##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765327_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6667920 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29731475482609 33.0 31.0 34.0 31.0 34.0 2 32.31300585489928 34.0 31.0 34.0 31.0 34.0 3 32.34914216127368 34.0 31.0 34.0 31.0 34.0 4 35.80444651405536 37.0 35.0 37.0 35.0 37.0 5 35.815306272420784 37.0 35.0 37.0 35.0 37.0 6 35.93019037420965 37.0 37.0 37.0 35.0 37.0 7 35.883032939807315 37.0 37.0 37.0 35.0 37.0 8 35.923235581710635 37.0 37.0 37.0 35.0 37.0 9 37.625130175527 39.0 38.0 39.0 35.0 39.0 10-11 37.55588699324527 39.0 38.0 39.0 35.0 39.0 12-13 37.5356184537307 39.0 38.0 39.0 35.0 39.0 14-15 38.902664474078875 41.0 39.0 41.0 36.0 41.0 16-17 38.84154683919424 40.5 39.0 41.0 35.5 41.0 18-19 38.829869134602696 41.0 39.0 41.0 35.0 41.0 20-21 38.81620715305523 41.0 39.0 41.0 35.0 41.0 22-23 38.747334101188976 41.0 39.0 41.0 35.0 41.0 24-25 38.676590900910625 41.0 39.0 41.0 35.0 41.0 26-27 38.5299711154303 40.0 38.0 41.0 34.0 41.0 28-29 38.371190266229945 40.0 38.0 41.0 34.0 41.0 30-31 38.12009142281251 40.0 38.0 41.0 34.0 41.0 32-33 37.80861400556695 40.0 37.0 41.0 33.0 41.0 34-35 37.696323741136666 40.0 38.0 41.0 33.0 41.0 36-37 37.56600071686523 40.0 37.5 41.0 33.0 41.0 38-39 37.36199864425488 40.0 37.0 41.0 33.0 41.0 40-41 37.095347649641866 40.0 37.0 41.0 32.0 41.0 42-43 36.81792448019772 40.0 36.5 41.0 31.0 41.0 44-45 36.40077640403604 40.0 36.0 41.0 30.0 41.0 46-47 35.97057920610925 39.0 35.0 41.0 29.5 41.0 48-49 35.59838060444636 39.0 35.0 41.0 27.5 41.0 50-51 34.361294376657185 38.0 34.0 40.0 25.5 40.5 52-53 34.31201124188652 38.0 34.5 39.5 24.5 40.0 54-55 35.109206769127404 39.0 35.0 40.5 26.0 41.0 56-57 35.10034103588525 39.0 35.0 41.0 26.0 41.0 58-59 34.89241345127117 39.0 35.0 41.0 26.0 41.0 60-61 34.49658446712018 38.0 35.0 40.5 25.0 41.0 62-63 34.07218705383388 37.0 34.0 40.0 23.0 41.0 64-65 33.81230706127248 37.0 34.0 40.0 22.0 41.0 66-67 33.46382867520906 36.0 34.0 39.0 22.0 41.0 68-69 33.202187338780305 36.0 34.0 39.0 21.5 41.0 70-71 32.79738741616576 35.0 34.0 38.5 20.0 40.5 72-73 32.35810852559719 35.0 34.0 37.0 19.0 39.5 74-75 31.961286878066925 35.0 33.0 37.0 16.5 39.0 76-77 31.55732462297088 35.0 33.0 36.0 11.5 38.5 78-79 31.19985227777178 35.0 33.0 36.0 6.5 37.0 80-81 30.85439657344419 35.0 33.0 35.5 2.0 37.0 82-83 30.55572059952729 35.0 32.5 35.0 2.0 36.5 84-85 30.257156654548943 35.0 32.0 35.0 2.0 36.0 86-87 29.97986448547673 35.0 32.0 35.0 2.0 36.0 88-89 29.71336068819062 35.0 31.5 35.0 2.0 35.5 90-91 29.44396903382164 35.0 31.0 35.0 2.0 35.0 92-93 29.113386183397523 35.0 31.0 35.0 2.0 35.0 94-95 28.757091266841833 35.0 31.0 35.0 2.0 35.0 96-97 28.408565564673843 35.0 30.0 35.0 2.0 35.0 98-99 28.03975842541602 35.0 29.5 35.0 2.0 35.0 100 27.43142659180074 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4980106663447259 1101 2 0.5343482147341732 1101 3 0.5450784478948236 1101 4 0.4691678850638539 1101 5 0.5045828401683252 1101 6 0.4450786164811902 1101 7 0.4501456881896502 1101 8 0.33658126292617396 1101 9 0.4832626889200995 1101 10-11 0.4345345570094068 1101 12-13 0.49019988316776164 1101 14-15 0.6273649730824076 1101 16-17 0.5743286039169817 1101 18-19 0.5455302829988327 1101 20-21 0.4908773206336434 1101 22-23 0.5557356232409703 1101 24-25 0.48079310308839496 1101 26-27 0.5504087567103397 1101 28-29 0.5080910976644333 1101 30-31 0.5833387995289954 1101 32-33 0.5298669698673066 1101 34-35 0.6171006399888768 1101 36-37 0.5713521510405428 1101 38-39 0.6074007830062698 1101 40-41 0.6594478792151435 1101 42-43 0.6435643527188617 1101 44-45 0.6922858670738563 1101 46-47 0.7404359408336134 1101 48-49 0.7268383659588409 1101 50-51 0.7488194888668502 1101 52-53 0.8049492544942041 1101 54-55 0.8206732476053205 1101 56-57 0.920793543876762 1101 58-59 0.8887896243844651 1101 60-61 0.943509193610538 1101 62-63 0.9871167596743433 1101 64-65 0.9467857550451058 1101 66-67 0.9228089567667084 1101 68-69 0.9242491064284835 1101 70-71 0.8532268942291097 1101 72-73 0.8088514038803183 1101 74-75 0.7385598483006248 1101 76-77 0.8068074297980594 1101 78-79 0.7822650938812181 1101 80-81 0.8561704086919306 1101 82-83 0.8348288251040223 1101 84-85 0.7877622359909218 1101 86-87 0.7882708552928754 1101 88-89 0.8049370239673657 1101 90-91 0.8707676468220598 1101 92-93 0.8450544313450656 1101 94-95 0.8026345875477752 1101 96-97 0.828813983494662 1101 98-99 0.875118880014341 1101 100 0.8623615069225856 1102 1 0.482171580720383 1102 2 0.5609234268870793 1102 3 0.5599186028108747 1102 4 0.4901810429100948 1102 5 0.4749366390646159 1102 6 0.45479851349765 1102 7 0.47153652405054913 1102 8 0.44743338921616527 1102 9 0.4085388511609196 1102 10-11 0.5487806145932126 1102 12-13 0.5024697366852493 1102 14-15 0.6618492547499599 1102 16-17 0.6949737710787005 1102 18-19 0.6676367666130716 1102 20-21 0.6218286074277302 1102 22-23 0.6164772703443191 1102 24-25 0.5882291668975128 1102 26-27 0.5992663324243779 1102 28-29 0.6069517871631618 1102 30-31 0.5450222499213311 1102 32-33 0.6309579076482592 1102 34-35 0.5869210904391977 1102 36-37 0.5836475390967166 1102 38-39 0.6289728426402093 1102 40-41 0.609905120445525 1102 42-43 0.5468567814009688 1102 44-45 0.4883431237077147 1102 46-47 0.4716099534874658 1102 48-49 0.4154053307942078 1102 50-51 0.41726234155391495 1102 52-53 0.42734314269144136 1102 54-55 0.5425455054158661 1102 56-57 0.5196619802311133 1102 58-59 0.5125575828932085 1102 60-61 0.475049500914011 1102 62-63 0.4737845151628193 1102 64-65 0.41874102831092586 1102 66-67 0.32724167746916777 1102 68-69 0.3454978250948102 1102 70-71 0.3070122936927149 1102 72-73 0.3251882625845184 1102 74-75 0.35991667320897847 1102 76-77 0.35139581115424434 1102 78-79 0.2838632213082697 1102 80-81 0.3041623071356163 1102 82-83 0.4059402401559531 1102 84-85 0.38256579190294815 1102 86-87 0.34447846711253405 1102 88-89 0.30157906290366654 1102 90-91 0.3392279036100163 1102 92-93 0.3475126289736714 1102 94-95 0.35831770715689615 1102 96-97 0.3068177271062318 1102 98-99 0.3224777550565818 1102 100 0.4060517795118699 1103 1 0.5237941980144996 1103 2 0.5420754016779483 1103 3 0.5883473643617663 1103 4 0.42742127445366407 1103 5 0.4896307499390957 1103 6 0.4797048075138264 1103 7 0.454424259749338 1103 8 0.4283129882358949 1103 9 0.5116139802698001 1103 10-11 0.5561331497589777 1103 12-13 0.5419805576434698 1103 14-15 0.6826585675853494 1103 16-17 0.6128169191193678 1103 18-19 0.6378126701003808 1103 20-21 0.6881889076664365 1103 22-23 0.7181202295111646 1103 24-25 0.6911434405889096 1103 26-27 0.6427214549708964 1103 28-29 0.6161593767517317 1103 30-31 0.6391206558315403 1103 32-33 0.6978998819450695 1103 34-35 0.701259600196039 1103 36-37 0.6619388364930003 1103 38-39 0.7393120084238447 1103 40-41 0.7133065822690341 1103 42-43 0.6097583225142174 1103 44-45 0.7006726074237903 1103 46-47 0.5820048094194448 1103 48-49 0.5654107107321451 1103 50-51 0.5780458748051416 1103 52-53 0.5791475542685589 1103 54-55 0.6813227640452837 1103 56-57 0.6917374482428258 1103 58-59 0.6406184883425396 1103 60-61 0.6306143367992902 1103 62-63 0.5930636567004939 1103 64-65 0.5897279386225449 1103 66-67 0.5493931456390087 1103 68-69 0.5185522737432535 1103 70-71 0.5020994352068584 1103 72-73 0.4658214684998683 1103 74-75 0.4966631584533232 1103 76-77 0.46291889591997304 1103 78-79 0.5188991243845145 1103 80-81 0.4658437280442769 1103 82-83 0.4539486144416536 1103 84-85 0.5034334244560945 1103 86-87 0.5192515371985422 1103 88-89 0.5025479314279586 1103 90-91 0.45149197722479784 1103 92-93 0.563093499980198 1103 94-95 0.4916189137580993 1103 96-97 0.5962512803908488 1103 98-99 0.5210248636874546 1103 100 0.4989633252438459 1104 1 0.411920045911522 1104 2 0.46054678555040596 1104 3 0.49481333841605846 1104 4 0.42952763677866557 1104 5 0.4246878477687872 1104 6 0.43136607867316457 1104 7 0.396319397328746 1104 8 0.3800723770698724 1104 9 0.4584145513526181 1104 10-11 0.5277799557167242 1104 12-13 0.47198063964938797 1104 14-15 0.5777851485510013 1104 16-17 0.6143187048385315 1104 18-19 0.6049306426952867 1104 20-21 0.6312172223820127 1104 22-23 0.5912925707464964 1104 24-25 0.6032369665750608 1104 26-27 0.5948998297517889 1104 28-29 0.6288421876510597 1104 30-31 0.6599264428126261 1104 32-33 0.7119579964874063 1104 34-35 0.6301315540578969 1104 36-37 0.6466424485966229 1104 38-39 0.7167777938645159 1104 40-41 0.7053147031065308 1104 42-43 0.662006273692711 1104 44-45 0.6491039868775843 1104 46-47 0.6501922839634418 1104 48-49 0.6405817131805875 1104 50-51 0.5910571917832783 1104 52-53 0.643166812475144 1104 54-55 0.7125719088119524 1104 56-57 0.6908611104408067 1104 58-59 0.6484328327220723 1104 60-61 0.62870178180507 1104 62-63 0.6373434782749001 1104 64-65 0.6232242386278699 1104 66-67 0.5715871320964467 1104 68-69 0.6018340317830138 1104 70-71 0.6019576292788642 1104 72-73 0.5846711087109355 1104 74-75 0.5676872821838614 1104 76-77 0.5676427586626396 1104 78-79 0.5122515474102975 1104 80-81 0.5863757921188828 1104 82-83 0.6685108885334223 1104 84-85 0.6372278722692108 1104 86-87 0.5957900446875328 1104 88-89 0.6532384132577782 1104 90-91 0.5596686591461726 1104 92-93 0.6009079263656538 1104 94-95 0.43071491584072774 1104 96-97 0.5092733546165 1104 98-99 0.49290133446769957 1104 100 0.4952849576183702 1105 1 0.42223770963369844 1105 2 0.4184478541261427 1105 3 0.42108989235457983 1105 4 0.4198349700445263 1105 5 0.4565874031385846 1105 6 0.42355567540422356 1105 7 0.4178900735530604 1105 8 0.3892286311360067 1105 9 0.41890611985147075 1105 10-11 0.4316342498438317 1105 12-13 0.4289227457218914 1105 14-15 0.5810960203705378 1105 16-17 0.5767917670127218 1105 18-19 0.5875935259089928 1105 20-21 0.5418390606512204 1105 22-23 0.5061087582823873 1105 24-25 0.5798761598356421 1105 26-27 0.5464040326878603 1105 28-29 0.6010358674971599 1105 30-31 0.5757255570570479 1105 32-33 0.7059241863671488 1105 34-35 0.7377497179616768 1105 36-37 0.7663628866465189 1105 38-39 0.7189678243170192 1105 40-41 0.7435036575793603 1105 42-43 0.7177063592541231 1105 44-45 0.7694838129596562 1105 46-47 0.6770087991999887 1105 48-49 0.6793768262351207 1105 50-51 0.6451847961381887 1105 52-53 0.6873704319901535 1105 54-55 0.7273875268146313 1105 56-57 0.7123988851693923 1105 58-59 0.7597341679269789 1105 60-61 0.7401535613992181 1105 62-63 0.6743800041923862 1105 64-65 0.7033894558325002 1105 66-67 0.6825991882384059 1105 68-69 0.5784507503867218 1105 70-71 0.5857542778825646 1105 72-73 0.6196703266980279 1105 74-75 0.6824933495526366 1105 76-77 0.6663677928455876 1105 78-79 0.6697068467197163 1105 80-81 0.7420837635248105 1105 82-83 0.7206557725260758 1105 84-85 0.7433208700335747 1105 86-87 0.7538118104945966 1105 88-89 0.7274478463926108 1105 90-91 0.780417455131051 1105 92-93 0.7878126929643692 1105 94-95 0.8413622078636287 1105 96-97 0.8081126459244139 1105 98-99 0.7930775392823612 1105 100 0.8241237976807376 1106 1 0.3016888607808852 1106 2 0.32374032520687024 1106 3 0.31626767101297304 1106 4 0.3297737391093989 1106 5 0.2599289462285981 1106 6 0.2996478312238793 1106 7 0.3221689539039616 1106 8 0.3485322548369467 1106 9 0.359778499564122 1106 10-11 0.32768680189332855 1106 12-13 0.437148033293532 1106 14-15 0.525500676915712 1106 16-17 0.4937468158929974 1106 18-19 0.4774326410965699 1106 20-21 0.4813308322814933 1106 22-23 0.5065490269988189 1106 24-25 0.5294597678615887 1106 26-27 0.5060059055210928 1106 28-29 0.4958915402949273 1106 30-31 0.5491375935770577 1106 32-33 0.5434584654036101 1106 34-35 0.48856354861973017 1106 36-37 0.5669707528823267 1106 38-39 0.5589626286738323 1106 40-41 0.65022869317432 1106 42-43 0.6503179865271633 1106 44-45 0.63890553146868 1106 46-47 0.6568936944885664 1106 48-49 0.6405571658892839 1106 50-51 0.5794910116565077 1106 52-53 0.6353614032592105 1106 54-55 0.6617514628065564 1106 56-57 0.6875453378620193 1106 58-59 0.7455362523485789 1106 60-61 0.7090746488657587 1106 62-63 0.6962588656500444 1106 64-65 0.7059486103116299 1106 66-67 0.738933569906834 1106 68-69 0.7069776334371198 1106 70-71 0.6654696543045091 1106 72-73 0.5997381102278325 1106 74-75 0.6618425091833657 1106 76-77 0.6453355650474499 1106 78-79 0.6011763131095478 1106 80-81 0.6309807380833732 1106 82-83 0.6074397454478913 1106 84-85 0.5673543222902957 1106 86-87 0.5847336929185509 1106 88-89 0.6100068567706849 1106 90-91 0.6424842964001485 1106 92-93 0.6784465254076757 1106 94-95 0.6660428739466866 1106 96-97 0.7415889194874374 1106 98-99 0.6853004860126042 1106 100 0.7347129427892902 1107 1 0.1849528683866808 1107 2 0.25668649302508584 1107 3 0.3115569099188775 1107 4 0.2752436503091502 1107 5 0.382373540058488 1107 6 0.2901960856896153 1107 7 0.3233367078196423 1107 8 0.3133861390563055 1107 9 0.36950262620340624 1107 10-11 0.3250295588758618 1107 12-13 0.3012948222979759 1107 14-15 0.3495849491019243 1107 16-17 0.44767805209215084 1107 18-19 0.43717761739275574 1107 20-21 0.4150786978504257 1107 22-23 0.38944929094607517 1107 24-25 0.3936186511509945 1107 26-27 0.42284675215071843 1107 28-29 0.479396506124246 1107 30-31 0.39227534822482113 1107 32-33 0.41176233715768973 1107 34-35 0.408507259492616 1107 36-37 0.4760143843138138 1107 38-39 0.44296291606767824 1107 40-41 0.4881199890485064 1107 42-43 0.49923280269403847 1107 44-45 0.4761863998432858 1107 46-47 0.45864066325018626 1107 48-49 0.4210316971553638 1107 50-51 0.4085032474124617 1107 52-53 0.3913359305680473 1107 54-55 0.39389108526700056 1107 56-57 0.32726432999504596 1107 58-59 0.2962167228236794 1107 60-61 0.2753411406228494 1107 62-63 0.2911978154802739 1107 64-65 0.32080464627492944 1107 66-67 0.2970663458642875 1107 68-69 0.26904953532155673 1107 70-71 0.248064420076048 1107 72-73 0.2854405631170067 1107 74-75 0.23498372393434153 1107 76-77 0.2808774285863258 1107 78-79 0.23669764851647201 1107 80-81 0.33139362559514396 1107 82-83 0.3058510287974521 1107 84-85 0.23357237507579143 1107 86-87 0.36095266823335237 1107 88-89 0.327911209031452 1107 90-91 0.26539391425042425 1107 92-93 0.3294911476306943 1107 94-95 0.30551131157282185 1107 96-97 0.2309412989699915 1107 98-99 0.3316194951617213 1107 100 0.42331166776970264 1108 1 0.15663922088115356 1108 2 0.1971558888724374 1108 3 0.20929824655254237 1108 4 0.1966425256335782 1108 5 0.1618256812677501 1108 6 0.13274450922128977 1108 7 0.20995144635659813 1108 8 0.22537066275139495 1108 9 0.2680490190708511 1108 10-11 0.2573935704590582 1108 12-13 0.26049829725711504 1108 14-15 0.28497918634118236 1108 16-17 0.23767252484039858 1108 18-19 0.2686880590922698 1108 20-21 0.2948331857385327 1108 22-23 0.25394450867172935 1108 24-25 0.26723533624183204 1108 26-27 0.29340317217379663 1108 28-29 0.328540833481739 1108 30-31 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-1.2592965504895304 2316 60-61 -1.1266602968489252 2316 62-63 -1.2200723127092203 2316 64-65 -1.1032850744277667 2316 66-67 -1.1351297283322026 2316 68-69 -1.2198354128673259 2316 70-71 -1.0152806621693244 2316 72-73 -1.106860665783941 2316 74-75 -1.0993804024221774 2316 76-77 -1.1226990441949134 2316 78-79 -1.0041141974219236 2316 80-81 -0.9422884380312055 2316 82-83 -0.896188604587504 2316 84-85 -0.960107734132631 2316 86-87 -1.0805267417460236 2316 88-89 -1.0662635389330255 2316 90-91 -0.9491496809943314 2316 92-93 -0.9809906750130146 2316 94-95 -0.7972192462630119 2316 96-97 -1.0368689224938628 2316 98-99 -0.846852878913122 2316 100 -0.9969347875420134 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19474.0 3 3035.0 4 3004.0 5 3409.0 6 3957.0 7 4902.0 8 5990.0 9 7627.0 10 10328.0 11 15575.0 12 24709.0 13 35716.0 14 35807.0 15 41460.0 16 53046.0 17 71305.0 18 70941.0 19 42696.0 20 37185.0 21 34721.0 22 35788.0 23 40465.0 24 45935.0 25 47838.0 26 52142.0 27 61175.0 28 72820.0 29 87591.0 30 105677.0 31 129176.0 32 162663.0 33 215445.0 34 309966.0 35 490865.0 36 888229.0 37 1678248.0 38 1447158.0 39 271496.0 40 356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.120167856200965 17.006885634641527 13.290822411027342 23.582124098130166 2 13.901353789758522 22.18492152339062 36.75030295537348 27.16342173147738 3 19.382995131268046 28.304496323369598 29.18021828897817 23.132290256384184 4 13.18112314930071 18.649889249491373 34.0062802495553 34.162707351652614 5 13.043529287853026 36.651675418666585 33.192353410270684 17.1124418832097 6 30.374489640626777 32.80259614181309 18.891060085731812 17.931854131828324 7 24.585092308143086 32.63103564079274 22.135472427013344 20.648399624050832 8 27.996148678436427 30.632186023037335 21.681618576468033 19.690046722058206 9 28.36347902876127 14.84681886944099 19.359084991955235 37.43061710984251 10-11 24.381084673850246 26.38946437170342 25.597716931801294 23.63173402264504 12-13 27.426211498035602 24.072365332624724 26.03813184264817 22.4632913266915 14-15 26.440972368280306 22.468612200454057 25.40622509918262 25.684190332083006 16-17 22.939228922789756 26.003533526175364 28.169540157151257 22.88769739388362 18-19 24.701201803407493 25.080531262566286 29.382993921759244 20.835273012266985 20-21 23.9337633775769 24.696281221672724 28.97110175122935 22.39885364952103 22-23 22.489987201465812 26.531042867953353 25.59807494661769 25.38089498396315 24-25 22.601658025404223 27.400058831462886 24.943766363205015 25.054516779927877 26-27 23.959760664765 24.764442180374495 25.57360925934483 25.702187895515678 28-29 23.249030753950734 23.968939032103023 28.997481857991218 23.784548355955025 30-31 25.46769547855714 24.159867569953303 27.783627419750033 22.588809531739525 32-33 21.024979780341717 25.7545421796615 28.613433880082713 24.607044159914064 34-35 20.624101769783895 26.485797448185032 28.928795878835658 23.961304903195416 36-37 23.34875785616931 26.329395382586217 26.874987467903754 23.44685929334072 38-39 24.49703639151463 24.365574875182507 26.563349270974534 24.57403946232833 40-41 22.78936546122997 26.03013011221623 26.593583388682774 24.586921037871026 42-43 25.33105273779578 24.9561013888989 26.304352591962598 23.408493281342725 44-45 25.03279940192153 24.155625653097065 27.40580008764529 23.405774857336116 46-47 23.187800215545433 24.74781086432848 29.40249071218283 22.661898207943263 48-49 23.736072078029427 25.867536332075925 28.392769653887196 22.003621936007452 50-51 22.273364468071964 26.58063557130612 26.820871948029346 24.32512801259257 52-53 21.609228529822396 27.396869375485107 24.620123718301464 26.373778376391034 54-55 22.66011612031957 26.51013941988473 24.462614611232738 26.36712984856296 56-57 23.72459847156487 24.354248695583493 25.13202585603014 26.789126976821503 58-59 23.545870415637456 24.118651257840217 27.663157945327267 24.67232038119506 60-61 23.7366596669412 25.01107768494028 26.540436365438225 24.711826282680295 62-63 20.734590180873585 25.59138507546612 27.619326824415413 26.054697919244884 64-65 20.833011451486307 25.767552132659617 27.307847798497082 26.091588617356997 66-67 23.307719110393567 25.417624750620384 25.648923803974505 25.62573233501154 68-69 24.107230390041018 24.302230631004477 25.61464072949022 25.975898249464286 70-71 22.71963475681953 25.31193237198502 26.01813286063083 25.950300010564625 72-73 24.479270209595736 24.646228195290824 25.944985578750508 24.929516016362935 74-75 24.777322940472462 23.901551378145015 26.570896127666803 24.75022955371572 76-77 23.424097265979352 25.32025712516327 27.586380707620034 23.669264901237337 78-79 23.85069154354497 25.24748379856383 27.255846503954402 23.645978153936806 80-81 22.85044382307129 25.43494660986732 26.752476213710665 24.962133353350723 82-83 22.857058534540545 26.37729545487656 24.75252335387422 26.013122656708674 84-85 23.282918122733268 26.291402120774155 24.684190046363767 25.741489710128818 86-87 23.718838576023423 24.448501969948417 25.528789961598758 26.303869492429406 88-89 24.084267993914114 23.768187900860564 26.815931926988874 25.33161217823645 90-91 24.75291063768222 24.618959218809028 25.9489633976546 24.67916674585415 92-93 23.312596703201656 24.99310013646564 26.506970739172846 25.187332421159862 94-95 22.932342483954088 25.24247045235914 26.7339977027491 25.091189360937673 96-97 24.7554850846579 24.793868108704338 25.253455902119974 25.197190904517786 98-99 25.30266066706423 24.04665202908241 25.038220358891884 25.612466944961476 100 24.83094025988425 24.602639938079466 25.569877071838604 24.996542730197678 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2103.0 1 2992.0 2 2460.0 3 940.0 4 748.0 5 766.0 6 991.0 7 1475.5 8 2191.5 9 2320.5 10 1874.5 11 1689.5 12 1815.5 13 2049.5 14 2379.0 15 2811.0 16 3270.5 17 3700.0 18 4257.0 19 5094.0 20 6231.0 21 7633.5 22 9338.0 23 11400.0 24 14078.0 25 17602.0 26 22466.5 27 28049.0 28 33319.5 29 40479.0 30 48604.5 31 55125.0 32 62081.5 33 69488.5 34 77056.0 35 82744.5 36 87349.0 37 93787.5 38 97040.0 39 98296.5 40 100043.0 41 102093.0 42 106335.5 43 118814.0 44 135935.0 45 152124.0 46 180866.5 47 228085.5 48 374257.5 49 547600.0 50 705422.0 51 638836.0 52 384083.5 53 277012.0 54 231510.0 55 196296.5 56 175373.0 57 155859.5 58 144986.0 59 133244.5 60 113420.5 61 94039.0 62 77549.0 63 61428.5 64 46254.0 65 37213.0 66 28827.0 67 20920.0 68 15993.0 69 12983.0 70 11290.0 71 9813.0 72 8172.0 73 8229.0 74 6861.0 75 5233.5 76 4080.5 77 2782.0 78 1982.0 79 1330.0 80 928.5 81 714.5 82 562.5 83 410.5 84 274.0 85 184.5 86 135.0 87 91.5 88 65.5 89 59.0 90 53.5 91 46.0 92 44.5 93 33.5 94 24.5 95 21.5 96 16.5 97 11.0 98 5.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08681867808851935 2 0.28871072238419176 3 0.2935998032369914 4 0.3013683427515627 5 0.3272384791659168 6 0.1736823477186289 7 0.19143900946622036 8 0.15694549424708154 9 0.20849080372889894 10-11 0.2293593804364779 12-13 0.24584128183901427 14-15 0.17264754226205473 16-17 0.1624269637308186 18-19 0.17943376645190703 20-21 0.21158772150835642 22-23 0.17530204321587542 24-25 0.1997249517090787 26-27 0.19852517726667387 28-29 0.22791965110559215 30-31 0.261933256547769 32-33 0.23034919435146192 34-35 0.15573072262414667 36-37 0.15020426159881942 38-39 0.11691801941235047 40-41 0.10809967726067499 42-43 0.12381672245617825 44-45 0.13890388606941895 46-47 0.20453904665922806 48-49 0.2102604710314461 50-51 0.21705419381156343 52-53 0.14349302331161742 54-55 0.08666120769295373 56-57 0.12641123468787868 58-59 0.14549514691238047 60-61 0.12200206361204094 62-63 0.14349302331161742 64-65 0.11799031782024978 66-67 0.12212953964654645 68-69 0.10736481541470204 70-71 0.13354239402992238 72-73 0.15831773626558207 74-75 0.19735539718532918 76-77 0.17789655544757585 78-79 0.20237945266289936 80-81 0.10565513683427515 82-83 0.14722732126360244 84-85 0.17786656108651572 86-87 0.19191142065291725 88-89 0.17302997036557127 90-91 0.17659929933172563 92-93 0.125496406675545 94-95 0.3648364107547781 96-97 0.36530132335121 98-99 0.4121225209660584 100 0.4459261658808144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6667920.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.31788118433771 #Duplication Level Percentage of deduplicated Percentage of total 1 79.96959567297385 28.243566783376174 2 10.954771296868245 7.7379862212877155 3 3.3438387294159213 3.5429189683529483 4 1.5286616716771046 2.1595636516537215 5 0.8785227790030748 1.5513781563282387 6 0.5756559380354135 1.2198568813555921 7 0.40306712341624207 0.9964833741889334 8 0.29057098691294275 0.8209881273125648 9 0.23815447320134514 0.7570000249239281 >10 1.5117041903661061 10.221553762138681 >50 0.14846717791024247 3.6628930245954767 >100 0.13022077135945478 9.395314508412035 >500 0.014601377498782566 3.497069692358469 >1k 0.009520778714321472 6.099235564511536 >5k 9.81963723898408E-4 2.4067926275107228 >10k+ 0.0016650689231320836 17.68739863169327 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 139283 2.0888522957683957 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 116821 1.75198562670218 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 71208 1.0679192311845374 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 57908 0.8684567301347347 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 55307 0.8294490635760478 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 53481 0.8020642119281576 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 52395 0.785777273872512 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 52273 0.7839476178478445 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 44975 0.6744981943394641 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 43785 0.6566515495086924 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 38022 0.5702227981139546 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 34261 0.5138184021403976 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 23819 0.35721784304550747 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 23053 0.34573000275948124 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 22553 0.33823141249445104 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 22314 0.33464708634776663 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21472 0.3220194603414558 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 19144 0.2871060240674753 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 17414 0.2611609017504709 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 17381 0.2606659947929789 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 17288 0.2592712570036833 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 15792 0.23683547493071302 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 15063 0.22590253032429902 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 14763 0.2214033761652809 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 14755 0.22128339872104047 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 14711 0.22062352277771777 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14445 0.21663427275672173 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 13825 0.20733602082808433 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 13475 0.2020870076425632 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 13156 0.19730290705347395 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 12232 0.18344551224369818 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 12058 0.18083600283146767 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 11807 0.17707171051842255 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 11490 0.1723176042903934 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 10518 0.15774034481517474 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 10418 0.1562406267621687 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10211 0.1531362103924462 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 10126 0.1518614500473911 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 10008 0.15009178274484397 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 9489 0.14230824604974265 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 9370 0.14052358156666545 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 9276 0.13911384659683979 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 8555 0.12830087943466628 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 8479 0.1271610937143817 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 8275 0.12410166888624939 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 8133 0.12197206925098081 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8074 0.12108723559970726 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 7851 0.1177428643415038 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 7528 0.1128987750302943 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 6781 0.10169588117433923 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.549520690110259E-4 0.0 0.0 0.0 0.0 2 3.44935152191388E-4 0.0 0.0 1.4997180530060348E-5 0.0 3 3.5993233272144836E-4 0.0 0.0 1.4997180530060348E-5 0.0 4 5.398984990821725E-4 0.0 0.0 1.4997180530060348E-5 0.0 5 0.0021295996352685693 0.0 0.0 8.998308318036209E-5 0.0 6 0.004364179534247561 0.0 0.0 1.1997744424048279E-4 0.0 7 0.004859086491739553 0.0 0.0 1.3497462477054313E-4 0.0 8 0.005473970893472027 0.0 0.0 1.3497462477054313E-4 0.0 9 0.005968877850964019 0.0 0.0 1.6496898583066383E-4 0.0 10-11 0.010640499586077816 0.0 0.0 2.0996052742084487E-4 0.0 12-13 0.02108603582526485 0.0 0.0 3.8992669378156905E-4 0.0 14-15 0.03270885073606162 0.0 0.0 5.099041380220518E-4 0.0 16-17 0.03702054013845397 0.0 0.0 7.798533875631381E-4 7.498590265030174E-6 18-19 0.03894017924630169 0.0 0.0 0.001072298407899315 2.9994361060120696E-5 20-21 0.045861378060924544 0.0 0.0 0.0019946250104980264 2.9994361060120696E-5 22-23 0.04853837478554032 0.0 0.0 0.0058938919483137175 4.4991541590181045E-5 24-25 0.06633252948445692 1.4997180530060348E-5 0.0 0.013579946969969645 4.4991541590181045E-5 26-27 0.12000743860154292 1.4997180530060348E-5 0.0 0.027744783980611643 5.249013185521122E-5 28-29 0.1313378084920035 1.4997180530060348E-5 0.0 0.0839242222462177 7.498590265030174E-5 30-31 0.13400730662635424 1.4997180530060348E-5 0.0 0.21201514115346315 7.498590265030174E-5 32-33 0.135934444324467 1.4997180530060348E-5 0.0 0.41290237435362154 8.998308318036209E-5 34-35 0.1373966694261479 1.4997180530060348E-5 0.0 0.6733359128483845 1.0498026371042244E-4 36-37 0.14008866333129372 1.4997180530060348E-5 0.0 1.0400544697596852 1.0498026371042244E-4 38-39 0.14290813327094506 1.4997180530060348E-5 0.0 1.5860118297760022 1.1997744424048279E-4 40-41 0.14568261166900623 1.4997180530060348E-5 0.0 2.401033605682132 1.1997744424048279E-4 42-43 0.15256631753230393 1.4997180530060348E-5 0.0 2.9635703487744305 1.1997744424048279E-4 44-45 0.16000491907521386 1.4997180530060348E-5 0.0 3.5116498098357507 1.1997744424048279E-4 46-47 0.16298935800069586 1.4997180530060348E-5 0.0 4.135742480413682 1.1997744424048279E-4 48-49 0.1646615436297976 1.4997180530060348E-5 0.0 4.673796326290657 1.4997180530060348E-4 50-51 0.16798341911720596 1.4997180530060348E-5 0.0 5.264182833627277 1.4997180530060348E-4 52-53 0.16928817382332123 2.2495770795090522E-5 0.0 6.002006622754922 1.4997180530060348E-4 54-55 0.17320243794166695 2.9994361060120696E-5 0.0 6.610247273512579 1.4997180530060348E-4 56-57 0.18154836890664555 2.9994361060120696E-5 0.0 7.219942650781653 1.4997180530060348E-4 58-59 0.18477276272060852 2.9994361060120696E-5 0.0 7.844740188844497 1.4997180530060348E-4 60-61 0.1867373933700464 3.749295132515087E-5 0.0 8.369019124404613 1.4997180530060348E-4 62-63 0.18850706067259354 4.4991541590181045E-5 0.0 8.895015237135418 1.4997180530060348E-4 64-65 0.1895643618999628 4.4991541590181045E-5 0.0 9.479702815870617 1.4997180530060348E-4 66-67 0.19121405175826944 4.4991541590181045E-5 0.0 10.061510935944042 1.4997180530060348E-4 68-69 0.19303620919267178 4.4991541590181045E-5 0.0 10.764556263422477 1.4997180530060348E-4 70-71 0.19546575243854156 4.4991541590181045E-5 0.0 11.639994181093954 1.4997180530060348E-4 72-73 0.20159959927533624 4.4991541590181045E-5 0.0 12.306213931780825 1.4997180530060348E-4 74-75 0.20716355325198862 4.4991541590181045E-5 0.0 12.938802505129036 1.4997180530060348E-4 76-77 0.20873075861737994 4.4991541590181045E-5 0.0 13.599713253908266 1.4997180530060348E-4 78-79 0.21009550204561545 4.4991541590181045E-5 0.0 14.199547984978825 1.4997180530060348E-4 80-81 0.2122775918127392 5.998872212024139E-5 0.0 14.885031614056558 1.4997180530060348E-4 82-83 0.2134173775330238 5.998872212024139E-5 0.0 15.717180170128016 1.6496898583066383E-4 84-85 0.217196667026599 5.998872212024139E-5 0.0 16.45391666366723 1.6496898583066383E-4 86-87 0.22491271640931504 5.998872212024139E-5 0.0 17.156219630709426 1.6496898583066383E-4 88 0.22660739780921188 5.998872212024139E-5 0.0 17.717774058477005 1.6496898583066383E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 8595 0.0 31.277905 1 ATCTACA 9745 0.0 27.515509 2 TCTACAC 11450 0.0 25.345959 3 TATCACG 375 0.0 25.088995 2 TACACTC 11875 0.0 24.242617 5 AGTACTC 3405 0.0 23.761456 5 CTACACT 13045 0.0 22.898705 4 CACTCTT 14000 0.0 20.71597 7 ACACTCT 14265 0.0 20.298025 6 GTATAAG 1530 0.0 19.694092 1 AAGTACT 4290 0.0 19.299084 4 ACTCTTT 15910 0.0 19.144894 8 AAAGTAC 4210 0.0 18.099306 3 AGTACTT 29850 0.0 17.744043 12-13 GTATAAT 1315 0.0 17.543562 1 GTACTAG 1400 0.0 16.81471 1 GTGATCG 1045 0.0 16.643074 8 GAGTACT 29380 0.0 16.636217 12-13 GTATTAG 2015 0.0 16.5894 1 ATGGGTA 4260 0.0 16.231903 5 >>END_MODULE