##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765325_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8001033 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22354738444398 33.0 31.0 34.0 31.0 34.0 2 32.24584675503775 34.0 31.0 34.0 31.0 34.0 3 32.283657122774024 34.0 31.0 34.0 30.0 34.0 4 35.747095156337934 37.0 35.0 37.0 35.0 37.0 5 35.758098485533054 37.0 35.0 37.0 35.0 37.0 6 35.87467093311576 37.0 37.0 37.0 35.0 37.0 7 35.82213484183855 37.0 36.0 37.0 35.0 37.0 8 35.86917076832454 37.0 37.0 37.0 35.0 37.0 9 37.561441753833535 39.0 38.0 39.0 35.0 39.0 10-11 37.488209522445416 39.0 38.0 39.0 35.0 39.0 12-13 37.47430856740623 39.0 38.0 39.0 35.0 39.0 14-15 38.82868574595305 41.0 38.5 41.0 35.0 41.0 16-17 38.7627620708476 40.0 38.5 41.0 35.0 41.0 18-19 38.750204817302965 40.0 38.5 41.0 35.0 41.0 20-21 38.75373823605027 40.0 39.0 41.0 35.0 41.0 22-23 38.695029629299114 40.0 39.0 41.0 35.0 41.0 24-25 38.637815829531014 41.0 38.0 41.0 35.0 41.0 26-27 38.49044535874305 40.0 38.0 41.0 34.0 41.0 28-29 38.33938617675993 40.0 38.0 41.0 34.0 41.0 30-31 38.09049031543802 40.0 38.0 41.0 34.0 41.0 32-33 37.76321944928861 40.0 37.0 41.0 33.0 41.0 34-35 37.66059957507987 40.0 37.0 41.0 33.0 41.0 36-37 37.54110250513902 40.0 37.0 41.0 33.0 41.0 38-39 37.341508715187146 40.0 37.0 41.0 32.5 41.0 40-41 37.06932429849996 40.0 37.0 41.0 31.5 41.0 42-43 36.79048099164196 40.0 36.0 41.0 31.0 41.0 44-45 36.36788693409963 40.0 36.0 41.0 30.0 41.0 46-47 35.92904172498726 39.0 35.0 41.0 29.5 41.0 48-49 35.55602914023727 39.0 35.0 41.0 27.5 41.0 50-51 34.327376977447784 38.0 34.0 40.0 25.5 40.5 52-53 34.29491841865919 38.0 34.0 39.5 25.0 40.0 54-55 35.10937462700129 39.0 35.0 40.5 26.0 41.0 56-57 35.10278397801884 39.0 35.0 41.0 26.0 41.0 58-59 34.901876670174964 39.0 35.0 41.0 26.0 41.0 60-61 34.50254217174208 38.0 35.0 40.0 25.0 41.0 62-63 34.06145637694533 37.0 34.0 40.0 23.0 41.0 64-65 33.79906300848903 37.0 34.0 40.0 22.0 41.0 66-67 33.44128939350706 36.5 34.0 39.0 22.0 41.0 68-69 33.16650712476752 36.0 34.0 39.0 21.0 41.0 70-71 32.72666991874674 35.0 34.0 38.5 20.0 40.5 72-73 32.267574636925005 35.0 33.5 37.0 18.0 39.0 74-75 31.863467317282655 35.0 33.0 37.0 13.5 39.0 76-77 31.45701205081894 35.0 33.0 36.0 9.5 38.5 78-79 31.116927089289597 35.0 33.0 36.0 6.5 37.0 80-81 30.81138522988219 35.0 32.5 35.5 2.0 37.0 82-83 30.566011851219713 35.0 32.0 35.0 2.0 36.5 84-85 30.297944027977387 35.0 32.0 35.0 2.0 36.0 86-87 30.02742220910725 35.0 32.0 35.0 2.0 36.0 88-89 29.787311400915357 35.0 31.5 35.0 2.0 35.5 90-91 29.520078032424063 35.0 31.0 35.0 2.0 35.0 92-93 29.17941920749483 35.0 31.0 35.0 2.0 35.0 94-95 28.856466908710413 35.0 31.0 35.0 2.0 35.0 96-97 28.52525805105416 35.0 30.0 35.0 2.0 35.0 98-99 28.16261381499114 35.0 29.5 35.0 2.0 35.0 100 27.55338216952736 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.47518684919683807 1101 2 0.5561512565029645 1101 3 0.5750462820435303 1101 4 0.5193066702970128 1101 5 0.5837452429996333 1101 6 0.5341550362425735 1101 7 0.542493018677817 1101 8 0.4883194119639853 1101 9 0.5559736405785003 1101 10-11 0.536113885566273 1101 12-13 0.6062387564349763 1101 14-15 0.7549647701646407 1101 16-17 0.7703458285286828 1101 18-19 0.7087978651820706 1101 20-21 0.7241775028240482 1101 22-23 0.7507045991085803 1101 24-25 0.6794239305660668 1101 26-27 0.8176036520654577 1101 28-29 0.8033301304181251 1101 30-31 0.8277763771408146 1101 32-33 0.7870077002267237 1101 34-35 0.8645693008378359 1101 36-37 0.8354119785174561 1101 38-39 0.8184047422914418 1101 40-41 0.8590655669264748 1101 42-43 0.8422951931275477 1101 44-45 0.9079851993839156 1101 46-47 0.9684197264926766 1101 48-49 0.9712890406458285 1101 50-51 0.8817346224981293 1101 52-53 0.9852530280005709 1101 54-55 1.1394497929657277 1101 56-57 1.181787203233121 1101 58-59 1.1578685748378703 1101 60-61 1.1561820499597388 1101 62-63 1.1874273460173015 1101 64-65 1.1619026130612653 1101 66-67 1.1120531169334242 1101 68-69 1.0599850194138085 1101 70-71 1.0167994101797078 1101 72-73 0.977786345214362 1101 74-75 0.9355872207278786 1101 76-77 0.9179820809625205 1101 78-79 0.800370274600489 1101 80-81 0.8691857259267621 1101 82-83 0.8499543359070643 1101 84-85 0.865090380524947 1101 86-87 0.8974894487961542 1101 88-89 0.8708962702489238 1101 90-91 0.8246408904359512 1101 92-93 0.743545326893571 1101 94-95 0.7273594716091942 1101 96-97 0.6210834963986045 1101 98-99 0.6535729042044913 1101 100 0.7071807205577763 1102 1 0.49383692009224234 1102 2 0.6005788164208568 1102 3 0.6256683993351473 1102 4 0.5206156787055605 1102 5 0.4943332699838692 1102 6 0.4731384289845977 1102 7 0.5233055099402151 1102 8 0.4845154626097212 1102 9 0.5504673611315596 1102 10-11 0.5309907158400833 1102 12-13 0.5627771445921255 1102 14-15 0.7463102922957532 1102 16-17 0.7358828335181258 1102 18-19 0.7670698356819727 1102 20-21 0.773390495243099 1102 22-23 0.7628634613978065 1102 24-25 0.7776582414742634 1102 26-27 0.7957927053450078 1102 28-29 0.7861271325554995 1102 30-31 0.770033635689515 1102 32-33 0.8316520927661912 1102 34-35 0.8287633836144082 1102 36-37 0.8642285562461893 1102 38-39 0.8525614064170242 1102 40-41 0.8771090976266578 1102 42-43 0.9100411480553845 1102 44-45 0.8857620199360525 1102 46-47 0.8639204951215262 1102 48-49 0.9268251014839137 1102 50-51 0.9467172537554802 1102 52-53 0.9765389453248474 1102 54-55 1.0249152553771594 1102 56-57 1.0356849373539418 1102 58-59 1.066199701882823 1102 60-61 1.0121424746093197 1102 62-63 0.9393337752957365 1102 64-65 0.9802865565488972 1102 66-67 0.9582658653299774 1102 68-69 0.9202090858596463 1102 70-71 0.8322350239607275 1102 72-73 0.8768421098186607 1102 74-75 0.8104791966461313 1102 76-77 0.8257312661767209 1102 78-79 0.7691133317961665 1102 80-81 0.7872909066280833 1102 82-83 0.8292048310261926 1102 84-85 0.7640535790743712 1102 86-87 0.771555320302479 1102 88-89 0.802710850691323 1102 90-91 0.811707683960698 1102 92-93 0.8802363089594571 1102 94-95 0.8746497432362155 1102 96-97 0.8994789882300154 1102 98-99 0.8435668470673612 1102 100 0.9198996719166104 1103 1 0.5565175157801718 1103 2 0.572889442614759 1103 3 0.5551075339576528 1103 4 0.459877285954839 1103 5 0.5033986017323073 1103 6 0.46157798560104624 1103 7 0.4747832940532746 1103 8 0.44928998577267976 1103 9 0.5662159171713839 1103 10-11 0.5904102970030465 1103 12-13 0.5456390366067794 1103 14-15 0.7316093120457126 1103 16-17 0.7268407597561506 1103 18-19 0.6887665734433384 1103 20-21 0.7204592480416281 1103 22-23 0.7378276885529544 1103 24-25 0.6985613085004374 1103 26-27 0.7657106581267925 1103 28-29 0.6976661218961269 1103 30-31 0.7081231950440952 1103 32-33 0.8620356562633873 1103 34-35 0.7745213781445983 1103 36-37 0.7746139236869354 1103 38-39 0.794835921526662 1103 40-41 0.8327910366082705 1103 42-43 0.8813992276286271 1103 44-45 0.9104712145718921 1103 46-47 1.0018471841442036 1103 48-49 1.0340021553219643 1103 50-51 0.9683788790441881 1103 52-53 0.9413846598314493 1103 54-55 1.0079073534601477 1103 56-57 1.0606383086345161 1103 58-59 1.0968102401344808 1103 60-61 1.0859613908902332 1103 62-63 1.0627912908144879 1103 64-65 1.0269246178249958 1103 66-67 1.0407723160388258 1103 68-69 0.9774424807203488 1103 70-71 0.9751516141208683 1103 72-73 0.9042358945888722 1103 74-75 0.9424982926771399 1103 76-77 0.9144015342878227 1103 78-79 0.896493264856975 1103 80-81 0.8576709910913003 1103 82-83 0.8163130128765879 1103 84-85 0.861701687671772 1103 86-87 0.9061261589107339 1103 88-89 0.9097633679979076 1103 90-91 0.8621945232261048 1103 92-93 0.929561601858687 1103 94-95 0.7983360374529624 1103 96-97 0.873963817386894 1103 98-99 0.7924742693286682 1103 100 0.8267236682314127 1104 1 0.45209254640629126 1104 2 0.5426960751815173 1104 3 0.5617421609921038 1104 4 0.4800479259690249 1104 5 0.4897731306344113 1104 6 0.44369495425617345 1104 7 0.46568272741885863 1104 8 0.41890789238013326 1104 9 0.5152835779211955 1104 10-11 0.5541525307284729 1104 12-13 0.5365905036461669 1104 14-15 0.6655290982452513 1104 16-17 0.6268902532033636 1104 18-19 0.6621295700678829 1104 20-21 0.6290123197550344 1104 22-23 0.6681421440226032 1104 24-25 0.7096439893642739 1104 26-27 0.6783196054604019 1104 28-29 0.6753219375810247 1104 30-31 0.6991601635353248 1104 32-33 0.7073360322052196 1104 34-35 0.7172146270534441 1104 36-37 0.6684448838984451 1104 38-39 0.7509213855189572 1104 40-41 0.7060898603194659 1104 42-43 0.7163455814377002 1104 44-45 0.6879088517163297 1104 46-47 0.6387353831528202 1104 48-49 0.6404907837108098 1104 50-51 0.6305038312686122 1104 52-53 0.6959324087751284 1104 54-55 0.728254638724664 1104 56-57 0.7323328095121795 1104 58-59 0.7006717123304114 1104 60-61 0.6876045077833908 1104 62-63 0.7091825828708949 1104 64-65 0.7235711675358871 1104 66-67 0.709271141623006 1104 68-69 0.6125756406861385 1104 70-71 0.6118725444759221 1104 72-73 0.5930806592345377 1104 74-75 0.5930310788667263 1104 76-77 0.546234260951838 1104 78-79 0.5709020505291633 1104 80-81 0.6069517148100481 1104 82-83 0.596625015915496 1104 84-85 0.5991964857676599 1104 86-87 0.6262633428529938 1104 88-89 0.6018965788852917 1104 90-91 0.5471036340355901 1104 92-93 0.6271286617922307 1104 94-95 0.5662333444030558 1104 96-97 0.5733120795265734 1104 98-99 0.5102271052809542 1104 100 0.6307533104516168 1105 1 0.34670870235892437 1105 2 0.3612737151306291 1105 3 0.37953613811742315 1105 4 0.3528751619054873 1105 5 0.40434343289860664 1105 6 0.38233263742408496 1105 7 0.3562114211597489 1105 8 0.33593718659883365 1105 9 0.3669376733473442 1105 10-11 0.4506900870995949 1105 12-13 0.4655477655752023 1105 14-15 0.5785402544307274 1105 16-17 0.5977614504761988 1105 18-19 0.5863191904293146 1105 20-21 0.5102516357763776 1105 22-23 0.537567788590259 1105 24-25 0.5637578024354397 1105 26-27 0.551473827177432 1105 28-29 0.5999840032039359 1105 30-31 0.6106115205206208 1105 32-33 0.6156667955574164 1105 34-35 0.6103473057144058 1105 36-37 0.6892066408209061 1105 38-39 0.6244106689153242 1105 40-41 0.6597187027108049 1105 42-43 0.6417411232015482 1105 44-45 0.6443960128204083 1105 46-47 0.534089555995628 1105 48-49 0.5887885273150673 1105 50-51 0.5640585914397178 1105 52-53 0.4669184559027926 1105 54-55 0.5691049232554803 1105 56-57 0.575610642864028 1105 58-59 0.5505026658342587 1105 60-61 0.5745752134817508 1105 62-63 0.5887883488013586 1105 64-65 0.5926044519742675 1105 66-67 0.5441693228891396 1105 68-69 0.5629781252348423 1105 70-71 0.5226118642915978 1105 72-73 0.5288174878583263 1105 74-75 0.5651641793819557 1105 76-77 0.5501290626979518 1105 78-79 0.5802017952561194 1105 80-81 0.6189930042783338 1105 82-83 0.5658589931768354 1105 84-85 0.5724278048453577 1105 86-87 0.5177679970380318 1105 88-89 0.5568158648904173 1105 90-91 0.5623160666650726 1105 92-93 0.5314204494263244 1105 94-95 0.4051627364987489 1105 96-97 0.44924343592135685 1105 98-99 0.4521847500013685 1105 100 0.48124207130934593 1106 1 0.29405575143415064 1106 2 0.2991017322854148 1106 3 0.3465721124478023 1106 4 0.36467207686233394 1106 5 0.2911619157195062 1106 6 0.32234849720074976 1106 7 0.32762696276094516 1106 8 0.3444060359697758 1106 9 0.40062635663537094 1106 10-11 0.4007140011402086 1106 12-13 0.4247867073572351 1106 14-15 0.5236858737893115 1106 16-17 0.49871134104449055 1106 18-19 0.5091876843121028 1106 20-21 0.533278765115341 1106 22-23 0.506785763657092 1106 24-25 0.5544694684544709 1106 26-27 0.534102340176382 1106 28-29 0.5336031680555422 1106 30-31 0.5079555167395995 1106 32-33 0.5461509031792673 1106 34-35 0.5216694638350177 1106 36-37 0.49449188877878925 1106 38-39 0.5250167927941973 1106 40-41 0.5618535914681217 1106 42-43 0.520677338481434 1106 44-45 0.5133404180467522 1106 46-47 0.523118815549843 1106 48-49 0.46598274889890234 1106 50-51 0.45076036846587897 1106 52-53 0.5052051929761134 1106 54-55 0.5405757366306077 1106 56-57 0.5779442311045813 1106 58-59 0.5564310488418371 1106 60-61 0.5340780954157367 1106 62-63 0.5383917036265515 1106 64-65 0.5142484678295247 1106 66-67 0.6058963990481061 1106 68-69 0.5674422679306161 1106 70-71 0.5215985044649969 1106 72-73 0.47518387282048025 1106 74-75 0.5097420805470527 1106 76-77 0.5113139553561403 1106 78-79 0.5240882621705083 1106 80-81 0.5388737318229779 1106 82-83 0.5239220011146379 1106 84-85 0.4816837069398865 1106 86-87 0.5358380416210409 1106 88-89 0.504433091743639 1106 90-91 0.5368344032127013 1106 92-93 0.5774310447219868 1106 94-95 0.41574308824054995 1106 96-97 0.49418815095607016 1106 98-99 0.4413971809304975 1106 100 0.5148610665578204 1107 1 0.20530638163744186 1107 2 0.25480933549627593 1107 3 0.2824157957284541 1107 4 0.2831719519176801 1107 5 0.3183009462366897 1107 6 0.26562634341799196 1107 7 0.2507454935872033 1107 8 0.28331967510331424 1107 9 0.32425018993728116 1107 10-11 0.3225672152865755 1107 12-13 0.30656679600217984 1107 14-15 0.3270132594649553 1107 16-17 0.3969565043712677 1107 18-19 0.37531058600915657 1107 20-21 0.3178157903804646 1107 22-23 0.2903855286734611 1107 24-25 0.3022365540875924 1107 26-27 0.3349429327209066 1107 28-29 0.33970884054271977 1107 30-31 0.3300139908366049 1107 32-33 0.24712687434993086 1107 34-35 0.3288016114362975 1107 36-37 0.42553743439234637 1107 38-39 0.45163215177991134 1107 40-41 0.42144986680229124 1107 42-43 0.40495859985249893 1107 44-45 0.3996450374916378 1107 46-47 0.3878120873899533 1107 48-49 0.3648968264347374 1107 50-51 0.4383442457833837 1107 52-53 0.46844340762664416 1107 54-55 0.502267925617069 1107 56-57 0.45552262839350277 1107 58-59 0.4088651898583109 1107 60-61 0.46594542611573786 1107 62-63 0.4716553456097117 1107 64-65 0.5367398641846464 1107 66-67 0.4929163595042212 1107 68-69 0.5200012220781218 1107 70-71 0.4667106258498208 1107 72-73 0.48634607728254053 1107 74-75 0.49580544721358066 1107 76-77 0.5150936845112035 1107 78-79 0.4731450396506567 1107 80-81 0.48885251315229894 1107 82-83 0.4624311264582346 1107 84-85 0.4770358535766803 1107 86-87 0.4240170062025186 1107 88-89 0.44290289240212033 1107 90-91 0.46923009634863533 1107 92-93 0.48189104270533534 1107 94-95 0.46631533474538855 1107 96-97 0.46189934029763435 1107 98-99 0.45110516116732313 1107 100 0.46622515527290886 1108 1 0.22967605904413801 1108 2 0.28945142175662397 1108 3 0.30362478477912447 1108 4 0.2692924763607323 1108 5 0.2695162144757717 1108 6 0.23676622869198383 1108 7 0.3675170750269672 1108 8 0.29275005133135323 1108 9 0.35687551207272605 1108 10-11 0.32348324095276126 1108 12-13 0.38955893557021426 1108 14-15 0.4184144178443958 1108 16-17 0.4116121873638221 1108 18-19 0.4303733860693413 1108 20-21 0.4732327432080652 1108 22-23 0.37839079076417903 1108 24-25 0.49115394187980144 1108 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-1.106643335431599 2316 90-91 -1.0165884004274197 2316 92-93 -1.0089555399518595 2316 94-95 -0.8127168081419818 2316 96-97 -1.0333945951645553 2316 98-99 -0.7803188630681319 2316 100 -0.7923875191255405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23166.0 3 3193.0 4 3109.0 5 3588.0 6 4424.0 7 5473.0 8 7101.0 9 8964.0 10 12157.0 11 18569.0 12 29019.0 13 41008.0 14 42386.0 15 50696.0 16 65286.0 17 87057.0 18 85100.0 19 51323.0 20 45820.0 21 43532.0 22 45316.0 23 51890.0 24 57450.0 25 59968.0 26 65199.0 27 74229.0 28 86039.0 29 101712.0 30 122451.0 31 152098.0 32 194373.0 33 259630.0 34 374146.0 35 595316.0 36 1080829.0 37 2036684.0 38 1711045.0 39 301407.0 40 280.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.40595689831382 17.183807007883136 13.477939651434708 23.93229644236834 2 13.506002840614267 22.145849449449248 36.51724698053131 27.830900729405172 3 19.294897473679672 28.34259017397813 29.252640054628564 23.109872297713633 4 13.074904435125253 19.125698129692456 33.546276338707365 34.25312109647493 5 12.558685328279246 36.59481909045452 33.95236815141404 16.894127429852194 6 30.481145440587394 32.518712839108396 18.966333164904846 18.033808555399368 7 23.97473062195391 32.525747010461835 22.538652915060315 20.96086945252394 8 27.922798160492395 30.67734050730785 21.847125697391512 19.552735634808247 9 28.033388124422736 14.713066892092863 19.3392507709889 37.9142942124955 10-11 23.958492326102128 26.382464371893043 25.831043785269774 23.82799951673505 12-13 27.337777437003023 24.095343262908223 26.16185397501219 22.40502532507656 14-15 26.51150478063848 22.442046683148806 25.340401089884416 25.706047446328302 16-17 22.584455099277132 26.05052972755903 28.772967858830118 22.592047314333726 18-19 24.6007510994459 25.371319459655624 29.560954697964835 20.466974742933644 20-21 23.36762315982899 25.018667221736283 29.22014471887327 22.393564899561454 22-23 22.026806395044655 26.712792505587167 25.500322566631322 25.760078532736852 24-25 22.338163050963303 27.8036691795157 24.685488969933093 25.172678799587906 26-27 23.94577385385919 24.78974833414061 25.589668891819695 25.674808920180503 28-29 22.97315028471467 23.922157426897638 29.305183974609132 23.799508313778556 30-31 25.278464635108815 24.310265724038125 27.881523568773066 22.529746072079995 32-33 20.577598538920775 26.102836728405116 28.616064606157476 24.703500126516634 34-35 20.111319927159442 26.899322014040383 29.19144253642153 23.797915522378645 36-37 23.309196763010178 26.448507134079442 27.079975237183078 23.162320865727303 38-39 24.42687028429211 24.438025226184745 26.62905476350741 24.506049726015732 40-41 22.3695236236395 26.150319370248976 26.86459771171944 24.61555929439209 42-43 25.224741769312313 24.90968002738427 26.49422360736935 23.371354595934065 44-45 24.904995156284524 24.15740908248121 27.57528690746304 23.36230885377123 46-47 22.78060789205832 24.833362084970638 30.053822438863115 22.332207584107934 48-49 23.539160140362757 26.041665231637502 28.774954322238962 21.64422030576078 50-51 21.773162630179897 26.80613309437781 27.107146666008703 24.31355760943359 52-53 20.99165380202857 27.592015720296764 24.71990508547295 26.696425392201718 54-55 22.205337909777022 26.76660406142073 24.526281100160283 26.501776928641966 56-57 23.565328278968316 24.23803326252876 25.348773898733796 26.84786455976913 58-59 23.24449420547586 23.95668871139323 28.09210410624674 24.70671297688417 60-61 23.492020152738466 24.99644938309361 26.73027662027319 24.781253843894735 62-63 20.262946592086784 25.562054097845767 27.88246778453053 26.292531525536923 64-65 20.29343951292492 25.74058244326718 27.760804622848838 26.205173420959067 66-67 23.000275041406372 25.170030146940686 25.993734111290912 25.835960700362033 68-69 23.618159485254168 23.887092975355895 26.027231783572034 26.4675157558179 70-71 22.011016186135965 24.755789435877084 26.696002031862932 26.537192346124023 72-73 23.847317742862696 23.76949578141004 26.798518791587796 25.584667684139472 74-75 23.926218345860896 22.86972691897104 27.483309279996494 25.720745455171574 76-77 22.0772319415894 24.311705670377172 29.00805113907461 24.60301124895882 78-79 22.57589771066616 24.087743802792904 28.679447991148205 24.656910495392736 80-81 21.451119809063478 24.447711685003036 28.096864764829053 26.00430374110443 82-83 21.48279373287592 25.28764829398592 25.917931566938098 27.311626406200062 84-85 22.00875210375071 25.098493249687237 25.948400711261016 26.94435393530104 86-87 22.56250245346739 23.01251298107291 26.91088248881986 27.51410207663984 88-89 22.67201895736496 22.231179847583906 28.394232666400253 26.702568528650882 90-91 23.3938023745126 22.982515323732212 27.45243722726849 26.1712450744867 92-93 21.952263031723827 23.43056761214674 27.958871010026094 26.658298346103344 94-95 21.47006394865543 23.73417574647427 28.33249939132059 26.463260913549714 96-97 23.40030325624149 23.168458240050093 26.830952206535365 26.600286297173053 98-99 23.977856095239925 22.43240601057449 26.505181765576452 27.084556128609137 100 23.153969248104982 22.87964489986069 27.40089982704711 26.565486024987216 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2289.0 1 3401.5 2 2799.0 3 971.0 4 787.0 5 815.5 6 996.0 7 1442.5 8 2147.5 9 2334.5 10 1952.0 11 1863.5 12 2056.5 13 2269.0 14 2651.5 15 3119.5 16 3529.5 17 4083.0 18 4877.5 19 5709.0 20 6632.0 21 7841.5 22 9642.5 23 11787.5 24 14419.0 25 17892.0 26 22479.5 27 28067.0 28 34405.5 29 43065.5 30 53355.0 31 62993.0 32 73449.5 33 83886.0 34 94024.5 35 101369.0 36 107598.0 37 116361.0 38 121014.0 39 122192.0 40 123008.5 41 125336.0 42 131353.5 43 145645.0 44 164758.5 45 183925.5 46 218971.5 47 279042.0 48 457051.0 49 682599.0 50 922548.0 51 851817.5 52 498212.0 53 333274.0 54 264156.0 55 217774.0 56 191298.0 57 168319.5 58 153144.0 59 138053.0 60 116654.0 61 96709.5 62 78838.5 63 61269.5 64 44847.0 65 35232.5 66 27443.5 67 20049.5 68 15734.5 69 12952.0 70 10924.0 71 9292.5 72 7741.0 73 7632.5 74 6385.0 75 5027.0 76 3816.0 77 2471.5 78 1740.5 79 1207.0 80 885.0 81 649.5 82 484.0 83 367.0 84 254.0 85 166.0 86 115.5 87 82.5 88 62.5 89 49.5 90 41.0 91 40.0 92 36.0 93 25.5 94 24.5 95 26.0 96 16.5 97 11.5 98 8.0 99 6.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08757619172424361 2 0.2809262254011451 3 0.2848257218786624 4 0.29331212607172097 5 0.3180214354821434 6 0.16834076299897777 7 0.18455116983019568 8 0.1519303819894256 9 0.20194892334527303 10-11 0.22290246771885577 12-13 0.24023148011013076 14-15 0.16744087919647377 16-17 0.15731718641830372 18-19 0.17424625045291026 20-21 0.2055859537137267 22-23 0.1691781548707523 24-25 0.19424991747940548 26-27 0.19203770313158314 28-29 0.2210902017277019 30-31 0.2554857604011882 32-33 0.22363362330839032 34-35 0.15094300948390038 36-37 0.1454187228074175 38-39 0.1132291292886806 40-41 0.10436777351124536 42-43 0.1197782836291264 44-45 0.1347638486180472 46-47 0.19973045980437779 48-49 0.20364870386111394 50-51 0.21049157027598814 52-53 0.1388258241154611 54-55 0.08450783792542788 56-57 0.12234045278903362 58-59 0.14112552716630467 60-61 0.11828472648469267 62-63 0.13900080152150351 64-65 0.11454145983399892 66-67 0.11884715386125766 68-69 0.10436777351124536 70-71 0.12918956839698073 72-73 0.15354892299531822 74-75 0.19132529512126747 76-77 0.17142786437701232 78-79 0.19700581162457398 80-81 0.10273048492613392 82-83 0.14212539805797578 84-85 0.17260896186779884 86-87 0.1860572253607753 88-89 0.16779083400855865 90-91 0.1708216926489367 92-93 0.12228421005137712 94-95 0.36065343062577043 96-97 0.3611658644577519 98-99 0.4075723722174374 100 0.4409430632269608 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8001033.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.277711294935884 #Duplication Level Percentage of deduplicated Percentage of total 1 78.73343545750089 25.413350989556815 2 11.710873296471824 7.560003745501833 3 3.561724659069908 3.448929607725373 4 1.5924280183238386 2.055997273336949 5 0.8983084316934062 1.44976701060032 6 0.6099257541124898 1.1812204441553402 7 0.4234712518306447 0.9568077965806246 8 0.31401298960722734 0.8108496497121429 9 0.24733076129545814 0.718494381670635 >10 1.5680302302587066 9.61845847258499 >50 0.16085904784681618 3.622376504293777 >100 0.14757060224700047 9.84839537607739 >500 0.018944288135315842 4.189166968486879 >1k 0.010233807286320614 6.070672039703892 >5k 9.374479956896618E-4 2.007700959946864 >10k+ 0.0019139563245330591 21.047808780066273 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 187956 2.3491466664366962 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 173528 2.1688199511238113 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 89085 1.1134187298065137 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 73336 0.9165816463949092 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 70543 0.8816736538894415 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 70310 0.8787615299174495 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 68628 0.8577392444200643 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 64041 0.8004091471688719 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 63780 0.7971470683847949 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 56089 0.7010219805367632 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 55657 0.6956226777217391 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 45328 0.5665268472208526 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 34684 0.4334940250840111 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 31261 0.3907120493066333 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 27769 0.3470676848851892 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 26937 0.3366690276118096 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 24792 0.30985998932887787 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23989 0.2998237852537291 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 23962 0.2994863288277901 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 23463 0.29324963414099153 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 22025 0.2752769548632033 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 20937 0.26167871073647614 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 20712 0.25886657385365114 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 20651 0.25810417229875193 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 18637 0.23293242260093167 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 17760 0.22197133795098709 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 17554 0.21939667040493396 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 17447 0.21805934308732383 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 17391 0.2173594334631541 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 17135 0.21415984660980653 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 16971 0.21211011128188076 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 16853 0.21063530171666583 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 16831 0.21036033722145628 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16472 0.20587341659508215 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 14492 0.18112661202622213 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 13985 0.17478993025025644 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 12977 0.1621915570152004 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 12417 0.15519246077350263 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 12078 0.15095550787004627 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 11975 0.1496681740970197 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 11942 0.1492557273542054 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 11697 0.1461936227484626 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 11664 0.14578117600564827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11558 0.14445634707418406 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 11356 0.14193167307271448 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 10349 0.12934579822380435 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 10214 0.12765851609410936 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 10189 0.12734605644046212 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC 10023 0.12527132434024457 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT 9258 0.11571005893863955 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 9025 0.11279793496664743 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT 8643 0.10802355145891787 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 8489 0.10609879999245098 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 8197 0.10244927123785141 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2498386145888912E-4 0.0 0.0 0.0 0.0 2 2.9996126750133387E-4 0.0 0.0 0.0 0.0 3 3.249580397931117E-4 0.0 0.0 1.2498386145888912E-5 0.0 4 4.3744351510611193E-4 0.0 0.0 2.4996772291777823E-5 1.2498386145888912E-5 5 0.0015373014959443362 0.0 0.0 9.998708916711129E-5 1.2498386145888912E-5 6 0.0028996255858462277 0.0 0.0 9.998708916711129E-5 1.2498386145888912E-5 7 0.0033120723286605615 0.0 0.0 1.2498386145888912E-4 1.2498386145888912E-5 8 0.003699522299183118 0.0 0.0 1.2498386145888912E-4 1.2498386145888912E-5 9 0.004061975497413896 0.0 0.0 1.4998063375066693E-4 4.9993544583555646E-5 10-11 0.007255313157688514 0.0 0.0 2.6246610906366716E-4 6.249193072944456E-5 12-13 0.016329141499603864 0.0 0.0 3.749515843766674E-4 9.998708916711129E-5 14-15 0.026727798772983438 6.249193072944456E-6 0.0 5.124338319814454E-4 1.3748224760477804E-4 16-17 0.030652292022792557 1.2498386145888912E-5 0.0 8.061459064098349E-4 1.6247901989655585E-4 18-19 0.032602040261551224 1.2498386145888912E-5 0.0 0.0011998450700053355 1.7497740604244477E-4 20-21 0.03984485503309385 1.2498386145888912E-5 0.0 0.002262207892405893 1.874757921883337E-4 22-23 0.04299444834185786 1.2498386145888912E-5 0.0 0.006674138201904678 1.9997417833422259E-4 24-25 0.061392072748606336 1.8747579218833367E-5 0.0 0.016835326138512366 1.9997417833422259E-4 26-27 0.11666618547880006 2.4996772291777823E-5 0.0 0.03441430625270512 2.124725644801115E-4 28-29 0.1284959079658839 2.4996772291777823E-5 0.0 0.10612379676474275 2.2497095062600043E-4 30-31 0.1310393295465723 2.4996772291777823E-5 0.0 0.2828572260606849 2.3746933677188932E-4 32-33 0.13264537216631903 2.4996772291777823E-5 0.0 0.5511413338752633 2.749644952095561E-4 34-35 0.13417642446919043 3.749515843766673E-5 0.0 0.8960655455364326 2.87462881355445E-4 36-37 0.1371822863372767 3.749515843766673E-5 0.0 1.377090933133259 2.87462881355445E-4 38-39 0.13988818693786165 3.749515843766673E-5 0.0 2.0719074649485885 2.87462881355445E-4 40-41 0.1424566052908418 3.749515843766673E-5 0.0 3.1012495511517075 2.87462881355445E-4 42-43 0.1486120604676921 3.749515843766673E-5 0.0 3.7878046497246043 2.87462881355445E-4 44-45 0.15544867768949333 4.374435151061119E-5 0.0 4.462348799211302 2.87462881355445E-4 46-47 0.15821707022080775 4.9993544583555646E-5 0.0 5.228174911914499 2.9371207442838946E-4 48-49 0.15998559186045103 4.9993544583555646E-5 0.0 5.886683631976021 3.249580397931117E-4 50-51 0.16282897470864074 4.9993544583555646E-5 0.0 6.602778666204726 3.249580397931117E-4 52-53 0.16411005928859437 4.9993544583555646E-5 0.0 7.50841797552891 3.4995481208488955E-4 54-55 0.1675721122510056 5.62427376565001E-5 0.0 8.252746864061177 3.749515843766674E-4 56-57 0.17528361650301905 6.249193072944456E-5 0.0 8.984652356764432 3.749515843766674E-4 58-59 0.1779332743659475 6.249193072944456E-5 0.0 9.737323918049082 3.749515843766674E-4 60-61 0.179676799233299 6.249193072944456E-5 0.0 10.375878964628694 3.749515843766674E-4 62-63 0.1810828676747115 6.249193072944456E-5 0.0 11.023395354074905 4.124467428143341E-4 64-65 0.18217022726940385 6.249193072944456E-5 0.0 11.735559645860729 4.124467428143341E-4 66-67 0.18357629571081635 6.249193072944456E-5 0.0 12.438456384319375 4.1869593588727855E-4 68-69 0.1852260826820737 6.249193072944456E-5 0.0 13.296758056116005 4.24945128960223E-4 70-71 0.1875320349259902 6.249193072944456E-5 0.0 14.362101743612357 4.24945128960223E-4 72-73 0.19316255788471315 6.249193072944456E-5 0.0 15.162979580261698 4.8743705968966756E-4 74-75 0.19834313894218408 6.249193072944456E-5 0.0 15.908733284814598 4.999354458355565E-4 76-77 0.19983044689354487 8.123950994827793E-5 0.0 16.697487686902427 5.124338319814454E-4 78-79 0.200942803260529 1.1248547531300021E-4 0.0 17.412982798596133 5.124338319814454E-4 80-81 0.20323000792522666 1.1248547531300021E-4 0.0 18.22240953136926 5.186830250543898E-4 82-83 0.20411114414851184 1.1873466838594466E-4 0.0 19.217193579878998 5.374306042732232E-4 84-85 0.20742321647717238 1.3123305453183358E-4 0.0 20.076482624181153 5.374306042732232E-4 86-87 0.21386613453537812 1.4373144067772247E-4 0.0 20.881366193590253 5.686765696379455E-4 88 0.21535969167981184 1.874757921883337E-4 0.0 21.53318202787065 5.874241488567789E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTACA 10645 0.0 31.331232 2 TCTACAC 12685 0.0 28.734865 3 TACACTC 12965 0.0 27.683487 5 CTACACT 14965 0.0 25.335836 4 CACTCTT 15430 0.0 23.489222 7 ACACTCT 15755 0.0 22.974693 6 AGTACTC 3710 0.0 22.695925 5 ACTCTTT 17640 0.0 21.798916 8 GTATAAG 1765 0.0 20.807987 1 CGTTAAC 480 0.0 19.618643 1 TGATCGC 1340 0.0 18.94377 9 AGTACTT 34345 0.0 18.733639 12-13 GTGATCG 1340 0.0 18.242264 8 GAGTACT 33715 0.0 17.919714 12-13 TGGTCGC 21500 0.0 17.834002 94 AAAGTAC 4645 0.0 17.820705 3 GATGTAA 2735 0.0 17.732096 1 AAGTACT 4935 0.0 17.53911 4 CATGGGT 8065 0.0 16.798288 4 ATGTAAG 3410 0.0 16.691982 2 >>END_MODULE