-25 23.927260906868767 24.422101106255916 27.614104548527823 24.03653343834749 26-27 24.138707168808942 24.108447083168684 27.572566693677956 24.18027905434442 28-29 23.72524795097839 24.13634543922793 28.12346904453133 24.014937565262347 30-31 24.347684518938262 23.961346758337402 27.776523176590313 23.914445546134022 32-33 23.862874849703477 24.196831579822852 27.346398757144915 24.593894813328756 34-35 24.31065061089845 24.184539696182206 27.855926600439325 23.648883092480023 36-37 24.437775640045732 24.02006939265163 27.371996638857244 24.170158328445392 38-39 24.455457984286262 23.68091957990888 27.504423648821835 24.35919878698302 40-41 24.277981152717327 23.93660573313599 27.57891681961191 24.20649629453477 42-43 24.99240775404145 23.83372416441395 27.096143901340415 24.07772418020419 44-45 24.485017037803054 24.241214399989328 26.676790568763014 24.596977993444604 46-47 24.350629860347595 24.113299858936003 26.87096966082705 24.66510061988936 48-49 25.12425624235075 24.4453576767986 26.040062828812992 24.390323252037653 50-51 24.253179589048163 24.874770480040933 25.73030191615901 25.14174801475189 52-53 24.198648818434176 24.816448691050518 25.508210855761483 25.476691634753823 54-55 24.416601747462092 24.793362273935855 25.48245643289537 25.30757954570668 56-57 24.669681150171836 24.310507682018905 25.370400191334124 25.649410976475128 58-59 24.136539296599242 24.401461413961567 26.04211502101055 25.41988426842864 60-61 24.313747403288534 24.577478410592747 25.786030566906305 25.322743619212417 62-63 23.89063708302031 24.553139664247812 25.64191061336772 25.91431263936417 64-65 23.75638602830727 24.41791533192646 25.768804233852883 26.05689440591339 66-67 24.458336980288475 24.3573202931376 25.629339678105108 25.555003048468816 68-69 24.266260984594854 24.05227603350852 25.712177173005607 25.96928580889102 70-71 23.805866645177424 24.226451888573735 25.78016367670794 26.187517789540898 72-73 24.441695975027468 24.220094956502656 25.714504871901013 25.623704196568863 74-75 24.558603080527188 24.256948133467052 25.633163327290614 25.551285458715146 76-77 24.16980163419647 24.495843248469996 25.61108470704612 25.72327041028741 78-79 24.112321174641657 24.690148800584673 25.49730248872614 25.700227536047528 80-81 23.76579015626826 24.67600909000635 25.752617691917283 25.805583061808107 82-83 23.99570832130065 24.621907094984223 25.394165601108966 25.988218982606163 84-85 24.093847498325683 24.84669111441437 24.927446272126847 26.1320151151331 86-87 23.750692020020566 24.580210820572738 25.23218998762225 26.43690717178445 88-89 23.83647360408413 24.247967136600142 25.384466277390704 26.53109298192502 90-91 23.934792425913557 24.452479553294832 25.19784120635986 26.414886814431753 92-93 23.793970257962567 24.680503250557447 25.03118788669219 26.494338604787792 94-95 23.121072348455122 24.697724268558648 25.545185990939707 26.63601739204652 96-97 23.6921016821633 24.580592419269617 25.118008844205864 26.60929705436122 98-99 23.457778280527897 24.709803035163265 25.13553664480044 26.696882039508402 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 82.0 1 114.0 2 394.5 3 638.0 4 450.0 5 387.0 6 769.0 7 2121.5 8 3954.0 9 3908.5 10 2379.5 11 1555.5 12 1545.0 13 1751.5 14 2109.0 15 2588.5 16 3111.5 17 3822.5 18 4704.0 19 5653.0 20 6980.5 21 8643.0 22 10753.5 23 13159.5 24 15579.5 25 18819.0 26 23505.5 27 28266.5 28 34423.0 29 43030.5 30 50585.0 31 59861.5 32 70925.0 33 79909.5 34 87651.5 35 97961.5 36 105938.5 37 117627.5 38 132261.5 39 130144.5 40 128891.0 41 137982.5 42 149507.5 43 170662.5 44 195482.0 45 221506.5 46 257213.0 47 292678.0 48 316309.0 49 337001.0 50 362770.0 51 353727.5 52 330960.0 53 328611.5 54 314526.0 55 306758.0 56 292799.0 57 268055.5 58 255177.0 59 230780.5 60 192166.5 61 150774.5 62 123106.5 63 99599.0 64 74035.0 65 57120.5 66 42473.5 67 31833.0 68 24680.0 69 20792.5 70 18519.5 71 16763.5 72 16668.0 73 14096.5 74 9543.0 75 7512.0 76 5881.5 77 4009.0 78 2441.5 79 1645.5 80 1130.5 81 771.5 82 501.0 83 322.0 84 214.0 85 143.5 86 75.5 87 30.5 88 17.0 89 11.0 90 4.5 91 3.0 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056341203088189434 2 0.0 3 0.0 4 0.0 5 0.004927643275652229 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014429010696661222 16-17 0.004675816202172764 18-19 7.690934946805274E-4 20-21 2.0418411363199842E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.5863321060053135E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.3612274242133229E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 4.7642959847466295E-5 76-77 1.2931660530026567E-4 78-79 3.403068560533307E-5 80-81 2.041841136319984E-5 82-83 2.041841136319984E-5 84-85 4.2198050150613005E-4 86-87 8.711855514965266E-4 88-89 3.1308230756906425E-4 90-91 0.0010685635280074583 92-93 0.0014905440295135885 94-95 0.0010821758022495917 96-97 0.001980585902230385 98-99 6.874198492277281E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7346311.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.7801475945501 #Duplication Level Percentage of deduplicated Percentage of total 1 71.75393127300686 25.673662514373895 2 15.172807198676285 10.857705619845795 3 5.31609275330825 5.706317500190621 4 2.4197429007334663 3.4631503251643285 5 1.2875661941112218 2.3034654231526326 6 0.7823230044875925 1.6794979540306767 7 0.5168834169726599 1.2945915463920008 8 0.3870501888542243 1.1078970306962108 9 0.2916389432167336 0.9391395989352008 >10 1.7077407759334842 11.4720092705788 >50 0.16204709668433923 4.053258105256973 >100 0.15715035580110454 12.056558726830106 >500 0.028179277617714257 6.939587019954701 >1k 0.01623816477315383 10.315840473159266 >5k 4.1831337818911595E-4 0.95907829424647 >10k+ 1.9014244463141634E-4 1.1782405971921313 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 21732 0.2958219438300393 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20755 0.28252275189547515 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 20512 0.2792149692546368 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12838 0.1747543767205064 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10756 0.14641362174838501 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 7955 0.10828564159616984 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.3612274242133229E-5 4.083682272639968E-5 2 0.0 0.0 0.0 1.3612274242133229E-5 4.083682272639968E-5 3 0.0 0.0 0.0 1.3612274242133229E-5 4.083682272639968E-5 4 0.0 0.0 0.0 1.3612274242133229E-5 4.083682272639968E-5 5 0.0 1.3612274242133229E-5 0.0 2.7224548484266457E-5 4.083682272639968E-5 6 0.0 1.3612274242133229E-5 0.0 2.7224548484266457E-5 4.083682272639968E-5 7 0.0 1.3612274242133229E-5 0.0 2.7224548484266457E-5 4.083682272639968E-5 8 0.0 1.3612274242133229E-5 0.0 2.7224548484266457E-5 4.083682272639968E-5 9 0.0 1.3612274242133229E-5 0.0 4.083682272639968E-5 4.083682272639968E-5 10-11 0.0 1.3612274242133229E-5 0.0 1.0209205681599922E-4 4.083682272639968E-5 12-13 0.0 1.3612274242133229E-5 0.0 1.0889819393706583E-4 2.1779638787413166E-4 14-15 0.0 1.3612274242133229E-5 0.0 1.1570433105813245E-4 3.1988844469013085E-4 16-17 0.0 1.3612274242133229E-5 0.0 1.2251046817919907E-4 3.947559530218636E-4 18-19 0.0 2.0418411363199845E-5 0.0 1.7695956514773197E-4 4.628173242325298E-4 20-21 0.0 6.806137121066614E-5 0.0 3.0627617044799764E-4 5.104602840799961E-4 22-23 0.0 7.486750833173275E-5 0.0 0.0011774617219445242 5.376848325642625E-4 24-25 0.0 8.167364545279936E-5 0.0 0.003403068560533307 5.444909696853291E-4 26-27 2.7224548484266457E-5 8.167364545279936E-5 0.0 0.005941757706691154 5.989400666538621E-4 28-29 2.7224548484266457E-5 8.167364545279936E-5 0.0 0.017022148939787603 6.329707522591951E-4 30-31 2.7224548484266457E-5 1.0889819393706583E-4 0.0 0.050134006033776685 6.397768893802617E-4 32-33 2.7224548484266457E-5 1.0889819393706583E-4 0.0 0.11058611594309034 6.397768893802617E-4 34-35 2.7224548484266457E-5 1.1570433105813245E-4 0.0 0.18790383363840707 6.942259863487947E-4 36-37 2.7224548484266457E-5 1.2251046817919907E-4 0.0 0.30802534768811174 6.942259863487947E-4 38-39 2.7224548484266457E-5 1.2251046817919907E-4 0.0 0.4940642997553466 7.214505348330611E-4 40-41 3.403068560533307E-5 1.2251046817919907E-4 0.0 0.7255342171057011 7.486750833173276E-4 42-43 4.083682272639968E-5 1.2251046817919907E-4 0.0 0.932011998947499 7.827057689226606E-4 44-45 4.083682272639968E-5 1.2251046817919907E-4 0.0 1.1639991827190546 8.439610030122602E-4 46-47 4.083682272639968E-5 1.3612274242133228E-4 0.0 1.4241025189377363 8.711855514965266E-4 48-49 4.083682272639968E-5 1.3612274242133228E-4 0.0 1.6954087568576937 9.052162371018597E-4 50-51 4.083682272639968E-5 1.4973501666346552E-4 0.0 1.9943615237634238 9.120223742229263E-4 52-53 4.083682272639968E-5 1.6334729090559873E-4 0.0 2.2946755180933667 9.936960196757258E-4 54-55 4.083682272639968E-5 2.3140866211626487E-4 0.0 2.588625774215113 0.0010209205681599922 56-57 4.083682272639968E-5 2.7224548484266456E-4 0.0 2.9220039826791977 0.0010345328424021254 58-59 4.083682272639968E-5 2.9947003332693104E-4 0.0 3.286091481833535 0.0010549512537653253 60-61 4.083682272639968E-5 3.1308230756906425E-4 0.0 3.653874713444612 0.0010617573908863918 62-63 4.083682272639968E-5 3.2669458181119746E-4 0.0 4.022923886560207 0.0011162064878549247 64-65 4.083682272639968E-5 3.2669458181119746E-4 0.0 4.409750689836027 0.0011366248992181246 66-67 4.083682272639968E-5 3.87949815900797E-4 0.0 4.828457439386925 0.001163849447702391 68-69 4.083682272639968E-5 3.947559530218636E-4 0.0 5.282658194024185 0.001184267859065591 70-71 4.083682272639968E-5 4.083682272639969E-4 0.0 5.796242495042749 0.0012046862704287909 72-73 4.083682272639968E-5 4.287866386271967E-4 0.0 6.286767603495141 0.0012251046817919906 74-75 4.083682272639968E-5 4.492050499903965E-4 0.0 6.773515305845342 0.001252329230276257 76-77 4.083682272639968E-5 4.492050499903965E-4 0.0 7.292156566744859 0.0012863599158815901 78-79 4.083682272639968E-5 4.492050499903965E-4 0.0 7.829746113389428 0.0013135844643658566 80-81 4.083682272639968E-5 4.5601118711146313E-4 0.0 8.421253878307084 0.001340809012850123 82-83 4.083682272639968E-5 4.628173242325298E-4 0.0 9.046833165652803 0.0014224826583029222 84-85 4.083682272639968E-5 4.628173242325298E-4 0.0 9.657622717034442 0.001429288795423989 86-87 4.083682272639968E-5 4.8323573559572966E-4 0.0 10.280765407290815 0.0014497072067871887 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 52795 0.0 25.981203 1 TATCACG 560 0.0 25.74157 2 GTACTAG 2865 0.0 23.06154 1 AGTACTT 31495 0.0 22.821543 12-13 CAACGCA 62565 0.0 21.561438 5 ATCAACG 63300 0.0 21.32577 3 AACGCAG 65430 0.0 20.921925 6 TATCAAC 65700 0.0 20.879402 2 TCAACGC 65725 0.0 20.560156 4 TACTTTT 35895 0.0 20.095617 14-15 GAGTACT 29990 0.0 19.702248 12-13 ACGCAGA 69545 0.0 19.456638 7 CGCAGAG 69915 0.0 19.33372 8 GCAGAGT 73130 0.0 18.408329 9 CATGGGG 15735 0.0 18.145254 4 TAGGCAT 4395 0.0 17.880896 5 TATTCGC 525 0.0 17.71463 9 ATACGGG 2420 0.0 17.485878 3 GTACTTT 34025 0.0 17.372845 14-15 CTATTGA 3490 0.0 17.321255 9 >>END_MODULE