FastQCFastQC Report
Fri 27 May 2016
ERR765327_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765327_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6667920
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1392832.0888522957683957No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1168211.75198562670218No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG712081.0679192311845374No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT579080.8684567301347347No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG553070.8294490635760478No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG534810.8020642119281576No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA523950.785777273872512No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC522730.7839476178478445No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC449750.6744981943394641No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT437850.6566515495086924No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC380220.5702227981139546No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC342610.5138184021403976No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG238190.35721784304550747No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA230530.34573000275948124No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG225530.33823141249445104No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC223140.33464708634776663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214720.3220194603414558No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC191440.2871060240674753No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC174140.2611609017504709No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT173810.2606659947929789No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC172880.2592712570036833No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA157920.23683547493071302No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG150630.22590253032429902No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG147630.2214033761652809No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC147550.22128339872104047No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT147110.22062352277771777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144450.21663427275672173No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA138250.20733602082808433No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA134750.2020870076425632No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG131560.19730290705347395No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA122320.18344551224369818No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA120580.18083600283146767No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC118070.17707171051842255No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC114900.1723176042903934No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA105180.15774034481517474No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG104180.1562406267621687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102110.1531362103924462No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG101260.1518614500473911No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC100080.15009178274484397No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG94890.14230824604974265No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC93700.14052358156666545No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA92760.13911384659683979No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT85550.12830087943466628No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA84790.1271610937143817No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT82750.12410166888624939No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG81330.12197206925098081No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT80740.12108723559970726No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC78510.1177428643415038No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG75280.1128987750302943No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA67810.10169588117433923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC85950.031.2779051
ATCTACA97450.027.5155092
TCTACAC114500.025.3459593
TATCACG3750.025.0889952
TACACTC118750.024.2426175
AGTACTC34050.023.7614565
CTACACT130450.022.8987054
CACTCTT140000.020.715977
ACACTCT142650.020.2980256
GTATAAG15300.019.6940921
AAGTACT42900.019.2990844
ACTCTTT159100.019.1448948
AAAGTAC42100.018.0993063
AGTACTT298500.017.74404312-13
GTATAAT13150.017.5435621
GTACTAG14000.016.814711
GTGATCG10450.016.6430748
GAGTACT293800.016.63621712-13
GTATTAG20150.016.58941
ATGGGTA42600.016.2319035
Produced by FastQC (version 0.11.5)